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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
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Vandy - Hyperinsulinemic-Hypoglycemic clamp Resource Report Resource Website |
Li Kang | 10.17504/protocols.io.yycfxsw | Mouse Metabolic Phenotyping Centers | Summary:Mice with catheters implanted in the jugular vein (infusions) and carotid artery (sampling) are used for this procedure (V3002). The hyperinsulinemic hypoglycemic clamp involves a constant rate insulin infusion with a fall in blood glucose that is controlled by feed back from regular glucose measurements. Blood glucose is then clamped at a hypoglycemic level. The hypoglycemic clamp is used to test hypoglycemic counterregulation and the functionality of the hypothalamic-pituitary-adrenal axis. | Vanderbilt University | https://mmpc.org/shared/document.aspx?id=235&docType=Protocol | 1 | 2019 | Li Kang 2019. Vandy - Hyperinsulinemic-Hypoglycemic clamp. protocols.io dx.doi.org/10.17504/protocols.io.yycfxsw | 2021-03-29 03:08:15 | ||
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U Mass - Lipase Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.x3bfqin | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Lipase is a gastrointestinal enzyme that processes fat/lipid digestion. | University of Massachusetts | http://mmpc.org/shared/document.aspx?id=174&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Lipase. protocols.io dx.doi.org/10.17504/protocols.io.x3bfqin | 2021-03-29 03:08:21 | ||
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UC Davis - Gut Microbiome Analysis (454-3K reads) Resource Report Resource Website |
Trina Knotts | 10.17504/protocols.io.ynkfvcw | Mouse Metabolic Phenotyping Centers | The work of Gordon and colleagues (i.e., Nature. 2006 Dec 21;444(7122):1027-31) has shown that obesity can result in marked shifts in the gut microbiome in mice and other models including humans. While the role of the microbiome remains to be fully elucidated, the gut microbiota can no longer be ignored as a potentially important factor when assessing metabolic phenotype. This service involves 16S gene variable region (V1-V3 or V3-V4 directed primers) sequencing by Titanium 454 (3000 avg seq read depth) of feces, cecal, or other GI contents. Sequences can be processed through a bioinformatics pipeline (Qiime) to taxonomically classify them and to assess alpha and beta diversity of the community. In addition, Principal Components Analysis (PCA) or partial least squares- discriminant analysis (PLS-DA) can leverage variances in the relative microbial abundances to better understand how specific microbes contribute to separation by group. Correlational analyses can identify which variables of host metadata associate with specific microbes. The Core’s gut microbiome assay will employ this approach to uncover unique microbiota fingerprints in test mice. One caveat is that with fecal samples, patterns are only a surrogate for actual gut microbiota patterns, and may not exactly reflect the intestinal populations. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=106&docType=Protocol | 1 | 2019 | Trina Knotts 2019. UC Davis - Gut Microbiome Analysis (454-3K reads). protocols.io dx.doi.org/10.17504/protocols.io.ynkfvcw | 2021-03-29 03:08:29 | ||
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Yale - Total Cholesterol Resource Report Resource Website |
John Stack, Gary Cline | 10.17504/protocols.io.y3rfym6 | Mouse Metabolic Phenotyping Centers | Procedure used to determine the total concentration of cholesterol present in blood, serum, and plasma. Cholesterol esters are hydrolysed by cholesterol esterase. Cholesterol is then oxidized by cholesterol oxidase with formation of hydrogen peroxide. Peroxidase uses the hydrogen peroxide, phenol, and 4-aminotipyrine to form a quinoneimine dye which is measured at 500 nm. | Yale University, Yale University | https://mmpc.org/shared/document.aspx?id=212&docType=Protocol | 1 | 2019 | John Stack, Gary Cline 2019. Yale - Total Cholesterol. protocols.io dx.doi.org/10.17504/protocols.io.y3rfym6 | 2021-03-29 03:09:35 | ||
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UC Davis - Leptin Signaling pathway Resource Report Resource Website |
Fawaz G. Haj | 10.17504/protocols.io.ybqfsmw | Mouse Metabolic Phenotyping Centers | Summary: This test is designated to determine if rodents exhibit activation of leptin signaling pathway through evaluation of the phosphorylation state of JAK2 and STAT3 proteins. |
RRID:AB_330403 RRID:AB_10691469 RRID:AB_330385 RRID:AB_330426 |
University of California, Davis | https://mmpc.org/shared/document.aspx?id=247&docType=Protocol | 1 | 2019 | Fawaz G. Haj 2019. UC Davis - Leptin Signaling pathway. protocols.io dx.doi.org/10.17504/protocols.io.ybqfsmw | 2021-03-29 03:09:39 | |
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Vandy - Biliopancreatic Diversion in Mice Resource Report Resource Website |
Vance L. Albaugh | 10.17504/protocols.io.zb4f2qw | Mouse Metabolic Phenotyping Centers | This is the protocol for the biliopancreatic diversion procedure in the mouse. This procedure is historically thought to be a malabsorptive procedure and has several variations in the human. In brief, the biliary and pancreatic secretions are physically separated from gastrointestinal chyme flow until a point near the terminal small bowel. In theory this leads to significant malabsorption, though when this procedure is done clinically many times a gastric restriction component is also added. | Vanderbilt University | https://mmpc.org/shared/document.aspx?id=292&docType=Protocol | 1 | 2019 | Vance L. Albaugh 2019. Vandy - Biliopancreatic Diversion in Mice. protocols.io dx.doi.org/10.17504/protocols.io.zb4f2qw | 2021-03-29 03:09:37 | ||
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UC Davis - Total Cholesterol (TC) Protocol Resource Report Resource Website |
Peter Havel | 10.17504/protocols.io.ygeftte | Mouse Metabolic Phenotyping Centers | Cholesterol esters are enzymatically hydrolysed by cholesterol esterase to cholesterol and free fatty acids. Free cholesterol, including that originally present, is then oxidized by cholesterol oxidase to cholest-4-en-3one and hydrogen peroxide. The hydrogen peroxide combines with HBA and 4-aminoantipyrine to form a chromophore (quinoneimine dye) which may be quantitated at 500-550nm. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=92&docType=Protocol | 1 | 2019 | Peter Havel 2019. UC Davis - Total Cholesterol (TC) Protocol. protocols.io dx.doi.org/10.17504/protocols.io.ygeftte | 2021-03-29 03:09:38 | ||
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UC Davis - Electrocardiography Resource Report Resource Website |
Anil Singapuri | 10.17504/protocols.io.yidfua6 | Mouse Metabolic Phenotyping Centers | Cardiac hypertrophy is one of the most common causes of heart failure. The development of cardiac hypertrophy and failure can be monitored using electrocardiography (ECG) in conscious animals. ECG is used to measure the rate and regularity of heartbeats as well as the size and position of the chambers, the presence of any damage to the heart, and the effects of drugs or devices used to regulate the heart. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=114&docType=Protocol | 1 | 2019 | Anil Singapuri 2019. UC Davis - Electrocardiography. protocols.io dx.doi.org/10.17504/protocols.io.yidfua6 | 2021-03-29 03:09:48 | ||
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U Mass - Cytokines Panel II - multiplex Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.xvvfn66 | Mouse Metabolic Phenotyping Centers | Summary: This experiment provides the quantification of multiple cytokines and chemokines using multiplexed-Luminex technology based on beads containing specific antibodies. Serum cytokine levels reflect chronic or acute inflammation, and circulating cytokines and chemokines are altered in obesity. Cytokines Panel II include IL-16 (interleukin-16), IL-17E/IL-25, IL-21, IL-22, IL-28B, EPO (erythropoietin), Exodus-2 (CCL-21), Fractalkine (CX3CL1), MCP-5 (monocyte chemotactic protein-5; CCL-12), MIP-3α (macrophage inflammatory protein 3-alpha; CCL-20), MIP-3β(macrophage inflammatory protein 3-beta; CCL-19), and TARC (thymus and activation- regulated; CCL-17). A service can be requested for all or any combination of listed cytokines/chemokines for customized multiplexed Luminex assay. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=191&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Cytokines Panel II - multiplex. protocols.io dx.doi.org/10.17504/protocols.io.xvvfn66 | 2021-03-29 03:09:48 | ||
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UC Davis - Glutathione Resource Report Resource Website |
Peter Havel | 10.17504/protocols.io.ykcfusw | Mouse Metabolic Phenotyping Centers | Summary:Cayman's GSH assay kit utilizes a carefully optimized enzymatic recycling method, using glutathione reductase for the quantification of GSH. The sulfhydryl group of GSH reacts with DTNB (5,5'-dithio-bis-2-nitrobenzoic acid, Ellman's reagent) and produces a yellow colored 5-thio-2-nitrobenzoic acid (TNB). The mixed disulfide, GSTNB (between GSH and TNB) that is concomitantly produced, is reduced by glutathione reductase to recycle the GSH and produce more TNB. The rate of TNB production is directly proportional to this recycling reaction which in turn is directly proportional to the concentration of GSH in the sample. Measurement of the absorbance of TNB at 405 or 412 nm provides an accurate estimation of GSH in the sample. GSH is easily oxidized to the disulfide dimer GSSG. Because of the use of glutathione reductase in the Cayman GSH assay kit, both GSH and GSSG are measured and the assay reflects total glutathione. The kit can also be used to measure only GSSG by following an alternative protocol. GSH measurement can be done in plasma, tissue samples, and cultured cells using this kit. Nearly all samples require deproteination before assay. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=125&docType=Protocol | 1 | 2019 | Peter Havel 2019. UC Davis - Glutathione. protocols.io dx.doi.org/10.17504/protocols.io.ykcfusw | 2021-03-29 03:08:33 | ||
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UC Davis - Superoxide Dismutase Resource Report Resource Website |
Peter Havel | 10.17504/protocols.io.ywdfxa6 | Mouse Metabolic Phenotyping Centers | Significant amounts of superoxide dismutase (SOD) in cellular and extracellular environments are crucial for the prevention of diseases linked to oxidative stress. Mutations in SOD account for approximately 20% of familial amyotrophic lateral sclerosis (ALS) cases. SOD also appears to be important in the prevention of other neurodegenerative disorders such as Alzheimer's, Parkinson's, and Huntington's Diseases. The reaction catalyzed by SOD is extremely fast, having a turnover of 2 x 10^6 M-1sec-1 and the presence of sufficient amounts of the enzyme in cells and tissues typically keeps the concentration of superoxide very low. Quantification of SOD activity is therefore essential in order to fully characterize the antioxidant capabilities of a biological system. The Cayman Chemical SOD Assay kit is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates, and cell lysates. SOD activity is assessed by measuring the dismutation of superoxide radicals generated by xanthine oxidase and hypoxanthine in a convenient 96 well format. A key feature of the kit is the inclusion of a quality-controlled SOD standard. The standard curve generated using this enzyme provides a means to accurately quantify the activity of all three types of SOD (Cu/Zn-, Mn-, and Fe-SOD). Each kit contains sufficient reagents to assay 41 samples in duplicate and includes assay buffer, sample buffer, radical detector, SOD (standard), xanthine oxidase, a 96 well plate, and complete instructions. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=129&docType=Protocol | 1 | 2019 | Peter Havel 2019. UC Davis - Superoxide Dismutase. protocols.io dx.doi.org/10.17504/protocols.io.ywdfxa6 | 2021-03-29 03:08:32 | ||
|
U Mass - Acute lipid infusion Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.xm5fk86 | Mouse Metabolic Phenotyping Centers | Triglyceride emulsion and heparin will be intravenously infused for 5 hours to acutely raise circulating fatty acids levels in awake mice. Acute lipid infusion is shown to cause insulin resistance in peripheral organs. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=153&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Acute lipid infusion. protocols.io dx.doi.org/10.17504/protocols.io.xm5fk86 | 2021-03-29 03:08:36 | ||
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U Mass - Lactate dehydrogenase Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.x2yfqfw | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. | University of Massachusetts | http://mmpc.org/shared/document.aspx?id=178&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Lactate dehydrogenase. protocols.io dx.doi.org/10.17504/protocols.io.x2yfqfw | 2021-03-29 03:08:33 | ||
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Vandy - Mouse Echocardiography Resource Report Resource Website |
Chee Lim | 10.17504/protocols.io.yyifxue | Mouse Metabolic Phenotyping Centers | Summary:Transthoracic mouse echocardiography is used to provide noninvasive imaging of the heart and allows for quantification of myocardialwall and chamber dimensions and systolic and diastolic performance. | Vanderbilt University | https://mmpc.org/shared/document.aspx?id=225&docType=Protocol | 1 | 2019 | Chee Lim 2019. Vandy - Mouse Echocardiography. protocols.io dx.doi.org/10.17504/protocols.io.yyifxue | 2021-03-29 03:08:37 | ||
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Case - Intraperitoneal Insulin Tolerance Test Resource Report Resource Website |
Henri Brunengraber | 10.17504/protocols.io.yefftbn | Mouse Metabolic Phenotyping Centers | This is the standard protocol for routine insulin tolerance testing. It is performed on awake mice, fasted for 18 hr (overnight) or for 6 hours. | Case Western Reserve University | https://mmpc.org/shared/document.aspx?id=272&docType=Protocol | 1 | 2019 | Henri Brunengraber 2019. Case - Intraperitoneal Insulin Tolerance Test. protocols.io dx.doi.org/10.17504/protocols.io.yefftbn | 2021-03-29 03:08:39 | ||
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Vandy - Exercise Stress Test Resource Report Resource Website |
Louise Lantier | 10.17504/protocols.io.yxcfxiw | Mouse Metabolic Phenotyping Centers | Summary: The exercise stress test is a maximal exercise test used to calculate VO2 max. Mice are run to complete exhaustion as the treadmill speed is increased every three minutes (See table 1). During the test, direct measurements of the oxygen consumption and carbon dioxide output by the mouse are made, which are then used to calculate VO2 max, VCO2, and RER. VO2 max refers to the maximum amount of oxygen that the mouse can utilize during maximal exercise. | Vanderbilt University | https://mmpc.org/shared/document.aspx?id=237&docType=Protocol | 1 | 2019 | Louise Lantier 2019. Vandy - Exercise Stress Test. protocols.io dx.doi.org/10.17504/protocols.io.yxcfxiw | 2021-03-29 03:08:40 | ||
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UC Davis - Adiposity Resource Report Resource Website |
Jon Ramsey | 10.17504/protocols.io.ybsfsne | Mouse Metabolic Phenotyping Centers | The major fat pads of mice will be dissected and weighed. This approach provides a way to determine if an intervention has an impact on specific depots of fat. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=284&docType=Protocol | 1 | 2019 | Jon Ramsey 2019. UC Davis - Adiposity. protocols.io dx.doi.org/10.17504/protocols.io.ybsfsne | 2021-03-29 03:07:49 | ||
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UC Davis - Gut Microbiome Analysis (454-10K reads) Resource Report Resource Website |
Trina Knotts | 10.17504/protocols.io.ykjfuun | Mouse Metabolic Phenotyping Centers | The work of Gordon and colleagues (i.e., Nature. 2006 Dec 21;444(7122):1027-31) has shown that obesity can result in marked shifts in the gut microbiome in mice and other models including humans. While the role of the microbiome remains to be fully elucidated, the gut microbiota can no longer be ignored as a potentially important factor when assessing metabolic phenotype. This service involves 16S gene variable region (V1-V3 or V3-V4 directed primers) sequencing by Titanium 454 (10,000 avg seq read depth) of feces, cecal, or other GI contents. Sequences can be processed through a bioinformatics pipeline (Qiime) to taxonomically classify them and to assess alpha and beta diversity of the community. In addition, Principal Components Analysis (PCA) or partial least squares- discriminant analysis (PLS-DA) can leverage variances in the relative microbial abundances to better understand how specific microbes contribute to separation by group. Correlational analyses can identify which variables of host metadata associate with specific microbes. The Core’s gut microbiome assay will employ this approach to uncover unique microbiota fingerprints in test mice. One caveat is that with fecal samples, patterns are only a surrogate for actual gut microbiota patterns, and may not exactly reflect the intestinal populations. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=287&docType=Protocol | 1 | 2019 | Trina Knotts 2019. UC Davis - Gut Microbiome Analysis (454-10K reads). protocols.io dx.doi.org/10.17504/protocols.io.ykjfuun | 2021-03-29 03:07:48 | ||
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UC Davis - High fat diet feeding Resource Report Resource Website |
Kristin Evans | 10.17504/protocols.io.xfmfjk6 | Mouse Metabolic Phenotyping Centers | SummaryA high-fat diet of varying composition and percent fat is administered to induce obesity in mice. High-fat diet induced obesity is causally associated with insulin resistance and type 2 diabetes. Diets will be provided in consultation with the investigator needs. This service applies to specialized feeding for vendor supplied animals being supplied out of the UC Davis vendor approved barrier facility. |
RRID:IMSR_JAX:000664 RRID:IMSR_CRL:27 RRID:IMSR_TAC:b6 |
University of California, Davis | https://mmpc.org/shared/document.aspx?id=266&docType=Protocol | 1 | 2019 | Kristin Evans 2019. UC Davis - High fat diet feeding. protocols.io dx.doi.org/10.17504/protocols.io.xfmfjk6 | 2021-03-29 03:08:02 | |
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U Mass - C-reactive Peptide Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.xt5fnq6 | Mouse Metabolic Phenotyping Centers | Summary:This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of C-reactive peptide reflect systemic inflammation and may be altered in obesity. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=185&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - C-reactive Peptide. protocols.io dx.doi.org/10.17504/protocols.io.xt5fnq6 | 2021-03-29 03:08:12 |
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