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http://antibodyregistry.org/AB_2687964

Comments: Vendor recommended applications: IHC-P, Western blot, ELISA
Host Organism: goat
Clonality: polyclonal
Target(s): Septin 6

Proper citation: LSBio (LifeSpan) Cat# LS-B9200, RRID:AB_2687964 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2687987

Comments: Raised against amino acids 301-600 mapping within an internal region of VPS41 of human origin. Vendor recommended applications: WB, IP, IF, IHC-P and ELISA
Host Organism: mouse
Clonality: monoclonal
Target(s): VPS41

Proper citation: Santa Cruz Biotechnology Cat# sc-377118, RRID:AB_2687987 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2692317

Comments: Image validation available for WB, ICC/IF in MDS.
Host Organism: mouse
Clonality: monoclonal
Target(s): R1

Proper citation: Santa Cruz Biotechnology Cat# sc-377415, RRID:AB_2692317 Copy   

•   Source: Antibody Registry

Discontinued

http://antibodyregistry.org/AB_2690179

Comments: Discontinued: 2020; Applications: WB (1.0 µg/mL)
Host Organism: rabbit
Clonality: polyclonal
Target(s): PK2L1

Proper citation: Thermo Fisher Scientific Cat# PA5-68680, RRID:AB_2690179 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2717248

Comments: Applications: IHC (1:10-1:500)
Host Organism: mouse
Clonality: monoclonal
Target(s): Intestinal Alkaline Phosphatase

Proper citation: Thermo Fisher Scientific Cat# MA5-24773, RRID:AB_2717248 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721197

Comments: Originally developed by Dr. Lester I. Binder at the University of Alabama---Original Report: The distribution of tau in the mammalian central nervous system. Binder LI, Frankfurter A, Rebhun LI. J Cell Biol. 1985 Oct;101(4):1371-8. PMID:3930508; PMCID: PMC2113928.Dephosphorylation dependence: Phosphorylation determines two distinct species of Tau in the central nervous system. Papasozomenos SC, Binder LI. . Cell Motil Cytoskeleton. 1987;8(3):210-26. PMID: 2446784.; Neurofibrillary tangles of Alzheimer disease share antigenic determinants with the axonal microtubule-associated protein tau. Wood JG, Mirra SS, Pollock NJ, Binder LI. Proc Natl Acad Sci U S A. 1986 Jun;83(11):4040-3. PMID: 2424015, PMCID: PMC323661.; Abnormal phosphorylation of the microtubule-associated protein tau in Alzheimer cytoskeletal pathologyGrundke-Iqbal I, Iqbal K, Tung YC, Quinlan M, Wisniewski HM, Binder LI. Proc Natl Acad Sci U S A. 1986. 83(13):4913-7. PMID: 3088567, PMCID: PMC323854.Epitope: Application of synthetic phospho- and unphospho- peptides to identify phosphorylation sites in a subregion of the tau molecule, which is modified in Alzheimer's disease.Liu WK, Moore WT, Williams RT, Hall FL, Yen SH. J Neurosci Res. 1993 Feb 15;34(3):371-6. PMID: 8455212; The structural basis of monoclonal antibody Alz50's selectivity for Alzheimer's disease pathologyCarmel G, Mager EM, Binder LI, Kuret J. . J Biol Chem. 1996. 271(51):32789-95. PMID: 8955115. Epitopes that span the tau molecule are shared with paired helical filamentsKosik KS, Orecchio LD, Binder L, Trojanowski JQ, Lee VM, Lee G. . Neuron. 1988. 1(9):817-25. PMID: 2483104.Monoclonal Antibody Selection and Production: BALB/c mice were immunized with phosphocellulose-purified (51) bovine MAPs, which were denatured by boiling in 0.1% SDS. Immunization was performed over a period of 2 me, with the initial injection performed in Freund's complete adjuvant and subsequent injections (at 2-wk intervals) carried out using Freund's incomplete adjuvant. Each injection contained at least 0.5 mg SDSdenatured bovine MAPs administered at both an interperitoneal and a subcutaneous site. 5 d before fusion, a final injection was administered to two animals. Immunized spleens were dissected and dissociated, and the splenocytes were fused with SP2/o myeloma cells by the procedure of Kohler and Milstein (25, 26) as modified (49). The resultant hybrid cells were plated in 12 96-well culture dishes on a thymocyte feeder layer in RPMI medium supplemented with 15% horse serum, and 24 h after plating this medium was exchanged with selection medium containing hypoxanthine, aminopterin, thymidine. Clone formation was observed within 7 d and most wells were confluent 14 d after fusion. Of the 1,156 wells plated, 1,155 were positive for hybrid formation. Antibody production was assayed by enzyme-linked immunosorbant assay (ELISA) (50) (see below, Quantitative ELISA) using phosphocellulose MAPs in the solid phase. Of the 1,155 wells assayed, >800 were positive for anti-MAP antibody production. 156 of the most intensely positive clones were picked and expanded into l-ml cultures. Upon reaching confluence, the individual clones were frozen in complete medium that contained 25% horse serum and 10% dimethyl sulfoxide. Aliquots of spent medium from each clone were tested by ELISA for binding to purified bovine MAP2 or tau. Of the 156 clones frozen, 31 expressed antibody to MAP2 whereas only 6 produced antibody that recognized bovine tau. The antibody-containing media from these six putative tau clones were tested on immunoblots of whole rat brain. Only one line, Taul, was cross-reactive in rat brain, exhibiting binding to several polypeptides in the tau region of the gel. This line was thawed and subcloned twice before injection into a BALB/c mouse for the production of antibody-containing ascites fluid. Cells obtained from sterilely drained ascites fluid were harvested by centrifugation, cultured, and subcloned once more before freezing. I 1 ml ascites fluid was harvested from one mouse and chromatographed on protein A-Sepharose, yielding nearly 30 mg of Tau-l antibody at a final concentration of 0.96 mg/ml. Except for fluctuation analysis, this affinity-purified antibody was used in all of the biochemical experiments described below. Immunocytochemistry was performed using spent tissue culture medium harvested from stationary phase Tau-l-producing hybridomas.
Host Organism: mouse
Clonality: monoclonal
Target(s): Tau protein

Proper citation: Nicholas M. Kanaan at Michigan State University Cat# Tau1, RRID:AB_2721197 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2728756

Comments: Applications: W, IP, IF-IC
Host Organism: rabbit
Clonality: monoclonal
Target(s): eNOS

Proper citation: Cell Signaling Technology Cat# 32027, RRID:AB_2728756 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2718851

Comments: Discontinued: 2023; Applications: WB (1:2500)
Host Organism: rabbit
Clonality: polyclonal
Target(s): PTK7

Proper citation: Thermo Fisher Scientific Cat# PA5-72997, RRID:AB_2718851 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2631231

Comments: Applications: Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Host Organism: Mouse
Clonality: monoclonal
Target(s): GFAP

Proper citation: Novus Cat# NBP2-29415, RRID:AB_2631231 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_3309150

Comments: Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Host Organism: Mouse
Clonality: monoclonal
Target(s): Cytokeratin 8

Proper citation: Novus Cat# NBP2-44931, RRID:AB_3309150 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_3283640

Comments: Applications: Immunohistochemistry, Immunohistochemistry-Paraffin
Host Organism: Mouse
Clonality: monoclonal
Target(s): TAG-72

Proper citation: Novus Cat# NBP2-33128AF532, RRID:AB_3283640 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_3338353

Comments: Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, CyTOF-ready
Host Organism: Mouse
Clonality: monoclonal
Target(s): TIMP-2

Proper citation: Novus Cat# NBP2-54555B, RRID:AB_3338353 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_3533861

Comments: Applications: Western Blot, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence
Host Organism: Mouse
Clonality: monoclonal
Target(s): PEA-15

Proper citation: Novus Cat# NBP3-05544IR, RRID:AB_3533861 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_3321050

Comments: Applications: Western Blot, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating
Host Organism: Mouse
Clonality: monoclonal
Target(s): Calbindin D-28K

Proper citation: Novus Cat# NBP2-50048AF750, RRID:AB_3321050 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_3338342

Comments: Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular), CyTOF-ready
Host Organism: Mouse
Clonality: monoclonal
Target(s): TIMP-2

Proper citation: Novus Cat# NBP2-54554V, RRID:AB_3338342 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_11101532

Comments: manufacturer recommendations: IgG Flow-Cyt(1:50-200), IF(1:50-200)
Clonality: polyclonal
Target(s): Rabbit LTB4-R2 Cy7

Proper citation: Bioss Cat# bs-2655R-Cy7, RRID:AB_11101532 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_3560167

Comments: Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Host Organism: Rabbit
Clonality: monoclonal
Target(s): Cytokeratin, pan

Proper citation: Novus Cat# NBP3-08398H, RRID:AB_3560167 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_3581326

Comments: Applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence
Host Organism: Mouse
Clonality: monoclonal
Target(s): Myosin heavy chain 11

Proper citation: Novus Cat# NBP3-11585JF646, RRID:AB_3581326 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_904921

Comments: vendor suggested use: IgG; IgG Immunocytochemistry; Western Blot; ICC, WB
Host Organism: mouse
Clonality: monoclonal
Target(s): PRPH / Peripherin

Proper citation: LSBio (LifeSpan) Cat# LS-C26026-300, RRID:AB_904921 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_1006820

Comments: manufacturer recommendations: ELISA; Immunoprecipitation; Western Blot; Enzyme Immunoassay, Immunoprecipitation, Western Blot
Host Organism: goat
Clonality: polyclonal
Target(s): Pyruvate Kinase

Proper citation: Acris Antibodies Cat# R1108P, RRID:AB_1006820 Copy   

•   Source: Antibody Registry