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http://antibodyregistry.org/AB_2721017

Comments: Applications: Isotype control, Flow cytometry
Host Organism: mouse
Clonality: monoclonal
Target(s): KLH

Proper citation: BD Biosciences Cat# 564416, RRID:AB_2721017 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721204

Comments: Applications: WB, IP and ELISA
Host Organism: mouse
Clonality: monoclonal
Target(s): FOXP2

Proper citation: Santa Cruz Biotechnology Cat# sc-517261, RRID:AB_2721204 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721186

Comments: recommended for detection of TEF-1 by WB, IP, IF, IHC(P) and ELISA
Host Organism: mouse
Clonality: monoclonal
Target(s): TEF-1

Proper citation: Santa Cruz Biotechnology Cat# sc-393976, RRID:AB_2721186 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721194

Comments: Original Report: Functional implications for the microtubule-associated protein tau: localization in oligodendrocytes. LoPresti P, Szuchet S, Papasozomenos SC, Zinkowski RP, Binder LI. Proc Natl Acad Sci U S A. 1995 Oct 24;92(22):10369-73. PMID: 7479786; PMCID: PMC40798. _x000D_\n_x000D_\nEpitope: The structural basis of monoclonal antibody Alz50's selectivity for Alzheimer's disease pathology. Carmel G, Mager EM, Binder LI, Kuret J. J Biol Chem. 1996 Dec 20;271(51):32789-95. PMID: 8955115_x000D_\n_x000D_\nUpdated Epitope: Porzig, R., Singer, D., and Hoffmann, R. (2007). Epitope mapping of mAbs AT8 and Tau5 directed against hyperphosphorylated regions of the human tau protein. Biochem. Biophys. Res. Commun. 358, 644–649. doi: 10.1016/j.bbrc.2007.04.187
Host Organism: mouse
Clonality: monoclonal
Target(s): Tau protein

Proper citation: Nicholas M. Kanaan at Michigan State University Cat# Tau5, RRID:AB_2721194 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721215

Host Organism: mouse
Clonality: monoclonal
Target(s): Angiotensinogen

Proper citation: Swant Cat# 138, RRID:AB_2721215 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721197

Comments: Originally developed by Dr. Lester I. Binder at the University of Alabama---Original Report: The distribution of tau in the mammalian central nervous system. Binder LI, Frankfurter A, Rebhun LI. J Cell Biol. 1985 Oct;101(4):1371-8. PMID:3930508; PMCID: PMC2113928.Dephosphorylation dependence: Phosphorylation determines two distinct species of Tau in the central nervous system. Papasozomenos SC, Binder LI. . Cell Motil Cytoskeleton. 1987;8(3):210-26. PMID: 2446784.; Neurofibrillary tangles of Alzheimer disease share antigenic determinants with the axonal microtubule-associated protein tau. Wood JG, Mirra SS, Pollock NJ, Binder LI. Proc Natl Acad Sci U S A. 1986 Jun;83(11):4040-3. PMID: 2424015, PMCID: PMC323661.; Abnormal phosphorylation of the microtubule-associated protein tau in Alzheimer cytoskeletal pathologyGrundke-Iqbal I, Iqbal K, Tung YC, Quinlan M, Wisniewski HM, Binder LI. Proc Natl Acad Sci U S A. 1986. 83(13):4913-7. PMID: 3088567, PMCID: PMC323854.Epitope: Application of synthetic phospho- and unphospho- peptides to identify phosphorylation sites in a subregion of the tau molecule, which is modified in Alzheimer's disease.Liu WK, Moore WT, Williams RT, Hall FL, Yen SH. J Neurosci Res. 1993 Feb 15;34(3):371-6. PMID: 8455212; The structural basis of monoclonal antibody Alz50's selectivity for Alzheimer's disease pathologyCarmel G, Mager EM, Binder LI, Kuret J. . J Biol Chem. 1996. 271(51):32789-95. PMID: 8955115. Epitopes that span the tau molecule are shared with paired helical filamentsKosik KS, Orecchio LD, Binder L, Trojanowski JQ, Lee VM, Lee G. . Neuron. 1988. 1(9):817-25. PMID: 2483104.Monoclonal Antibody Selection and Production: BALB/c mice were immunized with phosphocellulose-purified (51) bovine MAPs, which were denatured by boiling in 0.1% SDS. Immunization was performed over a period of 2 me, with the initial injection performed in Freund's complete adjuvant and subsequent injections (at 2-wk intervals) carried out using Freund's incomplete adjuvant. Each injection contained at least 0.5 mg SDSdenatured bovine MAPs administered at both an interperitoneal and a subcutaneous site. 5 d before fusion, a final injection was administered to two animals. Immunized spleens were dissected and dissociated, and the splenocytes were fused with SP2/o myeloma cells by the procedure of Kohler and Milstein (25, 26) as modified (49). The resultant hybrid cells were plated in 12 96-well culture dishes on a thymocyte feeder layer in RPMI medium supplemented with 15% horse serum, and 24 h after plating this medium was exchanged with selection medium containing hypoxanthine, aminopterin, thymidine. Clone formation was observed within 7 d and most wells were confluent 14 d after fusion. Of the 1,156 wells plated, 1,155 were positive for hybrid formation. Antibody production was assayed by enzyme-linked immunosorbant assay (ELISA) (50) (see below, Quantitative ELISA) using phosphocellulose MAPs in the solid phase. Of the 1,155 wells assayed, >800 were positive for anti-MAP antibody production. 156 of the most intensely positive clones were picked and expanded into l-ml cultures. Upon reaching confluence, the individual clones were frozen in complete medium that contained 25% horse serum and 10% dimethyl sulfoxide. Aliquots of spent medium from each clone were tested by ELISA for binding to purified bovine MAP2 or tau. Of the 156 clones frozen, 31 expressed antibody to MAP2 whereas only 6 produced antibody that recognized bovine tau. The antibody-containing media from these six putative tau clones were tested on immunoblots of whole rat brain. Only one line, Taul, was cross-reactive in rat brain, exhibiting binding to several polypeptides in the tau region of the gel. This line was thawed and subcloned twice before injection into a BALB/c mouse for the production of antibody-containing ascites fluid. Cells obtained from sterilely drained ascites fluid were harvested by centrifugation, cultured, and subcloned once more before freezing. I 1 ml ascites fluid was harvested from one mouse and chromatographed on protein A-Sepharose, yielding nearly 30 mg of Tau-l antibody at a final concentration of 0.96 mg/ml. Except for fluctuation analysis, this affinity-purified antibody was used in all of the biochemical experiments described below. Immunocytochemistry was performed using spent tissue culture medium harvested from stationary phase Tau-l-producing hybridomas.
Host Organism: mouse
Clonality: monoclonal
Target(s): Tau protein

Proper citation: Nicholas M. Kanaan at Michigan State University Cat# Tau1, RRID:AB_2721197 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721080

Comments: Applications: IHC,IHC-P
Host Organism: mouse
Clonality: monoclonal
Target(s): Tau

Proper citation: Synaptic Systems Cat# 314 111, RRID:AB_2721080 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721090

Comments: Applications: WB,IP,ICC,IHC,IHC-P,ELISA
Host Organism: mouse
Clonality: monoclonal
Target(s): GFAP

Proper citation: Synaptic Systems Cat# 173 211, RRID:AB_2721090 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721193

Comments: Originally created by Dr. Lester I. Binder at Northwestern University---Original Report: Conformational changes and truncation of tau protein during tangle evolution in Alzheimer's disease. García-Sierra F, Ghoshal N, Quinn B, Berry RW, Binder LI. J Alzheimers Dis. 2003 Apr;5(2):65-77. PMID: 12719624Epitope: Pathological conformations involving the amino terminus of tau occur early in Alzheimer's disease and are differentially detected by monoclonal antibodies. Combs B, Hamel C, Kanaan NM. Neurobiol Dis. 2016 Oct;94:18-31. doi: 10.1016/j.nbd.2016.05.016. Epub 2016 May 31. PMID: 27260838
Host Organism: mouse
Clonality: monoclonal
Target(s): tau

Proper citation: Nicholas M. Kanaan at Michigan State University Cat# Tau13, RRID:AB_2721193 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721211

Host Organism: mouse
Clonality: monoclonal
Target(s): IGF1

Proper citation: Swant Cat# 41, RRID:AB_2721211 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721190

Comments: NIH Aids Reagent Program catalog number 12156, Lot number 110220
Li, M.M., Wu, L.I., and Emerman, M. (2010). The range of human APOBEC3H sensitivity to lentiviral Vif proteins. J. Virol. 84, 88–95. PMCID: PMC2798431
Host Organism: mouse
Clonality: monoclonal
Target(s): Human APOBEC3H

Proper citation: NIH HIV Reagent Program Cat# 12156, RRID:AB_2721190 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721031

Comments: Application: Cell Isolation; ELISA; Flow Cytometry; Immunocytochemistry; Immunofluorescence; Immunohistochemistry
Host Organism: mouse
Clonality: monoclonal
Target(s): SSEA-4

Proper citation: STEMCELL Technologies Cat# 60062, RRID:AB_2721031 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721039

Comments: Applications: WB, IHC, IP
Campbell, A. J., et al. (2010). Br J Haematol. 149(2):221-230
Host Organism: mouse
Clonality: monoclonal
Target(s): FOXP2

Proper citation: Millipore Cat# MABE415, RRID:AB_2721039 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721195

Comments: Originally developed by Dr. Lester I. Binder at Northwestern University---N-terminal fragments of tau inhibit full-length tau polymerization in vitro. Horowitz PM, LaPointe N, Guillozet-Bongaarts AL, Berry RW, Binder LI. Biochemistry. 2006 Oct 24;45(42):12859-66. PMID: 17042504
Host Organism: mouse
Clonality: monoclonal
Target(s): Tau protein

Proper citation: Nicholas M. Kanaan at Michigan State University Cat# Tau7, RRID:AB_2721195 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721015

Comments: Flow cytometry
Host Organism: mouse
Clonality: monoclonal
Target(s): Human CD42b

Proper citation: BD Biosciences Cat# 565427, RRID:AB_2721015 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721237

Comments: Yanamandra et al., 2013 "HJ9.3 and HJ9.4 mouse monoclonal antibodies were raised by immunizing tau knockout mice (The Jackson laboratory) against mouse tau and HJ8.5 and HJ8.7 monoclonal antibodies were raised by immunizing tau knockout mice against human tau."
Host Organism: mouse
Clonality: monoclonal
Target(s): tau

Proper citation: D. Holtzman at Washington University School of Medicine; Missouri; USA Cat# HJ8.5, RRID:AB_2721237 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721213

Host Organism: mouse
Clonality: monoclonal
Target(s): IGF2

Proper citation: Swant Cat# 79, RRID:AB_2721213 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721209

Host Organism: mouse
Clonality: monoclonal
Target(s): Glutamate

Proper citation: Swant Cat# 2D7, RRID:AB_2721209 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2721251

Comments: Applications: WB, IP, IF and ELISA
Host Organism: mouse
Clonality: monoclonal
Target(s): DDX21

Proper citation: Santa Cruz Biotechnology Cat# sc-376758, RRID:AB_2721251 Copy   

•   Source: Antibody Registry

http://antibodyregistry.org/AB_2727929

Comments: Info: This product is a higher concentration for optimized use in multicolor flow cytometry panels. It replaces product cat # 130-096-164. (RRID:AB_10828693).
Host Organism: mouse
Clonality: monoclonal
Target(s): CD279 (PD1)

Proper citation: Miltenyi Biotec Cat# 130-117-384, RRID:AB_2727929 Copy   

•   Source: Antibody Registry