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Cellosaurus is database indexing available cell lines from various collections: American Type Culture Collection (ATCC), Bioresource Collection and Research Center, Taiwan (BCRC), Banco de Células do Rio de Janeiro (BCRJ), CellBank Australia (CBA), Collection of Cell Lines in Veterinary Medicine (CCLV), Cell Lines Service (CLS), Coriell, Drosophila Genomics Resource Center (DGRC), Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ), European Bank for induced pluripotent Stem Cells (EBiSC), European Collection of Authenticated Cell Cultures (ECACC), Interlab Cell Line Collection (ICLC), Institute for Fermentation Osaka (IFO), Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER) biobank, Japanese Collection of Research Bioresources (JCRB) Cell Bank, Kunming Cell Bank of Type Culture Collection (KCB), Korean Cell Line Bank (KCLB), Millipore, National Cell Bank of Iran (NCBI_Iran), National Cancer Institute - Developmental Therapeutics Program (NCI-DTP), NINDS Human Cell and Data Repository (NHCDR), NIH AIDS Reagent Program (NIH-ARP), NISES, RIKEN Bioresource Center Cell Bank (RCB), Royan Stem Cell Bank (RSCB), Tick Cell Biobank (TCB), Tohoku University cell line catalog (TKG), and Ximbio.
Contaminated
https://web.expasy.org/cellosaurus/CVCL_4380
Organism: Homo sapiens (Human)
Disease: Diffuse large B-cell lymphoma
Category: Cancer cell line
Comments: Caution: Was reported in Cellosaurus and the ICLAC Register (from 2018 to early 2022) to be a SU-DHL-8 contaminated cell line but there seem to have been an issue with the sample tested. DSMZ has obtained original stock from Epstein A.L. and SU-DHL-7 is a genuine non-problematic cell line.
Proper citation: DSMZ Cat# ACC-903, RRID:CVCL_4380 Copy
Contaminated
https://web.expasy.org/cellosaurus/CVCL_4380
Organism: Homo sapiens (Human)
Disease: Diffuse large B-cell lymphoma
Category: Cancer cell line
Comments: Caution: Was reported in Cellosaurus and the ICLAC Register (from 2018 to early 2022) to be a SU-DHL-8 contaminated cell line but there seem to have been an issue with the sample tested. DSMZ has obtained original stock from Epstein A.L. and SU-DHL-7 is a genuine non-problematic cell line.
Proper citation: RRID:CVCL_4380 Copy
https://web.expasy.org/cellosaurus/CVCL_GP63
Organism: Homo sapiens (Human)
Disease: Glioblastoma
Category: Cancer cell line
Comments: Caution: This is the original U-87MG cell line established in 1968 at the University of Uppsala. The cell line distributed by most cell collections (including ATCC, CLS, ECACC, etc.) is a different cell line whose origin is not yet clear (PubMed=27582061). See U-87MG ATCC (Cellosaurus=CVCL_0022) for the cell line used by most research groups.
Proper citation: RRID:CVCL_GP63 Copy
https://web.expasy.org/cellosaurus/CVCL_D6MH
Organism: Homo sapiens (Human)
Category: Induced pluripotent stem cell
Comments: Caution: There is a major issue with this cell line. It is indicated to be derived from ATCC-DYR0100 (Cellosaurus=CVCL_C253), a male cell line, but the karyotype of this cell line is female and the paper also indicates that it originate from a Chinese female newborn., Population: Chinese., From: Sun Yat-sen University First Affiliated Hospital; Guangzhou; China.
Proper citation: RRID:CVCL_D6MH Copy
https://web.expasy.org/cellosaurus/CVCL_E3E9
Organism: Homo sapiens (Human)
Disease: Ovarian serous cystadenocarcinoma
Category: Cancer cell line
Comments: Characteristics: Transfected with the pVgRXR vector that contains the VgEcR fusion protein under the control of a CMV promoter and RXRA under the control of a RSV promoter as well as BleoR. Also transfected with an empty pIND vector (PubMed=15755800)., Characteristics: Control cell line for SK-OV-3/pVgRXR/pINDp53wt (Cellosaurus=CVCL_E3EA) (PubMed=15755800)., Population: Caucasian.
Proper citation: RRID:CVCL_E3E9 Copy
https://web.expasy.org/cellosaurus/CVCL_G366
Organism: Homo sapiens (Human)
Disease: Renal cell carcinoma
Category: Cancer cell line
Comments: Caution: The age/sex of donor of this cell line and that of NKK1 (Cellosaurus=CVCL_G365), both described in PubMed=9183637 are inverted in the abstract of that paper. We believe that the information in the material and methods section is the correct one as it is corroborated by the karyotype information in the same paper., Population: Japanese.
Proper citation: RRID:CVCL_G366 Copy
https://web.expasy.org/cellosaurus/CVCL_G366
Organism: Homo sapiens (Human)
Disease: Renal cell carcinoma
Category: Cancer cell line
Comments: Caution: The age/sex of donor of this cell line and that of NKK1 (Cellosaurus=CVCL_G365), both described in PubMed=9183637 are inverted in the abstract of that paper. We believe that the information in the material and methods section is the correct one as it is corroborated by the karyotype information in the same paper., Population: Japanese.
Proper citation: TKG Cat# TKG 0700, RRID:CVCL_G366 Copy
https://web.expasy.org/cellosaurus/CVCL_E8DB
Organism: Homo sapiens (Human)
Category: Transformed cell line
Comments: Characteristics: SEAP (secreted ALPP) reporter cell line, in this cell line SEAP is under the control of the ISG54 promoter (InvivoGen=hkb-ifnabv2)., Characteristics: Designed to detect bioactive human type I IFNs by monitoring the activation of the ISGF3 pathway. Has an improved Type I IFN response compared to HEK-Blue IFN-alpha/beta (Cellosaurus=CVCL_KT26) (InvivoGen=hkb-ifnabv2).
Proper citation: RRID:CVCL_E8DB Copy
https://web.expasy.org/cellosaurus/CVCL_E8DB
Organism: Homo sapiens (Human)
Category: Transformed cell line
Comments: Characteristics: SEAP (secreted ALPP) reporter cell line, in this cell line SEAP is under the control of the ISG54 promoter (InvivoGen=hkb-ifnabv2)., Characteristics: Designed to detect bioactive human type I IFNs by monitoring the activation of the ISGF3 pathway. Has an improved Type I IFN response compared to HEK-Blue IFN-alpha/beta (Cellosaurus=CVCL_KT26) (InvivoGen=hkb-ifnabv2).
Proper citation: RRID:CVCL_E8DB Copy
Contaminated
https://web.expasy.org/cellosaurus/CVCL_M629
Organism: Homo sapiens (Human)
Disease: Human papillomavirus-related endocervical adenocarcinoma
Category: Cancer cell line
Comments: Population: African American., Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=6451928; PubMed=20143388). Originally thought to originate from a male patient with a lung adenocarcinoma. The 2563 cell line is said to be derived from the MAC-21 cell line (Cellosaurus=CVCL_M627) which is also HeLa contaminated but which has never been tested for such a contamination..
Proper citation: RRID:CVCL_M629 Copy
https://web.expasy.org/cellosaurus/CVCL_HF20
Organism: Homo sapiens (Human)
Category: Transformed cell line
Comments: Characteristics: This cell line is transfected with EBNA1t, a truncated version of EBV EBNA1 lacking the Gly-Gly-Ala repeats region. It has an enhanced ability to produce recombinant protein compared to HEK293-EBNA (Cellosaurus=CVCL_6974)., Characteristics: The use of recombinant protein expression vectors containing the EBV oriP in cell lines stably expressing EBV's EBNA1 protein significantly increases recombinant protein yield., Group: Patented cell line.
Proper citation: RRID:CVCL_HF20 Copy
https://web.expasy.org/cellosaurus/CVCL_3317
Organism: Homo sapiens (Human)
Disease: Melanoma
Category: Cancer cell line
Comments: Caution: The exact relationship between HMCB (Cellosaurus=CVCL_3317) and RPMI-7262/7272/7932/7962/7972 is not clear: all are derived from patient 'Bowes' melanoma., Anecdotal: The Bowes cell line is famous for its use in the identification and purification of tissue-type plasminogen activator, tPA., Population: Caucasian., Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE)., Group: Patented cell line.
Proper citation: RRID:CVCL_3317 Copy
Possibly Discontinued Discontinued
https://web.expasy.org/cellosaurus/CVCL_0580
Organism: Homo sapiens (Human)
Disease: Cutaneous melanoma
Category: Cancer cell line
Comments: Caution: The reported STR profile from Wistar of this cell line was changed at one point between February 2016 when we retrieved them and entered them in Cellosaurus and May 2018. The major changes were: D18S51., Population: Caucasian., Part of: Wistar Institute melanoma cell line collection., Part of: COSMIC cell lines project., Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Proper citation: RRID:CVCL_0580 Copy
Misidentified/Contaminated
https://web.expasy.org/cellosaurus/CVCL_1402
Organism: Homo sapiens (Human)
Disease: Melanoma
Category: Cancer cell line
Comments: Population: Caucasian., Part of: COSMIC cell lines project., Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE)., Problematic cell line: Misidentified/contaminated. Shown to be identical to MEL-Wei. This cell line was reported to be isogenic with LCL-Ho (Cellosaurus=CVCL_1868) but it does not have the same STR profile (PubMed=10508494; PubMed=20143388). According to DSMZ ACC-62 it is a human female cell line..
Proper citation: RRID:CVCL_1402 Copy
Partially Misidentified
https://web.expasy.org/cellosaurus/CVCL_5594
Organism: Homo sapiens (Human)
Disease: Breast carcinoma
Category: Cancer cell line
Comments: Part of: JWGray breast cancer cell line panel., Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE)., Problematic cell line: Partially misidentified. For a period of time Asterand (now BioIVT) mistakenly distributed SUM149PT (Cellosaurus=CVCL_3422) under the designation of SUM229PE. This leads to the publication of a STR profile (PubMed=25877200) which was almost identical to that of SUM149PT.., Group: Triple negative breast cancer (TNBC) cell line.
Proper citation: RRID:CVCL_5594 Copy
https://web.expasy.org/cellosaurus/CVCL_VQ57
Organism: Homo sapiens (Human)
Disease: Childhood B acute lymphoblastic leukemia
Category: Cancer cell line
Comments: Caution: Was originally (PubMed=23545498) described as originating from the Seraphina cell line (Cellosaurus=CVCL_M646) but was later (PubMed=28494875) shown to derive from RCH-ACV., Characteristics: Transduced with the retrovirus vector C/EBPaER-GFP which consist of CEBPA fused with the estrogen receptor hormone binding domain and GFP., Characteristics: Can efficiently be convert into cells exhibiting increased adherent, phagocytic and quiescent properties with a transcriptome resembling normal macrophages., Population: Caucasian.
Proper citation: RRID:CVCL_VQ57 Copy
https://web.expasy.org/cellosaurus/CVCL_0303
Organism: Mus musculus (Mouse)
Category: Transformed cell line
Comments: Caution: The STR profile of HL-1 as distributed from Ubigene shows that the cell line that what they distribute is not HL-1 but C2C12 (Cellosaurus=CVCL_0188) or a derivative of that cell line (Ubigene=YC-C100)., Characteristics: From transgenic mice expressing a fusion gene comprised of the atrial natriuretic factor promoter and the SV40 early region., Characteristics: Spontaneously contract while maintaining a differentiated cardiac phenotype (Millipore=SCC065)., Group: Patented cell line.
Proper citation: RRID:CVCL_0303 Copy
https://web.expasy.org/cellosaurus/CVCL_0303
Organism: Mus musculus (Mouse)
Category: Transformed cell line
Comments: Caution: The STR profile of HL-1 as distributed from Ubigene shows that the cell line that what they distribute is not HL-1 but C2C12 (Cellosaurus=CVCL_0188) or a derivative of that cell line (Ubigene=YC-C100)., Characteristics: From transgenic mice expressing a fusion gene comprised of the atrial natriuretic factor promoter and the SV40 early region., Characteristics: Spontaneously contract while maintaining a differentiated cardiac phenotype (Millipore=SCC065)., Group: Patented cell line.
Proper citation: Millipore Cat# SCC065, RRID:CVCL_0303 Copy
https://web.expasy.org/cellosaurus/CVCL_0303
Organism: Mus musculus (Mouse)
Category: Transformed cell line
Comments: Caution: The STR profile of HL-1 as distributed from Ubigene shows that the cell line that what they distribute is not HL-1 but C2C12 (Cellosaurus=CVCL_0188) or a derivative of that cell line (Ubigene=YC-C100)., Characteristics: From transgenic mice expressing a fusion gene comprised of the atrial natriuretic factor promoter and the SV40 early region., Characteristics: Spontaneously contract while maintaining a differentiated cardiac phenotype (Millipore=SCC065)., Group: Patented cell line.
Proper citation: Ubigene Cat# YC-C100, RRID:CVCL_0303 Copy
https://web.expasy.org/cellosaurus/CVCL_RU08
Organism: Homo sapiens (Human)
Category: Transformed cell line
Comments: Caution: Could be identical to 293T R-Spondin1 expressing (Cellosaurus=CVCL_RU09) distributed by Millipore. Both cell line product pages cite PubMed=19398967 but the Millipore page states "Cell line is not the published cell line from the Kuo lab, but rather a related cell line developed in the same lab using the reference protocol"., Characteristics: Expresses mouse Fc-Rspo1 which consist of the fusion of mouse IgG2a Fc region to the N-terminal of Rspo1 with a N-terminal HA epitope tag (AMSBIO).
Proper citation: RRID:CVCL_RU08 Copy
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