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Anna Martinez Casals, Cecilia Lindskog 2019. Standardized immunohistochemical staining used in the Human Protein Atlas. protocols.io dx.doi.org/10.17504/protocols.io.yj8furwCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.yj8furw
Authors: Anna Martinez Casals, Cecilia Lindskog
Group: Human Protein Atlas
Summary: The Human Protein Atlas provides a map showing the distribution and relative abundance of proteins in the human body. All IHC staining in the Human Protein Atlas project are performed using the following standard protocol. The primary antibody dilution is based on titration optimization, the dilution suggested by the Human Protein Atlas can be found under antibody and antigen information for each antibody. When primary antibody originates from other host animals than rabbit, there are some modifications and different secondary antibody is used.
Affiliations: Science for Life Lab / Science for life laboratory / KTH - Royal Institute of Technology, Science for Life Lab / Science for life laboratory / KTH - Royal Institute of Technology
External URL: https://www.ncbi.nlm.nih.gov/pubmed/22688270
Version: 1
Publication Date: 2019
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Source: Protocols.io
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