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Takao Ito, Ryoji Nakaune 2017. A protocol of molecular detection of phytoplasmas and Xylella spp. in post-entry quarantine for plants.. protocols.io dx.doi.org/10.17504/protocols.io.jhjcj4nCopy Citation Copied
URL: https://dx.doi.org/10.17504/protocols.io.jhjcj4n
Authors: Takao Ito, Ryoji Nakaune
Summary: In the STEPS, we describe TaqMan multiplex real-time PCR to universally detect phytoplasmas (PP) and Xylella spp. (XL) with plant internal control (IC) from crude extracts. A protocol file uploaded in the DESCRIPTION shows further details of the protocol in Japanese and English.
Associated Publications: Ito T, Suzaki K (2017) Universal detection of phytoplasmas and Xylella spp. by TaqMan singleplex and multiplex real-time PCR with dual priming oligonucleotides. PLoS ONE 12(9): e0185427. doi: 10.1371/journal.pone.0185427
Affiliations: Division of Grape and Persimmon Research, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), Higashihiroshima, Hiroshima, Japan,
External URL: https://doi.org/10.1371/journal.pone.0185427
Version: 1
Publication Date: 2017
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Source: Protocols.io
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