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Protocol Name
Script P6: HPyV Detection
DOI:10.17504/protocols.io.efxbbpn RRID Copied  
PDF Report How to cite
HANNIGAN GD,  GRICE EA,  ET AL. 2016. Script P6: HPyV Detection. protocols.io dx.doi.org/10.17504/protocols.io.efxbbpn
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Protocol Information

URL: https://dx.doi.org/10.17504/protocols.io.efxbbpn

Authors: HANNIGAN GD, GRICE EA, ET AL.

Group: VERVE Net, Club Grice

Summary: This protocol provides a method for detecting Human Polyomaviruses (HPyVs) in our virome and whole metagenome datasets. For genome reference, download the HPyV genomes (in fasta format) from NCBI (nucleotide) using the search terms "Human Polyomavirus" AND "Complete Genome". This is used in the rest of the analyses. Based on the methods from the following publication:Hannigan, Geoffrey D., et al. "The Human Skin Double-Stranded DNA Virome: Topographical and Temporal Diversity, Genetic Enrichment, and Dynamic Associations with the Host Microbiome." mBio 6.5 (2015): e01578-15.

Associated Publications: Kindler L, Stoliartchouk A, Teytelman L, Hurwitz BL, Method-centered digital communities on protocols.io for fast-paced scientific innovation. F1000Research doi: 10.12688/f1000research.9453.2

Affiliations: Grice Lab, DEPARTMENT OF DERMATOLOGY UNIVERSITY OF PENNSYLVANIA, Grice Lab, DEPARTMENT OF DERMATOLOGY UNIVERSITY OF PENNSYLVANIA, Grice Lab, DEPARTMENT OF DERMATOLOGY UNIVERSITY OF PENNSYLVANIA

External URL: http://mbio.asm.org/content/6/5/e01578-15.full

Version: 1

Publication Date: 2016

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Source: Protocols.io