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URL: https://web.expasy.org/cellosaurus/CVCL_UP52
Proper Citation: RCB Cat# RCB4696, RRID:CVCL_UP52
Description: Cell line MEF GFP-LC3-RFP-LC3deltaG is a Spontaneously immortalized cell line with a species of origin Mus musculus (Mouse)
Sex: Sex unspecified
Defining Citation: PMID:27818143
Comments: Characteristics: Stably expresses EGFP-LC3-mRFP-LC3delta, an autophagic flux probe. GFP-LC3 is fused to the N-terminus of RFP-LC3 whose C-terminal glycine is deleted. When expressed, the probe it is separated into EGFP-LC3 and mRFP-LC3deltaG by the action of endogenous ATG4 family proteases that cleave the peptide bond after the C-terminal glycine of LC3 in the cytosol producing equimolar amounts of EGFP-LC3 and mRFP-LC3deltaG (PubMed=27818143).
Category: Spontaneously immortalized cell line
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Source: Cellosaurus
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