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URL: https://web.expasy.org/cellosaurus/CVCL_HC50
Proper Citation: Kerafast Cat# ENH103-FP, RRID:CVCL_HC50
Description: Cell line NIH 3T3 Duo Parental is a Spontaneously immortalized cell line with a species of origin Mus musculus (Mouse)
Sex: Male
Defining Citation: PMID:23929981, PMID:25255091
Comments: Characteristics: Integrates one copy of a LacO-ISceI construct (256 copies of LacO) and two copies of a TetO-ISceI-TetO construct (96 copies of TetO)., Characteristics: Cell developed for the induction and visualization of double-strand breaks chromosomal damage at specific sites of the mammalian genome. Sytem based on the stable integration of endonuclease ISceI recognition sites flanked by LacO/TetO operator arrays, coupled with retroviral-mediated integration of their fluorescent repressors (LacR/TetR) to visualize the LacO/TetO sites.
Category: Spontaneously immortalized cell line
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Source: Cellosaurus
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