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Cell Line Name

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BY-2
RRID:CVCL_D5GM RRID Copied  
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DSMZ Cat# PC-1181, RRID:CVCL_D5GM
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Cell Line Information

URL: https://web.expasy.org/cellosaurus/CVCL_D5GM

Proper Citation: DSMZ Cat# PC-1181, RRID:CVCL_D5GM

Description: Cell line BY-2 is a Spontaneously immortalized cell line with a species of origin Nicotiana tabacum (Common tobacco)

Defining Citation: PMID:7766897, PMID:10728644, PMID:12601177, PMID:14680934, PMID:14688094, PMID:15638240, PMID:15818563, PMID:16187337, PMID:16664453, PMID:17406390, PMID:17406517, PMID:17947977, PMID:18567891, PMID:22055919, PMID:22928883, PMID:29982119, PMID:37642778

Comments: Anecdotal: This cell line was generated at the Japan Tobacco and Salt Monopoly Public Corporation. In 1982 Toshiyuki Nagata brought it with him to the USA and left it there after renaming it to NT-1 in order to distribute it clandestinely because he was not allowed officially to give the BY-2 cell line to others. He called it NT-1 as it could both mean Nicotiana tabacum or Nagata, Toshiyuki (PubMed=37642778)., Characteristics: Used as model for the electrochemical sensing of beta-glucuronidase activity of transgenic cell lines (PubMed=29982119)., Characteristics: Tested for the production of viral proteins (PubMed=14680934; PubMed=12601177; PubMed=16187337)., Characteristics: Tested for the production of synthetic proteins (PubMed=15818563)., Characteristics: Tested for the production of bacterial proteins (PubMed=14688094; PubMed=22928883)., Characteristics: Tested for the production of animal proteins (PubMed=7766897)., Characteristics: Produces low levels of nicotine (PubMed=18567891)., Characteristics: Can be used to prepare protoplasts (PubMed=17947977)., Group: Plant cell line.

Category: Spontaneously immortalized cell line

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Data and Source Information

Source: Cellosaurus

Authentication Plan

dkNET can assist you in preparing authentication plans for cell lines to comply with the NIH Submission Policy. The information can be used while planning your experiments or submitting grant applications. The authentication plan for the cell lines is based on the International Cell Line Authentication Committee (ICLAC)'s "Cell Line Checklist for Manuscripts and Grant Applications" and the example "Authentication of of Key Biological and/or Chemical Resources "(Bandrowski A). For best practices for authenticating cell lines, check our Authentication Reports services.