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URL: http://antibodyregistry.org/AB_609568
Proper Citation: (Bio-Rad Cat# OBT0030, RRID:AB_609568)
Target Antigen: RAT ANTI BrdU
Host Organism: rat
Clonality: monoclonal
Comments: manufacturer recommendations: IgG2a; IgG2a Flow Cytometry, Immunohistology - Paraffin, Immunofluorescence; Immunohistochemistry - fixed; Immunofluorescence; Flow Cytometry; Immunohistochemistry
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No rating or validation information has been found for RAT ANTI BrdU.
Possibly Non-Specific
Extracted Antibody Information: "In order to expose the proliferation markers, tissue sections were pretreated to break double-stranded DNA into single strands, by incubation in 2 N HCl in PB containing 0.3% Triton X-100 (PB-T) for 50 min. After rinsing in PB (3 × 10 min), sections were incubated overnight at 40°C in both rat anti BrdU-CldU (AbD Serotec Cat# OBT0030, RRID:AB_609568;"
Extracted Specificity Statement: "On the other hand, the rat anti-BrdU antibody, but not the mouse anti-BrdU monoclonal antibody, detects CldU in tissue samples from animals exposed to this thymidine analog (Vega and Peterson, 2005). Cross reactivity of primary antibodies with the thymidine analogs was tested by incubation of sections of CldU only and IdU only treated animals with anti-IdU or anti-CldU antibodies, respectively (followed by incubation in the corresponding secondary antibodies; Supplementary Figure 1)."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:25249943
Possibly Non-Specific
Extracted Antibody Information: "After rinsing in PB (3 × 10 min), sections were incubated overnight at 40°C in both rat anti BrdU-CldU (AbD Serotec Cat# OBT0030, RRID:AB_609568;"
Extracted Specificity Statement: "On the other hand, the rat anti-BrdU antibody, but not the mouse anti-BrdU monoclonal antibody, detects CldU in tissue samples from animals exposed to this thymidine analog (Vega and Peterson, 2005). Cross reactivity of primary antibodies with the thymidine analogs was tested by incubation of sections of CldU only and IdU only treated animals with anti-IdU or anti-CldU antibodies, respectively (followed by incubation in the corresponding secondary antibodies; Supplementary Figure 1)."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:25249943
Source: Antibody Registry
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