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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
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Nanodrop Spectrophotometer (ND-1000) for Nucleic Acid Resource Report Resource Website |
Steven Wilhelm | 10.17504/protocols.io.id2ca8e | Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from Nanodrop ND-1000 User Manual | The University of Tennessee, Knoxville | 1 | 2017 | Steven Wilhelm 2017. Nanodrop Spectrophotometer (ND-1000) for Nucleic Acid. protocols.io dx.doi.org/10.17504/protocols.io.id2ca8e | 2021-03-29 03:10:44 | |||
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Rioux Test for Catecholates Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.icrcav6 | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from Rioux, C., Jordan, D., & Rattray, J. B. (1983). Colorimetric determination of catechol siderophores in microbial cultures. Analytical Biochemistry,133(1), 163-169. doi:10.1016/0003-2697(83)90238-5 | 1 | 2017 | Dr. Steven Wilhelm 2017. Rioux Test for Catecholates. protocols.io dx.doi.org/10.17504/protocols.io.icrcav6 | 2021-03-29 03:10:55 | ||||
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Phenol-based RNA extraction from polycarbonate filters Resource Report Resource Website |
Robbie M. Martin, Steven W. Wilhelm | 10.17504/protocols.io.bivuke6w | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | This protocol is an acid-phenol-based method for extracting RNA from samples collected onto polycarbonate filters. Bead-beating is included to increase yields from difficult to lyse cells, for example, in some species of cyanobacteria. We have used it successfully in RNA-sequencing projects involving lab cultures of cyanobacteria and from freshwater and marine environmental samples. | University of Tennessee, Knoxville, University of Tennessee, Knoxville | 1 | 2020 | Robbie M. Martin, Steven W. Wilhelm 2020. Phenol-based RNA extraction from polycarbonate filters. protocols.io dx.doi.org/10.17504/protocols.io.bivuke6w | 2021-03-29 03:11:03 | |||
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Propagating T5-phages for Fluorescent Staining Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.igzcbx6 | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol. | 1 | 2017 | Dr. Steven Wilhelm 2017. Propagating T5-phages for Fluorescent Staining. protocols.io dx.doi.org/10.17504/protocols.io.igzcbx6 | 2021-03-29 03:09:28 | ||||
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Cyanobacteria Total Lipid Extraction Resource Report Resource Website |
Dr. Steven Wilhelm, Maddie Denney | 10.17504/protocols.io.ibkcakw | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Great Lakes Center for Fresh Waters and Human Health | Please contact Dr. Steven Wilhelm ([email protected]) or Maddie Denney ([email protected]) for additional information regarding this protocol.Modified from Guan, Riezman, Wenk & Riezman, 2010Please note that there are two versions of this protocol. Use the one that corresponds to your sample. | , | 1 | 2017 | Dr. Steven Wilhelm, Maddie Denney 2017. Cyanobacteria Total Lipid Extraction. protocols.io dx.doi.org/10.17504/protocols.io.ibkcakw | 2021-03-29 03:09:35 | |||
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Propidium Iodide (PI) Staining Method Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.ibxcapn | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from J Mol Biol 13,269 (1965) | 1 | 2017 | Dr. Steven Wilhelm 2017. Propidium Iodide (PI) Staining Method. protocols.io dx.doi.org/10.17504/protocols.io.ibxcapn | 2021-03-29 03:09:44 | ||||
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CT Media Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.h9kb94w | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from: Watanabe, Makoto M. "Fresh-and salt-water forms of Spirulina platensis in axenic cultures." Bull. Jpn. Soc. Phycol. 25 (1977): 371-377. | 1 | 2017 | Dr. Steven Wilhelm 2017. CT Media. protocols.io dx.doi.org/10.17504/protocols.io.h9kb94w | 2021-03-29 03:09:53 | ||||
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Using De-MetaST-BLAST Resource Report Resource Website |
Dr. Steven Wilhelm, Samantha Coy | 10.17504/protocols.io.ii2ccge | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols | Please contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol. De-MetaST-BLAST, written in C++, is a script that can validate degenerate primers by performing in silico PCR amplification of the primers against metagenomic and metatranscriptomic databases (e.g. CAMERA; MG-RAST). The output is a list of in-silco PCR amplicons that are then blasted against GenBank to determine the most homologous sequence to the amplicon. Modified from Gulvik, C. A., Effler, T. C., Wilhelm, S. W., & Buchan, A. (2012). De-MetaST-BLAST: A Tool for the Validation of Degenerate Primer Sets and Data Mining of Publicly Available Metagenomes. PLoS ONE,7(11). doi:10.1371/journal.pone.0050362 | , | 1 | 2017 | Dr. Steven Wilhelm, Samantha Coy 2017. Using De-MetaST-BLAST. protocols.io dx.doi.org/10.17504/protocols.io.ii2ccge | 2021-03-29 03:09:59 | |||
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MBBM Media Resource Report Resource Website |
Alyssa Alsante | 10.17504/protocols.io.f3dbqi6 | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | 1 | 2016 | Alyssa Alsante 2016. MBBM Media. protocols.io dx.doi.org/10.17504/protocols.io.f3dbqi6 | 2021-03-29 03:08:44 | |||||
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Running the PTC-0200 DNA Engine Resource Report Resource Website |
Dr. Steven Wilhelm, Alyssa Alsante | 10.17504/protocols.io.in7cdhn | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol. | , | 1 | 2017 | Dr. Steven Wilhelm, Alyssa Alsante 2017. Running the PTC-0200 DNA Engine. protocols.io dx.doi.org/10.17504/protocols.io.in7cdhn | 2021-03-29 03:08:46 | |||
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Using the Biomate 5 Spectrophotometer Resource Report Resource Website |
Dr. Steven Wilhelm, Samantha Coy | 10.17504/protocols.io.iktccwn | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol. | , | 1 | 2017 | Dr. Steven Wilhelm, Samantha Coy 2017. Using the Biomate 5 Spectrophotometer. protocols.io dx.doi.org/10.17504/protocols.io.iktccwn | 2021-03-29 03:08:48 | |||
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Vitamin Solution for ESAW Media for Marine Phytoplankton Resource Report Resource Website |
Ashley Humphrey | 10.17504/protocols.io.gdcbs2w | Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Stock vitamin solution for ESAW Media for Marine Phytoplankton | University of Tennessee, Knoxville | 1 | 2016 | Ashley Humphrey 2016. Vitamin Solution for ESAW Media for Marine Phytoplankton. protocols.io dx.doi.org/10.17504/protocols.io.gdcbs2w | 2021-03-29 03:08:51 | |||
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ESAW Media for Marine Phytoplankton Resource Report Resource Website |
Ashley Humphrey | 10.17504/protocols.io.f36bqre | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | University of Tennessee, Microbiology. | 1 | 2016 | Ashley Humphrey 2016. ESAW Media for Marine Phytoplankton. protocols.io dx.doi.org/10.17504/protocols.io.f36bqre | 2021-03-29 03:08:53 | ||||
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Vitamin Solution for ESAW Media for Marine Phytoplankton Resource Report Resource Website |
Ashley Humphrey | 10.17504/protocols.io.ge9bth6 | Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Stock vitamin solution for ESAW Media for Marine Phytoplankton | University of Tennessee, Knoxville | 3 | 2016 | Ashley Humphrey 2016. Vitamin Solution for ESAW Media for Marine Phytoplankton. protocols.io dx.doi.org/10.17504/protocols.io.ge9bth6 | 2021-03-29 03:08:55 | |||
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Chlorella Virus Plaque Assay Resource Report Resource Website |
Dr. Steven Wilhelm, Samantha Coy | 10.17504/protocols.io.hgqb3vw | VERVE Net, Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols | Adapted from: Van Etten, J. (n.d.). Titering of Chlorella Viruses. Retrieved from http://ncv.unl.edu/vanettenlab/ Contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol. | , | http://ncv.unl.edu/vanettenlab/Plaque%20assay.pdf | 2 | 2017 | Dr. Steven Wilhelm, Samantha Coy 2017. Chlorella Virus Plaque Assay. protocols.io dx.doi.org/10.17504/protocols.io.hgqb3vw | 2021-03-29 03:08:29 | ||
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PBS Buffer Solution Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.in9cdh6 | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol. | 1 | 2017 | Dr. Steven Wilhelm 2017. PBS Buffer Solution. protocols.io dx.doi.org/10.17504/protocols.io.in9cdh6 | 2021-03-29 03:08:30 | ||||
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A+ Media for Marine Phytoplankton Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.ibncame | Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from Wilhelm SW and CG Trick. 1995. Physiological profiles of Synechococcus (Cyanophyceae) in iron-limiting continuous cultures. Journal of Phycology, 31:79-85. | 1 | 2017 | Dr. Steven Wilhelm 2017. A+ Media for Marine Phytoplankton. protocols.io dx.doi.org/10.17504/protocols.io.ibncame | 2021-03-29 03:08:32 | ||||
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Trace Metal Solution for ESAW Media Resource Report Resource Website |
Ashley Humphrey | 10.17504/protocols.io.gdabs2e | Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Trace metals solution to be used in ESAW Media for Marine Phytoplankton | University of Tennessee, Knoxville | 1 | 2016 | Ashley Humphrey 2016. Trace Metal Solution for ESAW Media. protocols.io dx.doi.org/10.17504/protocols.io.gdabs2e | 2021-03-29 03:08:33 | |||
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BBM Media Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.h97b99n | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from Bold 1949, Bischoff and Bold 1963 | 1 | 2017 | Dr. Steven Wilhelm 2017. BBM Media. protocols.io dx.doi.org/10.17504/protocols.io.h97b99n | 2021-03-29 03:08:59 | ||||
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Guava Flow Cytometer - Troubleshooting "Tray Hold Off State" Resource Report Resource Website |
Steven Wilhelm | 10.17504/protocols.io.iv9ce96 | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol. | 1 | 2017 | Steven Wilhelm 2017. Guava Flow Cytometer - Troubleshooting "Tray Hold Off State". protocols.io dx.doi.org/10.17504/protocols.io.iv9ce96 | 2021-03-29 03:09:18 |
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