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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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MojoSort™ Mouse Neutrophil Isolation Kit Protocol Resource Report Resource Website |
Sam Li | 10.17504/protocols.io.7yuhpww | BioLegend | Product description and procedure summary:Target cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads (Cat. No. 480015/480016). The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/MojoSort_Mouse_Neutrophil_Isolation_Protocol_08072016.pdf | 2 | 2019 | Sam Li 2019. MojoSort™ Mouse Neutrophil Isolation Kit Protocol. protocols.io dx.doi.org/10.17504/protocols.io.7yuhpww | 2021-03-29 03:10:33 | ||
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MojoSort™ Human NK Cell Isolation Kit Protocol Resource Report Resource Website |
Sam Li | 10.17504/protocols.io.7ythpwn | BioLegend | Product description and procedure summary:Target cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads (Cat. No. 480015/480016). The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-nk-cell-isolation-kit-protocol/4605/ | 1 | 2019 | Sam Li 2019. MojoSort™ Human NK Cell Isolation Kit Protocol. protocols.io dx.doi.org/10.17504/protocols.io.7ythpwn | 2021-03-29 03:10:39 | ||
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Immunohistochemistry Protocol for Keratin Antibodies Resource Report Resource Website |
Sam Li | 10.17504/protocols.io.95ph85n | BioLegend | BioLegend | https://www.biolegend.com/protocols/immunohistochemistry-protocol-for-keratin-antibodies/4287/ | 3 | 2019 | Sam Li 2019. Immunohistochemistry Protocol for Keratin Antibodies. protocols.io dx.doi.org/10.17504/protocols.io.95ph85n | 2021-03-29 03:10:35 | |||
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Flex-T™ HLA Class I ELISA Protocol Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.mcsc2we | BioLegend | The HLA class I ELISA is an enzyme immunoassay based on the detection of β2-microglobulin subunit of HLA class I complexes, after capturing the complex through the conjugated biotin. To this end, biotinylated HLA class I complex is first captured in streptavidin coated microtiter wells. Subsequently, HRP-conjugated anti-human β2-microglobulin is added to detect intact HLA class I complexes. Only intact HLA class I complexes are recognized. Peptides with high affinity binding will be clearly detected by this ELISA technique, while peptides with a moderate to low binding affinity for HLA class I provide a moderate to non-detectable signal. This protocol is designed to evaluate the efficiency of peptide exchange when using the Flex-T™ system. | BioLegend | 3 | 2017 | Kelsey Miller 2017. Flex-T™ HLA Class I ELISA Protocol. protocols.io dx.doi.org/10.17504/protocols.io.mcsc2we | 2021-03-29 03:10:36 | |||
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MojoSort™ Mouse CD11c Nanobeads Protocol Resource Report Resource Website |
Sam Li | 10.17504/protocols.io.7xjhpkn | BioLegend | Product description and procedure summary: The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with the directly conjugated magnetic particles. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water.Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. Sample Preparation: Enzymatic digestion of mouse spleen is recommended to achieve the highest purity and yield of CD11c+ cells. There are several protocols published that can be applied. As a general guideline, cut mouse spleen into pieces and incubate in 0.5 mg/ml Collagenase for 30 to 60 minutes at room temperature or 37°C. Place the tube in a rocking platform with continuous agitation or gently pipette every 10 minutes. Alternatively, inject 1 ml of enzymes solution in the uncut organ. Force the tissue through a 70µm filter to prepare a single cell suspension, and wash with complete media. Resuspend cells in 0.1 mg/ml DNase 1 solution and incubate at room temperature for 10 minutes. Again, filter cells through a 70 µm filter and wash with complete media. Resuspend in complete media or MojoSort™ Buffer and keep on ice until ready to use. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-cd11c-nanobeads-protocol/4401/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse CD11c Nanobeads Protocol. protocols.io dx.doi.org/10.17504/protocols.io.7xjhpkn | 2021-03-29 03:10:37 | ||
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MojoSort™ Human CD14 Nanobeads Protocol Resource Report Resource Website |
Sam Li | 10.17504/protocols.io.7x4hpqw | BioLegend | Product description and procedure summary:The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with the directly conjugated magnetic particles. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14 mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-cd14-nanobeads-protocol/4685/ | 2 | 2019 | Sam Li 2019. MojoSort™ Human CD14 Nanobeads Protocol. protocols.io dx.doi.org/10.17504/protocols.io.7x4hpqw | 2021-03-29 03:10:55 | ||
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Chromatin Immunoprecipitation (ChIP) Assay Protocol Resource Report Resource Website |
Kelsey Knight | 10.17504/protocols.io.tj7ekrn | BioLegend | Reagents - Basic cell culture media: respective to the cell line used and experimental design Distilled water: Invitrogen® UltraPure™ Distilled Water (catalog number 19977015) or equivalent product from other vendor.Highthroughput (HT) Pro A or G 96 well plate or Spin Column (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)96-well collection plate: USA scientific® TempPlate non-skirted 96-well PCR plate natural (catalog number 1402-9596) or equivalent product from other vendor.QIAquick™ PCR purification kit (Qiagen®; catalog number 28104) or equivalent product from other vendor.Hypotonic Buffer (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)Digestion Buffer (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)Lysis Buffer (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)Column Conditioning Buffer (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)Wash Buffer 1 (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)Wash Buffer 2 (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)Wash Buffer 3 (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)Elution Buffer (Chromatrap® HT ChIP-qPCR kit; catalog number 500161 or 500162 or 500163)5M NaCl: dissolve 292g of NaCl in 800mL of H2O. Adjust the volume to 1L with H2O.1M NaHCO3: dissolve 12.6g of NaHCO3 in 100 mL of H2O. Adjust the volume to 150 mL with H2O.Protease Inhibitor Cocktail (PIC): Cell signaling Technology® (catalog number 5871) or equivalent product from other vendor.Proteinase K: Qiagen® (catalog number 19133) or equivalent product from other vendor.Proteinase K stop solution: 100mM Phenylmethylsulfonyl fluoride (PMSF). Weigh 1.742g PMSF (Sigma-Aldrich®, catalog number 10837091001) and add DMSO (Sigma-Aldrich®, catalog number D2650) to a final volume of 100mL. Dissolve completely.PBS: HyClone™ Phosphate Buffered Saline (1X) (catalog number SH30256.01) or equivalent product from other vendor.Formaldehyde: VWR® 37% w/w aqueous solution (catalog number 97064-604) or equivalent product from other vendor.0.65M Glycine: Dissolve 488g of glycine into 1L of H2O to make 6.5M glycine (10X). Dilute with PBS to make 1X before each experiment. Micrococcal Nuclease: Cell Signaling Technologies® (catalog number 10011S) or equivalent product from other vendor.1.3-1.5% Agarose gel: dissolve 1.3-1.5g of agarose powder into 100mL of TAE buffer by microwave heating. Visualize DNA under UV light by adding appropriate amount of Ethidium Bromide, or use an equivalent method.Add appropriate amount of Ethidium Bromide to cooled down gel solution to visualize DNA under UV light.0.5M EDTA: Dissolve 186.1g EDTA into 700mL of H2O. Adjust the volume to 1L with H2O.100bp ladder: NEB® (catalog number N0467S) or equivalent product from other vendor.Materials -Heat blockRotatorCentrifugeMicrocentrifuge37ºC incubatorNanodropSonicatorUV light imagerRocking platform | BioLegend | http://www.biolegend.com/media_assets/support_protocol/ChIP_Assay_Technical_Protocol_v8.pdf | 3 | 2018 | Kelsey Knight 2018. Chromatin Immunoprecipitation (ChIP) Assay Protocol. protocols.io dx.doi.org/10.17504/protocols.io.tj7ekrn | 2021-03-29 03:10:58 | ||
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Ethanol Fixation Protocol Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.e24bggw | BioLegend | Cell Fixation and Permeabilization Protocol Using 70% Ethanol | BioLegend | http://www.biolegend.com/media_assets/support_protocol/BioLegend_EthFIXPERM_032912.pdf | 1 | 2016 | Kelsey Miller 2016. Ethanol Fixation Protocol. protocols.io dx.doi.org/10.17504/protocols.io.e24bggw | 2021-03-29 03:09:36 | ||
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MojoSort™ Mouse anti-APC Nanobeads Column Protocol Resource Report Resource Website |
Sam Li | 10.17504/protocols.io.699hh96 | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-anti-apc-nanobeads-column-protocol/4759/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse anti-APC Nanobeads Column Protocol. protocols.io dx.doi.org/10.17504/protocols.io.699hh96 | 2021-03-29 03:09:42 | ||
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Immunoprecipitation Protocol Resource Report Resource Website |
Sam Li | 10.17504/protocols.io.98qh9vw | BioLegend | Immunoprecipitation is a procedure by which proteins or peptides that react specifically with an antibody are removed from solution and examined for quantity or physical characteristics. Immunoprecipitation can also be used to “enrich” a protein population prior to Western Blotting. For example, one can perform immunoprecipitation with a pan-specific antibody against a protein of interest followed by Western blotting with a modification-specific antibody (such as a phospho-specific antibody or an acetylation-specific antibody). | BioLegend | https://www.biolegend.com/protocols/immunoprecipitation-protocol/4258/ | 3 | 2019 | Sam Li 2019. Immunoprecipitation Protocol. protocols.io dx.doi.org/10.17504/protocols.io.98qh9vw | 2021-03-29 03:09:37 | ||
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MojoSort™ Selection Kits Protocol - 1 Resource Report Resource Website |
Sam Li | 10.17504/protocols.io.7yxhpxn | BioLegend | Product description and procedure summary:Target cells are depleted by incubating the sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads (Cat. No.480015/480016). The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. Note: This protocol has been optimized to remove washing steps after antibody cocktail and nanobeads incubations, resulting in a shorter and more convenient protocol. This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-selection-kits-protocol-1/4657/ | 2 | 2019 | Sam Li 2019. MojoSort™ Selection Kits Protocol - 1. protocols.io dx.doi.org/10.17504/protocols.io.7yxhpxn | 2021-03-29 03:09:50 | ||
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MojoSort™ Human NK Cell Isolation Kit Protocol Resource Report Resource Website |
Sam Li | 10.17504/protocols.io.7bihike | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-human-nk-cell-isolation-kit-column-protocol/4622/ | 1 | 2019 | Sam Li 2019. MojoSort™ Human NK Cell Isolation Kit Protocol. protocols.io dx.doi.org/10.17504/protocols.io.7bihike | 2021-03-29 03:10:00 | ||
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Western Blotting Protocol Resource Report Resource Website |
BioLegend, Inc. | 10.17504/protocols.io.e2abgae | BioLegend | , | http://www.biolegend.com/media_assets/support_protocol/WB_Analysis_BetaAmyloid_12-04-14.pdf | 1 | 2016 | BioLegend, Inc. 2016. Western Blotting Protocol. protocols.io dx.doi.org/10.17504/protocols.io.e2abgae | 2021-03-29 03:10:02 | |||
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Treg Polarization of Mouse CD4+ Cells Resource Report Resource Website |
BioLegend, Inc. | 10.17504/protocols.io.ex7bfrn | BioLegend | , | http://www.biolegend.com/media_assets/support_protocol/BioLegend_Treg_Polarization_of_Mouse_CD4_Cells_012715.pdf | 1 | 2016 | BioLegend, Inc. 2016. Treg Polarization of Mouse CD4+ Cells. protocols.io dx.doi.org/10.17504/protocols.io.ex7bfrn | 2021-03-29 03:10:03 | |||
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T Cell Activation with anti-CD3? Antibodies Resource Report Resource Website |
BioLegend, Inc. | 10.17504/protocols.io.ezybf7w | BioLegend | This is a collection of BioLegend T Cell Activation with anti-CD3ε Antibodies protocols, for human and mouse. | , | http://www.biolegend.com/media_assets/support_protocol/BioLegend_T_cell_Activation_Protocol_012715.pdf | 1 | 2016 | BioLegend, Inc. 2016. T Cell Activation with anti-CD3? Antibodies. protocols.io dx.doi.org/10.17504/protocols.io.ezybf7w | 2021-03-29 03:10:01 | ||
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MojoSort™ Isolation Kits Column Protocol - 3 Resource Report Resource Website |
Sam Li | 10.17504/protocols.io.7behije | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-isolation-kits-column-protocol-3/4591/ | 1 | 2019 | Sam Li 2019. MojoSort™ Isolation Kits Column Protocol - 3. protocols.io dx.doi.org/10.17504/protocols.io.7behije | 2021-03-29 03:10:16 | ||
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True-Nuclear™ Transcription Factor Staining Protocol for 96-Well, U-Bottom Plate Resource Report Resource Website |
BioLegend, Inc. | 10.17504/protocols.io.gq5bvy6 | BioLegend | , | http://www.biolegend.com/media_assets/support_protocol/True-Nuclear_Trademark_Transcription_Factor_Staining_Protocol.pdf | 2 | 2016 | BioLegend, Inc. 2016. True-Nuclear™ Transcription Factor Staining Protocol for 96-Well, U-Bottom Plate. protocols.io dx.doi.org/10.17504/protocols.io.gq5bvy6 | 2021-03-29 03:10:20 | |||
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Immunofluorescence Microscopy Protocol Resource Report Resource Website |
BioLegend, Inc. | 10.17504/protocols.io.eyubfww | BioLegend | , | http://www.biolegend.com/media_assets/support_protocol/Immunofluorescence_Microscopy_Protocol_050514.pdf | 1 | 2016 | BioLegend, Inc. 2016. Immunofluorescence Microscopy Protocol. protocols.io dx.doi.org/10.17504/protocols.io.eyubfww | 2021-03-29 03:10:20 | |||
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MojoSort™ Streptavidin Nanobeads Protocol - Positive Selection Resource Report Resource Website |
Sam Li | 10.17504/protocols.io.7wghpbw | BioLegend | Product description and procedure summary:If your target cells are the labeled cells (the positive fraction), use the Streptavidin Nanobeads Protocol – Positive Selection. If your target cells are the unlabeled cells (negative fraction), use the Streptavidin Nanobeads Protocol - Negative Selection. Target cells are isolated by incubating your sample with a Biotin-conjugated antibody or antibody cocktail, followed by incubation with magnetic Streptavidin Nanobeads (Cat. No. 480015/480016). The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14 mL tubes and Magnet, and place the tube in the magnet for 10 minutes. | BioLegend | https://www.biolegend.com/protocols/mojosort-streptavidin-nanobeads-protocol-positive-selection/4748/ | 1 | 2019 | Sam Li 2019. MojoSort™ Streptavidin Nanobeads Protocol - Positive Selection. protocols.io dx.doi.org/10.17504/protocols.io.7wghpbw | 2021-03-29 03:10:28 | ||
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MojoSort™ Mouse Neutrophil Isolation Kit Column Protocol Resource Report Resource Website |
Sam Li | 10.17504/protocols.io.7bjhikn | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend | https://www.biolegend.com/protocols/mojosort-mouse-neutrophil-isolation-kit-column-protocol/4770/ | 1 | 2019 | Sam Li 2019. MojoSort™ Mouse Neutrophil Isolation Kit Column Protocol. protocols.io dx.doi.org/10.17504/protocols.io.7bjhikn | 2021-03-29 03:10:30 |
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