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Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: For Transcriptomics During One-Step Growth Curves for Cellulophaga Phages protocol.
Proper citation: Matthew Sullivan 2016. Centrifuged Plaque Assay Sample Steps. protocols.io dx.doi.org/10.17504/protocols.io.dep3dm Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Titration of AmPure XP Beads for Removal of Fragments . protocols.io dx.doi.org/10.17504/protocols.io.c52y8d Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: For One-step growth curves for Cellulophaga phages protocol and Transcriptomics During One-Step Growth Curves for Cellulophaga Phages protocol.
Proper citation: Matthew Sullivan 2016. Cellulophaga growth reading. protocols.io dx.doi.org/10.17504/protocols.io.dpa5id Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. 16S Universal Bacterial PCR. protocols.io dx.doi.org/10.17504/protocols.io.dfr3m5 Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Transcriptomics During One-Step Growth Curves for Cellulophaga Phages. protocols.io dx.doi.org/10.17504/protocols.io.dem3c5 Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. DNA Precipitation Protocol. protocols.io dx.doi.org/10.17504/protocols.io.c34yqv Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: Modified after Glöckner et al. 1999
Proper citation: Matthew Sullivan 2016. Fixation of Planktonic Samples. protocols.io dx.doi.org/10.17504/protocols.io.c3ayid Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Plating Prochlorococcus and Synechococcus strains in top agarose for plaque assays. protocols.io dx.doi.org/10.17504/protocols.io.c3vyn5 Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. g23 T4-type (myovirus) PCR Protocol. protocols.io dx.doi.org/10.17504/protocols.io.dfq3mv Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: From Sullivan M., Lindell D., Lee J., Thompson L., Bielawski J., Chisholm S. Prevalence and Evolution of Core Photosystem II Genes in Marine Cyanobacterial Viruses and Their Hosts.PLOS Biology, 2006 4(8):e234. Please see the published manuscript for additional information.
Proper citation: Matthew Sullivan 2016. Amplification of phage genes from lysates and environmental samples. protocols.io dx.doi.org/10.17504/protocols.io.djn4md Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: The usefulness of cesium chloride (CsCl) step gradients and continuous gradients for the separation of viruses is based on the differing buoyant densities of viruses, bacteria, and extracellular debris. This protocol provides a method for Cesium Chloride and DNA Extraction for Viruses (See guidelines for DNA Extraction).
Proper citation: Matthew Sullivan 2016. Cesium Chloride Gradients. protocols.io dx.doi.org/10.17504/protocols.io.c7dzi5 Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: For One-step growth curves for Cellulophaga phages protocol.
Proper citation: Matthew Sullivan 2016. Centrifuged Sample Steps. protocols.io dx.doi.org/10.17504/protocols.io.ddj24m Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: Can be used for diluting or resuspending phage preparations.
Proper citation: Matthew Sullivan 2015. Phage Buffer. protocols.io dx.doi.org/10.17504/protocols.io.c5ey3d Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Host Range Protocols. protocols.io dx.doi.org/10.17504/protocols.io.dew3fd Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: For use in Wet-mount Method for Enumeration of Aquatic Viruses
Proper citation: Matthew Sullivan 2016. Working Bead Solution. protocols.io dx.doi.org/10.17504/protocols.io.c84zyv Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Media Recipes. protocols.io dx.doi.org/10.17504/protocols.io.deq3dv Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. DNA Extraction Protocol. protocols.io dx.doi.org/10.17504/protocols.io.c32yqd Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: This mixture is is used with Cesium Chloride Dialysis for Viruses
Proper citation: Matthew Sullivan 2016. MTN100 with 1x NaCl. protocols.io dx.doi.org/10.17504/protocols.io.def3bm Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: This mixture is used for the DNA Extraction Protocol.
Proper citation: Matthew Sullivan 2016. CsCl purified phage lysate, Proteinase K, SDS. protocols.io dx.doi.org/10.17504/protocols.io.c68zhv Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Screening Recombinant Clones by PCR. protocols.io dx.doi.org/10.17504/protocols.io.c48yzv Copy
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