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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
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Flex-T™ HLA Class I ELISA Protocol Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.mcsc2we | BioLegend | The HLA class I ELISA is an enzyme immunoassay based on the detection of β2-microglobulin subunit of HLA class I complexes, after capturing the complex through the conjugated biotin. To this end, biotinylated HLA class I complex is first captured in streptavidin coated microtiter wells. Subsequently, HRP-conjugated anti-human β2-microglobulin is added to detect intact HLA class I complexes. Only intact HLA class I complexes are recognized. Peptides with high affinity binding will be clearly detected by this ELISA technique, while peptides with a moderate to low binding affinity for HLA class I provide a moderate to non-detectable signal. This protocol is designed to evaluate the efficiency of peptide exchange when using the Flex-T™ system. | BioLegend | 3 | 2017 | Kelsey Miller 2017. Flex-T™ HLA Class I ELISA Protocol. protocols.io dx.doi.org/10.17504/protocols.io.mcsc2we | 2021-03-29 03:10:36 | |||
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MojoSort™ Human CD45 Nanobeads Protocol B Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.e22bgge | Note: If the percentage of CD45+ cells in your sample is less than 50%, please follow Protocol A. If it is higher than 50% then please follow protocol B.The cells targeted by the Nanobeads are either selected or depleted by incubating your sample with the directly conjugated magnetic particles. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/MojoSort_Human_CD45_Nanobeads_Protocol_12182015.pdf | 1 | 2016 | Kelsey Miller 2016. MojoSort™ Human CD45 Nanobeads Protocol B. protocols.io dx.doi.org/10.17504/protocols.io.e22bgge | 2021-03-29 03:10:47 | |||
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Ethanol Fixation Protocol Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.e24bggw | BioLegend | Cell Fixation and Permeabilization Protocol Using 70% Ethanol | BioLegend | http://www.biolegend.com/media_assets/support_protocol/BioLegend_EthFIXPERM_032912.pdf | 1 | 2016 | Kelsey Miller 2016. Ethanol Fixation Protocol. protocols.io dx.doi.org/10.17504/protocols.io.e24bggw | 2021-03-29 03:09:36 | ||
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MojoSort™ Streptavidin Nanobeads Protocol Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.e2rbgd6 | BioLegend | The cells targeted by the Streptavidin Nanobeads are either selected or depleted by incubating your sample with the magnetic particles after incubating with a biotin-conjugated antibody or antibody cocktail. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/MojoSort_Streptavidin_Nanobeads_Protocol_V02_10192015.pdf | 1 | 2016 | Kelsey Miller 2016. MojoSort™ Streptavidin Nanobeads Protocol. protocols.io dx.doi.org/10.17504/protocols.io.e2rbgd6 | 2021-03-29 03:08:48 | ||
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MojoSort™ Streptavidin Nanobeads Column Protocol - Positive Selection Resource Report Resource Website |
Kelsey Miller, Sam Li | 10.17504/protocols.io.692hh8e | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend, BioLegend | https://www.biolegend.com/protocols/mojosort-streptavidin-nanobeads-column-protocol-positive-selection/4773/ | 2 | 2019 | Kelsey Miller, Sam Li 2019. MojoSort™ Streptavidin Nanobeads Column Protocol - Positive Selection. protocols.io dx.doi.org/10.17504/protocols.io.692hh8e | 2021-03-29 03:08:49 | ||
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Th9 Polarization of Mouse CD4+ Cells Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.tnnemde | BioLegend | BioLegend | https://www.biolegend.com/protocols/th9-polarization-of-mouse-cd4--cells-protocol/4243/ | 2 | 2018 | Kelsey Miller 2018. Th9 Polarization of Mouse CD4+ Cells. protocols.io dx.doi.org/10.17504/protocols.io.tnnemde | 2021-03-29 03:08:52 | |||
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Flex-T™ HLA Class I ELISA Protocol Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.e7ebhje | BioLegend | The HLA class I ELISA is an enzyme immunoassay based on the detection of β2-microglobulin subunit of HLA class I complexes, after capturing the complex through the conjugated biotin. To this end, biotinylated HLA class I complex is first captured in streptavidin coated microtiter wells. Subsequently, HRP-conjugated anti-human β2-microglobulin is added to detect intact HLA class I complexes. Only intact HLA class I complexes are recognized. Peptides with high affinity binding will be clearly detected by this ELISA technique, while peptides with a moderate to low binding affinity for HLA class I provide a moderate to non-detectable signal. This protocol is designed to evaluate the efficiency of peptide exchange when using the Flex-T™ system. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/Protocol%20for%20HLA%20class%20I%20ELISA%2006202016.pdf | 1 | 2016 | Kelsey Miller 2016. Flex-T™ HLA Class I ELISA Protocol. protocols.io dx.doi.org/10.17504/protocols.io.e7ebhje | 2021-03-29 03:08:30 | ||
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Cell Fixation and Permeabilization Protocol using 70% Ethanol Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.hv3b68n | BioLegend | Cell Fixation and Permeabilization Protocol Using 70% Ethanol | BioLegend | http://www.biolegend.com/media_assets/support_protocol/BioLegend_EthFIXPERM_032912.pdf | 2 | 2017 | Kelsey Miller 2017. Cell Fixation and Permeabilization Protocol using 70% Ethanol. protocols.io dx.doi.org/10.17504/protocols.io.hv3b68n | 2021-03-29 03:08:40 | ||
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Immunohistochemistry Protocol for Sternberger Monoclonal Antibodies Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.e2jbgcn | BioLegend | BioLegend | http://www.biolegend.com/media_assets/support_protocol/Sternberger_Monoclonals_IHC_Protocol_using_USA_Kit_102914.pdf | 1 | 2016 | Kelsey Miller 2016. Immunohistochemistry Protocol for Sternberger Monoclonal Antibodies. protocols.io dx.doi.org/10.17504/protocols.io.e2jbgcn | 2021-03-29 03:08:03 | |||
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Immunohistochemistry Protocol for Ultra Streptavidin Detection Kits (USA) Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.fq9bmz6 | BioLegend | BioLegend | http://www.biolegend.com/media_assets/support_protocol/IHC_Protocol_for_Ultra_Streptavidin_Detection_Kits_USA_090815.pdf | 2 | 2016 | Kelsey Miller 2016. Immunohistochemistry Protocol for Ultra Streptavidin Detection Kits (USA). protocols.io dx.doi.org/10.17504/protocols.io.fq9bmz6 | 2021-03-29 03:08:00 | |||
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Immunofluorescence Microscopy Protocol with Methanol Fixed Cells Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.hv9b696 | BioLegend | BioLegend | 2 | 2017 | Kelsey Miller 2017. Immunofluorescence Microscopy Protocol with Methanol Fixed Cells. protocols.io dx.doi.org/10.17504/protocols.io.hv9b696 | 2021-03-29 03:08:16 | ||||
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MojoSort™ Streptavidin Nanobeads Column Protocol - Negative Selection Resource Report Resource Website |
Kelsey Miller, Sam Li | 10.17504/protocols.io.693hh8n | BioLegend | BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed. | BioLegend, BioLegend | https://www.biolegend.com/protocols/mojosort-streptavidin-nanobeads-column-protocol-negative-selection/4772/ | 2 | 2019 | Kelsey Miller, Sam Li 2019. MojoSort™ Streptavidin Nanobeads Column Protocol - Negative Selection. protocols.io dx.doi.org/10.17504/protocols.io.693hh8n | 2021-03-29 03:08:17 | ||
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Immunohistochemistry Protocol for Keratin Antibodies Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.tkhekt6 | BioLegend | BioLegend | https://www.biolegend.com/protocols/immunohistochemistry-protocol-for-keratin-antibodies/4287/ | 2 | 2018 | Kelsey Miller 2018. Immunohistochemistry Protocol for Keratin Antibodies. protocols.io dx.doi.org/10.17504/protocols.io.tkhekt6 | 2021-03-29 03:08:25 | |||
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Propidium Iodide Cell Cycle Staining Protocol Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.e2mbgc6 | BioLegend | BioLegend | http://www.biolegend.com/media_assets/support_protocol/PI_Cell_Cycle_Staining_Protocol_V03_04272016.pdf | 1 | 2016 | Kelsey Miller 2016. Propidium Iodide Cell Cycle Staining Protocol. protocols.io dx.doi.org/10.17504/protocols.io.e2mbgc6 | 2021-03-29 03:07:35 | |||
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Anti-Neu5Gc Antibody Kit Protocol - ELISA Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.hvpb65n | BioLegend | This protocol is essentially the same as a standard ELISA protocol, with one major exception – all animal compounds must be avoided in buffers, etc. because they contain Neu5Gc, which will interfere with antibody activity and result in misleading data. Essentially, the glycoconjugate to be evaluated is immobilized on an ELISA plate and exposed to a primary antibody. The antibody provided in this kit has been shown to identify as little as 5 pmol of Neu5Gc per microgram glycoprotein, which is at or below the current detection limit for conventional DMB HPLC analysis. An advantage of the ELISA procedure is that it confirms the presence of Neu5Gc on the glycoconjugate of interest, but use caution, as it also reacts with solution/media that contains glycoconjugate. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/Neu5Gc_Antibody_Kit_Protocols_110813.pdf | 2 | 2017 | Kelsey Miller 2017. Anti-Neu5Gc Antibody Kit Protocol - ELISA. protocols.io dx.doi.org/10.17504/protocols.io.hvpb65n | 2021-03-29 03:07:38 | ||
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Anti-BrdU Staining Using 70% Ethanol and 2N HCl Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.hvmb646 | BioLegend | Anti-BrdU Staining Protocol Using 70% Ethanol and 2N HCl | BioLegend | http://www.biolegend.com/media_assets/support_protocol/BrdU_Staining_Protocols_05102016.pdf | 2 | 2017 | Kelsey Miller 2017. Anti-BrdU Staining Using 70% Ethanol and 2N HCl. protocols.io dx.doi.org/10.17504/protocols.io.hvmb646 | 2021-03-29 03:11:35 | ||
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Anti-Neu5Gc Antibody Kit Protocols Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.e25bgg6 | BioLegend | Anti-Neu5Gc Antibody Kit Protocols for Flow Cytometry, ELISA, and Western blot. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/Neu5Gc_Antibody_Kit_Protocols_110813.pdf | 1 | 2016 | Kelsey Miller 2016. Anti-Neu5Gc Antibody Kit Protocols. protocols.io dx.doi.org/10.17504/protocols.io.e25bgg6 | 2021-03-29 03:13:29 | ||
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Anti-Neu5Gc Antibody Kit Protocol - Western blot Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.e28bghw | BioLegend | The Anti-Neu5Gc Antibody Kit contains the essential monospecific polyclonal chicken IgY antibody, along with a negative control primary antibody to detect the presence of Neu5Gc on glycoconjugates by Western blot (WB). Samples to be evaluated are first subjected to SDS-PAGE, followed by transfer to a nitrocellulose or polyvinylidenedifluoride (PVDF) membrane. The membrane is then incubated with affinity-purified polyclonal anti-Neu5Gc to determine the presence of Neu5Gc on the protein of interest.The antibody provided in this kit has been shown to identify as little as 5 pmol of Neu5Gc per ug glycoprotein, which is at or below the current detection limit for conventional analysis by acid release, purification, DMB derivatization, HPLC, and electrospray mass-spectrometry. The Western blot provides additional information in that it confirms that Neu5G is directly linked to the glycoprotein of interest rather than to an accompanying sample component. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/Neu5Gc_Antibody_Kit_Protocols_110813.pdf | 1 | 2016 | Kelsey Miller 2016. Anti-Neu5Gc Antibody Kit Protocol - Western blot. protocols.io dx.doi.org/10.17504/protocols.io.e28bghw | 2021-03-29 03:13:34 | ||
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Intracellular Staining With True-Phos™ Perm Buffer in Cell Suspensions Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.e2fbgbn | BioLegend | BioLegend | http://www.biolegend.com/media_assets/support_protocol/Intracellular_Staining_with_TruePhos_Buffer_in_Cell_Suspensions_05052016v2_DMS_Update.pdf | 1 | 2016 | Kelsey Miller 2016. Intracellular Staining With True-Phos™ Perm Buffer in Cell Suspensions. protocols.io dx.doi.org/10.17504/protocols.io.e2fbgbn | 2021-03-29 03:12:48 | |||
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MojoSort™ Mouse Neutrophil Isolation Kit Protocol Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.fxnbpme | BioLegend | This procedure is optimized for the isolation of 107 to 1 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water.Product description and procedure summary:Non Neutrophil cells are depleted by incubating the sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/MojoSort_Mouse_Neutrophil_Isolation_Protocol_08072016.pdf | 1 | 2016 | Kelsey Miller 2016. MojoSort™ Mouse Neutrophil Isolation Kit Protocol. protocols.io dx.doi.org/10.17504/protocols.io.fxnbpme | 2021-03-29 03:14:35 |
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