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Authors: Steven Wilhelm
Group: Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from Nanodrop ND-1000 User Manual
Proper citation: Steven Wilhelm 2017. Nanodrop Spectrophotometer (ND-1000) for Nucleic Acid. protocols.io dx.doi.org/10.17504/protocols.io.id2ca8e Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from Rioux, C., Jordan, D., & Rattray, J. B. (1983). Colorimetric determination of catechol siderophores in microbial cultures. Analytical Biochemistry,133(1), 163-169. doi:10.1016/0003-2697(83)90238-5
Proper citation: Dr. Steven Wilhelm 2017. Rioux Test for Catecholates. protocols.io dx.doi.org/10.17504/protocols.io.icrcav6 Copy
Authors: Robbie M. Martin, Steven W. Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: This protocol is an acid-phenol-based method for extracting RNA from samples collected onto polycarbonate filters. Bead-beating is included to increase yields from difficult to lyse cells, for example, in some species of cyanobacteria. We have used it successfully in RNA-sequencing projects involving lab cultures of cyanobacteria and from freshwater and marine environmental samples.
Proper citation: Robbie M. Martin, Steven W. Wilhelm 2020. Phenol-based RNA extraction from polycarbonate filters. protocols.io dx.doi.org/10.17504/protocols.io.bivuke6w Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.
Proper citation: Dr. Steven Wilhelm 2017. Propagating T5-phages for Fluorescent Staining. protocols.io dx.doi.org/10.17504/protocols.io.igzcbx6 Copy
Authors: Dr. Steven Wilhelm, Maddie Denney
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Great Lakes Center for Fresh Waters and Human Health
Summary: Please contact Dr. Steven Wilhelm ([email protected]) or Maddie Denney ([email protected]) for additional information regarding this protocol.Modified from Guan, Riezman, Wenk & Riezman, 2010Please note that there are two versions of this protocol. Use the one that corresponds to your sample.
Proper citation: Dr. Steven Wilhelm, Maddie Denney 2017. Cyanobacteria Total Lipid Extraction. protocols.io dx.doi.org/10.17504/protocols.io.ibkcakw Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from J Mol Biol 13,269 (1965)
Proper citation: Dr. Steven Wilhelm 2017. Propidium Iodide (PI) Staining Method. protocols.io dx.doi.org/10.17504/protocols.io.ibxcapn Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from: Watanabe, Makoto M. "Fresh-and salt-water forms of Spirulina platensis in axenic cultures." Bull. Jpn. Soc. Phycol. 25 (1977): 371-377.
Proper citation: Dr. Steven Wilhelm 2017. CT Media. protocols.io dx.doi.org/10.17504/protocols.io.h9kb94w Copy
Authors: Dr. Steven Wilhelm, Samantha Coy
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols
Summary: Please contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol.
De-MetaST-BLAST, written in C++, is a script that can validate degenerate primers by performing in silico PCR amplification of the primers against metagenomic and metatranscriptomic databases (e.g. CAMERA; MG-RAST). The output is a list of in-silco PCR amplicons that are then blasted against GenBank to determine the most homologous sequence to the amplicon.
Modified from Gulvik, C. A., Effler, T. C., Wilhelm, S. W., & Buchan, A. (2012). De-MetaST-BLAST: A Tool for the Validation of Degenerate Primer Sets and Data Mining of Publicly Available Metagenomes. PLoS ONE,7(11). doi:10.1371/journal.pone.0050362
Proper citation: Dr. Steven Wilhelm, Samantha Coy 2017. Using De-MetaST-BLAST. protocols.io dx.doi.org/10.17504/protocols.io.ii2ccge Copy
Authors: Alyssa Alsante
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Proper citation: Alyssa Alsante 2016. MBBM Media. protocols.io dx.doi.org/10.17504/protocols.io.f3dbqi6 Copy
Authors: Dr. Steven Wilhelm, Alyssa Alsante
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.
Proper citation: Dr. Steven Wilhelm, Alyssa Alsante 2017. Running the PTC-0200 DNA Engine. protocols.io dx.doi.org/10.17504/protocols.io.in7cdhn Copy
Authors: Dr. Steven Wilhelm, Samantha Coy
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.
Proper citation: Dr. Steven Wilhelm, Samantha Coy 2017. Using the Biomate 5 Spectrophotometer. protocols.io dx.doi.org/10.17504/protocols.io.iktccwn Copy
Authors: Ashley Humphrey
Group: Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Stock vitamin solution for ESAW Media for Marine Phytoplankton
Proper citation: Ashley Humphrey 2016. Vitamin Solution for ESAW Media for Marine Phytoplankton. protocols.io dx.doi.org/10.17504/protocols.io.gdcbs2w Copy
Authors: Ashley Humphrey
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Proper citation: Ashley Humphrey 2016. ESAW Media for Marine Phytoplankton. protocols.io dx.doi.org/10.17504/protocols.io.f36bqre Copy
Authors: Ashley Humphrey
Group: Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Stock vitamin solution for ESAW Media for Marine Phytoplankton
Proper citation: Ashley Humphrey 2016. Vitamin Solution for ESAW Media for Marine Phytoplankton. protocols.io dx.doi.org/10.17504/protocols.io.ge9bth6 Copy
Authors: Dr. Steven Wilhelm, Samantha Coy
Group: VERVE Net, Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols
Summary: Adapted from: Van Etten, J. (n.d.). Titering of Chlorella Viruses. Retrieved from http://ncv.unl.edu/vanettenlab/
Contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol.
Proper citation: Dr. Steven Wilhelm, Samantha Coy 2017. Chlorella Virus Plaque Assay. protocols.io dx.doi.org/10.17504/protocols.io.hgqb3vw Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.
Proper citation: Dr. Steven Wilhelm 2017. PBS Buffer Solution. protocols.io dx.doi.org/10.17504/protocols.io.in9cdh6 Copy
Authors: Dr. Steven Wilhelm
Group: Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from Wilhelm SW and CG Trick. 1995. Physiological profiles of Synechococcus (Cyanophyceae) in iron-limiting continuous cultures. Journal of Phycology, 31:79-85.
Proper citation: Dr. Steven Wilhelm 2017. A+ Media for Marine Phytoplankton. protocols.io dx.doi.org/10.17504/protocols.io.ibncame Copy
Authors: Ashley Humphrey
Group: Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Trace metals solution to be used in ESAW Media for Marine Phytoplankton
Proper citation: Ashley Humphrey 2016. Trace Metal Solution for ESAW Media. protocols.io dx.doi.org/10.17504/protocols.io.gdabs2e Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from Bold 1949, Bischoff and Bold 1963
Proper citation: Dr. Steven Wilhelm 2017. BBM Media. protocols.io dx.doi.org/10.17504/protocols.io.h97b99n Copy
Authors: Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.
Proper citation: Steven Wilhelm 2017. Guava Flow Cytometer - Troubleshooting "Tray Hold Off State". protocols.io dx.doi.org/10.17504/protocols.io.iv9ce96 Copy
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