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Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The kit formulation allows for efficient capped RNA synthesis using cap analog (ARCA).
Proper citation: New England Biolabs 2015. Capped RNA Synthesis (E2050). protocols.io dx.doi.org/10.17504/protocols.io.cryv7v Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of RNA into high quality directional (strand-specific) libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow has minimal hands-on time and is compatible with poly(A) mRNA enrichment and rRNA depletion methods.
Proper citation: New England Biolabs 2018. Protocol for use with Purified mRNA or rRNA Depleted RNA and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765). protocols.io dx.doi.org/10.17504/protocols.io.t8xerxn Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Q5U Hot Start High-Fidelity DNA Polymerase is a modified version of Q5®High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3′ to 5′ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.
Proper citation: New England Biolabs 2020. PCR Using Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515): General PCR, USER®Cloning, dUTP incorporation/Carryover prevention. protocols.io dx.doi.org/10.17504/protocols.io.7schnaw Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Radioactive T4PNK Minus Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cqavsd Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Guidelines for Primer Design for Restriction Enzyme Cloning (E6901)
Proper citation: New England Biolabs 2015. Primer Design for Restriction Enzyme Cloning (E6901). protocols.io dx.doi.org/10.17504/protocols.io.cpnvmd Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2020. Protocol for Standard RNA Synthesis with Hi-T7 RNA Polymerase (NEB #M0658). protocols.io dx.doi.org/10.17504/protocols.io.8tnhwme Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2020. Thermolabile Proteinase K Typical Reaction Protocol. protocols.io dx.doi.org/10.17504/protocols.io.7r6hm9e Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for dephosphorylation of 5'-ends of DNA using AnP (Antarctic Phosphatase - M0289).
Proper citation: New England Biolabs 2017. Dephosphorylation of 5' -ends of DNA using AnP (M0289). protocols.io dx.doi.org/10.17504/protocols.io.irtcd6n Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB), Coronavirus Method Development Community
Summary: This protocol utilizes the Monarch Total RNA Miniprep Kit to purify RNA from buccal swabs, nasopharyngeal samples, and saliva.
Proper citation: New England Biolabs 2020. RNA Purification from Buccal Swabs, Nasopharyngeal Samples (swab or aspirate) and Saliva using the Monarch Total RNA Miniprep Kit. protocols.io dx.doi.org/10.17504/protocols.io.be93jh8n Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Mixture for M0273 Taq PCR. protocols.io dx.doi.org/10.17504/protocols.io.cicuav Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2014. 10X RT Buffer. protocols.io dx.doi.org/10.17504/protocols.io.cjwupd Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocols is for PCR using Q5® High-Fidelity DNA Polymerase (M0491)
Proper citation: New England Biolabs 2015. PCR with Q5® Polymerase (M0491). protocols.io dx.doi.org/10.17504/protocols.io.cidua5 Copy
Authors: New England Biolabs, Menna Teffera
Group: New England Biolabs (NEB)
Summary: This module is part of the Ultra™ II workflow, and is optimized for use with the NEBNext®Ultra II End Repair/dA-Tailing Module (NEB #E7546), for Illumina®-compatible library construction.The NEBNext Ultra II Ligation Module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546) or the NEBNext Ultra II FS DNA Module (NEB #E7810).
Proper citation: New England Biolabs, Menna Teffera 2019. NEBNext Ultra II Ligation Module (NEB # E7595) for NEBNext Ultra II FS DNA Module (NEB # E7810). protocols.io dx.doi.org/10.17504/protocols.io.4ntgven Copy
Authors: NEB
Group: New England Biolabs (NEB)
Summary: This is the synthesis protocol for modified nucleotides using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (E2050). The kit is capable of synthesizing biotin- or dye-modified RNA.
Proper citation: NEB 2015. RNA Synthesis with Modified Nucleotides (E2050). protocols.io dx.doi.org/10.17504/protocols.io.cv7w9m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) depletes both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochodrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g. FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications.
Proper citation: New England Biolabs 2018. NEBNext rRNA Depletion Kit (Human/Mouse/Rat) E6310. protocols.io dx.doi.org/10.17504/protocols.io.mzkc74w Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Quick CIP is a heat-labile version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source.Rapid and irreversible heat inactivation eliminates unwanted activityImproved storage stability versus native enzymeFaster reaction setup (no supplemental additives like zinc required) and shorter incubation timeFlexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)Less enzyme required (high specific activity), resulting in a lower cost per reactionNo need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysisRecombinant for purity, consistency and value
Proper citation: New England Biolabs 2020. Protocol for Dephosphorylation of 5′ ends of DNA using Quick CIP (NEB #M0525). protocols.io dx.doi.org/10.17504/protocols.io.7r2hm8e Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. T4 PNK Radioactive Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cp8vrv Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This transformation protocol is to be performed directly in the C2527H tubes. (For the C2527I protocol, see here.)
Proper citation: New England Biolabs 2015. Transformation Protocol for BL21(DE3) Competent Cells (C2527H). protocols.io dx.doi.org/10.17504/protocols.io.criv4d Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This transformation protocol is for the C2527I cells. (For the C2527H protocol, see here.)
Proper citation: New England Biolabs 2015. Transformation Protocol for BL21(DE3) Competent Cells (C2527I). protocols.io dx.doi.org/10.17504/protocols.io.crjv4m Copy
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