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Authors: Isabel Gautreau
Group: New England Biolabs (NEB)
Summary: This protocol is a group of the following 5 methods from the NEB website:1. NEBNext End Prep 2. Adaptor Ligation3. Size Selection of Adaptor-ligated DNA4. PCR Amplification5. Cleanup of PCR Amplification
Proper citation: Isabel Gautreau 2015. NEBNext® Ultra™ DNA Library Prep Protocol for Illumina® With Size Selection (E7370). protocols.io dx.doi.org/10.17504/protocols.io.cg5ty5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for immunoprecipitation using Protein A/G Magnetic Beads.
Proper citation: New England Biolabs 2015. Immunoprecipitation using Protein A/G Magnetic Beads. protocols.io dx.doi.org/10.17504/protocols.io.crav2d Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Please see the NEB website for more information.
Proper citation: New England Biolabs 2015. Ligation Protocol WITH T4 DNA Ligase (M0202). protocols.io dx.doi.org/10.17504/protocols.io.cdks4v Copy
Authors: Isabel Gautreau
Group: New England Biolabs (NEB)
Summary: This protocol is a group of the following 5 methods from the NEB website:1. NEBNext End Prep 2. Adaptor Ligation3. Size Selection of Adaptor-ligated DNA4. PCR Amplification5. Cleanup of PCR Amplification
Proper citation: Isabel Gautreau 2017. NEBNext® Ultra™ DNA Library Prep Protocol for Illumina® With Size Selection (E7370). protocols.io dx.doi.org/10.17504/protocols.io.gx8bxrw Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Radioactive Labeling with T4 Polynucleotide Kinase
Proper citation: New England Biolabs 2015. Radioactive Labeling with T4 PNK (M0201S). protocols.io dx.doi.org/10.17504/protocols.io.cp7vrm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010).Quickly and easily purify up to 100 µg of high-quality total RNA from multiple sample types – all with one kit!For use with blood, cells and tissuesAlso works with tough to lyse samples (bacteria, yeast, plant)Effectively purifies total RNA of all sizes, including small RNAs >20 ntEfficient genomic DNA removal (column and DNase I-based)Contains Proteinase K for processing of tissues and blood samplesIncludes RNA Protection Reagent for sample preservationExcellent valueKit components available separately
Proper citation: New England Biolabs 2018. Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010). protocols.io dx.doi.org/10.17504/protocols.io.p26dqhe Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Cas9 Nuclease, S. pyogenes (Cas9), is a double-stranded DNA endonuclease that is guided to its target by sequence complementarity of a small RNA loaded into the protein. This protocol describes how to digest double-stranded DNA in vitro using Cas9 and a single guide RNA (sgRNA).
Proper citation: New England Biolabs 2014. In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386). protocols.io dx.doi.org/10.17504/protocols.io.ch2t8d Copy
Authors: Menna Teffera, New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II End Repair/dA-Tailing Module is optimized to convert 500 pg-1 μg of fragmented DNA to repaired DNA having 5 ́ phosphorylated, 3 ́ dA-tailed ends. This module is part of the Ultra™ II workflow, and is optimized for use with the NEBNext™ Ultra II Ligation Module (NEB #E7595), for Illumina®-compatible library construction.
This module is also compatible with some Oxford Nanopore MinION™workflows.This module is designed for use with NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7600 or #E7535), NEBNext Ultra II Ligation Module (NEB #E7595), and NEBNext Ultra II Q5 Master Mix (NEB #M0544). Kits that include reagents for every step in the Ultra II DNA library construction workflow are also available (NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) and NEBNext Ultra II DNA Library Prep with Sample Purification Beads (NEB #E7103).
Proper citation: Menna Teffera, New England Biolabs 2019. NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB #E7546). protocols.io dx.doi.org/10.17504/protocols.io.4nngvde Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocol is for dephosphorylation of 5´-ends of DNA using rSAP in restriction enzyme reaction (M0371)
Proper citation: New England Biolabs 2018. Dephosphorylation using rSAP in Restriction Enzyme Reaction(M0371). protocols.io dx.doi.org/10.17504/protocols.io.nkxdcxn Copy
Authors: Breton Hornblower
Group: New England Biolabs (NEB)
Summary: Cas9 Nuclease, S. pyogenes (Cas9), is a double-stranded DNA endonuclease that is guided to its target by sequence complementarity of a small RNA loaded into the protein. This protocol describes how to digest double-stranded DNA in vitro using Cas9 and a single guide RNA (sgRNA).
Proper citation: Breton Hornblower 2017. In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386). protocols.io dx.doi.org/10.17504/protocols.io.hwkb7cw Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is a generic PNGase F protocol with non-denaturing reaction conditions. It is appropriate for both P0704 and P0708. Typical reaction conditions are below.
Proper citation: New England Biolabs 2015. PNGase F Protocol, Non-Denaturing Conditions. protocols.io dx.doi.org/10.17504/protocols.io.cqivud Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Protocol for blunting ends by 3' overhang removal and fill-in of 3' recessed (5' overhang) ends using T4 DNA Polymerase.
Proper citation: New England Biolabs 2018. Blunting Protocol (M0203). protocols.io dx.doi.org/10.17504/protocols.io.iswcefe Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2014. FirstStrand with RT Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cjvun5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Standard RNA Synthesis Mixture for E2050. protocols.io dx.doi.org/10.17504/protocols.io.cr3v8m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the reaction for the "Ligation Protocol with T4 DNA Ligase"
Proper citation: New England Biolabs 2015. T4 DNA Ligase Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cf7trm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the "quick" version of Monarch® PCR & DNA Cleanup Kit (5 μg) Protocol (NEB #T1030). For the full protocol, please click here.
Proper citation: New England Biolabs 2018. Quick Protocol for Monarch® PCR & DNA Cleanup Kit (5 ?g) (NEB #T1030). protocols.io dx.doi.org/10.17504/protocols.io.n3vdgn6 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Please see the NEB website for more information.
Proper citation: New England Biolabs 2017. Ligation Protocol with T4 DNA Ligase (M0202). protocols.io dx.doi.org/10.17504/protocols.io.irscd6e Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocol is for dephosphorylation of 5´-ends of DNA using rSAP in restriction enzyme reaction (M0371)
Proper citation: New England Biolabs 2015. Dephosphorylation using rSAP in Restriction Enzyme Reaction(M0371). protocols.io dx.doi.org/10.17504/protocols.io.cgvtw5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for the Gibson Assembly using the Gibson Assembly® Cloning Kit (E5510).
Proper citation: New England Biolabs 2015. Gibson Assembly® Protocol (E5510). protocols.io dx.doi.org/10.17504/protocols.io.cdms45 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Reaction Mixture for M0371. protocols.io dx.doi.org/10.17504/protocols.io.cgutwv Copy
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