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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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U Mass - Cytokines Panel I - multiplex Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.xvnfn5e | Mouse Metabolic Phenotyping Centers | Summary: This experiment provides the quantification of multiple cytokines and chemokines using multiplexed-Luminex technology based on beads containing specific antibodies. Serum cytokine levels reflect chronic or acute inflammation, and circulating cytokines and chemokines are altered in obesity. Cytokines Panel I include IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, KC (IL-8 homologue), IL-9, IL-10 (interleukin-10), IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, TNFα (tumor necrosis factor alpha), IFNγ (interferon gamma), IP-10 (interferon gamma-induced protein 10; CXCL-10), Eotaxin (CCL-11), G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte macrophage colony stimulating factor), LIF (leukemia inhibitory factor), LIX (LPS- induced CXC chemokine), MCP-1 (monocyte chemotactic protein-1; CCL-2), M-CSF (macrophage colony stimulating factor), MIG (monokine induced by gamma interferon; CXCL- 9), MIP-1α (macrophage inflammatory protein alpha; CCL-3), MIP-1 (macrophage inflammatory protein beta; CCL-4), and MIP-2/RANTES (macrophage inflammatory protein 2- alpha; CXCL-2). A service can be requested for all or any combination of listed cytokines/chemokines for customized multiplexed Luminex assay. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=190&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Cytokines Panel I - multiplex. protocols.io dx.doi.org/10.17504/protocols.io.xvnfn5e | 2021-03-29 03:12:35 | ||
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U Mass - Basal glucose metabolism Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.xr8fm9w | Mouse Metabolic Phenotyping Centers | Summary: Whole body glucose turnover and hepatic glucose production rates are measured at basal state using an intravenous infusion of labeled glucose in awake mice. Whole body glucose turnover and hepatic glucose production regulate basal glucose levels and are altered in obesity. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=137&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Basal glucose metabolism. protocols.io dx.doi.org/10.17504/protocols.io.xr8fm9w | 2021-03-29 03:12:08 | ||
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U Mass - Protein metabolism Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.x4ffqtn | Mouse Metabolic Phenotyping Centers | Hyperinsulinemic-euglycemic clamp is the gold-standard method to assess insulin sensitivity. The hyperinsulinemic-euglycemic clamp is widely used in clinics and laboratories to measure insulin action on glucose utilization in humans and animals for clinical and basic science research. Incorporation of radioactive-labeled glucose during hyperinsulinemic-euglycemic clamps makes it possible to measure glucose metabolism in individual organs in awake mice. Impaired insulin sensitivity (insulin resistance) is a major characteristic of obesity and an early requisite event in the development of type 2 diabetes. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=146&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Protein metabolism. protocols.io dx.doi.org/10.17504/protocols.io.x4ffqtn | 2021-03-29 03:09:57 | ||
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UC Davis - Intraperitoneal Insulin Tolerance Test Resource Report Resource Website |
Fawaz G. Haj | 10.17504/protocols.io.yp5fvq6 | Mouse Metabolic Phenotyping Centers | Summary: An intraperitoneal insulin tolerance test or ipITT is designed to determine the sensitivity of insulin-responsive tissues in the rodent. This is determined by measurement of glucose remaining in the circulation over time after a bolus ip insulin injection. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=84&docType=Protocol | 1 | 2019 | Fawaz G. Haj 2019. UC Davis - Intraperitoneal Insulin Tolerance Test. protocols.io dx.doi.org/10.17504/protocols.io.yp5fvq6 | 2021-03-29 03:10:02 | ||
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U Mass - Chronic high-fat feeding Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.xugfntw | Mouse Metabolic Phenotyping Centers | A high-fat diet of varying composition and percent fat is administered to induce obesity in mice. High-fat diet induced obesity is causally associated with insulin resistance and type 2 diabetes. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=150&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Chronic high-fat feeding. protocols.io dx.doi.org/10.17504/protocols.io.xugfntw | 2021-03-29 03:10:17 | ||
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UC Davis - Non-Esterified Fatty Acids Protocol Resource Report Resource Website |
Peter Havel | 10.17504/protocols.io.yutfwwn | Mouse Metabolic Phenotyping Centers | Summary: The Wako enzymatic method relies upon the acylation of coenzyme A (CoA) by the fatty acids in the presence of added acyl-CoA synthetase (ACS). The acyl-CoA thus produced is oxidized by added acyl- CoA oxidase (ACOD) with generation of hydrogen peroxide, in the presence of peroxidase (POD) permits the oxidative condensation of 3-methy-N-ethyl-N(β-hydroxyethyl)-aniline (MEFA) with 4-aminoantipyrine to form a purple colored adduct which can be measured colorimetrically at 550 nm. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=95&docType=Protocol | 1 | 2019 | Peter Havel 2019. UC Davis - Non-Esterified Fatty Acids Protocol. protocols.io dx.doi.org/10.17504/protocols.io.yutfwwn | 2021-03-29 03:10:16 | ||
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U Mass - Bilirubin Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.xscfnaw | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of bilirubin reflect bile acid metabolism. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=182&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Bilirubin. protocols.io dx.doi.org/10.17504/protocols.io.xscfnaw | 2021-03-29 03:10:18 | ||
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U Mass - Body composition (whole body) Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.xsqfndw | Mouse Metabolic Phenotyping Centers | The EchoMRI 3-in-1 uses ¹H- magnetic resonance spectroscopy to noninvasively measure whole body fat mass, lean mass, and water mass in awake mice. Whole body fat mass is a measure of adiposity that is altered in obesity. Whole body lean mass may be altered by exercise and in mouse models of altered metabolism. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=147&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Body composition (whole body). protocols.io dx.doi.org/10.17504/protocols.io.xsqfndw | 2021-03-29 03:10:24 | ||
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UC Davis - Alanine transaminase Resource Report Resource Website |
Peter Havel | 10.17504/protocols.io.ybtfsnn | Mouse Metabolic Phenotyping Centers | Summary: Alanine transaminase (ALT) also known as alanine aminotransferase or (sGPT) is a metabolic enzyme expressed primarily in the liver. Elevation of ALT levels is an indication of liver damage and has been associated with liver injury. ALT levels are monitored routinely in patients with liver diseases. ALT is also a very useful tool for preclinical investigation of experimental drug formulations and ALT levels are commonly used to monitor and attenuate the hepatotoxic effects of experimental drugs in rodents. | University of California, Davis, Metabolism and Endocrinology Core | https://mmpc.org/shared/document.aspx?id=289&docType=Protocol | 1 | 2019 | Peter Havel 2019. UC Davis - Alanine transaminase. protocols.io dx.doi.org/10.17504/protocols.io.ybtfsnn | 2021-03-29 03:10:20 | ||
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U Cinn - Non-invasive Measurement of Intestinal Fat Absorption Resource Report Resource Website |
Patrick Tso, Dana Lee | 10.17504/protocols.io.xmtfk6n | Mouse Metabolic Phenotyping Centers | Dietary fat containing 5% sucrose polybehenate, a non-absorbable food additive, is fed in a semi-synthetic diet containing absorbable fat to rodents. The animals eat the diet ad lib for 3 days. Two or more fecal pellets are collected on days 3 and 4. Fat absorption is calculated from the ratios of behenic acid to other fatty acids in the diet and in the feces as analyzed by gas chromatography of fatty acid methyl esters. | University of Cincinnati, University of Cincinnati | https://mmpc.org/shared/document.aspx?id=197&docType=Protocol | 1 | 2019 | Patrick Tso, Dana Lee 2019. U Cinn - Non-invasive Measurement of Intestinal Fat Absorption. protocols.io dx.doi.org/10.17504/protocols.io.xmtfk6n | 2021-03-29 03:10:25 | ||
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U Mass - Energy balance – food intake, energy expenditure, physical activity Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.xwwfpfe | Mouse Metabolic Phenotyping Centers | Summary:The TSE PhenoMaster/LabMaster Metabolic Cage system is used to measure indirect calorimetry, food/water intake, energy expenditure, and physical activity in individually housed awake mice. The experiment noninvasively measures VO² consumption and VCO² production rates in individual mice using metabolic chambers and calculates the respiratory exchange ratio (respiratory quotient) to reflect energy expenditure. Metabolic cage measurement is conducted continuously for 72 hours (3 days) to account for acclimation of mice housed in home cages during the study. With our high-speed Siemens O²/CO² sensing unit, indirect calorimetry measurements may be performed at a 20-min interval for a full 12-cage study. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=149&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Energy balance – food intake, energy expenditure, physical activity. protocols.io dx.doi.org/10.17504/protocols.io.xwwfpfe | 2021-03-29 03:10:20 | ||
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U Michigan - Retinal Microstructure Imaging OCT Resource Report Resource Website |
David A. Antonetti | 10.17504/protocols.io.yabfsan | Mouse Metabolic Phenotyping Centers | A spectral domain ophthalmic imaging system based on the technology of optical coherence tomography (OCT) will be used to acquire the microstructure image of retina in live animals. Like the ultrasound, but uses light waves instead of sound waves, the light backscattered from within a sample is processed to develop a high-resolution, depth-resolved image suitable for analyzing internal microstructure in vivo. | University of Michigan - Ann Arbor | https://mmpc.org/shared/document.aspx?id=308&docType=Protocol | 1 | 2019 | David A. Antonetti 2019. U Michigan - Retinal Microstructure Imaging OCT. protocols.io dx.doi.org/10.17504/protocols.io.yabfsan | 2021-03-29 03:10:33 | ||
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UC Davis - Metabolomics: Lipidomics analysis Resource Report Resource Website |
Oliver Fiehn | 10.17504/protocols.io.ys7fwhn | Mouse Metabolic Phenotyping Centers, Metabolomics Protocols & Workflows | Summary:Lipidomic analysis by UPLC-QTOF mass spectrometry | University of California, Davis | https://mmpc.org/shared/document.aspx?id=120&docType=Protocol | 1 | 2019 | Oliver Fiehn 2019. UC Davis - Metabolomics: Lipidomics analysis. protocols.io dx.doi.org/10.17504/protocols.io.ys7fwhn | 2021-03-29 03:10:28 | ||
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U Mass - Surgery – carotid artery cannulation Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.x4pfqvn | Mouse Metabolic Phenotyping Centers | Chronic indwelling catheter is placed in the carotid artery for blood sampling during experiments. Surival surgery is performed in anesthetized mice, and mice recover from surgery after 4~5 days. | University of Massachusetts | http://mmpc.org/shared/document.aspx?id=158&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Surgery – carotid artery cannulation. protocols.io dx.doi.org/10.17504/protocols.io.x4pfqvn | 2021-03-29 03:11:58 | ||
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U Michigan - Tail Cuff Blood Pressure Determination Resource Report Resource Website |
Jeff Hodgin | 10.17504/protocols.io.56eg9be | Mouse Metabolic Phenotyping Centers | This protocol provides information for the non-invasive measurement of blood pressure--tail cuff blood pressure determination operated by CODA tail-cuff blood pressure system in mice. | University of Michigan - Ann Arbor | https://mmpc.org/shared/document.aspx?id=315&docType=Protocol | 2 | 2019 | Jeff Hodgin 2019. U Michigan - Tail Cuff Blood Pressure Determination. protocols.io dx.doi.org/10.17504/protocols.io.56eg9be | 2021-03-29 03:12:02 | ||
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U Michigan - Nerve Conduction Velocity Resource Report Resource Website |
Eva Feldman | 10.17504/protocols.io.x96fr9e | Mouse Metabolic Phenotyping Centers | To confirm the presence of diabetic neuropathy, nerve conduction velocity (NCV) studies are performed. The animals are anesthetized with isoflurane to prevent discomfort. Body temperature is monitered with a dermal temperature probe and maintained at 32ºC with a warming lamp during NCV. Body temperature is maintained at 37ºC after NCV using a warming pad to ease animal stress from anesthetic. The nerve studies last less than 30 min per rat or mouse. The electrodes are cleaned with 70% alcohol between animals to maintain pathogen-free status. | University of Michigan - Ann Arbor | https://mmpc.org/shared/document.aspx?id=320&docType=Protocol | 1 | 2019 | Eva Feldman 2019. U Michigan - Nerve Conduction Velocity. protocols.io dx.doi.org/10.17504/protocols.io.x96fr9e | 2021-03-29 03:12:02 | ||
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UC Davis - HbA1c Protocol Resource Report Resource Website |
Peter Havel | 10.17504/protocols.io.ynrfvd6 | Mouse Metabolic Phenotyping Centers | Direct Enzymatic HbA1c test is an enzymatic assay in which lysed whole blood samples are subjected to extensive protease digestion with Bacillus sp protease. This process releases amino acids including glycated valines from the hemoglobin beta chains. Glycated valines then serve as substrates for specific recombinant fructosyl valine oxidase (FVO) enzyme, produced in E. coli. The recombinant FVO specifically cleaves N-terminal valines and produces hydrogen peroxide. This, in turn, is measured using a horseradish per-oxidase (POD) catalyzed reaction and a suitable chromagen. The HbA1c concentration is expressed directly as %HbA1c by use of a suit-able calibration curve in which the calibrators have values for each level in %HbA1c. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=89&docType=Protocol | 1 | 2019 | Peter Havel 2019. UC Davis - HbA1c Protocol. protocols.io dx.doi.org/10.17504/protocols.io.ynrfvd6 | 2021-03-29 03:12:04 | ||
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UC Davis - Massons Trichrome Resource Report Resource Website |
Jennifer Rutkowsky | 10.17504/protocols.io.56mg9c6 | Mouse Metabolic Phenotyping Centers | Massons trichrome staining is used for detection of collagen fibers in tissues such as skin, heart, etc. on formalin-fixed, paraffin-embedded sections, and may be used for frozen sections as well. The collagen fibers will be stained blue and the nuclei will be stained black and the background is stained red.Modified from: Massons Trichrome UC Davis Clinical Pathology lab | University of California, Davis | https://mmpc.org/shared/document.aspx?id=259&docType=Protocol | 2 | 2019 | Jennifer Rutkowsky 2019. UC Davis - Massons Trichrome. protocols.io dx.doi.org/10.17504/protocols.io.56mg9c6 | 2021-03-29 03:12:06 | ||
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Yale - Alanine Aminotransferase Resource Report Resource Website |
Gary Cline, John Stack | 10.17504/protocols.io.yz4fx8w | Mouse Metabolic Phenotyping Centers | Procedure used to measure the Alanine Amino activity in blood, plasma, and serum. Alanine Amino (ALT) activity is measured by the enzymatically coupled reactions of ALT (to form pyruvate from alanine and α-ketoglutarate) and Lactate dehydrogenase (conversion of pyruvate to lactate with oxdiation of NADH to NAD). The rate of NAD formation is monitored by the change in absorbance at 340 nm. | Yale University, Yale University | https://mmpc.org/shared/document.aspx?id=206&docType=Protocol | 1 | 2019 | Gary Cline, John Stack 2019. Yale - Alanine Aminotransferase. protocols.io dx.doi.org/10.17504/protocols.io.yz4fx8w | 2021-03-29 03:12:05 | ||
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UC Davis - Hematoxylin and Eosin (H&E) Resource Report Resource Website |
Jennifer Rutkowsky | 10.17504/protocols.io.56sg9ee | Mouse Metabolic Phenotyping Centers | Staining tissues on a slide with Heatoxylin and Eosin.Modified from: H &E protocol, UC Davis Clinical Pathology lab | University of California, Davis | https://mmpc.org/shared/document.aspx?id=255&docType=Protocol | 2 | 2019 | Jennifer Rutkowsky 2019. UC Davis - Hematoxylin and Eosin (H&E). protocols.io dx.doi.org/10.17504/protocols.io.56sg9ee | 2021-03-29 03:08:13 |
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