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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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Quantifying Biogenic Silica (bSi) Deposition Rates Adapted Method Resource Report Resource Website |
Brittany Zepernick, Matthew Saxton, Steven Wilhelm | 10.17504/protocols.io.72vhqe6 | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Great Lakes Center for Fresh Waters and Human Health | This method can be used to assess and quantify the rate of silica deposition (bSi) over time in diatoms to determine their rate of frustule synthesis. This protocol has been adapted for the processing of both cultures as well as environmental samples when inoculated with PDMPO [2‐(4‐pyridyl)‐5‐((4‐(2dimethylaminoethylaminocarbamoyl)methoxy)phenyl)oxazole], which is a fluorescent dye that is co-deposited with silica during frustule biosynthesis in a 3230:1 Si:PDMPO (mol:mol) ratio. | University of Tennessee, Knoxville, Miami University of Ohio, The University of Tennessee, Knoxville | http://wilhelmlab.utk.edu/ | 1 | 2019 | Brittany Zepernick, Matthew Saxton, Steven Wilhelm 2019. Quantifying Biogenic Silica (bSi) Deposition Rates Adapted Method. protocols.io dx.doi.org/10.17504/protocols.io.72vhqe6 | 2021-03-29 03:13:06 | ||
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Chlorella Virus Plaque Assay Resource Report Resource Website |
Steven Wilhelm | 10.17504/protocols.io.ge2btge | VERVE Net, Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Adapted from: Van Etten, J. (n.d.). Titering of Chlorella Viruses. Retrieved from http://ncv.unl.edu/vanettenlab/ | The University of Tennessee, Knoxville | http://ncv.unl.edu/vanettenlab/Plaque%20assay.pdf | 1 | 2016 | Steven Wilhelm 2016. Chlorella Virus Plaque Assay. protocols.io dx.doi.org/10.17504/protocols.io.ge2btge | 2021-03-29 03:13:16 | ||
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Phenol/chloroform extraction of DNA from cyanobacteria Resource Report Resource Website |
Robbie Martin, Steven W. Wilhelm | 10.17504/protocols.io.ptndnme | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Phenol/chloroform-based DNA extraction from cells pre-treated with RNase A, lysozyme, proteinase K, and SDS. The protocol was optimized for extracting DNA from Microcystis aeruginosa, but works well for other cyanobacteria. | University of Tennessee, Knoxville, University of Tennessee, Knoxville | 2 | 2018 | Robbie Martin, Steven W. Wilhelm 2018. Phenol/chloroform extraction of DNA from cyanobacteria. protocols.io dx.doi.org/10.17504/protocols.io.ptndnme | 2021-03-29 03:13:27 | |||
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Safety protocols for Aquatic Microbial Ecology Research Group, Department of Microbiology, The University of Tennessee. Resource Report Resource Website |
Steven Wilhelm, Gary Lecleir, Ashley Humphrey | 10.17504/protocols.io.6mmhc46 | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | This is a collection of safety protocols as used by the Aquatic Microbial Ecology Research Group. Science & Engineering Room 637 (O); 634 (Lab) | The University of Tennessee, Knoxville, The University of Tennessee, Knoxville, University of Tennessee, Knoxville | 2 | 2019 | Steven Wilhelm, Gary Lecleir, Ashley Humphrey 2019. Safety protocols for Aquatic Microbial Ecology Research Group, Department of Microbiology, The University of Tennessee.. protocols.io dx.doi.org/10.17504/protocols.io.6mmhc46 | 2021-03-29 03:13:25 | |||
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BG-11 Media Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.h9zb976 | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from the original publication Rippka, R., DeRulles, J., Waterbury, J. B., Herdman, M. & Stanier, R. Y. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111, 1-61 (1979). | 3 | 2017 | Dr. Steven Wilhelm 2017. BG-11 Media. protocols.io dx.doi.org/10.17504/protocols.io.h9zb976 | 2021-03-29 03:13:33 | ||||
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Arnow Test for Catecholate-Type Siderophores Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.icpcavn | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from Arnow, L. E. Colorimetric determination of components of 3,4-dihydroxyphenylalanine-tyrosine mixtures. J Biol Chem 118, 531-537 (1937). | 1 | 2017 | Dr. Steven Wilhelm 2017. Arnow Test for Catecholate-Type Siderophores. protocols.io dx.doi.org/10.17504/protocols.io.icpcavn | 2021-03-29 03:13:41 | ||||
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Safety protocols for Aquatic Microbial Ecology Research Group, Department of Microbiology, The University of Tennessee. Resource Report Resource Website |
Steven Wilhelm, Gary Lecleir, Ashley Humphrey | 10.17504/protocols.io.wggfbtw | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | This is a collection of safety protocols as used by the Aquatic Microbial Ecology Research Group. Science & Engineering Room 637 (O); 634 (Lab) | The University of Tennessee, Knoxville, The University of Tennessee, Knoxville, University of Tennessee, Knoxville | 1 | 2019 | Steven Wilhelm, Gary Lecleir, Ashley Humphrey 2019. Safety protocols for Aquatic Microbial Ecology Research Group, Department of Microbiology, The University of Tennessee.. protocols.io dx.doi.org/10.17504/protocols.io.wggfbtw | 2021-03-29 03:14:32 | |||
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MBBM Media Resource Report Resource Website |
Alyssa Alsante | 10.17504/protocols.io.gdrbs56 | Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Van Etten, J. (n.d.). Formulation of Modified Bold's Basal Medium (MBBM). Retrieved from http://ncv.unl.edu/vanettenlab/ | http://ncv.unl.edu/vanettenlab/MBBM.pdf | 2 | 2016 | Alyssa Alsante 2016. MBBM Media. protocols.io dx.doi.org/10.17504/protocols.io.gdrbs56 | 2021-03-29 03:14:43 | |||
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M9 Minimal Media Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.ibqcamw | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocolModified from Pardee, A. B., F. Jacob, and J. Monod. 1959. The genetic control and cytoplasmic expression of "inducibility" in the synthesis of ß-galactosidase in E. coli. J. Mol. Biol. 1:165-178 | 1 | 2017 | Dr. Steven Wilhelm 2017. M9 Minimal Media. protocols.io dx.doi.org/10.17504/protocols.io.ibqcamw | 2021-03-29 03:15:20 | ||||
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ASP12A Recipe for culturing Aureococcus anophagefferens Resource Report Resource Website |
Eric Gann | 10.17504/protocols.io.f3ybqpw | Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Defined Medium for Aureococcus anophagefferens | http://www.nefsc.noaa.gov/nefsc/publications/crd/crd0517/crd0517.pdf | 2 | 2016 | Eric Gann 2016. ASP12A Recipe for culturing Aureococcus anophagefferens. protocols.io dx.doi.org/10.17504/protocols.io.f3ybqpw | 2021-03-29 03:12:07 | |||
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Ti (III) Reagent Resource Report Resource Website |
Steven Wilhelm | 10.17504/protocols.io.iisccee | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Used for washing off extracellular iron.Please contact Dr. Steven Wilhelm ([email protected]) for any additional information regarding this protocol. | The University of Tennessee, Knoxville | 1 | 2019 | Steven Wilhelm 2019. Ti (III) Reagent . protocols.io dx.doi.org/10.17504/protocols.io.iisccee | 2021-03-29 03:12:07 | |||
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Epifluorescent Microscopy of Virus Particles Using SYBR Green Resource Report Resource Website |
Steven Wilhelm | 10.17504/protocols.io.gssbwee | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Adapted from: Ortmann and Suttle (2009) Determination of Virus Abundance by Epifluorescence microscopy. Ch. 10 Methods of Molecular Biology. | The University of Tennessee, Knoxville | 1 | 2017 | Steven Wilhelm 2017. Epifluorescent Microscopy of Virus Particles Using SYBR Green. protocols.io dx.doi.org/10.17504/protocols.io.gssbwee | 2021-03-29 03:12:13 |
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