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Authors: Brittany Zepernick, Matthew Saxton, Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Great Lakes Center for Fresh Waters and Human Health
Summary: This method can be used to assess and quantify the rate of silica deposition (bSi) over time in diatoms to determine their rate of frustule synthesis. This protocol has been adapted for the processing of both cultures as well as environmental samples when inoculated with PDMPO [2‐(4‐pyridyl)‐5‐((4‐(2dimethylaminoethylaminocarbamoyl)methoxy)phenyl)oxazole], which is a fluorescent dye that is co-deposited with silica during frustule biosynthesis in a 3230:1 Si:PDMPO (mol:mol) ratio.
Proper citation: Brittany Zepernick, Matthew Saxton, Steven Wilhelm 2019. Quantifying Biogenic Silica (bSi) Deposition Rates Adapted Method. protocols.io dx.doi.org/10.17504/protocols.io.72vhqe6 Copy
Authors: Steven Wilhelm
Group: VERVE Net, Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Adapted from: Van Etten, J. (n.d.). Titering of Chlorella Viruses. Retrieved from http://ncv.unl.edu/vanettenlab/
Proper citation: Steven Wilhelm 2016. Chlorella Virus Plaque Assay. protocols.io dx.doi.org/10.17504/protocols.io.ge2btge Copy
Authors: Robbie Martin, Steven W. Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Phenol/chloroform-based DNA extraction from cells pre-treated with RNase A, lysozyme, proteinase K, and SDS. The protocol was optimized for extracting DNA from Microcystis aeruginosa, but works well for other cyanobacteria.
Proper citation: Robbie Martin, Steven W. Wilhelm 2018. Phenol/chloroform extraction of DNA from cyanobacteria. protocols.io dx.doi.org/10.17504/protocols.io.ptndnme Copy
Authors: Steven Wilhelm, Gary Lecleir, Ashley Humphrey
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: This is a collection of safety protocols as used by the Aquatic Microbial Ecology Research Group. Science & Engineering Room 637 (O); 634 (Lab)
Proper citation: Steven Wilhelm, Gary Lecleir, Ashley Humphrey 2019. Safety protocols for Aquatic Microbial Ecology Research Group, Department of Microbiology, The University of Tennessee.. protocols.io dx.doi.org/10.17504/protocols.io.6mmhc46 Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from the original publication Rippka, R., DeRulles, J., Waterbury, J. B., Herdman, M. & Stanier, R. Y. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111, 1-61 (1979).
Proper citation: Dr. Steven Wilhelm 2017. BG-11 Media. protocols.io dx.doi.org/10.17504/protocols.io.h9zb976 Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from Arnow, L. E. Colorimetric determination of components of 3,4-dihydroxyphenylalanine-tyrosine mixtures. J Biol Chem 118, 531-537 (1937).
Proper citation: Dr. Steven Wilhelm 2017. Arnow Test for Catecholate-Type Siderophores. protocols.io dx.doi.org/10.17504/protocols.io.icpcavn Copy
Authors: Steven Wilhelm, Gary Lecleir, Ashley Humphrey
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: This is a collection of safety protocols as used by the Aquatic Microbial Ecology Research Group. Science & Engineering Room 637 (O); 634 (Lab)
Proper citation: Steven Wilhelm, Gary Lecleir, Ashley Humphrey 2019. Safety protocols for Aquatic Microbial Ecology Research Group, Department of Microbiology, The University of Tennessee.. protocols.io dx.doi.org/10.17504/protocols.io.wggfbtw Copy
Authors: Alyssa Alsante
Group: Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Van Etten, J. (n.d.). Formulation of Modified Bold's Basal Medium (MBBM). Retrieved from http://ncv.unl.edu/vanettenlab/
Proper citation: Alyssa Alsante 2016. MBBM Media. protocols.io dx.doi.org/10.17504/protocols.io.gdrbs56 Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocolModified from Pardee, A. B., F. Jacob, and J. Monod. 1959. The genetic control and cytoplasmic expression of "inducibility" in the synthesis of ß-galactosidase in E. coli. J. Mol. Biol. 1:165-178
Proper citation: Dr. Steven Wilhelm 2017. M9 Minimal Media. protocols.io dx.doi.org/10.17504/protocols.io.ibqcamw Copy
Authors: Eric Gann
Group: Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Defined Medium for Aureococcus anophagefferens
Proper citation: Eric Gann 2016. ASP12A Recipe for culturing Aureococcus anophagefferens. protocols.io dx.doi.org/10.17504/protocols.io.f3ybqpw Copy
Authors: Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Used for washing off extracellular iron.Please contact Dr. Steven Wilhelm ([email protected]) for any additional information regarding this protocol.
Proper citation: Steven Wilhelm 2019. Ti (III) Reagent . protocols.io dx.doi.org/10.17504/protocols.io.iisccee Copy
Authors: Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Adapted from: Ortmann and Suttle (2009) Determination of Virus Abundance by Epifluorescence microscopy. Ch. 10 Methods of Molecular Biology.
Proper citation: Steven Wilhelm 2017. Epifluorescent Microscopy of Virus Particles Using SYBR Green. protocols.io dx.doi.org/10.17504/protocols.io.gssbwee Copy
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