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| Name | Authors | DOI | Group |
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RRIDs used | ||||||
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FCMPASS - Cataloguing fluorescence reference materials Resource Report Resource Website |
Joshua Welsh, Jennifer Jones | 10.17504/protocols.io.bhvvj666 | Translational Nanobiology Section | This protocol outlines the steps required to catalogue fluorescence reference materials using the FCMPASS software. This is one of a number of protocols in the pipeline for performing small particle calibration using the fcmpass software package. | Translataional Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Translataional Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health | 1 | 2020 | Joshua Welsh, Jennifer Jones 2020. FCMPASS - Cataloguing fluorescence reference materials. protocols.io dx.doi.org/10.17504/protocols.io.bhvvj666 | 2021-03-29 03:08:47 | |||
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Protoplast Isolation - Incubation Buffer Resource Report Resource Website |
Steven Burgess | 10.17504/protocols.io.fd3bi8n | OpenPlant Project | Instructions to make 50 mL of incubation buffer for protoplast isolation.Adapted from Yoo et al. 2007 http://www.nature.com/nprot/journal/v2/n7/full/nprot.2007.199.html | Yu Z, Boehm CR, Hibberd JM, Abell C, Haseloff J, Burgess SJ, Reyna-Llorens I, Droplet-based microfluidic analysis and screening of single plant cells. PLoS ONE 13(5). doi: 10.1371/journal.pone.0196810 | University of Cambridge | https://doi.org/10.1371/journal.pone.0196810 | 1 | 2016 | Steven Burgess 2016. Protoplast Isolation - Incubation Buffer. protocols.io dx.doi.org/10.17504/protocols.io.fd3bi8n | 2021-03-29 03:08:46 | |
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Heterologous protein expression in E. coli Resource Report Resource Website |
Diep Ganguly, Timothy Rhodes, Nay Chi Khin, Estee E Tee, Kai Xun Chan | 10.17504/protocols.io.95qh85w | Pogson Genomics Group | Protocol for recombinant protein expression in E. coli for protein purification and subsequent enzyme assays, protein crystallography etc. | The Australian National University, The Australian National University, The Australian National University, The Australian National University, The Australian National University | 4 | 2019 | Diep Ganguly, Timothy Rhodes, Nay Chi Khin, Estee E Tee, Kai Xun Chan 2019. Heterologous protein expression in E. coli. protocols.io dx.doi.org/10.17504/protocols.io.95qh85w | 2021-03-29 03:08:46 | |||
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Transfected Naegleria Fluorescence Microscopy Resource Report Resource Website |
Anastasios Tsaousis | 10.17504/protocols.io.hpvb5n6 | Protist Research to Optimize Tools in Genetics (PROT-G) | University of Kent | 1 | 2017 | Anastasios Tsaousis 2017. Transfected Naegleria Fluorescence Microscopy. protocols.io dx.doi.org/10.17504/protocols.io.hpvb5n6 | 2021-03-29 03:08:46 | ||||
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High-Yield Skeletal Muscle Protein Recovery from TRIzol® after RNA and DNA Extraction Resource Report Resource Website |
Yuan Wen, Ivan Vechetti , Taylor Valentino, John J McCarthy | 10.17504/protocols.io.bii3kcgn | Extraction of DNA, RNA, and protein from the same sample would allow for direct comparison of genomic, transcriptomic, and proteomic information. Commercially available kits exhibit poor protein yield and TRIzol® reagent produces a protein pellet that is extremely difficult to solubilize. In response to these limitations, this study presents an optimized method for the extraction of protein from the organic phase of TRIzol® reagent that allows for higher yield recovery of skeletal muscle protein compared to direct homogenization in a common protein lysis buffer. The presented method is inexpensive, simple and fast; requires no additional treatment of the protein pellet for dissolution; and is compatible with downstream western blot applications. | University of Kentucky, University of Kentucky, University of Kentucky, University of Kentucky | 1 | 2020 | Yuan Wen, Ivan Vechetti , Taylor Valentino, John J McCarthy 2020. High-Yield Skeletal Muscle Protein Recovery from TRIzol® after RNA and DNA Extraction. protocols.io dx.doi.org/10.17504/protocols.io.bii3kcgn | 2021-03-29 03:08:48 | ||||
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0.9% Saline Solution Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bg44jyyw | BICCN, Allen Institute for Brain Science | This protocol is used to prepare 0.9% Saline Solution. 0.9% Sterile Saline solution is used as an initial rinse for the brains of mice prior to perfusion. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. 0.9% Saline Solution. protocols.io dx.doi.org/10.17504/protocols.io.bg44jyyw | 2021-03-29 03:08:48 | |||
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Bioelectrochemistry protocol For CHI Potentiostat Resource Report Resource Website |
Rose Jones, Beth Orcutt | 10.17504/protocols.io.xihfkb6 | Orcutt Deep Biosphere Lab | The following protocol describes how to prepare microbial fuel cells (MFCs) with Indium Tin Oxide (ITO) electrodes for use as a poised potential enrichment tool, specifically an oxic system to enrich for the cathode-oxidizing community. The protocol describes setting up four “online” MFCs that are connected to a potentiostat at a particular set voltage plus one “offline” unconnected control (to account for non-electrode related changes). One of the online MFCs is a “negative” killed substrate control (to account for abiotic medium-source current fluctuations) and the remaining three MFCs being replicate live samples. This protocol is based on advice primarily from Frauke Kracke and Annette Rowe. | Bigelow Laboratory of Ocean Science, Bigelow Laboratory for Ocean Sciences | 1 | 2019 | Rose Jones, Beth Orcutt 2019. Bioelectrochemistry protocol For CHI Potentiostat. protocols.io dx.doi.org/10.17504/protocols.io.xihfkb6 | 2021-03-29 03:08:48 | |||
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MojoSort™ Streptavidin Nanobeads Protocol Resource Report Resource Website |
Kelsey Miller | 10.17504/protocols.io.e2rbgd6 | BioLegend | The cells targeted by the Streptavidin Nanobeads are either selected or depleted by incubating your sample with the magnetic particles after incubating with a biotin-conjugated antibody or antibody cocktail. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the targeted cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. | BioLegend | http://www.biolegend.com/media_assets/support_protocol/MojoSort_Streptavidin_Nanobeads_Protocol_V02_10192015.pdf | 1 | 2016 | Kelsey Miller 2016. MojoSort™ Streptavidin Nanobeads Protocol. protocols.io dx.doi.org/10.17504/protocols.io.e2rbgd6 | 2021-03-29 03:08:48 | ||
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Plant Infiltration for Mimulus in Planta Transformation Resource Report Resource Website |
Yaowu Yuan | 10.17504/protocols.io.3rtgm6n | Mimulus | This protocol is part of a collection for Mimulus in planta transformation. | University of Connecticut | http://mimubase.org/FTP/Protocols/Stable_Transformation/Mimulus%20in%20planta%20transformation.pdf | 1 | 2019 | Yaowu Yuan 2019. Plant Infiltration for Mimulus in Planta Transformation. protocols.io dx.doi.org/10.17504/protocols.io.3rtgm6n | 2021-03-29 03:08:46 | ||
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GFP ELISA Assay Protocol Resource Report Resource Website |
James P. O’Callaghan, PhD | 10.17504/protocols.io.hsub6ew | This is the protocol to be used in the quantification of glial fibrillary acidic protein (GFAP) in mouse brain tissue. | Kelly KA, Michalovicz LT, Miller JV, Castranova V, Miller DB, O’Callaghan JP, Prior exposure to corticosterone markedly enhances and prolongs the neuroinflammatory response to systemic challenge with LPS. PLoS ONE 13(1). doi: 10.1371/journal.pone.0190546 | , | https://doi.org/10.1371/journal.pone.0190546 | 3 | 2018 | James P. O’Callaghan, PhD 2018. GFP ELISA Assay Protocol. protocols.io dx.doi.org/10.17504/protocols.io.hsub6ew | 2021-03-29 03:08:46 | ||
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Sequencing library preparation Resource Report Resource Website |
Adriana Alberti, Julie Poulain, Stefan Engelen, Karine Labadie, Sarah Romac, Isabel Ferrera, Guillaume Albini, Jean-Marc Aury, Caroline Belser, Alexis Bertrand, Corinne Cruaud, Corinne Da Silva, Carole Dossat, Frédéric Gavory, Shahinaz Gas, Julie Guy, Maud Haquelle, E'krame Jacoby, Olivier Jaillon, Arnaud Lemainque, Eric Pelletier, Gaëlle Samson, Marc Wessner, Genoscope Technical Team, Silvia G. Acinas, Marta Royo-Llonch, Francisco M. Cornejo-Castillo, Ramiro Logares, Beatriz Fernández-Gómez, Chris Bowler, Guy Cochrane, Clara Amid, Petra Ten Hoopen, Colomban De Vargas, Nigel Grimsley, Elodie Desgranges, Stefanie Kandels-Lewis, Hiroyuki Ogata, Nicole Poulton, Michael E. Sieracki, Ramunas Stepanauskas, Matthew B. Sullivan, Jennifer R. Brum, Melissa B. Duhaime, Bonnie T. Poulos, Bonnie L. Hurwitz, Stéphane Pesant, Eric Karsenti, Patrick Wincker | 10.17504/protocols.io.qwidxce | Tara Oceans | This protocol describes the sequencing library preparation for the Tara Oceans expedition and is part of Viral to metazoan marine plankton nucleotide sequences from the Tara Oceans expedition. Figure 1: Overview of -omics analysis strategy applied on Tara Oceans samples. | CEA, Institut de Biologie Intégrative de la Cellule, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CNRS, UMR 7144, Station Biologique de Roscoff, France; Sorbonne Universités, UPMC Univ Paris 06, UMR 7144, Station Biologique de Roscoff, France, Departament de Biologia Marina i Oceanografia, Institute of Marine Sciences (ICM), CSIC, Barcelona, Spain, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France; CNRS, UMR 8030, Evry , France; Université d'Evry, UMR 8030, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France, Departament de Biologia Marina i Oceanografia, Institute of Marine Sciences (ICM), CSIC, Barcelona, Spain, Departament de Biologia Marina i Oceanografia, Institute of Marine Sciences (ICM), CSIC, Barcelona, Spain, Departament de Biologia Marina i Oceanografia, Institute of Marine Sciences (ICM), CSIC, Barcelona, Spain, Departament de Biologia Marina i Oceanografia, Institute of Marine Sciences (ICM), CSIC, Barcelona, Spain, Departament de Biologia Marina i Oceanografia, Institute of Marine Sciences (ICM), CSIC, Barcelona, Spain; FONDAP Center for Genome Regulation, Santiago, Chile; Laboratorio de Bioinformática y Expresión Génica, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, El Libano Macul, Santiago, Chile, Ecole Normale Supérieure, PSL Research University, Institut de Biologie de l’Ecole Normale Supérieure (IBENS), CNRS UMR 8197, INSERM U1024, Paris, France, European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genomes Campus, Hinxton, Cambridge , UK, European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genomes Campus, Hinxton, Cambridge , UK, European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genomes Campus, Hinxton, Cambridge , UK, CNRS, UMR 7144, Station Biologique de Roscoff, France; Sorbonne Universités, UPMC Univ Paris 06, UMR 7144, Station Biologique de Roscoff, France, CNRS UMR 7232, BIOM, Banyuls-sur-Mer, France; Sorbonne Universités Paris 06, OOB UPMC, Banyuls-sur-Mer , France, CNRS UMR 7232, BIOM, Banyuls-sur-Mer, France; Sorbonne Universités Paris 06, OOB UPMC, Banyuls-sur-Mer , France, Directors’ Research European Molecular Biology Laboratory, Heidelberg, Germany; Structural and Computational Biology, European Molecular Biology Laboratory, Heidelberg, Germany, Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto, Japan, Bigelow Laboratory for Ocean Sciences, East Boothbay, Maine, USA, Bigelow Laboratory for Ocean Sciences, East Boothbay, Maine, USA; National Science Foundation, Arlington, Virginia, USA, Bigelow Laboratory for Ocean Sciences, East Boothbay, Maine, USA, Departments of Microbiology and Civil, Environmental and Geodetic Engineering, Ohio State University, Columbus, Ohio, USA; Department of Microbiology, The Ohio State University, Columbus, Ohio, USA, Department of Microbiology, The Ohio State University, Columbus, Ohio, USA; Present address: Department of Oceanography and Coastal Sciences, Louisiana State University, Baton Rouge, Louisiana, USA, Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan, USA, University of Arizona, Tucson, Arizona, USA, Department of Agricultural and Biosystems Engineering, University of Arizona, Tucson, Arizona, USA, MARUM, Center for Marine Environmental Sciences, University of Bremen, Germany; PANGAEA, Data Publisher for Earth and Environmental Science, University of Bremen, Germany, Ecole Normale Supérieure, PSL Research University, Institut de Biologie de l’Ecole Normale Supérieure (IBENS), CNRS UMR 8197, INSERM U1024, Paris, France; Directors’ Research European Molecular Biology Laboratory, Heidelberg, Germany; Sorbonne Universités, UPMC Université Paris 06, CNRS, Laboratoire d’oceanographie de Villefranche (LOV), Observatoire Océanologique, Villefranche-sur-mer, France, CEA - Institut de Biologie François Jacob, Genoscope, Evry, France; CNRS, UMR 8030, Evry , France; Université d'Evry, UMR 8030, Evry, France | https://www.nature.com/articles/sdata201793#methods | 1 | 2020 | Adriana Alberti, Julie Poulain, Stefan Engelen, Karine Labadie, Sarah Romac, Isabel Ferrera, Guillaume Albini, Jean-Marc Aury, Caroline Belser, Alexis Bertrand, Corinne Cruaud, Corinne Da Silva, Carole Dossat, Frédéric Gavory, Shahinaz Gas, Julie Guy, Maud Haquelle, E'krame Jacoby, Olivier Jaillon, Arnaud Lemainque, Eric Pelletier, Gaëlle Samson, Marc Wessner, Genoscope Technical Team, Silvia G. Acinas, Marta Royo-Llonch, Francisco M. Cornejo-Castillo, Ramiro Logares, Beatriz Fernández-Gómez, Chris Bowler, Guy Cochrane, Clara Amid, Petra Ten Hoopen, Colomban De Vargas, Nigel Grimsley, Elodie Desgranges, Stefanie Kandels-Lewis, Hiroyuki Ogata, Nicole Poulton, Michael E. Sieracki, Ramunas Stepanauskas, Matthew B. Sullivan, Jennifer R. Brum, Melissa B. Duhaime, Bonnie T. Poulos, Bonnie L. Hurwitz, Stéphane Pesant, Eric Karsenti, Patrick Wincker 2020. Sequencing library preparation. protocols.io dx.doi.org/10.17504/protocols.io.qwidxce | 2021-03-29 03:08:46 | ||
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Tracking acute response of C elegans to cocaine Resource Report Resource Website |
Priota Islam | 10.17504/protocols.io.xvffn3n | Behavioural Genomics | Our aim is to study addiction in C elegans with cocaine being the drug choice of abuse. This short experiment is done to check how the worms react instantaneously upon exposure to this drug for the first time. | Imperial College London | 1 | 2019 | Priota Islam 2019. Tracking acute response of C elegans to cocaine. protocols.io dx.doi.org/10.17504/protocols.io.xvffn3n | 2021-03-29 03:08:47 | |||
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ChroDrip - ProteinG Resource Report Resource Website |
Alexandra Ehl, David Frommholz, Nadine Stefanczyk | 10.17504/protocols.io.uwbexan | Purification Guide for the Isolation of Antibodies with ChroDrip Columns by DALEX Biotech.Easy and quick small scale antibody purification from various sources and species.Each ChroDrip column has a binding capacity of > 15 mg/ml (tested with human polyclonal Ig, binding varies between species and clones).The proprietary resin does not shrink or swell in aqueous buffers.High pressure stability.pH stability short term 2 - 8, long term 3 - 8.Excellent thermal stability up to 15 minutes at 80 °C in aqueous buffers at neutral pH.Can be dried for long term storage (80 °C for > 2 h). | DALEX Biotech, DALEX Biotech, DALEX Biotech | https://dalex-biotech.com/ | 1 | 2018 | Alexandra Ehl, David Frommholz, Nadine Stefanczyk 2018. ChroDrip - ProteinG. protocols.io dx.doi.org/10.17504/protocols.io.uwbexan | 2021-03-29 03:08:47 | |||
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Double Digestion of Insert DNA Resource Report Resource Website |
Igem Dusseldorf | 10.17504/protocols.io.79ehr3e | Heinrich-Heine Universität Düsseldorf | 1 | 2019 | Igem Dusseldorf 2019. Double Digestion of Insert DNA. protocols.io dx.doi.org/10.17504/protocols.io.79ehr3e | 2021-03-29 03:08:47 | |||||
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Stranded Transcript Count Table Generation from Long Reads Resource Report Resource Website |
David Eccles | 10.17504/protocols.io.smuec6w | High molecular weight DNA extraction from all kingdoms | This protocol is for comparing two different samples at the transcript level, using long reads that are mapped to transcripts.Input(s): stranded fastq files (see steps 1-8 of Stranded Mapping from Long Reads), transcript reference fasta file, annotation fileOutput(s): transcript table, sorted by differential coverage, annotated with gene name / description / location | Malaghan Institute of Medical Research (NZ) | 1 | 2018 | David Eccles 2018. Stranded Transcript Count Table Generation from Long Reads. protocols.io dx.doi.org/10.17504/protocols.io.smuec6w | 2021-03-29 03:08:47 | |||
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Is Expired Air Carbon Monoxide Testing Effective for Screening of Cigarette Use in Orthopaedic Patients? Resource Report Resource Website |
Sean Sterrenberg, David Gallacher, Melissa Schmidt, Rebecca Rugel, Summer Gerke, Kenneth Gundle, Ryan Wallenberg | 10.17504/protocols.io.7crhiv6 | Smokerlyzer Study, PVAMC | Background: Tobacco use is associated with increased post-operative complications and is often underreported by patients. Traditional biochemical testing methods, including serum and urine cotinine assays, do not differentiate active smoking from nicotine replacement therapy (NRT). This study intends to evaluate the effectiveness of a novel point-of-care carbon monoxide (CO) breath test to verify smoking status and differentiate active smoking from NRT pre-operatively in a cohort of veteran orthopaedic patients. Methods: This is a single-institution, prospective cohort study of adult orthopaedic patients at the Portland VA Medical Center to implement and evaluate a point-of-care CO breath test, the Smokerlyzer® Micro EC50, for pre-operative smoking status verification. Patients who are indicated for orthopaedic surgery in the pre-operative clinic will be offered inclusion. Self-reported smoking status will be obtained pre-operatively to categorize patients as non-smoker, quitters, or active smokers. Exhaled CO and serum cotinine levels will be obtained at the initial clinic visit, pre-operative visit, and day of surgery. Primary endpoints are concordance between self-reported smoking status and CO levels, and concordance between exhaled CO and serum cotinine levels.Discussion: Pre-operative smoking cessation interventions have been shown to decrease smoking rates on the day of surgery and promote abstinence 30 days post-operatively. NRTs are commonly used to promote smoking cessation, however their effect on surgical outcomes are not known. This study aims to validate the use of point-of-care testing that differentiates active smoking from NRT, and identify patients at risk for smoking status misclassification. Results would provide preliminary data to directly test NRT use, separate from tobacco use, and surgical outcomes.Specific Aims: 1. To determine if expired air CO levels can be used to identify patients that are at risk of smoking status misclassification pre-operatively, including patients that are on nicotine replacement therapy. 2. To evaluate the concordance between self-reported smoking and expired air CO levels in the veteran orthopedic population. 3. To evaluate the concordance between pre-operative expired air CO and typical serum levels in the orthopedic population. Hypotheses: 1. Expired air CO testing will allow for differentiation between active smokers and those on nicotine replacement therapy compared to serum cotinine testing. 2. Self-reported “quitters” will have lower agreement between self-reported smoking status and biological testing compared to self-reported “non-smokers” and “active smokers.”3. Compared to serum cotinine testing, expired air CO testing will allow for non-inferior accuracy in verifying smoking status. | Oregon Health Sciences University, Oregon Health Sciences University, Portland Veterans Affairs Medical Center, Portland Veterans Affairs Medical Center, Portland Veterans Affairs Medical Center, Oregon Health Sciences University, Portland Veterans Affairs Medical Center | https://www.bedfont.com/shop/smokerlyzer/micro | 1 | 2019 | Sean Sterrenberg, David Gallacher, Melissa Schmidt, Rebecca Rugel, Summer Gerke, Kenneth Gundle, Ryan Wallenberg 2019. Is Expired Air Carbon Monoxide Testing Effective for Screening of Cigarette Use in Orthopaedic Patients?. protocols.io dx.doi.org/10.17504/protocols.io.7crhiv6 | 2021-03-29 03:08:47 | ||
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Collection of skin biopsies from cattle Resource Report Resource Website |
Froylan Sosa, Peter Hansen | 10.17504/protocols.io.bm3yk8pw | This is a simple protocol for collecting skin biopsies from cattle. The procedure described here was used to collect skin for histological analysis but it could also be used to collect skin for PCR or other purposes. Our experience indicates that there are few complications with the procedure. The most common is that occasionally there is a slight amount of bleeding that can be treated by applying pressure using a gauze. Healing occurs rapidly and biopsy sites are difficult to detect 14 days after the procedure. The protocol is best read by using the attached pdf file. | University of Florida, University of Florida | 1 | 2020 | Froylan Sosa, Peter Hansen 2020. Collection of skin biopsies from cattle. protocols.io dx.doi.org/10.17504/protocols.io.bm3yk8pw | 2021-03-29 03:08:47 | ||||
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Holo-ZitRMG binding to dsDNA fragments by ITC Resource Report Resource Website |
Paloma Varela | 10.17504/protocols.io.vg5e3y6 | Varela PF, Velours C, Aumont-Niçaise M, Pineau B, Legrand P, Poquet I (2019) Biophysical and structural characterization of a zinc-responsive repressor of the MarR superfamily. PLoS ONE 14(2): e0210123. doi: 10.1371/journal.pone.0210123 | Institute for Integrative Biology of the Cell, CEA, CNRS, Université Paris-Saclay, Gif-sur-Yvette, France | https://doi.org/10.1371/journal.pone.0210123 | 1 | 2019 | Paloma Varela 2019. Holo-ZitRMG binding to dsDNA fragments by ITC. protocols.io dx.doi.org/10.17504/protocols.io.vg5e3y6 | 2021-03-29 03:08:47 | |||
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How to Prepare a Single Cell Suspension from a Frozen Cell Sample Resource Report Resource Website |
Stemcell Technologies | 10.17504/protocols.io.4umgwu6 | STEMCELL Technologies | Preparing a single cell suspension from a frozen starting sample is critical for optimizing cell isolations by avoiding additional cell loss and enabling the maximum labeling of target cells. Performing cell separation on clumpy cell samples can result in lower recovery and may interfere with proper labeling of the target cells. Samples may sometimes appear "clumpy" when they have been exposed to repeated freeze/thaw cycles or enzymatic tissue dissociation. These cell clumps occur because environmental stresses can accelerate the rate of cell death within the sample, resulting in the release of "sticky" DNA molecules from the dying cells that can clump neighboring cells together. Adding the endonuclease deoxyribonuclease I (DNase I) into your sample can minimize the presence of free-floating DNA fragments and cell clumps. This protocol describes a method to harvest cells and prepare a clump-free single cell suspension from a frozen cell sample prior to performing cell separation. | STEMCELL Technologies | 1 | 2019 | Stemcell Technologies 2019. How to Prepare a Single Cell Suspension from a Frozen Cell Sample. protocols.io dx.doi.org/10.17504/protocols.io.4umgwu6 | 2021-03-29 03:08:47 | |||
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MRI pilot Resource Report Resource Website |
Lukas Snoek, Tinka Beemsterboer | 10.17504/protocols.io.w8hfht6 | Spinoza Centre, REC-L | Run an MRI pilot at Spinoza REC.This protocol will help you running your pilot. Make sure to read this protocol before coming to the pilot so you know what is expected of you and which choices you will be going to make. | University of Amsterdam / Spinoza Centre for Neuroimaging, University of Amsterdam / Spinoza Centre for Neuroimaging | 2 | 2019 | Lukas Snoek, Tinka Beemsterboer 2019. MRI pilot. protocols.io dx.doi.org/10.17504/protocols.io.w8hfht6 | 2021-03-29 03:08:47 |
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