Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Dynamic distribution of gallbladder microbiota in rabbit at different ages and health states Short title: Dynamic distribution of gallbladder microbiota in rabbits Resource Report Resource Website |
Yawei Xing | 10.17504/protocols.io.xarfid6 | Xing Y, Liu J, Lu F, Wang L, Li Y, Ouyang C (2019) Dynamic distribution of gallbladder microbiota in rabbit at different ages and health states. PLoS ONE 14(2): e0211828. doi: 10.1371/journal.pone.0211828 | Chuihui Ouyang | https://doi.org/10.1371/journal.pone.0211828 | 1 | 2019 | Yawei Xing 2019. Dynamic distribution of gallbladder microbiota in rabbit at different ages and health states Short title: Dynamic distribution of gallbladder microbiota in rabbits. protocols.io dx.doi.org/10.17504/protocols.io.xarfid6 | 2021-03-29 03:08:44 | |||
|
Tache_Mulugeta_OT2OD024899_Colon tissue electrical stimulation and colonic motility measurements Resource Report Resource Website |
Muriel Larauche, Yushan Wang, Po-Min Wang, James Dunn, Wentai Liu, Mulugeta Million | 10.17504/protocols.io.3rmgm46 | SPARC | This protocol describes a process for the measurement of acute electrical stimulation-induced effects on colonic motility in anesthetized young adult Yucatan minipigs. Signals recorded from manometry probes inserted into the proximal, transverse and distal colonic regions were used to measure the effect of stimulation by serosally placed electrodes opposed to the colonic tissue at multiple sites in an acute anesthetized preparation. The effect of stimulation was quantified as motility index assessments before, during and after stimulation, and the data was used to create a functional map of colonic motor response to localized colon stimulation. | University of California, Los Angeles, University of California, Los Angeles, University of California, Los Angeles, Stanford University, University of California, Los Angeles, University of California, Los Angeles | 1 | 2020 | Muriel Larauche, Yushan Wang, Po-Min Wang, James Dunn, Wentai Liu, Mulugeta Million 2020. Tache_Mulugeta_OT2OD024899_Colon tissue electrical stimulation and colonic motility measurements. protocols.io dx.doi.org/10.17504/protocols.io.3rmgm46 | 2021-03-29 03:08:44 | |||
|
Rehabilitation protocol after varization osteotomy - Case Study Resource Report Resource Website |
Sebastião Santos | 10.17504/protocols.io.bi5kkg4w | Background: Knee rehabilitation using therapeutic exercises in the aquatic environment has been used in order to initiate lower limb rehabilitation earlier and improve joint movements, as it reduces pain and allows for earlier weight bearing. Objective: To develop and describe the effects of an aquatic therapeutic exercise program in a case study of tibial osteotomy and varus femur. Methods: A 15-year-old girl undergoing a tibial and femoral varus osteotomy completed an aquatic therapeutic exercise program consisting of a total of 30 sessions (3 sessions per week of 60 minutes). The subject was evaluated at two different times (at the beginning and end of the intervention). Results: There was an improvement in the various parameters evaluated (knee flexion: 66º vs 125º; knee extension: -7º vs -1º; pain on movement: 6/10 vs 0/10). Conclusion: The aquatic therapeutic exercise program used in this study has beneficial effects in a clinical case of tibial osteotomy and varus femur. | CIDESD (The Research Center in Sports Sciences, Health Sciences and Human Development), Vila Real, Portugal; Professor, Ministery of Education, Portugal | 1 | 2021 | Sebastião Santos 2021. Rehabilitation protocol after varization osteotomy - Case Study. protocols.io dx.doi.org/10.17504/protocols.io.bi5kkg4w | 2021-03-29 03:08:44 | ||||
|
Cold-leaching extraction. A new methodology for obtaining inhibitory substances produced by bacteria in solid media. Resource Report Resource Website |
Catherine Cesa-Luna, Alberto Aguayo-Acosta, Antonino Baez, Jesús Muñoz-Rojas, Verónica Quintero-Hernández | 10.17504/protocols.io.bhs3j6gn | Inhibitory substances can be obtained and purified by different methodologies. Free cell supernatants obtained by centrifugation and filtration are commonly used for evaluating antimicrobial metabolites produced by bacteria. In this work, a new methodology for obtaining antimicrobial metabolites is proposed, especially implemented for bacteria which are not able to produce these compounds in liquid media; based on the use of nitrocellulose filter membranes and solid culture media. | Ecology and Survival of Microorganisms Group (ESMG), Laboratorio de Ecología Molecular Microbiana (LEMM), Centro de Investigaciones en Ciencias Microbiológicas (CICM), Instituto de Ciencias (IC), Benemérita Universidad Autónoma de Puebla (BUAP), Puebla, Pue., México., Department of Microbiology and Immunology, Biological Sciences Faculty, Universidad Autónoma de Nuevo León, Ciudad Universitaria, San Nicolás de la Garza, Nuevo León, México., Ecology and Survival of Microorganisms Group (ESMG), Laboratorio de Ecología Molecular Microbiana (LEMM), Centro de Investigaciones en Ciencias Microbiológicas (CICM), Instituto de Ciencias (IC), Benemérita Universidad Autónoma de Puebla (BUAP), Puebla, Pue., México., Ecology and Survival of Microorganisms Group (ESMG), Laboratorio de Ecología Molecular Microbiana (LEMM), Centro de Investigaciones en Ciencias Microbiológicas (CICM), Instituto de Ciencias (IC), Benemérita Universidad Autónoma de Puebla (BUAP), Puebla, Pue., México., CONACYT – ESMG, LEMM, CICM, IC-BUAP, Puebla, Pue., México. | 4 | 2020 | Catherine Cesa-Luna, Alberto Aguayo-Acosta, Antonino Baez, Jesús Muñoz-Rojas, Verónica Quintero-Hernández 2020. Cold-leaching extraction. A new methodology for obtaining inhibitory substances produced by bacteria in solid media.. protocols.io dx.doi.org/10.17504/protocols.io.bhs3j6gn | 2021-03-29 03:08:45 | ||||
|
TRIS Acetate Phosphate Salts (x40) Resource Report Resource Website |
Steven Burgess | 10.17504/protocols.io.fcsbiwe | OpenPlant Project, Plantae | University of Cambridge | 1 | 2016 | Steven Burgess 2016. TRIS Acetate Phosphate Salts (x40). protocols.io dx.doi.org/10.17504/protocols.io.fcsbiwe | 2021-03-29 03:08:46 | ||||
|
Syntesis and kinetic study of phenol-formaldehyde resins synthesized in the presence of polyamines Resource Report Resource Website |
Magdalena Cygan, Mariusz Szemien, Stanisław Krompiec | 10.17504/protocols.io.ibecaje | Chemistry Method Development Community | Cygan M, Szemień M, Krompiec S (2018) Statistical screening analysis of the chemical composition and kinetic study of phenol-formaldehyde resins synthesized in the presence of polyamines as co-catalysts. PLoS ONE 13(5): e0195069. doi: 10.1371/journal.pone.0195069 | Pfleiderer Silekol Sp. z o.o and Department of Inorganic, Organometallic Chemistry and Catalysis, Institute of Chemistry, Faculty of Mathematics, Physics and Chemistry, University of Silesia, Pfleiderer Silekol Sp. z o.o and Department of Inorganic, Organometallic Chemistry and Catalysis, Institute of Chemistry, Faculty of Mathematics, Physics and Chemistry, University of Silesia, Pfleiderer Silekol Sp. z o.o and Department of Inorganic, Organometallic Chemistry and Catalysis, Institute of Chemistry, Faculty of Mathematics, Physics and Chemistry, University of Silesia | https://doi.org/10.1371/journal.pone.0195069 | 1 | 2017 | Magdalena Cygan, Mariusz Szemien, Stanisław Krompiec 2017. Syntesis and kinetic study of phenol-formaldehyde resins synthesized in the presence of polyamines . protocols.io dx.doi.org/10.17504/protocols.io.ibecaje | 2021-03-29 03:08:46 | ||
|
PHYTOHORMONE PROFILING BY LIQUID CHROMATOGRAPHY COUPLED TO MASS SPECTROMETRY (LC/MS) Resource Report Resource Website |
Camilo E. Vital, Jenny D. Gómez, Pedro M. Vidigal, Edvaldo Barros, Claudia S.L. Pontes, Nívea M. Vieira, Humberto J O Ramos | 10.17504/protocols.io.zgff3tn | Metabolomics Protocols & Workflows | Phytohormones play a key role in regulating development and growth, as well as acting on plant responses to biotic and abiotic stresses. The following protocol describes a target specific methodology to obtaining quantitative profiles of phytohormones from plant tissues by ultra-high-performance liquid chromatograph (UHPLC) coupled to mass spectrometry. | Center of Analysis of Biomolecules (NuBioMol), Universidade Federal de Viçosa - UFV, Viçosa-MG, Brazil, Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa - UFV, BIOAGRO/INCT-IPP, Viçosa-MG, Brazil, Center of Analysis of Biomolecules (NuBioMol), Universidade Federal de Viçosa - UFV, Viçosa-MG, Brazil, Center of Analysis of Biomolecules (NuBioMol), Universidade Federal de Viçosa - UFV, Viçosa-MG, Brazil, Center of Analysis of Biomolecules (NuBioMol), Universidade Federal de Viçosa - UFV, Viçosa-MG, Brazil, Center of Analysis of Biomolecules (NuBioMol), Universidade Federal de Viçosa - UFV, Viçosa-MG, Brazil, Center of Analysis of Biomolecules (NuBioMol), Universidade Federal de Viçosa - UFV, Viçosa-MG, Brazil | 2 | 2019 | Camilo E. Vital, Jenny D. Gómez, Pedro M. Vidigal, Edvaldo Barros, Claudia S.L. Pontes, Nívea M. Vieira, Humberto J O Ramos 2019. PHYTOHORMONE PROFILING BY LIQUID CHROMATOGRAPHY COUPLED TO MASS SPECTROMETRY (LC/MS). protocols.io dx.doi.org/10.17504/protocols.io.zgff3tn | 2021-03-29 03:08:48 | |||
|
10xV3 Genomics Sample Processing Protocol Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bq7cmziw | BICCN, Allen Institute for Brain Science | Protocol allows for rapid generation of 3’ transcriptomic-NGS-ready- single-cell-libraries from pools of cells.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2021 | Allen Institute for Brain Science 2021. 10xV3 Genomics Sample Processing Protocol. protocols.io dx.doi.org/10.17504/protocols.io.bq7cmziw | 2021-03-29 03:08:48 | |||
|
Laboratory protocols for the study “Whole genome analysis of selected human and animal rotaviruses identified in Uganda from 2012 to 2014 reveals complex genome re-assortment events between human, bovine, caprine and porcine strains”. Resource Report Resource Website |
Josephine Bwogi1, 2, Khuzwayo C. Jere3, 4 , Charles Karamagi2, Denis K. Byarugaba5, Prossy Namuwulya1, Frederick N. Baliraine6 , Ulrich Desselberger7 , Miren Iturriza-Gomara3. | 10.17504/protocols.io.h4vb8w6 | Bwogi J, Jere KC, Karamagi C, Byarugaba DK, Namuwulya P, Baliraine FN, Desselberger U, Iturriza-Gomara M (2017) Whole genome analysis of selected human and animal rotaviruses identified in Uganda from 2012 to 2014 reveals complex genome reassortment events between human, bovine, caprine and porcine strains. PLoS ONE 12(6): e0178855. doi: 10.1371/journal.pone.0178855 | 1 EPI laboratory, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda 2Department of Paediatrics and Child Health, College of Health Sciences, Makerere University, P.O. BOX 7072, Kampala Uganda 3 Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK 4 Malawi-Liverpool-Wellcome Trust Clinical Research Programme / Department of Medical Laboratory Sciences, University of Malawi, College of Medicine, Blantyre, Malawi 5 Department of Microbiology, College of Veterinary Medicine and Biosecurity, Makerere University, P.O.BOX 7072, Kampala, Uganda 6Department of Biology and Kinesiology, LeTourneau University, Longview, Texas, USA 7Department of Medicine, University of Cambridge, Cambridge, UK *Corresponding author: Josephine Bwogi, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda. Email: [email protected] or [email protected], 1 EPI laboratory, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda 2Department of Paediatrics and Child Health, College of Health Sciences, Makerere University, P.O. BOX 7072, Kampala Uganda 3 Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK 4 Malawi-Liverpool-Wellcome Trust Clinical Research Programme / Department of Medical Laboratory Sciences, University of Malawi, College of Medicine, Blantyre, Malawi 5 Department of Microbiology, College of Veterinary Medicine and Biosecurity, Makerere University, P.O.BOX 7072, Kampala, Uganda 6Department of Biology and Kinesiology, LeTourneau University, Longview, Texas, USA 7Department of Medicine, University of Cambridge, Cambridge, UK *Corresponding author: Josephine Bwogi, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda. Email: [email protected] or [email protected], 1 EPI laboratory, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda 2Department of Paediatrics and Child Health, College of Health Sciences, Makerere University, P.O. BOX 7072, Kampala Uganda 3 Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK 4 Malawi-Liverpool-Wellcome Trust Clinical Research Programme / Department of Medical Laboratory Sciences, University of Malawi, College of Medicine, Blantyre, Malawi 5 Department of Microbiology, College of Veterinary Medicine and Biosecurity, Makerere University, P.O.BOX 7072, Kampala, Uganda 6Department of Biology and Kinesiology, LeTourneau University, Longview, Texas, USA 7Department of Medicine, University of Cambridge, Cambridge, UK *Corresponding author: Josephine Bwogi, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda. Email: [email protected] or [email protected], 1 EPI laboratory, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda 2Department of Paediatrics and Child Health, College of Health Sciences, Makerere University, P.O. BOX 7072, Kampala Uganda 3 Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK 4 Malawi-Liverpool-Wellcome Trust Clinical Research Programme / Department of Medical Laboratory Sciences, University of Malawi, College of Medicine, Blantyre, Malawi 5 Department of Microbiology, College of Veterinary Medicine and Biosecurity, Makerere University, P.O.BOX 7072, Kampala, Uganda 6Department of Biology and Kinesiology, LeTourneau University, Longview, Texas, USA 7Department of Medicine, University of Cambridge, Cambridge, UK *Corresponding author: Josephine Bwogi, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda. Email: [email protected] or [email protected], 1 EPI laboratory, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda 2Department of Paediatrics and Child Health, College of Health Sciences, Makerere University, P.O. BOX 7072, Kampala Uganda 3 Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK 4 Malawi-Liverpool-Wellcome Trust Clinical Research Programme / Department of Medical Laboratory Sciences, University of Malawi, College of Medicine, Blantyre, Malawi 5 Department of Microbiology, College of Veterinary Medicine and Biosecurity, Makerere University, P.O.BOX 7072, Kampala, Uganda 6Department of Biology and Kinesiology, LeTourneau University, Longview, Texas, USA 7Department of Medicine, University of Cambridge, Cambridge, UK *Corresponding author: Josephine Bwogi, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda. Email: [email protected] or [email protected], 1 EPI laboratory, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda 2Department of Paediatrics and Child Health, College of Health Sciences, Makerere University, P.O. BOX 7072, Kampala Uganda 3 Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK 4 Malawi-Liverpool-Wellcome Trust Clinical Research Programme / Department of Medical Laboratory Sciences, University of Malawi, College of Medicine, Blantyre, Malawi 5 Department of Microbiology, College of Veterinary Medicine and Biosecurity, Makerere University, P.O.BOX 7072, Kampala, Uganda 6Department of Biology and Kinesiology, LeTourneau University, Longview, Texas, USA 7Department of Medicine, University of Cambridge, Cambridge, UK *Corresponding author: Josephine Bwogi, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda. Email: [email protected] or [email protected], 1 EPI laboratory, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda 2Department of Paediatrics and Child Health, College of Health Sciences, Makerere University, P.O. BOX 7072, Kampala Uganda 3 Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK 4 Malawi-Liverpool-Wellcome Trust Clinical Research Programme / Department of Medical Laboratory Sciences, University of Malawi, College of Medicine, Blantyre, Malawi 5 Department of Microbiology, College of Veterinary Medicine and Biosecurity, Makerere University, P.O.BOX 7072, Kampala, Uganda 6Department of Biology and Kinesiology, LeTourneau University, Longview, Texas, USA 7Department of Medicine, University of Cambridge, Cambridge, UK *Corresponding author: Josephine Bwogi, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda. Email: [email protected] or [email protected], 1 EPI laboratory, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda 2Department of Paediatrics and Child Health, College of Health Sciences, Makerere University, P.O. BOX 7072, Kampala Uganda 3 Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK 4 Malawi-Liverpool-Wellcome Trust Clinical Research Programme / Department of Medical Laboratory Sciences, University of Malawi, College of Medicine, Blantyre, Malawi 5 Department of Microbiology, College of Veterinary Medicine and Biosecurity, Makerere University, P.O.BOX 7072, Kampala, Uganda 6Department of Biology and Kinesiology, LeTourneau University, Longview, Texas, USA 7Department of Medicine, University of Cambridge, Cambridge, UK *Corresponding author: Josephine Bwogi, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda. Email: [email protected] or [email protected], 1 EPI laboratory, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda 2Department of Paediatrics and Child Health, College of Health Sciences, Makerere University, P.O. BOX 7072, Kampala Uganda 3 Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK 4 Malawi-Liverpool-Wellcome Trust Clinical Research Programme / Department of Medical Laboratory Sciences, University of Malawi, College of Medicine, Blantyre, Malawi 5 Department of Microbiology, College of Veterinary Medicine and Biosecurity, Makerere University, P.O.BOX 7072, Kampala, Uganda 6Department of Biology and Kinesiology, LeTourneau University, Longview, Texas, USA 7Department of Medicine, University of Cambridge, Cambridge, UK *Corresponding author: Josephine Bwogi, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda. Email: [email protected] or [email protected], 1 EPI laboratory, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda 2Department of Paediatrics and Child Health, College of Health Sciences, Makerere University, P.O. BOX 7072, Kampala Uganda 3 Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK 4 Malawi-Liverpool-Wellcome Trust Clinical Research Programme / Department of Medical Laboratory Sciences, University of Malawi, College of Medicine, Blantyre, Malawi 5 Department of Microbiology, College of Veterinary Medicine and Biosecurity, Makerere University, P.O.BOX 7072, Kampala, Uganda 6Department of Biology and Kinesiology, LeTourneau University, Longview, Texas, USA 7Department of Medicine, University of Cambridge, Cambridge, UK *Corresponding author: Josephine Bwogi, Uganda Virus Research Institute, 51-59 Nakiwogo Road, P.0.BOX 49, Entebbe, Uganda. Email: [email protected] or [email protected] | https://doi.org/10.1371/journal.pone.0178855 | 1 | 2017 | Josephine Bwogi1, 2, Khuzwayo C. Jere3, 4 , Charles Karamagi2, Denis K. Byarugaba5, Prossy Namuwulya1, Frederick N. Baliraine6 , Ulrich Desselberger7 , Miren Iturriza-Gomara3. 2017. Laboratory protocols for the study “Whole genome analysis of selected human and animal rotaviruses identified in Uganda from 2012 to 2014 reveals complex genome re-assortment events between human, bovine, caprine and porcine strains”.. protocols.io dx.doi.org/10.17504/protocols.io.h4vb8w6 | 2021-03-29 03:08:48 | |||
|
Separation and purification of human PBMC from FRESH BLOOD Resource Report Resource Website |
Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino | 10.17504/protocols.io.bfxmjpk6 | Separation and purification of PBMC from FRESH BLOOD: list of published work using this protocolKustrimovic, N., Comi, C., Magistrelli, L., Rasini, E., Legnaro, M., Bombelli, R., Aleksic, I., Blandini, F., Minafra, B., Riboldazzi, G., Sturchio, A., Mauri, M., Bono, G., Marino, F., & Cosentino, M. (2018). Parkinson's disease patients have a complex phenotypic and functional Th1 bias: cross-sectional studies of CD4+ Th1/Th2/T17 and Treg in drug-naïve and drug-treated patients. Journal of neuroinflammation, 15(1), 205. https://doi.org/10.1186/s12974-018-1248-8Kustrimovic, N., Rasini, E., Legnaro, M., Bombelli, R., Aleksic, I., Blandini, F., Comi, C., Mauri, M., Minafra, B., Riboldazzi, G., Sanchez-Guajardo, V., Marino, F., & Cosentino, M. (2016). Dopaminergic Receptors on CD4+ T Naive and Memory Lymphocytes Correlate with Motor Impairment in Patients with Parkinson's Disease. Scientific reports, 6, 33738. https://doi.org/10.1038/srep33738Cosentino M., Ferrari M., Kustrimovic N., Rasini E., Marino F. (2015). Influence of dopamine receptor gene polymorphisms on circulating T lymphocytes: A pilot study in healthy subjects. Human immunology, 76, 10, 747-752. https://doi.org/10.1016/j.humimm.2015.09.032 | Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy), Center for Research in Medical Pharmacology, University of Insubria, Center for Research in Medical Pharmacology, University of Insubria (Varese, Italy), University of Insubria, University of Insubria, University of Insubria, University of Insubria | 1 | 2020 | Marco Cosentino, Elisa Storelli, Alessandra Luini, Massimiliano Legnaro, Emanuela Rasini, Marco Ferrari, Franca Marino 2020. Separation and purification of human PBMC from FRESH BLOOD. protocols.io dx.doi.org/10.17504/protocols.io.bfxmjpk6 | 2021-03-29 03:08:49 | ||||
|
Use of tracer dyes to label neural projections to lower urinary tract organs [keast-001-stage01] Resource Report Resource Website |
Janet Keast, Peregrine Osborne | 10.17504/protocols.io.w3dfgi6 | SPARC | This protocol is used to visualise sensory and autonomic neurons innervating the bladder body (dome), bladder trigone or proximal urethra in an experimental adult male or female rat. The protocol is performed under anesthesia and should incorporate all local requirements for standards of animal experimentation, including methods of anesthesia, surgical environment, and post-operative monitoring and care. | University of Melbourne, University of Melbourne | 1 | 2019 | Janet Keast, Peregrine Osborne 2019. Use of tracer dyes to label neural projections to lower urinary tract organs [keast-001-stage01]. protocols.io dx.doi.org/10.17504/protocols.io.w3dfgi6 | 2021-03-29 03:08:46 | |||
|
Anti-c-Myc Tag (9E10) Affinity Gel Protocol Resource Report Resource Website |
Kelsey Knight | 10.17504/protocols.io.tideka6 | BioLegend | Catalog Number: 658502 Storage Temperature: 2°C-8°C Product Description Anti-c-Myc Tag (9E10) Affinity Gel consists of anti-c-myc monoclonal antibody (clone 9E10), covalently immobilized onto 6% high density glyoxal agarose beads. The affinity resin can be used in affinity purifying and immunoprecipitation of c-Myc-tagged fusion protein. Binding Specificity Mouse monoclonal antibody 9E10 recognizes the c-myc epitope N-EQKLSEEDL-C and is purified through Protein G chromatography. The antibody conjugated affinity resin can bind to epitope at N-terminal, C-terminal, and internal locations of a fusion protein. The binding capacity is greater than 0.5mg per ml of c-myc agarose resin. Reagent Anti-c-Myc Agarose Affinity Gel is supplied as a 50% suspension in phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide. Precautions and Disclaimer This product is for research use only. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. Storage/Stability Anti-c-Myc Agarose Affinity Gel should be stored between 2°C and 8°C for maximum stability. The unopened product is stable for one year when stored as indicated. After use, the resin should be regenerated and stored in TBS or PBS buffer (pH 7.2) containing 0.09% sodium azide. | BioLegend | https://www.biolegend.com/protocols/anti-c-myc-tag-9e10-affinity-gel-protocol/4297/ | 1 | 2018 | Kelsey Knight 2018. Anti-c-Myc Tag (9E10) Affinity Gel Protocol. protocols.io dx.doi.org/10.17504/protocols.io.tideka6 | 2021-03-29 03:08:46 | ||
|
Which preferences associate with school performance? – Lessons from a university exploratory study Resource Report Resource Website |
Daniel Horn, Hubert János Kiss | 10.17504/protocols.io.kepctdn | We attempt to link laboratory-based measures of preferences with measures of school performance. We measured in an incentivized way risk, time, social and competitive preferences and also cognitive abilities of university students and look for associations between these measures and two important academic outcome measures: exam results and GPA.We find consistently that cognitive abilities (proxied by the Cognitive Reflection Test (Frederick 2005)) are very well correlated with school performance. Regarding non-cognitive skills, we find suggestive evidence for many of our measured preferences. We used two alternative measures of time preference: patience and present bias. Present bias explains exam grades relatively better, while patience is better explaining GPA. Both measures of time preferences have a non-linear relation to school performance. Competitiveness matters, as students, who opt for a more competitive payment scheme in our experiments have a higher average GPA and better exam grades. We observe also that risk-averse students perform a little better than risk-loving students. That makes sense in case of multiple choice exams, because risk-loving students may want to try to pass the exam less prepared, as the possibility of passing as exam just by chance is not zero. Finally, we have also detected that cooperative preferences – the amount of money offered in a public good game – associates strongly with GPA, but in a non-linear way. Students who offered around half of their possible amounts had significantly higher GPAs than those, who offered none or all their money.Keywords: competititive preferences, experiment, non-cognitive skills, risk preferences, school performance, social preferences, time preferences.JEL codes: C91; D91; I20 | Horn D, Kiss HJ (2018) Which preferences associate with school performance?—Lessons from an exploratory study with university students. PLoS ONE 13(2): e0190163. doi: 10.1371/journal.pone.0190163 | MTA KRTK KTI & ELTE, MTA KRTK KTI & ELTE | https://doi.org/10.1371/journal.pone.0190163 | 1 | 2017 | Daniel Horn, Hubert János Kiss 2017. Which preferences associate with school performance? – Lessons from a university exploratory study. protocols.io dx.doi.org/10.17504/protocols.io.kepctdn | 2021-03-29 03:08:46 | ||
|
10 X Sucrose Orange G Loading Dye Resource Report Resource Website |
Gordon Wellman | 10.17504/protocols.io.kp7cvrn | Salt Lab KAUST | This protocol outlines the preparation of a basic sucrose loading dye 10 X stock solution for gel electrophoresis (DNA/RNA). Orange G runs at approximately 50 bp making this loading dye ideal for small molecular weight polynucleotides. Used mainly as an indicator for approx. 100 bp polynucleotides, where we would like to avoid contamination of small fragments with other loading dyes. It is also useful as an indicator if you have excessive primer-dimer amplification in your PCRs as these usually also run at approx. 50 bp.The origins of this protocol have been lost in the mists of time and this method been handed down from PhD student to PhD student. | King Abdullah University of Science and Technology (SA) | 1 | 2018 | Gordon Wellman 2018. 10 X Sucrose Orange G Loading Dye. protocols.io dx.doi.org/10.17504/protocols.io.kp7cvrn | 2021-03-29 03:08:48 | |||
|
microarray analysis Resource Report Resource Website |
Hajime Fujishima, Shoichi Fumoto, Tomotaka Shibata, Kohei Nishiki, Yoshiyuki Tsukamoto, Tsuyoshi Etoh, Masatsugu Moriyama, Norio Shiraishi, Masafumi Inomata | 10.17504/protocols.io.kahcsb6 | BackgroundRecently, neoadjuvant chemotherapy with docetaxel/cisplatin/5-fluorouracil (NAC-DCF) was identified as a novel strong regimen with a high rate of pathological complete response (pCR) in advanced esophageal cancer in Japan. Predicting pCR will contribute to the therapeutic strategy and the prevention of surgical invasion. However, a predictor of pCR after NAC-DCF has not yet been developed. The aim of this study was to identify a novel predictor of pCR in locally advanced esophageal cancer treated with NAC-DCF.Patients and MethodsA total of 32 patients who received NAC-DCF followed by esophagectomy between June 2013 and March 2016 were enrolled in this study. We divided the patients into the following 2 groups: pCR group (9 cases) and non-pCR group (23 cases), and compared gene expressions between these groups using DNA microarray data and KeyMolnet. Subsequently, a validation study of candidate molecular expression was performed in 7 additional cases.ResultsSeventeen molecules, including transcription factor E2F, T-cell-specific transcription factor, Src (known as “proto-oncogene tyrosine-protein kinase of sarcoma”), interferon regulatory factor 1, thymidylate synthase, cyclin B, cyclin-dependent kinase (CDK) 4, CDK, caspase-1, vitamin D receptor, histone deacetylase, MAPK/ERK kinase, bcl-2-associated X protein, runt-related transcription factor 1, PR domain zinc finger protein 1, platelet-derived growth factor receptor, and interleukin 1, were identified as candidate molecules. The molecules were mainly associated with pathways, such as transcriptional regulation by SMAD, RB/E2F, and STAT. The validation study indicated that 12 of the 17 molecules (71%) matched the trends of molecular expression.ConclusionsA 17-molecule set that predicts pCR after NAC-DCF for locally advanced esophageal cancer was identified. | Fujishima H, Fumoto S, Shibata T, Nishiki K, Tsukamoto Y, Etoh T, Moriyama M, Shiraishi N, Inomata M (2017) A 17-molecule set as a predictor of complete response to neoadjuvant chemotherapy with docetaxel, cisplatin, and 5-fluorouracil in esophageal cancer. PLoS ONE 12(11): e0188098. doi: 10.1371/journal.pone.0188098 | Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, Department of Surgery, Oita Nakamura Hospital, Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, Department of Surgery, Oita Nakamura Hospital, Department of Molecular Pathology, Oita University Faculty of Medicine, Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, Department of Molecular Pathology, Oita University Faculty of Medicine, Comprehensive Surgery for Community Medicine, Oita University Faculty of Medicine, Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine | https://doi.org/10.1371/journal.pone.0188098 | 1 | 2017 | Hajime Fujishima, Shoichi Fumoto, Tomotaka Shibata, Kohei Nishiki, Yoshiyuki Tsukamoto, Tsuyoshi Etoh, Masatsugu Moriyama, Norio Shiraishi, Masafumi Inomata 2017. microarray analysis. protocols.io dx.doi.org/10.17504/protocols.io.kahcsb6 | 2021-03-29 03:08:46 | ||
|
The 'Three Peaks' faecal DNA extraction method for long-read sequencing Resource Report Resource Website |
Josh Quick | 10.17504/protocols.io.584g9yw | High molecular weight DNA extraction from all kingdoms, Long Read Club | Sam Nicholls [University of Birmingham], Nicholas Loman [University of Birmingham] | https://www.slideshare.net/scalene/the-three-peak-challenge-for-longread-ultradeep-stool-metagenomics-on-the-promethion | 1 | 2019 | Josh Quick 2019. The 'Three Peaks' faecal DNA extraction method for long-read sequencing. protocols.io dx.doi.org/10.17504/protocols.io.584g9yw | 2021-03-29 03:08:46 | |||
|
Extraction and detection of free-gossypol in cottonseed samples Resource Report Resource Website |
Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio de Aquino Ribeiro, Felix Gonçalves de Siqueira, Robert Neil Gerard Miller, Simone Mendonça | 10.17504/protocols.io.krncv5e | This simple and rapid chromatographic detection method was developed and validated in order to accurately quantify trace levels of free gossypol in different cotton materials, including cottonseed, cottonseed cake and cottonseed cake treated with macrofungi. | Conceição AA, Neto CBS, Ribeiro JAdA, Siqueira FGd, Miller RNG, Mendonça S (2018) Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake. PLoS ONE 13(5): e0196164. doi: 10.1371/journal.pone.0196164 | Universidade Federal da Bahia, Universidade de Brasilia, Embrapa Agroenergia, Embrapa Agroenergia, Universidade de Brasilia, Embrapa Agroenergia | https://doi.org/10.1371/journal.pone.0196164 | 1 | 2018 | Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio de Aquino Ribeiro, Felix Gonçalves de Siqueira, Robert Neil Gerard Miller, Simone Mendonça 2018. Extraction and detection of free-gossypol in cottonseed samples. protocols.io dx.doi.org/10.17504/protocols.io.krncv5e | 2021-03-29 03:08:46 | ||
|
Natural Transformation Resource Report Resource Website |
Franziska Müller, Memduha Muratoglu, Jana Jung | 10.17504/protocols.io.uyzexx6 | Following this protocol, V. Natriegens cells can be transformed with linear fragments and plasmids based on natural transformation as described in Multiplex Genome Editing by Natural Transformation (MuGENT) for Synthetic Biology in Vibrio natriegens Dalia et. al, 2017. ACS Synthetic Biology. | Philipps-Universität Marburg, Philipps-Universität Marburg, Philipps-Universität Marburg | 1 | 2018 | Franziska Müller, Memduha Muratoglu, Jana Jung 2018. Natural Transformation. protocols.io dx.doi.org/10.17504/protocols.io.uyzexx6 | 2021-03-29 03:08:46 | ||||
|
Running the PTC-0200 DNA Engine Resource Report Resource Website |
Dr. Steven Wilhelm, Alyssa Alsante | 10.17504/protocols.io.in7cdhn | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol. | , | 1 | 2017 | Dr. Steven Wilhelm, Alyssa Alsante 2017. Running the PTC-0200 DNA Engine. protocols.io dx.doi.org/10.17504/protocols.io.in7cdhn | 2021-03-29 03:08:46 | |||
|
Dundee peripheral blood mononuclear cell (PBMC) isolation protocol (from whole blood) Resource Report Resource Website |
Adil R. Sarhan, Andrew J.M. Howden, Dario R. Alessi, Esther M. Sammler | 10.17504/protocols.io.bnhxmb7n | We describe a fast and efficient peripheral blood mononuclear cell (PBMC) isolation for LRRK2 kinase pathway analysis .justify:after { content: ""; display:inline-block; width: 100%; } Gain of kinase function mutations in the Leucine rich repeat kinase 2 (LRRK2) are associated with causing Parkinson’s disease. LRRK2 phosphorylates a subgroup of Rab GTPases and their phosphorylation levels mirror LRRK2 kinase activation status. Here, we describe a facile and robust method for isolating PBMCs by facilitated density gradient centrifugation, subsequent treatment with and without the specific LRRK2 kinase inhibitor MLi-2 to ensure that any effect seen is LRRK2 kinase dependent and cell lysis. PBMC lysates can then be used to quantify LRRK2 kinase pathway activity by measuring LRRK2-mediated phosphorylation of its endogenous RabGTPase substrates in human peripheral blood neutrophils either by quantitative immunoblotting or targeted mass-spectrometry. The benefits of using PBMCs are that they are routinely used as a biomatrix for Parkinson’s research and their isolation procedure is significantly faster and easier than that for example of neutrophils or monocytes from peripheral blood. However, their heterogeneity in terms of cellular composition and highly variable expression levels of LRRK2 and its substrates including Rab10 make PBMC a suboptimal biomatrix for reliably measuring LRRK2 kinase pathway activity. A potential use could be target engagement studies in clinical trials targeting LRRK2. .justify:after { content: ""; display:inline-block; width: 100%; } | Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK, Division of Cell Signaling and Immunology, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK, Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK, Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK | 1 | 2021 | Adil R. Sarhan, Andrew J.M. Howden, Dario R. Alessi, Esther M. Sammler 2021. Dundee peripheral blood mononuclear cell (PBMC) isolation protocol (from whole blood). protocols.io dx.doi.org/10.17504/protocols.io.bnhxmb7n | 2021-03-29 03:08:47 |
Can't find your Protocol?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific protocol and you know the DOI of the protocol already, it's easier to enter a DOI to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your protocol in the search results, please help us by adding it into the system — it's easy. Create and publish your protocols at Protocols.io.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
