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Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from Wetzel and Likens 2000. Limnological Analyses, Springer NY
Proper citation: Dr. Steven Wilhelm 2017. Total Chlorophyll a Measurements by Spectrophotometer. protocols.io dx.doi.org/10.17504/protocols.io.ijpccmn Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from Ichimura, T. 1979 2. Isolation and culture methods of algae. 2.5.B. Freshwater algae [2. Sôrui no bunri to baiyôhô. 2.5.B. Tansui sôrui]. In Methods in Phycological Studies [Sôrui Kenkyûhô], Eds. by Nishizawa, K. & Chihara, M., Kyoritsu Shuppan, Tokyo, p. 294-305 ( in Japanese without English title ).
Proper citation: Dr. Steven Wilhelm 2017. MA Media. protocols.io dx.doi.org/10.17504/protocols.io.h9jb94n Copy
Authors: Ashley Humphrey
Group: Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Proper citation: Ashley Humphrey 2016. ESAW Media for Marine Phytoplankton. protocols.io dx.doi.org/10.17504/protocols.io.gc9bsz6 Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from Schwyn, B. & Neilands, J. B. Universal chemical assay for the detection and determination of siderophores.Anal Biochem, 160:147-156 (1987).
Proper citation: Dr. Steven Wilhelm 2017. Chrome Azural S (CAS) Plate Assay for Iron-Binding Compounds. protocols.io dx.doi.org/10.17504/protocols.io.idvca66 Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.
Proper citation: Dr. Steven Wilhelm 2019. Phage Buffer. protocols.io dx.doi.org/10.17504/protocols.io.iitccen Copy
Authors: Dr. Steven Wilhelm, Josh Stough
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs
Summary: Contact Dr. Steven Wilhelm ([email protected]) or Josh Stough ([email protected]) for additional information regarding this protocol.
Proper citation: Dr. Steven Wilhelm, Josh Stough 2017. Screening Sequencing Datasets for Marker Genes in CLC Genomics. protocols.io dx.doi.org/10.17504/protocols.io.hgsb3we Copy
Authors: Ashley Humphrey
Group: Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Defined Medium for Aureococcus anophagefferens
Proper citation: Ashley Humphrey 2017. ASP12A Recipe for culturing Aureococcus anophagefferens. protocols.io dx.doi.org/10.17504/protocols.io.g29byh6 Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from the Handbook of Methods in Aquatic Microbial Ecology (p. 220)
Proper citation: Dr. Steven Wilhelm 2017. Acridine Orange Staining. protocols.io dx.doi.org/10.17504/protocols.io.ib3caqn Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for more information regarding this protocol.Modified from Pardee, A. B., F. Jacob, and J. Monod. 1959. The genetic control and cytoplasmic expression of "inducibility" in the synthesis of ß-galactosidase in E. coli. J. Mol. Biol. 1:165-178
Proper citation: Dr. Steven Wilhelm 2017. M9 Salts. protocols.io dx.doi.org/10.17504/protocols.io.ibwcape Copy
Authors: Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.
Proper citation: Steven Wilhelm 2017. Fluorescent Staining of T5-phages. protocols.io dx.doi.org/10.17504/protocols.io.iksccwe Copy
Authors: Steven Wilhelm, Robbie Martin
Group: Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs, Great Lakes Center for Fresh Waters and Human Health
Summary: Contact Dr. Steven Wilhelm ([email protected]) or Robbie Martin ([email protected]) for additional information regarding this protocol.
Proper citation: Steven Wilhelm, Robbie Martin 2017. Hot-phenol RNA Extraction. protocols.io dx.doi.org/10.17504/protocols.io.g89bzz6 Copy
Authors: Samantha Coy, Alyssa Alsante, Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols
Summary: Contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol.
Proper citation: Samantha Coy, Alyssa Alsante, Steven Wilhelm 2018. Long term Cryopreservation of Chloroviruses by Infection of Chlorella. protocols.io dx.doi.org/10.17504/protocols.io.wa2fage Copy
Authors: Steven Wilhelm, Samantha Coy, Alyssa Alsante
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols
Summary: Adapted from: Ortmann and Suttle (2009) Determination of Virus Abundance by Epifluorescence microscopy. Ch. 10 Methods of Molecular Biology. Contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol.
Proper citation: Steven Wilhelm, Samantha Coy, Alyssa Alsante 2018. Epifluorescent Microscopy of Virus Particles Using SYBR Green. protocols.io dx.doi.org/10.17504/protocols.io.qsgdwbw Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from Schwyn, B. & Neilands, J. B. Universal chemical assay for the detection and determination of siderophores.Anal Biochem, 160:147-156 (1987).
Proper citation: Dr. Steven Wilhelm 2017. Chrome Azurol S (CAS) Assays for Iron-Binding Compounds. protocols.io dx.doi.org/10.17504/protocols.io.ib6care Copy
Authors: Brittany Zepernick, Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Great Lakes Center for Fresh Waters and Human Health
Summary: The FlowCAM is a useful tool for morphological and physiological parameter analysis as well as identification of plankton. With over 30 different measurements available per cell, it facilitates cutting-edge analyses of environmental and cultural populations. To date, it has been generally employed for phytoplankton studies.
Proper citation: Brittany Zepernick, Steven Wilhelm 2019. Flow CAM 8000 Operation Protocol/Sample. protocols.io dx.doi.org/10.17504/protocols.io.74qhqvw Copy
Authors: Samantha Coy, Alyssa Alsante, Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol.
Proper citation: Samantha Coy, Alyssa Alsante, Steven Wilhelm 2018. Long term Cryopreservation of Chloroviruses by Infection of Chlorella. protocols.io dx.doi.org/10.17504/protocols.io.tfuejnw Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.
Proper citation: Dr. Steven Wilhelm 2017. Bacterial Cryopreservation. protocols.io dx.doi.org/10.17504/protocols.io.ihncb5e Copy
Authors: Lauren Krausfeldt
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: RNA extraction from water collected on Sterivex. The DNeasy PowerWater Sterivex kit is used and modified specifically for RNA. Also includes modifications for samples preserved in RNAlater.
Proper citation: Lauren Krausfeldt 2017. RNA Extraction from Sterivex filters . protocols.io dx.doi.org/10.17504/protocols.io.gmkbu4w Copy
Authors: Dr. Steven Wilhelm, Samantha Coy
Group: Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols
Summary: Van Etten, J. (n.d.). Formulation of Modified Bold's Basal Medium (MBBM). Retrieved from http://ncv.unl.edu/vanettenlab/
Contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol.
Proper citation: Dr. Steven Wilhelm, Samantha Coy 2017. MBBM Stock Solutions. protocols.io dx.doi.org/10.17504/protocols.io.hgtb3wn Copy
Authors: Ashley Humphrey
Group: Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Trace metals solution to be used in ESAW Media for Marine Phytoplankton
Proper citation: Ashley Humphrey 2016. Trace Metal Solution for ESAW Media. protocols.io dx.doi.org/10.17504/protocols.io.ge8bthw Copy
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