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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
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Total Chlorophyll a Measurements by Spectrophotometer Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.ijpccmn | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from Wetzel and Likens 2000. Limnological Analyses, Springer NY | 1 | 2017 | Dr. Steven Wilhelm 2017. Total Chlorophyll a Measurements by Spectrophotometer. protocols.io dx.doi.org/10.17504/protocols.io.ijpccmn | 2021-03-29 03:08:07 | ||||
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MA Media Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.h9jb94n | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from Ichimura, T. 1979 2. Isolation and culture methods of algae. 2.5.B. Freshwater algae [2. Sôrui no bunri to baiyôhô. 2.5.B. Tansui sôrui]. In Methods in Phycological Studies [Sôrui Kenkyûhô], Eds. by Nishizawa, K. & Chihara, M., Kyoritsu Shuppan, Tokyo, p. 294-305 ( in Japanese without English title ). | 1 | 2017 | Dr. Steven Wilhelm 2017. MA Media. protocols.io dx.doi.org/10.17504/protocols.io.h9jb94n | 2021-03-29 03:08:03 | ||||
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ESAW Media for Marine Phytoplankton Resource Report Resource Website |
Ashley Humphrey | 10.17504/protocols.io.gc9bsz6 | Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | University of Tennessee, Microbiology. | 3 | 2016 | Ashley Humphrey 2016. ESAW Media for Marine Phytoplankton. protocols.io dx.doi.org/10.17504/protocols.io.gc9bsz6 | 2021-03-29 03:11:36 | ||||
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Chrome Azural S (CAS) Plate Assay for Iron-Binding Compounds Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.idvca66 | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from Schwyn, B. & Neilands, J. B. Universal chemical assay for the detection and determination of siderophores.Anal Biochem, 160:147-156 (1987). | 1 | 2017 | Dr. Steven Wilhelm 2017. Chrome Azural S (CAS) Plate Assay for Iron-Binding Compounds. protocols.io dx.doi.org/10.17504/protocols.io.idvca66 | 2021-03-29 03:11:35 | ||||
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Phage Buffer Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.iitccen | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol. | Microbiology Department. University of Tennessee, Knoxville. | 1 | 2019 | Dr. Steven Wilhelm 2019. Phage Buffer. protocols.io dx.doi.org/10.17504/protocols.io.iitccen | 2021-03-29 03:11:43 | |||
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Screening Sequencing Datasets for Marker Genes in CLC Genomics Resource Report Resource Website |
Dr. Steven Wilhelm, Josh Stough | 10.17504/protocols.io.hgsb3we | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs | Contact Dr. Steven Wilhelm ([email protected]) or Josh Stough ([email protected]) for additional information regarding this protocol. | , | 2 | 2017 | Dr. Steven Wilhelm, Josh Stough 2017. Screening Sequencing Datasets for Marker Genes in CLC Genomics. protocols.io dx.doi.org/10.17504/protocols.io.hgsb3we | 2021-03-29 03:11:43 | |||
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ASP12A Recipe for culturing Aureococcus anophagefferens Resource Report Resource Website |
Ashley Humphrey | 10.17504/protocols.io.g29byh6 | Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Defined Medium for Aureococcus anophagefferens | University of Tennessee, Knoxville | http://www.nefsc.noaa.gov/nefsc/publications/crd/crd0517/crd0517.pdf | 1 | 2017 | Ashley Humphrey 2017. ASP12A Recipe for culturing Aureococcus anophagefferens. protocols.io dx.doi.org/10.17504/protocols.io.g29byh6 | 2021-03-29 03:11:42 | ||
|
Acridine Orange Staining Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.ib3caqn | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from the Handbook of Methods in Aquatic Microbial Ecology (p. 220) | 1 | 2017 | Dr. Steven Wilhelm 2017. Acridine Orange Staining. protocols.io dx.doi.org/10.17504/protocols.io.ib3caqn | 2021-03-29 03:08:52 | ||||
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M9 Salts Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.ibwcape | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for more information regarding this protocol.Modified from Pardee, A. B., F. Jacob, and J. Monod. 1959. The genetic control and cytoplasmic expression of "inducibility" in the synthesis of ß-galactosidase in E. coli. J. Mol. Biol. 1:165-178 | 1 | 2017 | Dr. Steven Wilhelm 2017. M9 Salts. protocols.io dx.doi.org/10.17504/protocols.io.ibwcape | 2021-03-29 03:08:52 | ||||
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Fluorescent Staining of T5-phages Resource Report Resource Website |
Steven Wilhelm | 10.17504/protocols.io.iksccwe | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol. | The University of Tennessee, Knoxville | 1 | 2017 | Steven Wilhelm 2017. Fluorescent Staining of T5-phages. protocols.io dx.doi.org/10.17504/protocols.io.iksccwe | 2021-03-29 03:09:19 | |||
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Hot-phenol RNA Extraction Resource Report Resource Website |
Steven Wilhelm, Robbie Martin | 10.17504/protocols.io.g89bzz6 | Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs, Great Lakes Center for Fresh Waters and Human Health | Contact Dr. Steven Wilhelm ([email protected]) or Robbie Martin ([email protected]) for additional information regarding this protocol. | Adapted from a protocol provided by Dr. Elizabeth Fozo, Adapted from a protocol provided by Dr. Elizabeth Fozo | 1 | 2017 | Steven Wilhelm, Robbie Martin 2017. Hot-phenol RNA Extraction. protocols.io dx.doi.org/10.17504/protocols.io.g89bzz6 | 2021-03-29 03:09:24 | |||
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Long term Cryopreservation of Chloroviruses by Infection of Chlorella Resource Report Resource Website |
Samantha Coy, Alyssa Alsante, Steven Wilhelm | 10.17504/protocols.io.wa2fage | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols | Contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol. | The University of Tennessee, Knoxville, The University of Tennessee, Knoxville, The University of Tennessee, Knoxville | 4 | 2018 | Samantha Coy, Alyssa Alsante, Steven Wilhelm 2018. Long term Cryopreservation of Chloroviruses by Infection of Chlorella. protocols.io dx.doi.org/10.17504/protocols.io.wa2fage | 2021-03-29 03:09:25 | |||
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Epifluorescent Microscopy of Virus Particles Using SYBR Green Resource Report Resource Website |
Steven Wilhelm, Samantha Coy, Alyssa Alsante | 10.17504/protocols.io.qsgdwbw | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols | Adapted from: Ortmann and Suttle (2009) Determination of Virus Abundance by Epifluorescence microscopy. Ch. 10 Methods of Molecular Biology. Contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol. | The University of Tennessee, Knoxville, The University of Tennessee, Knoxville, The University of Tennessee, Knoxville | https://doi.org/10.1371/journal.pone.0184371 | 5 | 2018 | Steven Wilhelm, Samantha Coy, Alyssa Alsante 2018. Epifluorescent Microscopy of Virus Particles Using SYBR Green. protocols.io dx.doi.org/10.17504/protocols.io.qsgdwbw | 2021-03-29 03:07:41 | ||
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Chrome Azurol S (CAS) Assays for Iron-Binding Compounds Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.ib6care | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from Schwyn, B. & Neilands, J. B. Universal chemical assay for the detection and determination of siderophores.Anal Biochem, 160:147-156 (1987). | 1 | 2017 | Dr. Steven Wilhelm 2017. Chrome Azurol S (CAS) Assays for Iron-Binding Compounds. protocols.io dx.doi.org/10.17504/protocols.io.ib6care | 2021-03-29 03:10:35 | ||||
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Flow CAM 8000 Operation Protocol/Sample Resource Report Resource Website |
Brittany Zepernick, Steven Wilhelm | 10.17504/protocols.io.74qhqvw | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Great Lakes Center for Fresh Waters and Human Health | The FlowCAM is a useful tool for morphological and physiological parameter analysis as well as identification of plankton. With over 30 different measurements available per cell, it facilitates cutting-edge analyses of environmental and cultural populations. To date, it has been generally employed for phytoplankton studies. | University of Tennessee, Knoxville, The University of Tennessee, Knoxville | http://wilhelmlab.utk.edu/ | 1 | 2019 | Brittany Zepernick, Steven Wilhelm 2019. Flow CAM 8000 Operation Protocol/Sample. protocols.io dx.doi.org/10.17504/protocols.io.74qhqvw | 2021-03-29 03:11:12 | ||
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Long term Cryopreservation of Chloroviruses by Infection of Chlorella Resource Report Resource Website |
Samantha Coy, Alyssa Alsante, Steven Wilhelm | 10.17504/protocols.io.tfuejnw | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol. | The University of Tennessee, Knoxville, The University of Tennessee, Knoxville, The University of Tennessee, Knoxville | 3 | 2018 | Samantha Coy, Alyssa Alsante, Steven Wilhelm 2018. Long term Cryopreservation of Chloroviruses by Infection of Chlorella. protocols.io dx.doi.org/10.17504/protocols.io.tfuejnw | 2021-03-29 03:12:32 | |||
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Bacterial Cryopreservation Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.ihncb5e | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol. | 1 | 2017 | Dr. Steven Wilhelm 2017. Bacterial Cryopreservation. protocols.io dx.doi.org/10.17504/protocols.io.ihncb5e | 2021-03-29 03:12:45 | ||||
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RNA Extraction from Sterivex filters Resource Report Resource Website |
Lauren Krausfeldt | 10.17504/protocols.io.gmkbu4w | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | RNA extraction from water collected on Sterivex. The DNeasy PowerWater Sterivex kit is used and modified specifically for RNA. Also includes modifications for samples preserved in RNAlater. | https://mobio.com/media/wysiwyg/pdfs/protocols/14600-S.pdf | 1 | 2017 | Lauren Krausfeldt 2017. RNA Extraction from Sterivex filters . protocols.io dx.doi.org/10.17504/protocols.io.gmkbu4w | 2021-03-29 03:12:45 | |||
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MBBM Stock Solutions Resource Report Resource Website |
Dr. Steven Wilhelm, Samantha Coy | 10.17504/protocols.io.hgtb3wn | Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols | Van Etten, J. (n.d.). Formulation of Modified Bold's Basal Medium (MBBM). Retrieved from http://ncv.unl.edu/vanettenlab/ Contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol. | , | http://ncv.unl.edu/vanettenlab/ | 2 | 2017 | Dr. Steven Wilhelm, Samantha Coy 2017. MBBM Stock Solutions. protocols.io dx.doi.org/10.17504/protocols.io.hgtb3wn | 2021-03-29 03:12:51 | ||
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Trace Metal Solution for ESAW Media Resource Report Resource Website |
Ashley Humphrey | 10.17504/protocols.io.ge8bthw | Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Trace metals solution to be used in ESAW Media for Marine Phytoplankton | University of Tennessee, Knoxville | 2 | 2016 | Ashley Humphrey 2016. Trace Metal Solution for ESAW Media. protocols.io dx.doi.org/10.17504/protocols.io.ge8bthw | 2021-03-29 03:12:56 |
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