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Authors: Isabel Gautreau
Group: New England Biolabs (NEB)
Proper citation: Isabel Gautreau 2020. NEBNext End Repair Module E6050. protocols.io dx.doi.org/10.17504/protocols.io.k64czgw Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the Double Digest Protocol with Standard Restriction Enzymes, using a common reaction and same incubation temperature for both enzymes.
Proper citation: New England Biolabs 2014. Single-temperature Double Digest. protocols.io dx.doi.org/10.17504/protocols.io.cjhuj5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010).Quickly and easily purify up to 100 µg of high-quality total RNA from multiple sample types – all with one kit!For use with blood, cells and tissuesAlso works with tough to lyse samples (bacteria, yeast, plant)Effectively purifies total RNA of all sizes, including small RNAs >20 ntEfficient genomic DNA removal (column and DNase I-based)Contains Proteinase K for processing of tissues and blood samplesIncludes RNA Protection Reagent for sample preservationExcellent valueKit components available separately
Proper citation: New England Biolabs 2018. Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010). protocols.io dx.doi.org/10.17504/protocols.io.nyadfse Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the quick version of the Monarch® DNA Gel Extraction Kit Protocol (NEB #T1020). For the full protocol, please click here.
Proper citation: New England Biolabs 2020. Quick Protocol for Monarch® DNA Gel Extraction Kit (NEB #T1020). protocols.io dx.doi.org/10.17504/protocols.io.bp9gmr3w Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA. This protocol describes how to determine genome targeting efficiency by digesting annealed PCR products with T7 Endonuclease I. In the first step PCR products are produced from the genomic DNA of cells whose genomes were targeted using Cas9, TALEN, ZFN etc. In the second step, the PCR products are annealed and digested with T7 Endonuclease I. Fragments are analyzed to determine the efficiency of genome targeting.
Proper citation: New England Biolabs 2016. Determining Genome Targeting Efficiency using T7 Endonuclease I (M0302). protocols.io dx.doi.org/10.17504/protocols.io.etbbein Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Mixture for PCR Amplification (E7370). protocols.io dx.doi.org/10.17504/protocols.io.cg7tzm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the synthesis protocol using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (E2050)
Proper citation: New England Biolabs 2015. Standard RNA Synthesis (E2050). protocols.io dx.doi.org/10.17504/protocols.io.cr2v8d Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocol is for First Strand Synthesis with Reverse Transcriptase
Proper citation: New England Biolabs 2015. First Strand Synthesis with Reverse Transcriptase. protocols.io dx.doi.org/10.17504/protocols.io.cjuunv Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the correct protocol if you are using the C3019I cells. If you are using the C3019H cells, please refer to this protocol.
Proper citation: New England Biolabs 2017. High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019I). protocols.io dx.doi.org/10.17504/protocols.io.jxzcpp6 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Loading Marker Mixture. protocols.io dx.doi.org/10.17504/protocols.io.cq5vy5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010).Quickly and easily purify up to 100 µg of high-quality total RNA from multiple sample types – all with one kit!For use with blood, cells and tissuesAlso works with tough to lyse samples (bacteria, yeast, plant)Effectively purifies total RNA of all sizes, including small RNAs >20 ntEfficient genomic DNA removal (column and DNase I-based)Contains Proteinase K for processing of tissues and blood samplesIncludes RNA Protection Reagent for sample preservationExcellent valueKit components available separately
Proper citation: New England Biolabs 2020. Quick Protocol for Monarch® Total RNA Miniprep Kit (NEB #T2010). protocols.io dx.doi.org/10.17504/protocols.io.bp9cmr2w Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2014. Mixture for M0290 CIP. protocols.io dx.doi.org/10.17504/protocols.io.chrt55 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocols is to be performed with the Quick Ligation Reaction Buffer. Please see the NEB website for more information.
Proper citation: New England Biolabs 2017. Quick Ligation Protocol (M2200). protocols.io dx.doi.org/10.17504/protocols.io.iqvcdw6 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. PNGase F Mixture 2. protocols.io dx.doi.org/10.17504/protocols.io.cqhvt5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of RNA into high quality directional (strand-specific) libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow has minimal hands-on time and is compatible with poly(A) mRNA enrichment and rRNA depletion methods.
Proper citation: New England Biolabs 2018. Protocol for use with FFPE RNA, NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765). protocols.io dx.doi.org/10.17504/protocols.io.tumenu6 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA. This protocol describes how to determine genome targeting efficiency by digesting annealed PCR products with T7 Endonuclease I. In the first step PCR products are produced from the genomic DNA of cells whose genomes were targeted using Cas9, TALEN, ZFN etc. In the second step, the PCR products are annealed and digested with T7 Endonuclease I. Fragments are analyzed to determine the efficiency of genome targeting.
Proper citation: New England Biolabs 2015. Determining Genome Targeting Efficiency using T7 Endonuclease I (M0302). protocols.io dx.doi.org/10.17504/protocols.io.cqpvvm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II FS DNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of DNA into high quality libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow also has minimal hands-on time.Note: The Ultra II FS Kit is not compatible with bisulfite conversion workflows.
Proper citation: New England Biolabs 2021. E7805 NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® Protocol for Large Fragment Sizes (> 550 bp) (Chapter 3). protocols.io dx.doi.org/10.17504/protocols.io.n2idgce Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for the removal of single-stranded extension using Mung Bean Nuclease (M0250).
Proper citation: New England Biolabs 2015. Removal of Single-Stranded Extension using Mung Bean Nuclease (M0250). protocols.io dx.doi.org/10.17504/protocols.io.crhv35 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: PCR Protocol for Taq DNA Polymerase with ThermoPol® Buffer (M0267)
Proper citation: New England Biolabs 2015. PCR with Taq and ThermoPol® Buffer (M0267). protocols.io dx.doi.org/10.17504/protocols.io.cifubm Copy
Authors: New England Biolabs, Menna Teffera
Group: New England Biolabs (NEB)
Summary: The NEBNext dA-Tailing Module has been optimized to efficiently incorporate a non-templated dAMP on the 3 ́ end of a blunt DNA fragment (1). 3 ́-dA DNA tailing prevents concadamer formation during subsequent ligation steps.
DNA tailed with the NEBNext dA-Tailing module may be ligated to adaptors or cloning vectors with complementary dT overhangs. The NEBNext dA-Tailing Module is provided as a master mix to maximize efficiency and convenience in DNA sample preparation workflows.
The NEBNext dA-Tailing Module has been validated by sequencing with the Il- lumina Genome Analyzer II (Illumina, Inc.) in conjunction with the NEBNext End Repair Module, NEBNext Quick Ligation Module and Phusion® High-Fidelity PCR Master Mix.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
Proper citation: New England Biolabs, Menna Teffera 2019. NEBNext dA-Tailing Module (NEB #E6053). protocols.io dx.doi.org/10.17504/protocols.io.4t3gwqn Copy
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