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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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U Cinn - Cholesterol Concentration Resource Report Resource Website |
Patrick Tso, Dana Lee | 10.17504/protocols.io.xiifkce | Mouse Metabolic Phenotyping Centers | In vitro quantification of cholesterol in serum or plasma is determined using an Infinity Total Cholesterol Assay kit. This assay enzymatically hydrolyzes the cholesterol esters in the sample to cholesterol and free fatty acids. The free cholesterol is further oxidized and combined with HBA to allow it to be quantitatively measured in the serum or plasma. | University of Cincinnati, University of Cincinnati | https://mmpc.org/shared/document.aspx?id=199&docType=Protocol | 1 | 2019 | Patrick Tso, Dana Lee 2019. U Cinn - Cholesterol Concentration. protocols.io dx.doi.org/10.17504/protocols.io.xiifkce | 2021-03-29 03:11:48 | ||
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UC Davis - Laser Capture Microscopy Resource Report Resource Website |
Saivageethi Nuthikattu, Jennifer Rutkowsky | 10.17504/protocols.io.zawf2fe | Mouse Metabolic Phenotyping Centers | To quantify regions or cell type specific alterations in RNA expression. Laser capture microscopy is utilized to isolate region or cell-specific regions in the brain and qualitative real time-PCR (qRT-PCR) is used to assess relative or semi-quantitative RNA expression of genes of interest.Modified from: Laser Capture Microscopy Protocol- Van Winkle Lab | University of California, Davis, University of California, Davis | https://mmpc.org/shared/document.aspx?id=281&docType=Protocol | 1 | 2019 | Saivageethi Nuthikattu, Jennifer Rutkowsky 2019. UC Davis - Laser Capture Microscopy. protocols.io dx.doi.org/10.17504/protocols.io.zawf2fe | 2021-03-29 03:11:45 | ||
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U Michigan - Podocyte Counting And Density Analysis Resource Report Resource Website |
Jeff Hodgin, Jharna Saha | 10.17504/protocols.io.565g9g6 | Mouse Metabolic Phenotyping Centers | This protocol is used to stain and count podocyte nuclei in mouse glomeruli for diabetic nephropathy study. | RRID:AB_2336517 | University of Michigan - Ann Arbor, University of Michigan - Ann Arbor | https://mmpc.org/shared/document.aspx?id=322&docType=Protocol | 2 | 2019 | Jeff Hodgin, Jharna Saha 2019. U Michigan - Podocyte Counting And Density Analysis. protocols.io dx.doi.org/10.17504/protocols.io.565g9g6 | 2021-03-29 03:13:24 | |
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U Mass - Uric Acid Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.x5sfq6e | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum uric acid levels are affected by alterations in systemic protein and nitrogen metabolism. Serum uric acid levels are altered in kidney failure and renal complications of diabetes. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=188&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Uric Acid. protocols.io dx.doi.org/10.17504/protocols.io.x5sfq6e | 2021-03-29 03:13:33 | ||
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U Cinn - Phospholipids Assay Resource Report Resource Website |
Patrick Tso, Dana Lee | 10.17504/protocols.io.xmvfk66 | Mouse Metabolic Phenotyping Centers | Determinations of phospholipids in plasma/serum/lymph will be made using the Wako Phospholipids C enzymatic assay. In this assay, phospholipids in the sample are hydrolyzed ultimately producing a blue pigment. The amount of phospholipids in the sample is determined by measuring the absorbance of the blue color. | University of Cincinnati, University of Cincinnati | https://mmpc.org/shared/document.aspx?id=198&docType=Protocol | 1 | 2019 | Patrick Tso, Dana Lee 2019. U Cinn - Phospholipids Assay. protocols.io dx.doi.org/10.17504/protocols.io.xmvfk66 | 2021-03-29 03:13:37 | ||
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UC Davis - Triglyceride Protocol Resource Report Resource Website |
Peter Havel | 10.17504/protocols.io.yw3fxgn | Mouse Metabolic Phenotyping Centers | Summary: Triglycerides are enzymatically hydrolyzed by lipase to free fatty acids and glycerol. The glycerol is phosphorylated by adenosine triphosphate (ATP) with glycerol kinase (GK) to produce glycerol-3- phosphate and adenosine diphosphate. Glycerol-3-phosphate is oxidized by dihydroxyacetone phosphate (DAP) by glycerolphosphate oxidase producing hydrogen peroxide (H2O2). In a Trinder5 type color reaction catalyzed by peroxidase, the H2O2 reacts with 4-aminoantipyrine (4-AAP) and 3,5-dichloro-2- hydroxybenzene sulfonate (DHBS) to produce a red colored dye. The absorbance of this dye is proportional to the concentration of triglycerides present in the sample. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=91&docType=Protocol | 1 | 2019 | Peter Havel 2019. UC Davis - Triglyceride Protocol. protocols.io dx.doi.org/10.17504/protocols.io.yw3fxgn | 2021-03-29 03:13:35 | ||
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Vandy – Chronic Catheterization of carotid artery and jugular vein Resource Report Resource Website |
Bingle Bracy | 10.17504/protocols.io.zddf226 | Mouse Metabolic Phenotyping Centers | Study of the unstressed mouse requires prior implantation of catheters in the carotid artery and jugular vein. The arterial catheter is used to sample blood and the venous catheter is used as an infusion port. The surgical procedure is performed under sterile conditions in accordance with AAALAC standards. | Vanderbilt University | https://mmpc.org/shared/document.aspx?id=236&docType=Protocol | 1 | 2019 | Bingle Bracy 2019. Vandy – Chronic Catheterization of carotid artery and jugular vein. protocols.io dx.doi.org/10.17504/protocols.io.zddf226 | 2021-03-29 03:13:41 | ||
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UC Davis - Blood Pressure/Heart Rate by Telemetry Resource Report Resource Website |
Lynette Bower | 10.17504/protocols.io.yevfte6 | Mouse Metabolic Phenotyping Centers | Utilizing this telemetry we have the ability to simultaneously measure a pressure signal and biopotential signal (ECG, EEG, EMG) in a single mouse. This can provide a more comprehensive physiological assessment of cardiovascular function, the presence of any damage to the heart, the effects of drugs, specific genes or interventions used to regulate the heart.Modified from: Butz et al. Physiol Genomics. 2001 Mar 8;5(2):89-97. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=131&docType=Protocol | 1 | 2019 | Lynette Bower 2019. UC Davis - Blood Pressure/Heart Rate by Telemetry . protocols.io dx.doi.org/10.17504/protocols.io.yevfte6 | 2021-03-29 03:13:41 | ||
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UC Davis - ß hydroxy butyrate Protocol Resource Report Resource Website |
Peter Havel | 10.17504/protocols.io.ywafxae | Mouse Metabolic Phenotyping Centers | When a sample is mixed with R1, AcAc in the sample is broken down to acetone by AADC. Upon addition of R2, 3-HB in the sample is oxidized in the presence of 3-HBDH and Thio-NAD. This oxidation triggers the cyclic reactions. Since the original AcAc in the sample has been removed, only 3-HB is assayed by measureing the rate of Thio-NADH production spectrophotometrically. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=90&docType=Protocol | 1 | 2019 | Peter Havel 2019. UC Davis - ß hydroxy butyrate Protocol. protocols.io dx.doi.org/10.17504/protocols.io.ywafxae | 2021-03-29 03:13:48 | ||
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UC Davis - Immunohistochemistry BODIPY Resource Report Resource Website |
Jennifer Rutkowsky | 10.17504/protocols.io.56rg9d6 | Mouse Metabolic Phenotyping Centers | BODIPY® 493/503 can be used as a stain for neutral lipids and as a tracer for oil and other nonpolar lipids. It has uses for identifying and quantifying lipid droplets in various cell and tissue type. Abnormal lipid droplet accumulation can be associated with various metabolic disorders such as Diabetes, cardiovascular disease and cognitive impairment. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=254&docType=Protocol | 2 | 2019 | Jennifer Rutkowsky 2019. UC Davis - Immunohistochemistry BODIPY. protocols.io dx.doi.org/10.17504/protocols.io.56rg9d6 | 2021-03-29 03:13:50 | ||
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UC Davis - IN-VIVO Glucose-stimulates Insulin Secretion Test Resource Report Resource Website |
Fawaz G. Haj | 10.17504/protocols.io.yptfvnn | Mouse Metabolic Phenotyping Centers | Summary: An in-vivo glucose-stimulates insulin secretion test is designated to determine alterations in insulin secretion by the pancreas upon a bolus IP Glucose injection. | RRID:AB_2783626 | University of California, Davis | https://mmpc.org/shared/document.aspx?id=86&docType=Protocol | 1 | 2019 | Fawaz G. Haj 2019. UC Davis - IN-VIVO Glucose-stimulates Insulin Secretion Test. protocols.io dx.doi.org/10.17504/protocols.io.yptfvnn | 2021-03-29 03:12:37 | |
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U Mass - Albumin Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.xprfmm6 | Mouse Metabolic Phenotyping Centers | This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Albumin is a major circulating protein in body. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=179&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Albumin. protocols.io dx.doi.org/10.17504/protocols.io.xprfmm6 | 2021-03-29 03:12:38 | ||
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UC Davis - Corticosterone RIA Resource Report Resource Website |
Peter Havel | 10.17504/protocols.io.ygiftue | Mouse Metabolic Phenotyping Centers | The RIA (RadioImmuno Assay) is an assay method used for the quantification of various proteins. A standardized concentration of antibody specific to the analyte of interest is pipetted into test tubes. A standardized concentration of the analyte of interest that is labeled with a radioisotope (usually 125I) is added to the tubes. Standards and samples are pipetted into the tubes and the tubes are incubated. During the incubation period the standardized concentration of labeled analyte and the unknown concentration of analyte in the samples will compete for binding sites on the antibody. After the incubation period a precipitating reagent is added to the tubes and the bound antibodies are precipitated out using the double antibody-polyethyleneglycol precipitation technique. The tubes are centrifuged and the supernatant is aspirated and the precipitate is counted (using a gamma counter if 125I is used). If there is a low concentration of the analyte of interest in the samples, the labeled analyte will have a higher probability for binding to the antibody and thus there will be a higher count in the precipitate. If there is a high concentration of the analyte of interest in the samples there will be a lower count of labeled analyte in the precipitate. The counts and known concentrations of the standards are used to generate a standard curve, and the counts of the samples are used to interpolate quantitative concentrations for the analyte of interest from the standard curve. | RRID:AB_2783720 | University of California, Davis | https://mmpc.org/shared/document.aspx?id=265&docType=Protocol | 1 | 2019 | Peter Havel 2019. UC Davis - Corticosterone RIA. protocols.io dx.doi.org/10.17504/protocols.io.ygiftue | 2021-03-29 03:12:40 | |
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U Mass - Chronic drug delivery Resource Report Resource Website |
Jason Kim | 10.17504/protocols.io.xuefnte | Mouse Metabolic Phenotyping Centers | A subcutaneous or intraperitoneal implantation of Alzet osmotic pump is used to chronically administer selected drug in mice. Chronic drug delivery may be used to examine intermediate to long-term effects of selected drug on obesity, insulin resistance, and metabolism. | University of Massachusetts | https://mmpc.org/shared/document.aspx?id=151&docType=Protocol | 1 | 2019 | Jason Kim 2019. U Mass - Chronic drug delivery. protocols.io dx.doi.org/10.17504/protocols.io.xuefnte | 2021-03-29 03:12:41 | ||
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Vandy - Modified Roux-en-Y Gastric Bypass in Mice Resource Report Resource Website |
Teri Stevenson, Vance L. Albaugh | 10.17504/protocols.io.zchf2t6 | Mouse Metabolic Phenotyping Centers | This is the protocol for the modified gastric bypass in the mouse. In this procedure the stomach is bypassed and the food flows through the bypass arm directly into the jejunum. | Vanderbilt University, Vanderbilt University | https://mmpc.org/shared/document.aspx?id=294&docType=Protocol | 1 | 2019 | Teri Stevenson, Vance L. Albaugh 2019. Vandy - Modified Roux-en-Y Gastric Bypass in Mice. protocols.io dx.doi.org/10.17504/protocols.io.zchf2t6 | 2021-03-29 03:12:46 | ||
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UC Davis - Mouse Model Creation Resource Report Resource Website |
Kent Lloyd | 10.17504/protocols.io.56gg9bw | Mouse Metabolic Phenotyping Centers | This is a generic protocol for making genetically-altered mouse models for research. Investigators who do not have a mutant mouse to send for testing at the MMPC @ UC Davis can ask for the mutant mouse to be derived by the MMPC @ UC Davis, through the UC Davis Mouse Biology Program. Genetically altered mice can be made on most genetic backgrounds, such as C57BL/6, FVB/N, B6D2F1 and other hybrids, and others. Transgenic (random genomic integration of expression-competent transgenes) and gene targeted (specific insertion and/or replacement of an endogenous genetic locus) mutant mice can generated. “Knockout” mice can be made so as to delete endogenous gene expression ubiquitously and conditionally in either a specific cell or tissue type (spatial conditional) and/or at a specific age (temporal conditional). Numerous technologies are available for the derivation of mutant mice, including molecular construct design, gene targeted in embryonic stem (ES) cells, pronuclear microinjection of transgenic constructs into zygotes, ES cell microinjection into blastocysts, genome editing in ES cells and zygotes (e.g., TALENs, CRISPR/Cas9). Mutant mice can be genotype confirmed (see MMPC item D2004) and custom bred to generate cohorts of male and/or female mice for phenotyping at the MMPC @ UC Davis. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=261&docType=Protocol | 2 | 2019 | Kent Lloyd 2019. UC Davis - Mouse Model Creation. protocols.io dx.doi.org/10.17504/protocols.io.56gg9bw | 2021-03-29 03:12:47 | ||
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UC Davis - Digestible Energy by Bomb Calorimetry Resource Report Resource Website |
Trina Knotts | 10.17504/protocols.io.ygkftuw | Mouse Metabolic Phenotyping Centers | Changes in fecal energy loss can result in differences in net energy balance and feed efficiency, and may result from, e.g., alterations in bile acid production, secretion or re-uptake, and changes in activities of enzymes involved in macronutrient digestion or uptake (e.g., pancreatic lipase). This assay will be coupled to food intake determinations and will involve fecal collection and feces weight measures for 48h, which can be coordinated with the meal pattern studies (catalog item D4003) or indirect calorimetry (catalog item D4007). Diet and fecal energy content will be measured by bomb calorimetry and the following reported: fecal energy density, total fecal energy loss/day, and fecal energy loss as a percent of energy intake. Alternatively, fecal samples and diet samples can be examined without food intake measurements. In this case, only diet and fecal energy density will be reported. Additional tests can be done through proximate analysis (D4005- Digestible Energy by Proximate Analysis) to determine protein or fat absorption. | University of California, Davis | https://mmpc.org/shared/document.aspx?id=286&docType=Protocol | 1 | 2019 | Trina Knotts 2019. UC Davis - Digestible Energy by Bomb Calorimetry. protocols.io dx.doi.org/10.17504/protocols.io.ygkftuw | 2021-03-29 03:12:48 | ||
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Vandy - Mouse Myocardial Infarction Resource Report Resource Website |
Lin Zhong, Jeffrey Rottman, Chee Lim | 10.17504/protocols.io.6xghfjw | Mouse Metabolic Phenotyping Centers | Summary:The most common cause of cardiovascular mortality in man is the outcome from myocardial infarction. Accordingly, it is necesarry to study the corresponding process of heart injury recovery in many mouse models relevant to human cardiovascular disease. This protocol describes the surgical induction of myocardial injury via 1) permanent ligation of a coronary artery in the heart (myocardial infarction), 2) cryoinjury, and 3) transient occlusion of a coronary artery followed by reperfusion (ischemia-reperfusion injury). | Vanderbilt University, Vanderbilt University, Vanderbilt University | https://mmpc.org/shared/document.aspx?id=227&docType=Protocol | 2 | 2019 | Lin Zhong, Jeffrey Rottman, Chee Lim 2019. Vandy - Mouse Myocardial Infarction. protocols.io dx.doi.org/10.17504/protocols.io.6xghfjw | 2021-03-29 03:12:53 | ||
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Yale - Blood Urea Nitrogen Resource Report Resource Website |
John Stack, Gary Cline | 10.17504/protocols.io.y3qfymw | Mouse Metabolic Phenotyping Centers | Procedure used to measure the concentration of Blood Urea Nitrogen(BUN) in blood, plasma, and serum. Urea is determined by the enzymatically coupled reactions of urease (to form ammonia) and glutamate dehydrogenase (conversion of ammonia and glutamate to glutamine with oxidation of NADH to NAD). The rate of NAD formation is monitored by the change in absorbance at 340 nm. | Yale University, Yale University | https://mmpc.org/shared/document.aspx?id=203&docType=Protocol | 1 | 2019 | John Stack, Gary Cline 2019. Yale - Blood Urea Nitrogen. protocols.io dx.doi.org/10.17504/protocols.io.y3qfymw | 2021-03-29 03:12:52 | ||
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Yale - Beta hydroxybutyrate (Cobas) Resource Report Resource Website |
Gary Cline, John Stack | 10.17504/protocols.io.y2bfyan | Mouse Metabolic Phenotyping Centers | Procedure used to determine the concentration of β-Hydroxybutyrate in blood, serum, and plasma. β-Hydroxybutyrate is measured by the oxidation to acetoacetate, followed by reduction of an indicator dye (monitored at 505 nm) by NADH. | Yale University, Yale University | https://mmpc.org/shared/document.aspx?id=215&docType=Protocol | 1 | 2019 | Gary Cline, John Stack 2019. Yale - Beta hydroxybutyrate (Cobas). protocols.io dx.doi.org/10.17504/protocols.io.y2bfyan | 2021-03-29 03:14:25 |
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