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Authors: Sam Li
Group: BioLegend
Proper citation: Sam Li 2019. True-Nuclear™ Transcription Factor Staining Protocol for 96-Well, U-Bottom Plate. protocols.io dx.doi.org/10.17504/protocols.io.bacriav6 Copy
Authors: Sam Li
Group: BioLegend
Summary: The kit is designed for the isolation of human memory CD8 T cells from PBMC. Target cells are depleted by incubating the sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.
Proper citation: Sam Li 2017. MojoSort™ Human CD8 Memory T Cells Isolation Kit Protocol. protocols.io dx.doi.org/10.17504/protocols.io.ihjcb4n Copy
Authors: Kelsey Miller
Group: BioLegend
Summary: Anti-Neu5Gc Antibody Kit Protocols for Flow Cytometry, ELISA, and Western blot.
Proper citation: Kelsey Miller 2016. Anti-Neu5Gc Antibody Kit Protocols. protocols.io dx.doi.org/10.17504/protocols.io.e25bgg6 Copy
Authors: Kelsey Miller
Group: BioLegend
Summary: The Anti-Neu5Gc Antibody Kit contains the essential monospecific polyclonal chicken IgY antibody, along with a negative control primary antibody to detect the presence of Neu5Gc on glycoconjugates by Western blot (WB). Samples to be evaluated are first subjected to SDS-PAGE, followed by transfer to a nitrocellulose or polyvinylidenedifluoride (PVDF) membrane. The membrane is then incubated with affinity-purified polyclonal anti-Neu5Gc to determine the presence of Neu5Gc on the protein of interest.The antibody provided in this kit has been shown to identify as little as 5 pmol of Neu5Gc per ug glycoprotein, which is at or below the current detection limit for conventional analysis by acid release, purification, DMB derivatization, HPLC, and electrospray mass-spectrometry. The Western blot provides additional information in that it confirms that Neu5G is directly linked to the glycoprotein of interest rather than to an accompanying sample component.
Proper citation: Kelsey Miller 2016. Anti-Neu5Gc Antibody Kit Protocol - Western blot. protocols.io dx.doi.org/10.17504/protocols.io.e28bghw Copy
Authors: Sam Li
Group: BioLegend
Proper citation: Sam Li 2019. Western Blotting Protocol. protocols.io dx.doi.org/10.17504/protocols.io.98mh9u6 Copy
Authors: Sam Li
Group: BioLegend
Summary: Product description and procedure summary:
If your target cells are the labeled cells (the positive fraction), use the Streptavidin Nanobeads Protocol – Positive Selection. If your target cells are the unlabeled cells (negative fraction), use the Streptavidin Nanobeads Protocol - Negative Selection.
Target cells are isolated by incubating your sample with a Biotin-conjugated antibody or antibody cocktail, followed by incubation with magnetic Streptavidin Nanobeads (Cat. No. 480015/480016). The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.
Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14 mL tubes and Magnet, and place the tube in the magnet for 10 minutes.
Proper citation: Sam Li 2019. MojoSort™ Streptavidin Nanobeads Protocol - Negative Selection. protocols.io dx.doi.org/10.17504/protocols.io.7u8hnzw Copy
Authors: Sam Li
Group: BioLegend
Summary: Product description and procedure summary: Target cells are either selected or depleted by incubating the sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads (Cat. No. 480015/480016). The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. If these are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.Note: This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. Scale up volumes if using 14mL tubes and Magnet, and place the tube in the magnet for 10 minutes.
Proper citation: Sam Li 2019. MojoSort™ Selection Kits Protocol - 4. protocols.io dx.doi.org/10.17504/protocols.io.7yyhpxw Copy
Authors: BioLegend, Inc.
Group: BioLegend
Proper citation: BioLegend, Inc. 2016. Intracellular Flow Cytometry Staining Protocol: For the Detection of Intracellular Cytokines and Other Intracellular Targets. protocols.io dx.doi.org/10.17504/protocols.io.ez2bf8e Copy
Authors: Kelsey Knight
Group: BioLegend
Proper citation: Kelsey Knight 2018. T Cell Activation with anti-CD3 Antibodies Protocol - Human. protocols.io dx.doi.org/10.17504/protocols.io.tkbeksn Copy
Authors: BioLegend, Inc.
Group: BioLegend
Proper citation: BioLegend, Inc. 2016. Immunofluorescent Staining of Whole Blood. protocols.io dx.doi.org/10.17504/protocols.io.exqbfmw Copy
Authors: BioLegend, Inc.
Group: BioLegend
Proper citation: BioLegend, Inc. 2016. Direct-Blot™ Western Blotting Protocol. protocols.io dx.doi.org/10.17504/protocols.io.e2gbgbw Copy
Authors: BioLegend, Inc.
Group: BioLegend
Proper citation: BioLegend, Inc. 2016. True-Nuclear™ Transcription Factor Staining Protocol for 5 mL Tubes. protocols.io dx.doi.org/10.17504/protocols.io.gq4bvyw Copy
Authors: Kelsey Miller
Group: BioLegend
Proper citation: Kelsey Miller 2016. Intracellular Staining With True-Phos™ Perm Buffer in Cell Suspensions. protocols.io dx.doi.org/10.17504/protocols.io.e2fbgbn Copy
Authors: Sam Li
Group: BioLegend
Summary: Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.The following protocol describes surface protein staining with TotalSeq™–B and TotalSeq™–C antibodies and/or hashtag antibodies, to enable protein detection in addition to Single Cell 3’ v3 and Single Cell V(D)J Feature Barcoding technology from 10x Genomics.Please read the entire protocol before starting the experiments.
Proper citation: Sam Li 2019. TotalSeq™-B or -C with 10x Feature Barcoding Technology. protocols.io dx.doi.org/10.17504/protocols.io.8v5hw86 Copy
Authors: Sam Li
Group: BioLegend
Proper citation: Sam Li 2019. Ki-67 Staining Protocol. protocols.io dx.doi.org/10.17504/protocols.io.bac2iaye Copy
Authors: Kelsey Miller
Group: BioLegend
Summary: This procedure is optimized for the isolation of 107 to 1 x 108 cells per tube. If working with fewer than 107 cells, keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water.Product description and procedure summary:Non Neutrophil cells are depleted by incubating the sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched cells are collected. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.
Proper citation: Kelsey Miller 2016. MojoSort™ Mouse Neutrophil Isolation Kit Protocol. protocols.io dx.doi.org/10.17504/protocols.io.fxnbpme Copy
Authors: Sam Li
Group: BioLegend
Proper citation: Sam Li 2019. Immunohistochemistry Protocol for Ultra Streptavidin Detection Kits (USA). protocols.io dx.doi.org/10.17504/protocols.io.95rh856 Copy
Authors: Sam Li
Group: BioLegend
Summary: BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed.
Proper citation: Sam Li 2019. MojoSort™ Human CD14+ Monocyte Isolation Kit Column Protocol. protocols.io dx.doi.org/10.17504/protocols.io.7bghijw Copy
Authors: Sam Li
Group: BioLegend
Summary: BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody than with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:3 to 1:20 for the Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed.
Proper citation: Sam Li 2019. MojoSort™ Selection Kits Column Protocol - 3. protocols.io dx.doi.org/10.17504/protocols.io.7tvhnn6 Copy
Authors: Sam Li
Group: BioLegend
Summary: BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer.Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used. Please contact BioLegend Technical Service ([email protected]) if further assistance is needed.
Proper citation: Sam Li 2019. MojoSort™ Isolation Kits Column Protocol - 1. protocols.io dx.doi.org/10.17504/protocols.io.7bahiie Copy
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