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Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2014. Mixture for M0290 CIP. protocols.io dx.doi.org/10.17504/protocols.io.chrt55 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocols is to be performed with the Quick Ligation Reaction Buffer. Please see the NEB website for more information.
Proper citation: New England Biolabs 2017. Quick Ligation Protocol (M2200). protocols.io dx.doi.org/10.17504/protocols.io.iqvcdw6 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. PNGase F Mixture 2. protocols.io dx.doi.org/10.17504/protocols.io.cqhvt5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of RNA into high quality directional (strand-specific) libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow has minimal hands-on time and is compatible with poly(A) mRNA enrichment and rRNA depletion methods.
Proper citation: New England Biolabs 2018. Protocol for use with FFPE RNA, NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765). protocols.io dx.doi.org/10.17504/protocols.io.tumenu6 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA. This protocol describes how to determine genome targeting efficiency by digesting annealed PCR products with T7 Endonuclease I. In the first step PCR products are produced from the genomic DNA of cells whose genomes were targeted using Cas9, TALEN, ZFN etc. In the second step, the PCR products are annealed and digested with T7 Endonuclease I. Fragments are analyzed to determine the efficiency of genome targeting.
Proper citation: New England Biolabs 2015. Determining Genome Targeting Efficiency using T7 Endonuclease I (M0302). protocols.io dx.doi.org/10.17504/protocols.io.cqpvvm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II FS DNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of DNA into high quality libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow also has minimal hands-on time.Note: The Ultra II FS Kit is not compatible with bisulfite conversion workflows.
Proper citation: New England Biolabs 2021. E7805 NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® Protocol for Large Fragment Sizes (> 550 bp) (Chapter 3). protocols.io dx.doi.org/10.17504/protocols.io.n2idgce Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for the removal of single-stranded extension using Mung Bean Nuclease (M0250).
Proper citation: New England Biolabs 2015. Removal of Single-Stranded Extension using Mung Bean Nuclease (M0250). protocols.io dx.doi.org/10.17504/protocols.io.crhv35 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: PCR Protocol for Taq DNA Polymerase with ThermoPol® Buffer (M0267)
Proper citation: New England Biolabs 2015. PCR with Taq and ThermoPol® Buffer (M0267). protocols.io dx.doi.org/10.17504/protocols.io.cifubm Copy
Authors: New England Biolabs, Menna Teffera
Group: New England Biolabs (NEB)
Summary: The NEBNext dA-Tailing Module has been optimized to efficiently incorporate a non-templated dAMP on the 3 ́ end of a blunt DNA fragment (1). 3 ́-dA DNA tailing prevents concadamer formation during subsequent ligation steps.
DNA tailed with the NEBNext dA-Tailing module may be ligated to adaptors or cloning vectors with complementary dT overhangs. The NEBNext dA-Tailing Module is provided as a master mix to maximize efficiency and convenience in DNA sample preparation workflows.
The NEBNext dA-Tailing Module has been validated by sequencing with the Il- lumina Genome Analyzer II (Illumina, Inc.) in conjunction with the NEBNext End Repair Module, NEBNext Quick Ligation Module and Phusion® High-Fidelity PCR Master Mix.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
Proper citation: New England Biolabs, Menna Teffera 2019. NEBNext dA-Tailing Module (NEB #E6053). protocols.io dx.doi.org/10.17504/protocols.io.4t3gwqn Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the protocol for dephosphorylation of 5'-ends of DNA using AnP (Antarctic Phosphatase - M0289).
Proper citation: New England Biolabs 2014. Dephosphorylation using AnP (M0289). protocols.io dx.doi.org/10.17504/protocols.io.cdns5d Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Rapid and irreversible heat inactivation eliminates unwanted activityImproved storage stability versus native enzymeFaster reaction setup (no supplemental additives like zinc required) and shorter incubation timeFlexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)Less enzyme required (high specific activity), resulting in a lower cost per reactionNo need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysisRecombinant for purity, consistency and value
Proper citation: New England Biolabs 2020. Enzymatic PCR Cleanup Protocol (NEB #M0525). protocols.io dx.doi.org/10.17504/protocols.io.7r4hm8w Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. M0371 no RE mixture. protocols.io dx.doi.org/10.17504/protocols.io.crmv45 Copy
Authors: Lauren Moran, New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) depletes both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochodrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g. FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications.
Proper citation: Lauren Moran, New England Biolabs 2018. Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) E6310 (E7760). protocols.io dx.doi.org/10.17504/protocols.io.sxuefnw Copy
Authors: New England Biolabs
Proper citation: New England Biolabs 2015. Mixture for E5510 Gibson. protocols.io dx.doi.org/10.17504/protocols.io.cinudd Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II DNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of DNA into high quality libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow also has minimal hands-on time.
Proper citation: New England Biolabs 2018. Ultra II DNA Library Prep Kit for Illumina E7645/E7103. protocols.io dx.doi.org/10.17504/protocols.io.mc5c2y6 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocol consists of an IgG purification step followed by covalent cross-linking of the IgG to the Protein A/G solid support. For IgG that has been previously purified, proceed directly to the cross-linking protocol.
Proper citation: New England Biolabs 2015. Cross-linking of IgG to Protein A or G Beads (S1425/S1430). protocols.io dx.doi.org/10.17504/protocols.io.crdv25 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Mixture for M0493 Q5 PCR. protocols.io dx.doi.org/10.17504/protocols.io.cjsund Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the Sequential Double Digest Protocol with Standard Restriction Enzymes. If there is no buffer in which the two enzymes exhibit > 50% activity, this sequential digest can be performed.
Proper citation: New England Biolabs 2014. Sequential Double Digest. protocols.io dx.doi.org/10.17504/protocols.io.cjmuk5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Protocol for Dephosphorylation of 5´-ends of DNA using CIP in Restriction Enzyme Reaction. Uses the Calf Intestinal Alkaline Phosphatase (CIP - M0290)
Proper citation: New England Biolabs 2017. Dephosphorylation of 5´-ends of DNA using CIP (M0290). protocols.io dx.doi.org/10.17504/protocols.io.iqwcdxe Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Quick Protocol for Oligonucleotide Cleanup Using the Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030)
Proper citation: New England Biolabs 2018. Quick Protocol for Oligonucleotide Cleanup Using the Monarch® PCR & DNA Cleanup Kit (5 ?g) (NEB #T1030). protocols.io dx.doi.org/10.17504/protocols.io.nyfdftn Copy
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