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Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This protocol is for the "First Strand cDNA Synthesis Kit using ProtoScript II Reverse Transcriptase (M0368)".
Proper citation: New England Biolabs 2014. First Strand cDNA Synthesis (M0368). protocols.io https://dx.doi.org/10.17504/protocols.io.chtt6m Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Protocol for Dephosphorylation of 5´-ends of DNA using CIP in Restriction Enzyme Reaction. Uses the Calf Intestinal Alkaline Phosphatase (CIP - M0290)
Proper citation: New England Biolabs 2017. Dephosphorylation of 5´-ends of DNA using CIP (M0290). protocols.io https://dx.doi.org/10.17504/protocols.io.iqwcdxe Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Mixture for M0493 Q5 PCR. protocols.io https://dx.doi.org/10.17504/protocols.io.cjsund Copy
Authors: New England Biolabs
Group: Grupo de Estudos de Leveduras Cervejeiras
Proper citation: New England Biolabs 2018. SOC Media. protocols.io https://dx.doi.org/10.17504/protocols.io.pngdmbw Copy
Authors: New England Biolabs
Group: Coronavirus Method Development Community
Summary: This protocol details methods for the NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®), NEB #E7660S/L 24/96 reactions.
Proper citation: New England Biolabs 2021. NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®) E7660. protocols.io https://dx.doi.org/10.17504/protocols.io.btcenite Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the correct protocol if you are using the C2987I cells. If you are using the C2987H cells, please refer to this protocol.
Proper citation: New England Biolabs 2014. High Efficiency Transformation Protocol (C2987I). protocols.io https://dx.doi.org/10.17504/protocols.io.chht35 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Exo-CIP™ Rapid PCR Cleanup KitRapidly degrade residual PCR primers and dephosphorylate excess dNTPs after amplificationReaction complete in 4 minutesThermolabile formulation can be heat inactivated in 1 minute at 80°CPCR product can be used directly in downstream applicationsCompatible with commonly-used reaction buffers
Proper citation: New England Biolabs 2020. Protocol for Exo-CIP™ Rapid PCR Cleanup (#E1050). protocols.io https://dx.doi.org/10.17504/protocols.io.8yahxse Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the reaction for the "Ligation Protocol with T4 DNA Ligase"
Proper citation: New England Biolabs 2017. T4 DNA Ligase Reaction. protocols.io https://dx.doi.org/10.17504/protocols.io.irwcd7e Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2014. Single-temp DD Reaction. protocols.io https://dx.doi.org/10.17504/protocols.io.cjjukm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBExpress MBP Fusion and Purification System takes advantage of the strong Ptac promoter and the translation initiation signals of maltose binding protein (MBP) to enhance solubility and expression levels of a desired protein inE. coli. The resulting product is an MBP fusion protein, which is then purified by affinity chromatography.ReliableE. coli expression: substantial yields (up to 100 mg/L)Fusion to MBP has been shown to enhance the solubility of proteins expressed inE. coli(1)Two-step purification: amylose elution followed by TEV Protease cleavage and Ni resin isolation results in a highly pure tag-free target proteinGentle elution with maltose; no detergents or harsh denaturants required
Proper citation: New England Biolabs 2020. NEBExpress MBP Fusion and Purification System (NEB #E8201). protocols.io https://dx.doi.org/10.17504/protocols.io.bfayjifw Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Mixture for M0530 Phusion PCR. protocols.io https://dx.doi.org/10.17504/protocols.io.cgztx5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: This is the PCR protocol for Phusion® High-Fidelity DNA Polymerase (M0530)
Proper citation: New England Biolabs 2017. PCR Protocol for Phusion® High-Fidelity DNA Polymerase (M0530). protocols.io https://dx.doi.org/10.17504/protocols.io.irrcd56 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of RNA into high quality directional (strand-specific) libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow has minimal hands-on time and is compatible with poly(A) mRNA enrichment and rRNA depletion methods.
Proper citation: New England Biolabs 2018. Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765). protocols.io https://dx.doi.org/10.17504/protocols.io.tunenve Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. PNGase F Non-Denaturing Mixture. protocols.io https://dx.doi.org/10.17504/protocols.io.cqjvum Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. Mixture for M0491 Q5 PCR. protocols.io https://dx.doi.org/10.17504/protocols.io.cieubd Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The protocol described below will enable degradation of up to 20 pmol of a 25 nt ssDNA (~ 200 ng). In order to degrade larger amounts of ssDNA or ssDNAs longer than 25 nt, we recommend adding more enzyme instead of extending the reaction time. Users should note that ssDNAs longer than 25 nt may form secondary structures that hinder Thermolabile Exonuclease I activity.
Proper citation: New England Biolabs 2020. Protocol for removing ssDNA from dsDNA or RNA Samples (NEB #M0568). protocols.io https://dx.doi.org/10.17504/protocols.io.7ryhm7w Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: Ligation protocol for the PCR Cloning Kit (E1202)
Proper citation: New England Biolabs 2015. Ligation Protocol for NEB PCR Cloning Kit (E1202). protocols.io https://dx.doi.org/10.17504/protocols.io.cp5vq5 Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Proper citation: New England Biolabs 2015. T4 PNK Minus Reaction. protocols.io https://dx.doi.org/10.17504/protocols.io.cp3vqm Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The following is a "typical" restriction endonuclease reaction. Please see the "guidelines" tab below for the NEB tips on optimizing restriction digests.
Proper citation: New England Biolabs 2014. Restriction Digest. protocols.io https://dx.doi.org/10.17504/protocols.io.chkt4v Copy
Authors: New England Biolabs
Group: New England Biolabs (NEB)
Summary: The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina contains the enzymes and buffers required to convert a broad range of input amounts of RNA into high quality directional (strand-specific) libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow has minimal hands-on time and is compatible with poly(A) mRNA enrichment and rRNA depletion methods.
Proper citation: New England Biolabs 2018. Protocol for use with rRNA Depleted FFPE RNA and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765). protocols.io https://dx.doi.org/10.17504/protocols.io.ubaesie Copy
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