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Name Authors DOI Group Summary Associated Publications RRIDs used Affiliations External URL Version Publication Date Proper Citation Record Last Update
Gamma-diversity partitioning of gobiid fishes (Teleostei: Gobiidae) ensemble along of Eastern Tropical Pacific: biological inventory, latitudinal variation and species turnover
 
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Omar Valencia 10.17504/protocols.io.s3iegke Results of the additive and multiplicative diversity partitioning of gobiid fishes Valencia-Méndez O, Rodríguez-Zaragoza FA, Calderon-Aguilera LE, Domínguez-Domínguez O, López-Pérez A (2018) Gamma-diversity partitioning of gobiid fishes (Teleostei: Gobiidae) ensemble along of Eastern Tropical Pacific: Biological inventory, latitudinal variation and species turnover. PLoS ONE 13(8): e0202863. doi: 10.1371/journal.pone.0202863 Universidad Autónoma Metropolitana - Iztapalapa https://doi.org/10.1371/journal.pone.0202863 2 2018 Omar Valencia 2018. Gamma-diversity partitioning of gobiid fishes (Teleostei: Gobiidae) ensemble along of Eastern Tropical Pacific: biological inventory, latitudinal variation and species turnover. protocols.io dx.doi.org/10.17504/protocols.io.s3iegke 2021-03-29 03:10:12
TissueCyte Specimen Embedding Acrylamide Coembedding
 
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Allen Institute for Brain Science 10.17504/protocols.io.bdpvi5n6 BICCN, Allen Institute for Brain Science This protocol describes the preparation of a fixed mouse brain specimen bonded to an agarose block for blockface imaging and sectioning using the TissueCyte 1000 system.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Allen Institute 2 2020 Allen Institute for Brain Science 2020. TissueCyte Specimen Embedding Acrylamide Coembedding. protocols.io dx.doi.org/10.17504/protocols.io.bdpvi5n6 2021-03-29 03:10:13
PROTOCCOLS FOR PREPARATION OF BLOOD FILMS, REPORTING OF MALARIA PARASITES, BLOOD GROUPING AND QUESTIONNAIRE DESIGN
 
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Kwame Adusei, Alex Owusu-Ofori 10.17504/protocols.io.pq6dmze Transfusion-transmitted malaria, Donor prevalence and health worker knowledge and practices. Protocols for preparation of blood smears (Thick and thin films), reporting of malaria parasites, blood grouping and design of questionnaires. Department of Medical Laboratory Service, School of Allied Health Sciences, Kwame Nkrumah University of Science and Technology Kumasi, Ghana ,Tel: +233 501365180, Email: [email protected], Department of Clinical Microbiology School of Medical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana. Tel: +233 209149370, Email: [email protected] 1 2018 Kwame Adusei, Alex Owusu-Ofori 2018. PROTOCCOLS FOR PREPARATION OF BLOOD FILMS, REPORTING OF MALARIA PARASITES, BLOOD GROUPING AND QUESTIONNAIRE DESIGN. protocols.io dx.doi.org/10.17504/protocols.io.pq6dmze 2021-03-29 03:10:13
Coccidioides_soil_habitat_model
 
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Robert Dobos 10.17504/protocols.io.bh6gj9bw Dobos RR, Benedict K, Jackson BR, McCotter OZ (2021) Using soil survey data to model potential Coccidioides soil habitat and inform Valley fever epidemiology. PLoS ONE 16(2): e0247263. doi: 10.1371/journal.pone.0247263 Kaitlin Benedict, Brendan R. Jackson, Orion Z. McCotter https://doi.org/10.1371/journal.pone.0247263 1 2020 Robert Dobos 2020. Coccidioides_soil_habitat_model. protocols.io dx.doi.org/10.17504/protocols.io.bh6gj9bw 2021-03-29 03:10:13
Gibson Assembly (NEBuilder)
 
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Snehadri Sinha 10.17504/protocols.io.gzebx3e Aalto-Helsinki 2016 Gibson assembly using NEBuilder HiFi DNA assembly kit. Specification of the original NEB protocol. 1 2017 Snehadri Sinha 2017. Gibson Assembly (NEBuilder). protocols.io dx.doi.org/10.17504/protocols.io.gzebx3e 2021-03-29 03:10:07
RA STRAIN SUPPLEMENT
 
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Manuel Richter 10.17504/protocols.io.8wjhxcn RA STRAIN SUPPLEMENT University of Giessen 1 2019 Manuel Richter 2019. RA STRAIN SUPPLEMENT. protocols.io dx.doi.org/10.17504/protocols.io.8wjhxcn 2021-03-29 03:10:07
Ultrasonographic measurements of the right femoral vein cross-sectional area
 
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Dorota Czyzewska1*, Andrzej Ustymowicz2, Radoslaw Kowalewski3¶, Anna Zurada4¶, Jaroslaw Krejza5 10.17504/protocols.io.iq5cdy6 Exclusion criteria: lower limb varices, history of leg surgery, trauma, infection, or thromboembolic events.Ultrasound examinations are performed in B-mode technique, equipped with a linear broadband (7.5-14-MHz) transducer. The right femoral vein and the inguinal ligament are examined in a supine position. The inguinal ligament is identified along its longitudinal axis. The measurements levels are identified as follow:-    proximal level: 20 mm caudally to the inguinal ligament identified along the femoral artery imaged with US-    distal level: 20 mm caudally to the inguinal crease visually identified and along the femoral artery imaged with USThe cross-sectional area (CSA) of the right femoral vein is examined at both levels in transverse plane and is measured automatically after manual tracing of the vessel on a static image in three leg positions: abduction, abduction + external rotation, abduction + external rotation + 90º knee flexion/frog-leg. Attention must be taken to avoid any compression or displacement of the examined vein. Czyzewska D, Ustymowicz A, Kowalewski R, Zurada A, Krejza J (2017) Cross-sectional area of the femoral vein varies with leg position and distance from the inguinal ligament. PLoS ONE 12(8): e0182623. doi: 10.1371/journal.pone.0182623 1First Department of Radiology, Maria Sklodowska-Curie Memorial Cancer Center, Institute of Oncology, Warsaw, Poland 2Department of Radiology, Medical University of Bialystok, Bialystok, Poland 3Department of Vascular Surgery and Transplantation, Medical University of Bialystok, Bialystok, Poland 4Department of Anatomy, Faculty of Medicine University of Varmia and Mazury in Olsztyn, Olsztyn, Poland 5Institute of Innovative Medicine, Advanced Biomedical Image Laboratory, Bialystok, Poland., 1First Department of Radiology, Maria Sklodowska-Curie Memorial Cancer Center, Institute of Oncology, Warsaw, Poland 2Department of Radiology, Medical University of Bialystok, Bialystok, Poland 3Department of Vascular Surgery and Transplantation, Medical University of Bialystok, Bialystok, Poland 4Department of Anatomy, Faculty of Medicine University of Varmia and Mazury in Olsztyn, Olsztyn, Poland 5Institute of Innovative Medicine, Advanced Biomedical Image Laboratory, Bialystok, Poland., 1First Department of Radiology, Maria Sklodowska-Curie Memorial Cancer Center, Institute of Oncology, Warsaw, Poland 2Department of Radiology, Medical University of Bialystok, Bialystok, Poland 3Department of Vascular Surgery and Transplantation, Medical University of Bialystok, Bialystok, Poland 4Department of Anatomy, Faculty of Medicine University of Varmia and Mazury in Olsztyn, Olsztyn, Poland 5Institute of Innovative Medicine, Advanced Biomedical Image Laboratory, Bialystok, Poland., 1First Department of Radiology, Maria Sklodowska-Curie Memorial Cancer Center, Institute of Oncology, Warsaw, Poland 2Department of Radiology, Medical University of Bialystok, Bialystok, Poland 3Department of Vascular Surgery and Transplantation, Medical University of Bialystok, Bialystok, Poland 4Department of Anatomy, Faculty of Medicine University of Varmia and Mazury in Olsztyn, Olsztyn, Poland 5Institute of Innovative Medicine, Advanced Biomedical Image Laboratory, Bialystok, Poland., 1First Department of Radiology, Maria Sklodowska-Curie Memorial Cancer Center, Institute of Oncology, Warsaw, Poland 2Department of Radiology, Medical University of Bialystok, Bialystok, Poland 3Department of Vascular Surgery and Transplantation, Medical University of Bialystok, Bialystok, Poland 4Department of Anatomy, Faculty of Medicine University of Varmia and Mazury in Olsztyn, Olsztyn, Poland 5Institute of Innovative Medicine, Advanced Biomedical Image Laboratory, Bialystok, Poland. https://doi.org/10.1371/journal.pone.0182623 1 2017 Dorota Czyzewska1*, Andrzej Ustymowicz2, Radoslaw Kowalewski3¶, Anna Zurada4¶, Jaroslaw Krejza5 2017. Ultrasonographic measurements of the right femoral vein cross-sectional area. protocols.io dx.doi.org/10.17504/protocols.io.iq5cdy6 2021-03-29 03:10:08
2XYT Medium (Version 2-0) Haseloff Lab
 
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Fernando Guzman Chavez 10.17504/protocols.io.bigekbte Work instruction for 2xYT and 2xYTG medium preparationFollowing this recipe, you will obtain 1L of 2XYT medium2X YTis a standard growth medium used to cultivateE. coli. In cell-free system, this medium is commonly used to growth the cells. .justify:after { content: ""; display:inline-block; width: 100%; } .justify:after { content: ""; display:inline-block; width: 100%; } NOTE :The protocol described here is an adaptation from these papers:Adam D. Silverman, Nancy Kelley-Loughnane, Julius B. Lucks, and Michael C. Jewett (2019).Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry. ACS Synthetic Biology, 403-414. DOI: 10.1021/acssynbio.8b00430.Andriy Didovyk, Taishi Tonooka, Lev Tsimring, and Jeff Hasty. (2017).Rapid and Scalable Preparation of Bacterial Lysates for Cell-Free Gene Expression. ACS Synthetic Biology, 2198-2208. DOI: 10.1021/acssynbio.7b00253. University of Cambridge 1 2020 Fernando Guzman Chavez 2020. 2XYT Medium (Version 2-0) Haseloff Lab. protocols.io dx.doi.org/10.17504/protocols.io.bigekbte 2021-03-29 03:10:08
CD200 and CD200R levels in psoriasis patients
 
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Carmen Farid, Aisha A. Mahmoud, Hanaa M. Donia, Hafsa M. Ghatesh 10.17504/protocols.io.6zihf4e Serum soluble CD200 levels in psoriasis patients was measured by ELISA (Human CD200 PicoKine™ ELISA Kit, Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0993)), and CD200R expression level on both peripheral blood monocytes and lymphocytes were measured by flowcytometry using human CD200 R1 conjugated antibody(Human CD200 R1 Fluorescein-conjugated Antibody, Bio-Techne,Minneapolis, Minnesota, USA, Catalog # FAB3414F)). Cells were analyzed on a FACSCalibur flow cytometer using CellQuest pro software (BD Biosciences, San Diego, CA, USA). Membrane fluorescence intensity was estimated and expressed as mean fluorescence intensity (MFI). Ismail AA, Donia HM, Ghatesh HM, Farid CI (2020) CD200/CD200 receptor axis in psoriasis vulgaris. PLoS ONE 15(3): e0230621. doi: 10.1371/journal.pone.0230621 Department of Dermatology, Venereology and Andrology; Faculty of Medicine; Alexandria University; Egypt. , Department of Dermatology, Venereology and Andrology; Faculty of Medicine; Alexandria University; Egypt. , Department of Clinical and Chemical pathology; Faculty of Medicine; Alexandria University; Alexandria; Egypt. , Department of Dermatology, Venereology and Andrology; Faculty of Medicine; Alexandria University; Alexandria; Egypt. https://doi.org/10.1371/journal.pone.0230621 1 2019 Carmen Farid, Aisha A. Mahmoud, Hanaa M. Donia, Hafsa M. Ghatesh 2019. CD200 and CD200R levels in psoriasis patients. protocols.io dx.doi.org/10.17504/protocols.io.6zihf4e 2021-03-29 03:10:07
Purification of avian egg yolk immunoglobulins using the water dilution method and precipitation with isopropanol.
 
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Angel Justiz-Vaillant 10.17504/protocols.io.bjvpkn5n University of the West Indies, [email protected] University of the West Indies St. Augustine 1 2020 Angel Justiz-Vaillant 2020. Purification of avian egg yolk immunoglobulins using the water dilution method and precipitation with isopropanol. . protocols.io dx.doi.org/10.17504/protocols.io.bjvpkn5n 2021-03-29 03:10:08
Remove unadsorbed phages
 
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Mathias Middelboe, Amy M. Chan, and Sif K. Bertelsen 10.17504/protocols.io.drb52m VERVE Net For use in "One-step growth experiments (cyanophages)" Manual of Aquatic Viral Ecology, Manual of Aquatic Viral Ecology, Manual of Aquatic Viral Ecology 1 2016 Mathias Middelboe, Amy M. Chan, and Sif K. Bertelsen 2016. Remove unadsorbed phages. protocols.io dx.doi.org/10.17504/protocols.io.drb52m 2021-03-29 03:10:08
Guide RNA Library Transduction of Cas9 Cancer Cell Lines
 
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Verity Goodwin, Emily Souster, Charlotte Beaver, Adam Jackson, Rizwan Ansari, Mathew Garnett, Fiona Behan 10.17504/protocols.io.bg2njyde Cellular Generation and Phenotyping This protocol is for the whole-genome CRISPR screening of stably expressing Cas9 cancer cell lines in triplicate using the commercially available Kusuke Yusa v1.1 whole genome gRNA library. It can be adapted for other gRNA libraries, under the assumption that there is a BFP reporter in the gRNA library. The protocol can be followed assuming the following is known:- The number of cells to be transduced - The number of days required for screening- How many cells to maintain throughout the screen- The number of cells required per pellet- The required coverage of the libraryThis protocol takes 16 days.Process diagram: Wellcome Sanger Institute, Wellcome Sanger Institute, Wellcome Sanger Institute, Wellcome Sanger Institute, Wellcome Sanger Institute, Wellcome Sanger Institute, Wellcome Sanger Institute 1 2020 Verity Goodwin, Emily Souster, Charlotte Beaver, Adam Jackson, Rizwan Ansari, Mathew Garnett, Fiona Behan 2020. Guide RNA Library Transduction of Cas9 Cancer Cell Lines. protocols.io dx.doi.org/10.17504/protocols.io.bg2njyde 2021-03-29 03:10:08
Hasselt University protocol for maternal hemodynamic profiling
 
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Wilfried Gyselaers, Kathleen Tomsin, Staelens Anneleen, Vonck Sharona 10.17504/protocols.io.tffejjn A maternal cardiovascular profile was assessed in every pregnant woman combining three non-invasive techniques to obtain information about arteries, veins, heart and body fluid content (Table 1). A standardized protocol was used as reported in previous studies, as enlisted below.  Hasselt University, Hasselt University, Hasselt University, Hasselt University 1 2018 Wilfried Gyselaers, Kathleen Tomsin, Staelens Anneleen, Vonck Sharona 2018. Hasselt University protocol for maternal hemodynamic profiling. protocols.io dx.doi.org/10.17504/protocols.io.tffejjn 2021-03-29 03:10:08
Scanorama
 
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Brian Hie, Bryan Bryson, Bonnie Berger 10.17504/protocols.io.9gch3sw Human Cell Atlas Method Development Community Scanorama enables efficient integration of heterogeneous scRNA-seq data sets. Massachusetts Institute of Technology, Massachusetts Institute of Technology, Massachusetts Institute of Technology http://scanorama.csail.mit.edu 1 2019 Brian Hie, Bryan Bryson, Bonnie Berger 2019. Scanorama. protocols.io dx.doi.org/10.17504/protocols.io.9gch3sw 2021-03-29 03:10:09
Genomic mapping of transformed DNA fragments
 
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Oskar Johansson, Adrian Clarke 10.17504/protocols.io.yq2fvye Protist Research to Optimize Tools in Genetics (PROT-G) Mapping of transformed DNA within mutated diatoms. A stepwise specific PCR reaction that allow for identification of genome location of transformed fragments such as antibiotic resistance genes or GFP constructs. Degenerate primer 1: 5' - GCCGGACTTAGCGAGCA TGTGTAGTACCTTGCCG ATCCTNASATANSATANT TC - 3'Degenerate primer 2: 5' - GCCGGACTTAGCGAGCA TGTGTAGTACCTTGCCG ATCCCNTSABGNACYTN CTG - 3'Degenerate primer 3: 5' - GCCGGACTTAGCGAGCA TGTGTAGTACCTTGCCG ATCCNGACGARWGANA WGAC - 3'Degenerate primer 4: 5' - GCCGGACTTAGCGAGCA TGTGTAGTACCTTGCCG ATCCTAHATGDAGKACN TAC - 3'Specific primer 1: 5' - GCCGGACTTAGCGAGCA TGTGTAG - 3'Specific primer 2: 5' - CATGTGTAGTACCTTGC CGATCC - 3'Internal primers must be designed with regard to each construct that are to be mapped. They should not overlap but instead cover consecutive stretches of known DNA sequence of the transformed construct. Biology and Environmental Sciences - Gothenburg University, Biology and Environmental Sciences - Gothenburg University 1 2019 Oskar Johansson, Adrian Clarke 2019. Genomic mapping of transformed DNA fragments. protocols.io dx.doi.org/10.17504/protocols.io.yq2fvye 2021-03-29 03:10:10
Measurement of heat production (thermogenesis) in cells using ERthermAC dye_microplate method
 
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Chih-Hao Wang, Yu-Hua Tseng, Yu-Hua Tseng 10.17504/protocols.io.bqprmvm6 Tseng Lab, Joslin Diabetes Center Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40402, Taiwan, Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40402, Taiwan, Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA, USA 1 2021 Chih-Hao Wang, Yu-Hua Tseng, Yu-Hua Tseng 2021. Measurement of heat production (thermogenesis) in cells using ERthermAC dye_microplate method. protocols.io dx.doi.org/10.17504/protocols.io.bqprmvm6 2021-03-29 03:10:07
High-performance fractional order terminal sliding mode control strategy for DC-DC Buck converter
 
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JL Wang, D Xu1, H Zhou, AN Bai, W Lu 10.17504/protocols.io.jtjcnkn EV , Teacher, cooperator, cooperator, cooperator 1 2018 JL Wang, D Xu1, H Zhou, AN Bai, W Lu 2018. High-performance fractional order terminal sliding mode control strategy for DC-DC Buck converter. protocols.io dx.doi.org/10.17504/protocols.io.jtjcnkn 2021-03-29 03:10:08
Measuring Wingstroke Amplitude with Strokelitude
 
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Björn Brembs 10.17504/protocols.io.c8mzu5 Brembs Lab Björn Brembs lab, Universität Regensburg http://lab.brembs.net/ 1 2015 Björn Brembs 2015. Measuring Wingstroke Amplitude with Strokelitude. protocols.io dx.doi.org/10.17504/protocols.io.c8mzu5 2021-03-29 03:10:08
RNA Isolation from Plant Tissue Protocol 10: TRIzol LS Reagent Method
 
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Eric Carpenter 10.17504/protocols.io.4rwgv7e GigaScience Press Implemented by: Michael Melkonian and Barbara Surek (algae) and Juan Carlos Villarreal (bryophytes)This protocol follows the procedures provided with the TRIzol LS Reagent (Invitrogen). TRIzol LS Reagent is a monophasic solution of phenol and guanidine isothiocyanate that can be used in isolation of total RNA from a wide variety of tissues and organisms, in addition to plants. This protocol was used in the isolation of total RNA from some algae samples (see Supplementary Table 1).This protocol is part of a collection of eighteen protocols used to isolate total RNA from plant tissue. (RNA Isolation from Plant Tissue Collection: https://www.protocols.io/view/rna-isolation-from-plant-tissue-439gyr6) 1 2019 Eric Carpenter 2019. RNA Isolation from Plant Tissue Protocol 10: TRIzol LS Reagent Method. protocols.io dx.doi.org/10.17504/protocols.io.4rwgv7e 2021-03-29 03:10:09
Alternative Splicing Analysis
 
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Hansheng Zhao 10.17504/protocols.io.phndj5e Zhao H, Gao Z, Wang L, Wang J, Wang S, Fei B, Chen C, Shi C, Liu X, Zhang H, Lou Y, Chen L, Sun H, Zhou X, Wang S, Zhang C, Xu H, Li L, Yang Y, Wei Y, Yang W, Gao Q, Yang H, Zhao S, Jiang Z, Chromosome-level reference genome and alternative splicing atlas of moso bamboo (). GigaScience 7(10). doi: 10.1093/gigascience/giy115 International Centre for Bamboo and Rattan https://doi.org/10.1093/gigascience/giy115 1 2018 Hansheng Zhao 2018. Alternative Splicing Analysis. protocols.io dx.doi.org/10.17504/protocols.io.phndj5e 2021-03-29 03:10:09

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