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Authors: Alyssa Alsante
Group: Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Van Etten, J. (n.d.). Formulation of Modified Bold's Basal Medium (MBBM). Retrieved from http://ncv.unl.edu/vanettenlab/
Proper citation: Alyssa Alsante 2016. MBBM Stock Solutions. protocols.io dx.doi.org/10.17504/protocols.io.geqbtdw Copy
Authors: Steven Wilhelm
Group: VERVE Net, The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols
Summary: Adapted from: Van Etten, J. (n.d.). Titering of Chlorella Viruses. Retrieved from http://ncv.unl.edu/vanettenlab/
Proper citation: Steven Wilhelm 2016. Virus Purification by Sucrose Density Gradients. protocols.io dx.doi.org/10.17504/protocols.io.grzbv76 Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from the original publication Stein, J (ED.) 1973. Handbook of Phycological methods. Culture methods and growth measurements. Cambridge University Press. 448 pp.
Proper citation: Dr. Steven Wilhelm 2017. Chu's-10 Media. protocols.io dx.doi.org/10.17504/protocols.io.h94b98w Copy
Authors: Dr. Steven Wilhelm
Group: VERVE Net, The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs
Summary: Please contact Dr. Steven Wilhelm ([email protected]) with any additional information regarding this protocol.
Proper citation: Dr. Steven Wilhelm 2019. Typing E. coli ATCC 11303 with fluorescently stained virus.. protocols.io dx.doi.org/10.17504/protocols.io.iivcce6 Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), jkuat
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from Csaky T. Z. (1948) On the estimation of bound hydroxylamine in biological materials. Acta Chem. Scand. 2, 250–454and A. H. Gillam, A. G. Lewis, and R. J. Andersen. Quantitative determination of hydroxamic acids Anal. Chem., 1981, 53 (6), pp 841–844 DOI: 10.1021/ac00229a023
Proper citation: Dr. Steven Wilhelm 2017. Czaky Test for Hydroxamate-Type Siderophores. protocols.io dx.doi.org/10.17504/protocols.io.icqcavw Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from: Watanabe, Makoto M. "Fresh-and salt-water forms of Spirulina platensis in axenic cultures." Bull. Jpn. Soc. Phycol. 25 (1977): 371-377.
Proper citation: Dr. Steven Wilhelm 2017. CT Media. protocols.io dx.doi.org/10.17504/protocols.io.iizccf6 Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from Ichimura, T. 1979 2. Isolation and culture methods of algae. 2.5.B. Freshwater algae [2. Sôrui no bunri to baiyôhô. 2.5.B. Tansui sôrui]. In Methods in Phycological Studies [Sôrui Kenkyûhô], Eds. by Nishizawa, K. & Chihara, M., Kyoritsu Shuppan, Tokyo, p. 294-305 ( in Japanese without English title ).
Proper citation: Dr. Steven Wilhelm 2017. MA Media. protocols.io dx.doi.org/10.17504/protocols.io.ibhcaj6 Copy
Authors: Dr. Steven Wilhelm, Samantha Coy
Group: Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols
Summary: Van Etten, J. (n.d.). Formulation of Modified Bold's Basal Medium (MBBM). Retrieved from http://ncv.unl.edu/vanettenlab/ Contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol.
Proper citation: Dr. Steven Wilhelm, Samantha Coy 2017. MBBM Media. protocols.io dx.doi.org/10.17504/protocols.io.hg3b3yn Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from Schwyn, B. & Neilands, J. B. Universal chemical assay for the detection and determination of siderophores.Anal Biochem, 160:147-156 (1987).
Proper citation: Dr. Steven Wilhelm 2017. Chrome Azurol S (CAS) Liquid Assay for Iron-Binding Compounds. protocols.io dx.doi.org/10.17504/protocols.io.ifwcbpe Copy
Authors: Steven Wilhelm, Gary Lecleir
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Proper citation: Steven Wilhelm, Gary Lecleir 2019. Emergency Contacts. protocols.io dx.doi.org/10.17504/protocols.io.6c6haze Copy
Authors: Steven Wilhelm
Group: VERVE Net, The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols
Summary: Adapted from: Van Etten, J. (n.d.). Titering of Chlorella Viruses. Retrieved from http://ncv.unl.edu/vanettenlab/
Proper citation: Steven Wilhelm 2017. Virus Purification by Sucrose Density Gradients. protocols.io dx.doi.org/10.17504/protocols.io.g4gbytw Copy
Authors: Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.This cleaning protocol is meant to be used when there is an excess of particulate buildup in the FCM. Blockages can be detected by the failure of the FCM to successfully plot data in the scatter plot even though the machine is in the ‘acquire sample’ step. This will be visualized on the scatter plot as either extreme alterations to the forward and side scatter plot (a Etch A Sketch like drawing) or a complete absence of cell populations that should be there otherwise. If blockages are not addressed immediately, counts will be significantly affected because the machine cannot tell when there is a blockage- it will think volume is passing through the flow cell when it isn’t, which will artificially lower your cell counts.
Proper citation: Steven Wilhelm 2017. Guava Flow Cytometry Cleaning. protocols.io dx.doi.org/10.17504/protocols.io.iwbcfan Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from the original publication Morel, F. M. M., J. C. Westall, J. G. Reuter & J. P. Chaplick, 1975. Description of the algal growth media ‘Aquil’ and ‘Fraquil’. Water Quality Laboratory, Ralph Parsons Laboratory for Water Resources and Hydrodynamics, Massachusetts Institute of Technology, Technical Report 16, 33 pp.
Proper citation: Dr. Steven Wilhelm 2017. Fraquil Media. protocols.io dx.doi.org/10.17504/protocols.io.h93b98n Copy
Authors: Steven Wilhelm, Gary Lecleir, Ashley Humphrey
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: BIOLOGICAL SPILL RESPONSE method to be used in the event of a biosafety incident.
Proper citation: Steven Wilhelm, Gary Lecleir, Ashley Humphrey 2019. Cleaning up a biohazardous spill in a biosafety cabinet. protocols.io dx.doi.org/10.17504/protocols.io.wghfbt6 Copy
Authors: Dr. Steven Wilhelm, Samantha Coy
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol.
Proper citation: Dr. Steven Wilhelm, Samantha Coy 2017. Long term Cryopreservation of Chloroviruses by Infection of Chlorella. protocols.io dx.doi.org/10.17504/protocols.io.gwhbxb6 Copy
Authors: Steven Wilhelm, Gary Lecleir
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: This document serves as a reference for chemicals used and stored in room 624 in the Science and Engineering Building at The University of Tennessee. For more information, contact Steven Wilhelm or Gary LeCleir.
Proper citation: Steven Wilhelm, Gary Lecleir 2019. Chemical Inventory for Wilhelm Lab 624. protocols.io dx.doi.org/10.17504/protocols.io.6cwhaxe Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.
Proper citation: Dr. Steven Wilhelm 2017. M12 Tangential Flow Filtration . protocols.io dx.doi.org/10.17504/protocols.io.icscawe Copy
Authors: Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Proper citation: Steven Wilhelm 2017. Screening Sequencing Datasets for Marker Genes in CLC Genomics. protocols.io dx.doi.org/10.17504/protocols.io.g5vby66 Copy
Authors: Dr. Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs
Summary: Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.
Proper citation: Dr. Steven Wilhelm 2017. Chu's-10 Metal Solution. protocols.io dx.doi.org/10.17504/protocols.io.h95b986 Copy
Authors: Gary Lecleir, Steven Wilhelm
Group: The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs)
Summary: Welcome to the Wilhelm Lab. This short training is to help orient those that are learning how to work in a laboratory environment as well as help those that are joining our lab to become acquainted with our procedures.
Proper citation: Gary Lecleir, Steven Wilhelm 2019. UTK Laboratory Orientation/Training. protocols.io dx.doi.org/10.17504/protocols.io.6c3hayn Copy
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