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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
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MBBM Stock Solutions Resource Report Resource Website |
Alyssa Alsante | 10.17504/protocols.io.geqbtdw | Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Van Etten, J. (n.d.). Formulation of Modified Bold's Basal Medium (MBBM). Retrieved from http://ncv.unl.edu/vanettenlab/ | http://ncv.unl.edu/vanettenlab/ | 1 | 2016 | Alyssa Alsante 2016. MBBM Stock Solutions. protocols.io dx.doi.org/10.17504/protocols.io.geqbtdw | 2021-03-29 03:12:07 | |||
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Virus Purification by Sucrose Density Gradients Resource Report Resource Website |
Steven Wilhelm | 10.17504/protocols.io.grzbv76 | VERVE Net, The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols | Adapted from: Van Etten, J. (n.d.). Titering of Chlorella Viruses. Retrieved from http://ncv.unl.edu/vanettenlab/ | The University of Tennessee, Knoxville | http://ncv.unl.edu/vanettenlab/Chlorovirus%20Purification.pdf | 1 | 2016 | Steven Wilhelm 2016. Virus Purification by Sucrose Density Gradients. protocols.io dx.doi.org/10.17504/protocols.io.grzbv76 | 2021-03-29 03:12:07 | ||
|
Chu's-10 Media Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.h94b98w | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from the original publication Stein, J (ED.) 1973. Handbook of Phycological methods. Culture methods and growth measurements. Cambridge University Press. 448 pp. | 3 | 2017 | Dr. Steven Wilhelm 2017. Chu's-10 Media. protocols.io dx.doi.org/10.17504/protocols.io.h94b98w | 2021-03-29 03:12:12 | ||||
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Typing E. coli ATCC 11303 with fluorescently stained virus. Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.iivcce6 | VERVE Net, The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs | Please contact Dr. Steven Wilhelm ([email protected]) with any additional information regarding this protocol. | Microbiology Department. University of Tennessee, Knoxville. | 1 | 2019 | Dr. Steven Wilhelm 2019. Typing E. coli ATCC 11303 with fluorescently stained virus.. protocols.io dx.doi.org/10.17504/protocols.io.iivcce6 | 2021-03-29 03:12:14 | |||
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Czaky Test for Hydroxamate-Type Siderophores Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.icqcavw | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), jkuat | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from Csaky T. Z. (1948) On the estimation of bound hydroxylamine in biological materials. Acta Chem. Scand. 2, 250–454and A. H. Gillam, A. G. Lewis, and R. J. Andersen. Quantitative determination of hydroxamic acids Anal. Chem., 1981, 53 (6), pp 841–844 DOI: 10.1021/ac00229a023 | 1 | 2017 | Dr. Steven Wilhelm 2017. Czaky Test for Hydroxamate-Type Siderophores. protocols.io dx.doi.org/10.17504/protocols.io.icqcavw | 2021-03-29 03:11:57 | ||||
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CT Media Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.iizccf6 | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from: Watanabe, Makoto M. "Fresh-and salt-water forms of Spirulina platensis in axenic cultures." Bull. Jpn. Soc. Phycol. 25 (1977): 371-377. | 2 | 2017 | Dr. Steven Wilhelm 2017. CT Media. protocols.io dx.doi.org/10.17504/protocols.io.iizccf6 | 2021-03-29 03:11:58 | ||||
|
MA Media Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.ibhcaj6 | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from Ichimura, T. 1979 2. Isolation and culture methods of algae. 2.5.B. Freshwater algae [2. Sôrui no bunri to baiyôhô. 2.5.B. Tansui sôrui]. In Methods in Phycological Studies [Sôrui Kenkyûhô], Eds. by Nishizawa, K. & Chihara, M., Kyoritsu Shuppan, Tokyo, p. 294-305 ( in Japanese without English title ). | 2 | 2017 | Dr. Steven Wilhelm 2017. MA Media. protocols.io dx.doi.org/10.17504/protocols.io.ibhcaj6 | 2021-03-29 03:11:59 | ||||
|
MBBM Media Resource Report Resource Website |
Dr. Steven Wilhelm, Samantha Coy | 10.17504/protocols.io.hg3b3yn | Protist Research to Optimize Tools in Genetics (PROT-G), The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols | Van Etten, J. (n.d.). Formulation of Modified Bold's Basal Medium (MBBM). Retrieved from http://ncv.unl.edu/vanettenlab/ Contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol. | , | http://ncv.unl.edu/vanettenlab/MBBM.pdf | 3 | 2017 | Dr. Steven Wilhelm, Samantha Coy 2017. MBBM Media. protocols.io dx.doi.org/10.17504/protocols.io.hg3b3yn | 2021-03-29 03:12:02 | ||
|
Chrome Azurol S (CAS) Liquid Assay for Iron-Binding Compounds Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.ifwcbpe | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Modified from Schwyn, B. & Neilands, J. B. Universal chemical assay for the detection and determination of siderophores.Anal Biochem, 160:147-156 (1987). | 2 | 2017 | Dr. Steven Wilhelm 2017. Chrome Azurol S (CAS) Liquid Assay for Iron-Binding Compounds. protocols.io dx.doi.org/10.17504/protocols.io.ifwcbpe | 2021-03-29 03:12:02 | ||||
|
Emergency Contacts Resource Report Resource Website |
Steven Wilhelm, Gary Lecleir | 10.17504/protocols.io.6c6haze | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | The University of Tennessee, Knoxville, The University of Tennessee, Knoxville | 1 | 2019 | Steven Wilhelm, Gary Lecleir 2019. Emergency Contacts. protocols.io dx.doi.org/10.17504/protocols.io.6c6haze | 2021-03-29 03:08:20 | ||||
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Virus Purification by Sucrose Density Gradients Resource Report Resource Website |
Steven Wilhelm | 10.17504/protocols.io.g4gbytw | VERVE Net, The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols | Adapted from: Van Etten, J. (n.d.). Titering of Chlorella Viruses. Retrieved from http://ncv.unl.edu/vanettenlab/ | The University of Tennessee, Knoxville | http://ncv.unl.edu/vanettenlab/Chlorovirus%20Purification.pdf | 2 | 2017 | Steven Wilhelm 2017. Virus Purification by Sucrose Density Gradients. protocols.io dx.doi.org/10.17504/protocols.io.g4gbytw | 2021-03-29 03:09:31 | ||
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Guava Flow Cytometry Cleaning Resource Report Resource Website |
Steven Wilhelm | 10.17504/protocols.io.iwbcfan | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), Samantha R Coy's Protocols | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.This cleaning protocol is meant to be used when there is an excess of particulate buildup in the FCM. Blockages can be detected by the failure of the FCM to successfully plot data in the scatter plot even though the machine is in the ‘acquire sample’ step. This will be visualized on the scatter plot as either extreme alterations to the forward and side scatter plot (a Etch A Sketch like drawing) or a complete absence of cell populations that should be there otherwise. If blockages are not addressed immediately, counts will be significantly affected because the machine cannot tell when there is a blockage- it will think volume is passing through the flow cell when it isn’t, which will artificially lower your cell counts. | 1 | 2017 | Steven Wilhelm 2017. Guava Flow Cytometry Cleaning. protocols.io dx.doi.org/10.17504/protocols.io.iwbcfan | 2021-03-29 03:09:34 | ||||
|
Fraquil Media Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.h93b98n | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol.Adapted from the original publication Morel, F. M. M., J. C. Westall, J. G. Reuter & J. P. Chaplick, 1975. Description of the algal growth media ‘Aquil’ and ‘Fraquil’. Water Quality Laboratory, Ralph Parsons Laboratory for Water Resources and Hydrodynamics, Massachusetts Institute of Technology, Technical Report 16, 33 pp. | 3 | 2017 | Dr. Steven Wilhelm 2017. Fraquil Media. protocols.io dx.doi.org/10.17504/protocols.io.h93b98n | 2021-03-29 03:09:38 | ||||
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Cleaning up a biohazardous spill in a biosafety cabinet Resource Report Resource Website |
Steven Wilhelm, Gary Lecleir, Ashley Humphrey | 10.17504/protocols.io.wghfbt6 | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | BIOLOGICAL SPILL RESPONSE method to be used in the event of a biosafety incident. | The University of Tennessee, Knoxville, The University of Tennessee, Knoxville, University of Tennessee, Knoxville | 1 | 2019 | Steven Wilhelm, Gary Lecleir, Ashley Humphrey 2019. Cleaning up a biohazardous spill in a biosafety cabinet. protocols.io dx.doi.org/10.17504/protocols.io.wghfbt6 | 2021-03-29 03:09:45 | |||
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Long term Cryopreservation of Chloroviruses by Infection of Chlorella Resource Report Resource Website |
Dr. Steven Wilhelm, Samantha Coy | 10.17504/protocols.io.gwhbxb6 | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Contact Dr. Steven Wilhelm ([email protected]) or Samantha Coy ([email protected]) for additional information regarding this protocol. | , | 1 | 2017 | Dr. Steven Wilhelm, Samantha Coy 2017. Long term Cryopreservation of Chloroviruses by Infection of Chlorella. protocols.io dx.doi.org/10.17504/protocols.io.gwhbxb6 | 2021-03-29 03:09:51 | |||
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Chemical Inventory for Wilhelm Lab 624 Resource Report Resource Website |
Steven Wilhelm, Gary Lecleir | 10.17504/protocols.io.6cwhaxe | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | This document serves as a reference for chemicals used and stored in room 624 in the Science and Engineering Building at The University of Tennessee. For more information, contact Steven Wilhelm or Gary LeCleir. | The University of Tennessee, Knoxville, The University of Tennessee, Knoxville | 1 | 2019 | Steven Wilhelm, Gary Lecleir 2019. Chemical Inventory for Wilhelm Lab 624. protocols.io dx.doi.org/10.17504/protocols.io.6cwhaxe | 2021-03-29 03:09:52 | |||
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M12 Tangential Flow Filtration Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.icscawe | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol. | 1 | 2017 | Dr. Steven Wilhelm 2017. M12 Tangential Flow Filtration . protocols.io dx.doi.org/10.17504/protocols.io.icscawe | 2021-03-29 03:08:35 | ||||
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Screening Sequencing Datasets for Marker Genes in CLC Genomics Resource Report Resource Website |
Steven Wilhelm | 10.17504/protocols.io.g5vby66 | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | The University of Tennessee, Knoxville | 1 | 2017 | Steven Wilhelm 2017. Screening Sequencing Datasets for Marker Genes in CLC Genomics. protocols.io dx.doi.org/10.17504/protocols.io.g5vby66 | 2021-03-29 03:08:33 | ||||
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Chu's-10 Metal Solution Resource Report Resource Website |
Dr. Steven Wilhelm | 10.17504/protocols.io.h95b986 | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs), CyanoHABs | Please contact Dr. Steven Wilhelm ([email protected]) for additional information regarding this protocol. | 2 | 2017 | Dr. Steven Wilhelm 2017. Chu's-10 Metal Solution. protocols.io dx.doi.org/10.17504/protocols.io.h95b986 | 2021-03-29 03:08:38 | ||||
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UTK Laboratory Orientation/Training Resource Report Resource Website |
Gary Lecleir, Steven Wilhelm | 10.17504/protocols.io.6c3hayn | The Aquatic Microbial Ecology Research Group - AMERG (The Buchan, Zinser and Wilhelm labs) | Welcome to the Wilhelm Lab. This short training is to help orient those that are learning how to work in a laboratory environment as well as help those that are joining our lab to become acquainted with our procedures. | University of Illinois at Urbana-Champaign, The University of Tennessee, Knoxville | 1 | 2019 | Gary Lecleir, Steven Wilhelm 2019. UTK Laboratory Orientation/Training. protocols.io dx.doi.org/10.17504/protocols.io.6c3hayn | 2021-03-29 03:08:37 |
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