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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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BioBrick E0546 Upstream Reaction Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cpivkd | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/1/01/01/digestion-protocol-e0546 | 1 | 2015 | New England Biolabs 2015. BioBrick E0546 Upstream Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cpivkd | 2021-03-29 03:08:25 | |||
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T4 PNK Reaction Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cpevjd | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2012/08/24/radioactive-labeling-with-t4-pnk-or-t4-pnk-3-phosphatase-minus | 1 | 2015 | New England Biolabs 2015. T4 PNK Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cpevjd | 2021-03-29 03:08:24 | |||
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NEBNext Ultra II FS DNA Module E7810 Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.n34dgqw | New England Biolabs (NEB) | The NEBNext Ultra II FS DNA Module contains the enzymes and buffers required to convert a broad range of input amounts of intact DNA into fragmented DNA with 5´ phosphorylated 3´ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595) and with the NEBNext Ultra II Q5 Master Mix (NEB #M0544) if amplification is required. The fast, user-friendly workflow has minimal hands on time.Note: The Ultra II FS Module is not compatible with bisulfite conversion workflows | New England Biolabs | 1 | 2018 | New England Biolabs 2018. NEBNext Ultra II FS DNA Module E7810. protocols.io dx.doi.org/10.17504/protocols.io.n34dgqw | 2021-03-29 03:08:29 | |||
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Mixture for M0491 Q5 PCR Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cieubd | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2013/12/13/pcr-using-q5-high-fidelity-dna-polymerase-m0491 | 1 | 2015 | New England Biolabs 2015. Mixture for M0491 Q5 PCR. protocols.io dx.doi.org/10.17504/protocols.io.cieubd | 2021-03-29 03:11:20 | |||
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Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.wkdfcs6 | New England Biolabs (NEB) | The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) depletes both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochodrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g. FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications. | New England Biolabs | 2 | 2018 | New England Biolabs 2018. Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, E7765). protocols.io dx.doi.org/10.17504/protocols.io.wkdfcs6 | 2021-03-29 03:11:18 | |||
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Electroporation of Cas9 RNP (ribonucleoprotein) into adherent cells using the Neon® Electroporation Resource Report Resource Website |
New England Biolabs, Inc. | 10.17504/protocols.io.frkbm4w | New England Biolabs (NEB) | Cas9 nuclease may be used in vivo to create targeted genome modifications. There are several ways in which to introduce Cas9-guide RNA complexes into cells. Here we present a method for the introduction of Cas9 RNP’s into HEK293 FT cells using the Thermo Fisher Neon Electroporation System. This method uses a final concentration of 20 nM RNP per transfection in a 24-well culture plate. | , | https://www.neb.com/protocols/2016/07/26/electroporation-of-cas9-rnp-ribonucleoprotein-into-adherent-cells-using-the-neon-electroporation | 1 | 2016 | New England Biolabs, Inc. 2016. Electroporation of Cas9 RNP (ribonucleoprotein) into adherent cells using the Neon® Electroporation. protocols.io dx.doi.org/10.17504/protocols.io.frkbm4w | 2021-03-29 03:11:29 | ||
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T4 PNK Minus Reaction Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cp3vqm | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/1/01/01/non-radioactive-phosphorylation-with-t4-pnk-or-pnk3-phosphatase-minus | 1 | 2015 | New England Biolabs 2015. T4 PNK Minus Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cp3vqm | 2021-03-29 03:11:43 | |||
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E2621 DNA Assembly Reaction Resource Report Resource Website |
NEB | 10.17504/protocols.io.cwcxav | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2014/11/26/nebuilder-hifi-dna-assembly-reaction-protocol | 1 | 2015 | NEB 2015. E2621 DNA Assembly Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cwcxav | 2021-03-29 03:11:41 | |||
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Quick Protocol for Monarch® PCR & DNA Cleanup Kit (5 ?g) (NEB #T1030) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.bp9emr3e | New England Biolabs (NEB) | This is the "quick" version of Monarch® PCR & DNA Cleanup Kit (5 μg) Protocol (NEB #T1030). For the full protocol, please click here. | New England Biolabs | https://www.neb.com/protocols/2015/12/08/quick-protocol-for-monarch-pcr-dna-cleanup-kit-5-g-t1030 | 4 | 2020 | New England Biolabs 2020. Quick Protocol for Monarch® PCR & DNA Cleanup Kit (5 ?g) (NEB #T1030). protocols.io dx.doi.org/10.17504/protocols.io.bp9emr3e | 2021-03-29 03:11:47 | ||
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Protocol for use with NEBNext® Small RNA Library Prep Set for Illumina® (E7300, E7580, E7560, E7330) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.t7qermw | New England Biolabs (NEB) | The NEBNext Multiplex Small RNA Library Prep Set for Illumina contains the adaptors, primers, enzymes and buffers required to convert small RNAs into indexed libraries for next generation sequencing on the Illumina platform. The novel workflow has been optimized to minimized adaptor dimers, while producing high-yield, high-diversity libraries. The unique workflow of the NEBNext® Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol. Minimized adaptor-dimer contaminationHigh yieldsInput RNA can be Total RNASuitable for methylated small RNAs (e.g., piRNAs) as well as unmethylated small RNAsMultiplex Small RNA Library Prep Workflow This kit includes a novel protocol that results in higher yields and lower adaptor-dimer contamination. | New England Biolabs | https://www.neb.com/products/e7300-nebnext-multiplex-small-rna-library-prep-set-for-illumina-set-1#Protocols%20&%20Manuals_Protocols | 1 | 2018 | New England Biolabs 2018. Protocol for use with NEBNext® Small RNA Library Prep Set for Illumina® (E7300, E7580, E7560, E7330). protocols.io dx.doi.org/10.17504/protocols.io.t7qermw | 2021-03-29 03:11:51 | ||
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Cas9 M0386 Mixture Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.ch3t8m | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/2014/05/01/in-vitro-digestion-of-dna-with-cas9-nuclease-s-pyogenes-m0386 | 1 | 2014 | New England Biolabs 2014. Cas9 M0386 Mixture. protocols.io dx.doi.org/10.17504/protocols.io.ch3t8m | 2021-03-29 03:13:22 | |||
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PCR Using Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.7sehnbe | New England Biolabs (NEB) | Please note that protocols with Q5U Hot Start High-Fidelity DNA Polymerase may differ from protocols with other polymerases. Conditions recommended in the protocols should be used for optimal performance. | New England Biolabs | https://www.neb.com/protocols/2019/07/02/pcr-using-q5u-hot-start-high-fidelity-dna-polymerase-neb-m0515 | 1 | 2020 | New England Biolabs 2020. PCR Using Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515). protocols.io dx.doi.org/10.17504/protocols.io.7sehnbe | 2021-03-29 03:13:24 | ||
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High Efficiency Transformation Protocol (C2987H) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.chgt3v | New England Biolabs (NEB) | This is the correct protocol if you are using the C2987H cells. If you are using the C2987I cells, please refer to this protocol. | New England Biolabs | https://www.neb.com/protocols/1/01/01/high-efficiency-transformation-protocol-c2987 | 1 | 2014 | New England Biolabs 2014. High Efficiency Transformation Protocol (C2987H). protocols.io dx.doi.org/10.17504/protocols.io.chgt3v | 2021-03-29 03:13:45 | ||
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Cleavage of the Fusion Protein (TEV Protease) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.bfd9ji96 | New England Biolabs (NEB) | TEV Protease, also known as Tobacco Etch Virus (TEV) Protease, is a highly specific cysteine protease that recognizes the amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) and cleaves between the Gln and Gly/Ser residues. It is often used for the removal of affinity purification tags such as maltose-binding protein (MBP) or poly-histidine from fusion proteins. TEV Protease has a 7xHis-tag for easy removal from a reaction using nickel affinity resins and has been engineered to improve thermal stability and decrease autolysis. | New England Biolabs | 1 | 2020 | New England Biolabs 2020. Cleavage of the Fusion Protein (TEV Protease). protocols.io dx.doi.org/10.17504/protocols.io.bfd9ji96 | 2021-03-29 03:13:41 | |||
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Making your own electrocompetent cells Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.crtv6m | New England Biolabs (NEB) | This is the protocol for making two 250 ml cultures of electrocompetent cells | New England Biolabs | https://www.neb.com/protocols/2012/06/21/making-your-own-electrocompetent-cells | 1 | 2015 | New England Biolabs 2015. Making your own electrocompetent cells. protocols.io dx.doi.org/10.17504/protocols.io.crtv6m | 2021-03-29 03:13:50 | ||
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Multi-temperature Double Digest (single buffer) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cjkukv | New England Biolabs (NEB) | This is the Double Digest Protocol with Standard Restriction Enzymes, in the same buffer, using two separate incubation temperatures for the enzymes. | New England Biolabs | https://www.neb.com/protocols/2014/05/07/double-digest-protocol-with-standard-restriction-enzymes | 1 | 2014 | New England Biolabs 2014. Multi-temperature Double Digest (single buffer). protocols.io dx.doi.org/10.17504/protocols.io.cjkukv | 2021-03-29 03:12:36 | ||
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NEBExpress Ni Resin Pressurized Column Typical Protocol (NEB #S1428) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.bfa4jigw | New England Biolabs (NEB) | NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface. It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and β-Mercaptoethanol.Purification of ≥10 mg His-tagged protein per 1 ml of resinIntended for use in gravity or pressure flow columns, and batch purificationsHigh specific binding of His-tagged proteins yielding purities of >95%Strong nickel ion binding provides excellent resistance to EDTA and reducing agents. Compatible with commercially available detergent-based cell lysis reagentsIsolation and purification of His-tagged fusion proteins under native or denaturing conditions | New England Biolabs | https://www.neb.com/protocols/2019/09/10/nebexpress-ni-resin-pressurized-column-protocol-neb-s1428 | 1 | 2020 | New England Biolabs 2020. NEBExpress Ni Resin Pressurized Column Typical Protocol (NEB #S1428). protocols.io dx.doi.org/10.17504/protocols.io.bfa4jigw | 2021-03-29 03:12:42 | ||
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BioBrick E0546 Downstream Reaction Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cpkvkv | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/1/01/01/digestion-protocol-e0546 | 1 | 2015 | New England Biolabs 2015. BioBrick E0546 Downstream Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cpkvkv | 2021-03-29 03:12:40 | |||
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NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®) E7760 Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.bsbrnam6 | New England Biolabs (NEB), Coronavirus Method Development Community | This protocol details methods for the NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®), NEB #E7660S/L 24/96 reactions. | New England Biolabs | https://www.neb.com/-/media/nebus/files/manuals/manuale7660a.pdf?rev=4a88553b27064ccdbcdce76d05a2627c&hash=09A89395846012AA8A389C1DCFCE92F8 | 1 | 2021 | New England Biolabs 2021. NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®) E7760. protocols.io dx.doi.org/10.17504/protocols.io.bsbrnam6 | 2021-03-29 03:12:53 | ||
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High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019H) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.nkwdcxe | New England Biolabs (NEB) | This is the correct protocol if you are using the C3019H cells. If you are using the C3019I cells, please refer to this protocol. | NEB, 240 County RoadIpswich, MA 01938-2723 | https://www.neb.com/protocols/1/01/01/high-efficiency-transformation-protocol-c3019 | 4 | 2018 | New England Biolabs 2018. High Efficiency Transformation Protocol using NEB 10-beta Competent E. coli (C3019H). protocols.io dx.doi.org/10.17504/protocols.io.nkwdcxe | 2021-03-29 03:14:27 |
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