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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
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Isolation of Nuelci from Adult_Human_Brain_Tissue Resource Report Resource Website |
LeinU01 BRAIN Grant | 10.17504/protocols.io.y6pfzdn | BICCN, Allen Institute for Brain Science | Isolation of nuclei from frozen adult human brain tissue or thawed and microdissected brain tissue sections for FPCR and/or RNA-seq analysis. | Allen Institute | 1 | 2019 | LeinU01 BRAIN Grant 2019. Isolation of Nuelci from Adult_Human_Brain_Tissue. protocols.io dx.doi.org/10.17504/protocols.io.y6pfzdn | 2021-03-29 03:07:57 | |||
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Intramuscular Injection Adult Mouse Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bfzfjp3n | BICCN, Allen Institute for Brain Science | This protocol describes the general procedures used for intramuscular injection in adult mice. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. Intramuscular Injection Adult Mouse. protocols.io dx.doi.org/10.17504/protocols.io.bfzfjp3n | 2021-03-29 03:08:07 | |||
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Zeiss AxioImager Multi Channel 20X Image Capture Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bdpki5kw | BICCN, Allen Institute for Brain Science | This protocol describes the capture of 20X multi-channel images using human and mouse tissue.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. Zeiss AxioImager Multi Channel 20X Image Capture. protocols.io dx.doi.org/10.17504/protocols.io.bdpki5kw | 2021-03-29 03:08:14 | |||
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Leica SP8 Confocal Imaging Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bd8wi9xe | BICCN, Allen Institute for Brain Science | This protocol describes the basic setup and scanning using the Leica SP8 confocal microscope of fluorescently-labeled mouse brain tissue sections mounted on positively charged 1” x 3” microscope slides.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. Leica SP8 Confocal Imaging. protocols.io dx.doi.org/10.17504/protocols.io.bd8wi9xe | 2021-03-29 03:08:15 | |||
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Post Patch Clamp Slice Fixation Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bctciwiw | BICCN, Allen Institute for Brain Science | This protocol describes the process to fix recorded/filled cells from mouse and human brain slices with 4% PFA/2.5% Glutaraldehyde in PBS for future histochemical processing. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. Post Patch Clamp Slice Fixation. protocols.io dx.doi.org/10.17504/protocols.io.bctciwiw | 2021-03-29 03:08:17 | |||
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Human Neurosurgical Tissue Processing Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bctdiwi6 | BICCN, Allen Institute for Brain Science | This protocol describes the procedure for receiving human surgical tissue, followed by trimming, mounting, embedding, and slicing under sterile conditions in order to produce tissue suitable for both slice culture as well as acute slice electrophysiology recording. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. Human Neurosurgical Tissue Processing. protocols.io dx.doi.org/10.17504/protocols.io.bctdiwi6 | 2021-03-29 03:08:19 | |||
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TissueCyte Specimen Embedding Acrylamide Coembedding Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.ber4jd8w | BICCN, Allen Institute for Brain Science | This protocol describes the preparation of a fixed mouse brain specimen bonded to an agarose block for blockface imaging and sectioning using the TissueCyte 1000 system.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 3 | 2020 | Allen Institute for Brain Science 2020. TissueCyte Specimen Embedding Acrylamide Coembedding. protocols.io dx.doi.org/10.17504/protocols.io.ber4jd8w | 2021-03-29 03:07:36 | |||
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10Xv2 RNASeq Sample Processing Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bq68mzhw | BICCN, Allen Institute for Brain Science | Allows for rapid generation of 3’ transcriptomic-NGS-ready- single-cell-libraries from pools of cells.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 1 | 2021 | Allen Institute for Brain Science 2021. 10Xv2 RNASeq Sample Processing. protocols.io dx.doi.org/10.17504/protocols.io.bq68mzhw | 2021-03-29 03:07:29 | |||
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Patch-Seq Recording and Extraction Detailed Protocol Resource Report Resource Website |
Brian Lee, Kristen Hadley, Allen Institute for Brain Science | 10.17504/protocols.io.bpbuminw | Allen Institute for Brain Science | This protocol is a detailed version of the Allen Institute's Patch-Seq Recording and Extraction protocol, which describes the process to obtain electrophysiological recordings and cellular contents from neurons in postnatal mouse and/or human brain slices.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute, Allen Institute, Allen Institute | 1 | 2020 | Brian Lee, Kristen Hadley, Allen Institute for Brain Science 2020. Patch-Seq Recording and Extraction Detailed Protocol. protocols.io dx.doi.org/10.17504/protocols.io.bpbuminw | 2021-03-29 03:07:37 | |||
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Artificial Cerebrospinal Fluid VIII (ACSF.VIII) Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bdpai5ie | BICCN, Allen Institute for Brain Science | This protocol describes how to make Artificial Cerebrospinal Fluid VIII (ACSF.VIII). ACSF.VIII is used for multiple applications including incubation of fresh human brain slices prior to electrophysiological recording.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. Artificial Cerebrospinal Fluid VIII (ACSF.VIII). protocols.io dx.doi.org/10.17504/protocols.io.bdpai5ie | 2021-03-29 03:07:41 | |||
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Coating Slides with Gelatin Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bf64jrgw | BICCN, Allen Institute for Brain Science | The protocol describes how 1x3 glass microscope slides are coated with gelatin to improve adherence of fixed adult mouse brain sections or other tissue to slides.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 2 | 2020 | Allen Institute for Brain Science 2020. Coating Slides with Gelatin. protocols.io dx.doi.org/10.17504/protocols.io.bf64jrgw | 2021-03-29 03:11:08 | |||
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Pulling Pipettes using the DMZ-Zeitz Universal Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bepzjdp6 | BICCN, Allen Institute for Brain Science | Protocol is used for the manufacture of glass pipettes for patch clamp electrophysiology using the DMZ-Zeitz Universal puller.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 3 | 2020 | Allen Institute for Brain Science 2020. Pulling Pipettes using the DMZ-Zeitz Universal. protocols.io dx.doi.org/10.17504/protocols.io.bepzjdp6 | 2021-03-29 03:11:22 | |||
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SMARTerV4 (0.5x) Amplification for single-cell or single-nuclei RNASeq Protocol Resource Report Resource Website |
ZengU19 BRAIN grant | 10.17504/protocols.io.7d5hi86 | BICCN, Allen Institute for Brain Science | Protocol to generate full-length cDNA from single cells, or nuclei, using Takara SMARTer V4. | Allen Institute | 1 | 2019 | ZengU19 BRAIN grant 2019. SMARTerV4 (0.5x) Amplification for single-cell or single-nuclei RNASeq Protocol. protocols.io dx.doi.org/10.17504/protocols.io.7d5hi86 | 2021-03-29 03:11:50 | |||
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FACS Single Cell Sorting Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bambic2n | BICCN, Allen Institute for Brain Science | This protocol provides step by step instructions for performing single-cell sorting by using BD FACSAria II machine or BD FACSAria Fusion machine and BD FACSDiva software. | Allen Institute | 1 | 2019 | Allen Institute for Brain Science 2019. FACS Single Cell Sorting. protocols.io dx.doi.org/10.17504/protocols.io.bambic2n | 2021-03-29 03:13:28 | |||
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Maintenance of TissueCyte System Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.ber3jd8n | BICCN, Allen Institute for Brain Science | This protocols provides detailed instructions for procedures needed for maintenance of the TissueCyte scanning systems.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 3 | 2020 | Allen Institute for Brain Science 2020. Maintenance of TissueCyte System. protocols.io dx.doi.org/10.17504/protocols.io.ber3jd8n | 2021-03-29 03:13:33 | |||
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Isolation of Nuclei from Adult_Human_Brain_Tissue Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bq7dmzi6 | BICCN, Allen Institute for Brain Science | Isolation of nuclei from frozen adult human brain tissue or thawed and microdissected brain tissue sections for FPCR and/or RNA-seq analysis. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U01MH114812. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 1 | 2021 | Allen Institute for Brain Science 2021. Isolation of Nuclei from Adult_Human_Brain_Tissue. protocols.io dx.doi.org/10.17504/protocols.io.bq7dmzi6 | 2021-03-29 03:13:35 | |||
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20% Paraformaldehyde in 5X PBS Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bg4zjyx6 | BICCN, Allen Institute for Brain Science | This protocol is used to prepare 20% Paraformaldehyde (PFA) in 5X Phosphate-buffered saline (PBS). 20% PFA in 5X PBS is utilized to make the 4% PFA in 1X PBS reagent.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 1 | 2020 | Allen Institute for Brain Science 2020. 20% Paraformaldehyde in 5X PBS. protocols.io dx.doi.org/10.17504/protocols.io.bg4zjyx6 | 2021-03-29 03:13:47 | |||
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Mouse Cardiac Perfusion Fixation and Brain Collection Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.be2djga6 | BICCN, Allen Institute for Brain Science | This protocol describes the procedures for intracardiac perfusion fixation of postnatal mice, including anesthesia, exsanguination, fixation, brain removal and post-fixation storage. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 4 | 2020 | Allen Institute for Brain Science 2020. Mouse Cardiac Perfusion Fixation and Brain Collection. protocols.io dx.doi.org/10.17504/protocols.io.be2djga6 | 2021-03-29 03:12:36 | |||
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Isolation of Nuclei from Adult Human Brain Tissue for 10x Genomics Platform Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bq64mzgw | BICCN, Allen Institute for Brain Science | Isolation of nuclei from frozen adult human brain tissue or thawed and microdissected brain tissue sections for RNA-seq analysis. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U01MH114812. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 1 | 2021 | Allen Institute for Brain Science 2021. Isolation of Nuclei from Adult Human Brain Tissue for 10x Genomics Platform. protocols.io dx.doi.org/10.17504/protocols.io.bq64mzgw | 2021-03-29 03:12:38 | |||
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cDNA Synthesis Resource Report Resource Website |
Allen Institute for Brain Science | 10.17504/protocols.io.bgnbjvan | BICCN, Allen Institute for Brain Science | This protocol describes the synthesis of first-strand cDNA from total RNA using the Invitrogen SuperScript III Reverse Transcriptase system.Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. | Allen Institute | 4 | 2020 | Allen Institute for Brain Science 2020. cDNA Synthesis. protocols.io dx.doi.org/10.17504/protocols.io.bgnbjvan | 2021-03-29 03:12:43 |
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