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Authors: Matthew Sullivan, Sarah Schwenck, Cristina Howard-Varona, Vinh Dang
Group: VERVE Net, Sullivan Lab
Summary: Bacterial viruses (phages) influence global biogeochemical cycles by modulating bacterial mortality, metabolic output and evolution. However, our understanding of phage infections is limited by few methods and environmentally-relevant model systems. Prior work showed that Cellulophaga baltica phage ϕ38:1 infects its original host lytically, and an alternative host either delayed lytically or lysogenically. Here we investigate these infections through traditional and marker-based approaches, and introduce geneELISA for highthroughput examination of phage‒host interactions.
Proper citation: Matthew Sullivan, Sarah Schwenck, Cristina Howard-Varona, Vinh Dang 2016. geneELISA for highthroughput examination of phage?host interactions. protocols.io dx.doi.org/10.17504/protocols.io.dmc42v Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: .
Proper citation: Matthew Sullivan 2016. 0.7% Agarose. protocols.io dx.doi.org/10.17504/protocols.io.c7bzim Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: This mixture is used with Cesium Chloride Dialysis for Viruses
Proper citation: Matthew Sullivan 2016. MTN100 with 5x NaCl. protocols.io dx.doi.org/10.17504/protocols.io.ded3a5 Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Roseobacter Screening Of Surface Waters For Viruses. protocols.io dx.doi.org/10.17504/protocols.io.dbt2nm Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Checking DNA Concentration with Agarose Gel. protocols.io dx.doi.org/10.17504/protocols.io.c35yq5 Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: For use in DNase I Treatment protocol for DNase inactivation
Proper citation: Matthew Sullivan 2015. EGTA tetrasodium salt. protocols.io dx.doi.org/10.17504/protocols.io.c3pymm Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Calculating burst size. protocols.io dx.doi.org/10.17504/protocols.io.ddm245 Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. DNA/RNA Extractions from Sterivex Filters. protocols.io dx.doi.org/10.17504/protocols.io.c4bysm Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Reverse Cross-linking Protocol. protocols.io dx.doi.org/10.17504/protocols.io.c6bzam Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. YTSS Medium. protocols.io dx.doi.org/10.17504/protocols.io.dbq2mv Copy
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