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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
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Fluorescence Titering Assay for Lentivirus Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.4xrgxm6 | This protocol is for running a fluorescence titering assay for lentivirus. To see the full abstract and additional resources, visit the Addgene protocol page.Sample Data | Addgene | https://www.addgene.org/protocols/fluorescence-titering-assay/ | 1 | 2019 | Addgene The Nonprofit Plasmid Repository 2019. Fluorescence Titering Assay for Lentivirus. protocols.io dx.doi.org/10.17504/protocols.io.4xrgxm6 | 2021-03-29 03:08:41 | |||
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Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning) Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.bawnifde | This protocol describes plasmid cloning by restriction enzyme digest (aka subcloning). To see the full abstract and additional resources, please visit the Addgene protocol page. | Addgene | https://www.addgene.org/protocols/subcloning/ | 2 | 2019 | Addgene The Nonprofit Plasmid Repository 2019. Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning). protocols.io dx.doi.org/10.17504/protocols.io.bawnifde | 2021-03-29 03:08:02 | |||
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Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning) Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.47ugznw | This protocol describes plasmid cloning by restriction enzyme digest (aka subcloning). To see the full abstract and additional resources, please visit the Addgene protocol page. | Addgene | https://www.addgene.org/protocols/subcloning/ | 1 | 2019 | Addgene The Nonprofit Plasmid Repository 2019. Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning). protocols.io dx.doi.org/10.17504/protocols.io.47ugznw | 2021-03-29 03:11:18 | |||
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Colony Formation Titering Assay for Lentivirus Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.47mgzk6 | This protocol is for running a colony formation titering assay for Lentivirus. To see the full abstract and additional resources, visit the Addgene protocol page.Sample Data | Addgene | https://www.addgene.org/protocols/colony-formation-titering-assay/ | 1 | 2019 | Addgene The Nonprofit Plasmid Repository 2019. Colony Formation Titering Assay for Lentivirus. protocols.io dx.doi.org/10.17504/protocols.io.47mgzk6 | 2021-03-29 03:08:48 | |||
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Generating Stable Cell Lines with Lentivirus Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.4xtgxnn | This protocol is for generating stable cell lines with lentivirus. To see the full abstract and additional resources, please visit the Addgene protocol page.Sample Data | Addgene | https://www.addgene.org/protocols/generating-stable-cell-lines/ | 1 | 2019 | Addgene The Nonprofit Plasmid Repository 2019. Generating Stable Cell Lines with Lentivirus. protocols.io dx.doi.org/10.17504/protocols.io.4xtgxnn | 2021-03-29 03:08:54 | |||
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Restriction Digest of Plasmid DNA Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.5jeg4je | Please see the Addgene website for additional details. | Addgene | http://www.addgene.org/plasmid_protocols/restriction_digest/ | 2 | 2019 | Addgene The Nonprofit Plasmid Repository 2019. Restriction Digest of Plasmid DNA. protocols.io dx.doi.org/10.17504/protocols.io.5jeg4je | 2021-03-29 03:09:16 | |||
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AAV Production in HEK293T Cells Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.bawpifdn | This protocol is used for AAV production in HEK293T cells. To see the full abstract and additional resources, visit the Addgene protocol page.Sample Data | Addgene | https://www.addgene.org/protocols/aav-production-hek293-cells/ | 2 | 2019 | Addgene The Nonprofit Plasmid Repository 2019. AAV Production in HEK293T Cells. protocols.io dx.doi.org/10.17504/protocols.io.bawpifdn | 2021-03-29 03:09:17 | |||
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Polymerase Chain Reaction (PCR) Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.4wggxbw | This protocol is for performing Polymerase Chain Reaction (PCR). To see the full abstract and additional resources, visit https://www.addgene.org/protocols/pcr/.Basic PCR ProgramInitial Denaturation for at : This initiation step heats the double stranded DNA template strand to the point where the strands start denaturing and the hydrogen bonds are broken between the nucleotide base pairs.Denature at : Continued denaturation of double stranded DNA.Anneal primers for at : The forward and reverse primers are stable within this temperature range to anneal to each of the single stranded DNA template strands. The DNA polymerase is also stable enough to now bind to the primer DNA sequence.Extend DNA for at : The Taq polymerase has an optimal temperature around - so this step enables the DNA polymerase to synthesize and elongate the new target DNA strand accurately and rapidly.Repeat steps 2-5, 25-30 times.Final Extension for at : A final extension to fill-in any protruding ends of the newly synthesized strands. | Addgene | https://www.addgene.org/protocols/pcr/ | 1 | 2020 | Addgene The Nonprofit Plasmid Repository 2020. Polymerase Chain Reaction (PCR). protocols.io dx.doi.org/10.17504/protocols.io.4wggxbw | 2021-03-29 03:07:31 | |||
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Fluorescence Titering Assay for Lentivirus Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.bawtifen | This protocol is for running a fluorescence titering assay for lentivirus. To see the full abstract and additional resources, visit the Addgene protocol page.Sample Data | Addgene | https://www.addgene.org/protocols/fluorescence-titering-assay/ | 2 | 2019 | Addgene The Nonprofit Plasmid Repository 2019. Fluorescence Titering Assay for Lentivirus. protocols.io dx.doi.org/10.17504/protocols.io.bawtifen | 2021-03-29 03:10:42 | |||
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LB Agar Plates Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.cdzs75 | The following protocol is for making LB agar plates for the purpose of bacterial selection (500mL of LB agar makes about 25 LB agar plates). Please see the Addgene website for additional details. | Addgene | https://www.addgene.org/plasmid_protocols/bacterial_plates/ | 1 | 2014 | Addgene The Nonprofit Plasmid Repository 2014. LB Agar Plates. protocols.io dx.doi.org/10.17504/protocols.io.cdzs75 | 2021-03-29 03:12:34 | |||
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Pouring LB Agar Plates - Chem 584 Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository, Ken Christensen | 10.17504/protocols.io.bjvwkn7e | The following protocol is for making LB agar plates for the purpose of bacterial selection (500mL of LB agar makes about 25 LB agar plates). For the full abstract and additional resources, please visit https://www.addgene.org/plasmid_protocols/bacterial_plates/ | Addgene, Brigham Young University | https://www.addgene.org/plasmid_protocols/bacterial_plates/ | 1 | 2020 | Addgene The Nonprofit Plasmid Repository, Ken Christensen 2020. Pouring LB Agar Plates - Chem 584. protocols.io dx.doi.org/10.17504/protocols.io.bjvwkn7e | 2021-03-29 03:13:03 | |||
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General Transfection Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.bawuifew | This protocol is for general transfection. To see the full abstract and additional resources, visit the Addgene protocol page.Sample Data | Addgene | https://www.addgene.org/protocols/transfection/ | 2 | 2019 | Addgene The Nonprofit Plasmid Repository 2019. General Transfection. protocols.io dx.doi.org/10.17504/protocols.io.bawuifew | 2021-03-29 03:13:01 | |||
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General Transfection Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.4xqgxmw | This protocol is for general transfection. To see the full abstract and additional resources, visit the Addgene protocol page.Sample Data | Addgene | https://www.addgene.org/protocols/transfection/ | 1 | 2019 | Addgene The Nonprofit Plasmid Repository 2019. General Transfection. protocols.io dx.doi.org/10.17504/protocols.io.4xqgxmw | 2021-03-29 03:13:12 | |||
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Inoculating a Liquid Bacterial Culture Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.5abg2an | This protocol is for inoculating a liquid bacterial culture. To see the full abstract and additional resources, visit https://www.addgene.org/protocols/inoculate-bacterial-culture/. | Addgene | https://www.addgene.org/protocols/inoculate-bacterial-culture/ | 1 | 2020 | Addgene The Nonprofit Plasmid Repository 2020. Inoculating a Liquid Bacterial Culture. protocols.io dx.doi.org/10.17504/protocols.io.5abg2an | 2021-03-29 03:14:14 | |||
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Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.4g5gty6 | This protocol is for creating bacterial glycerol stocks for long-term storage of plasmids. To see the full abstract and other resources, visit the Addgene protocol page. | Addgene | https://www.addgene.org/protocols/create-glycerol-stock/ | 1 | 2019 | Addgene The Nonprofit Plasmid Repository 2019. Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids. protocols.io dx.doi.org/10.17504/protocols.io.4g5gty6 | 2021-03-29 03:14:26 | |||
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Cloning shRNA Oligos into pLKO.1 Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.cdus6v | This is the protocol accompanying the "pLKO.1 – TRC Cloning Vector". For information about the PLKO.1-TRC cloning vector and tips on designing shRNA oligos for pLKO.1 see Addgene's website: http://www.addgene.org/tools/protocols/plko/ | Addgene | http://www.addgene.org/tools/protocols/plko/ | 1 | 2014 | Addgene The Nonprofit Plasmid Repository 2014. Cloning shRNA Oligos into pLKO.1. protocols.io dx.doi.org/10.17504/protocols.io.cdus6v | 2021-03-29 03:14:25 | |||
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AAV Production in HEK293T Cells Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.47igzke | This protocol is used for AAV production in HEK293T cells. To see the full abstract and additional resources, visit the Addgene protocol page.Sample Data | Addgene | https://www.addgene.org/protocols/aav-production-hek293-cells/ | 1 | 2019 | Addgene The Nonprofit Plasmid Repository 2019. AAV Production in HEK293T Cells. protocols.io dx.doi.org/10.17504/protocols.io.47igzke | 2021-03-29 03:14:32 | |||
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RNA Extraction Without a Kit Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.beabjaan | Coronavirus Method Development Community | This protocol describes RNA extraction without a kit. To see the full abstract and additional resources, visit https://www.addgene.org/protocols/kit-free-rna-extraction/ | Addgene | https://www.addgene.org/protocols/kit-free-rna-extraction/ | 1 | 2020 | Addgene The Nonprofit Plasmid Repository 2020. RNA Extraction Without a Kit. protocols.io dx.doi.org/10.17504/protocols.io.beabjaan | 2021-03-29 03:14:31 | ||
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COVID-19 Addgene Operating Procedures Resource Report Resource Website |
Joanne Kamens, Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.bk9vkz66 | Re-Entering Labs Post COVID-19 Shutdown | Addgene, Addgene | 2 | 2020 | Joanne Kamens, Addgene The Nonprofit Plasmid Repository 2020. COVID-19 Addgene Operating Procedures. protocols.io dx.doi.org/10.17504/protocols.io.bk9vkz66 | 2021-03-29 03:14:42 | ||||
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Generating Stable Cell Lines with Lentivirus Resource Report Resource Website |
Addgene The Nonprofit Plasmid Repository | 10.17504/protocols.io.bawvife6 | This protocol is for generating stable cell lines with lentivirus. To see the full abstract and additional resources, please visit the Addgene protocol page.Sample Data | Addgene | https://www.addgene.org/protocols/generating-stable-cell-lines/ | 2 | 2019 | Addgene The Nonprofit Plasmid Repository 2019. Generating Stable Cell Lines with Lentivirus. protocols.io dx.doi.org/10.17504/protocols.io.bawvife6 | 2021-03-29 03:15:02 |
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