Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Syngenta drug screen Resource Report Resource Website |
Ida Barlow, Adam Mcdermott-Rouse, Luigi Feriani | 10.17504/protocols.io.9vqh65w | Behavioural Genomics | Protocol for preparing worms, preparing drug plates, dispensing worms with wormsorter and tracking on Hydra rigs | Imperial College London, Imperial College London, Imperial College London | 1 | 2020 | Ida Barlow, Adam Mcdermott-Rouse, Luigi Feriani 2020. Syngenta drug screen. protocols.io dx.doi.org/10.17504/protocols.io.9vqh65w | 2021-03-29 03:09:54 | |||
|
Menthol protocol to bleach Aiptasia Resource Report Resource Website |
Virginia Weis | 10.17504/protocols.io.immcc46 | Aiptasia-Symbiodiniaceae Model System | Method to chemically bleach Aiptasia in small volume dishes. | Integrative Biology, Oregon State University | 1 | 2018 | Virginia Weis 2018. Menthol protocol to bleach Aiptasia. protocols.io dx.doi.org/10.17504/protocols.io.immcc46 | 2021-03-29 03:09:54 | |||
|
Universal sandwich ELISA for investigating the binding of avian immunoglobulins to Streptococcal protein-G (SpG) using anti-IgY-peroxidase conjugate. Resource Report Resource Website |
Angel Justiz-Vaillant, Monica F. Smikle | 10.17504/protocols.io.bjqvkmw6 | University of the West Indies, [email protected] | University of the West Indies St. Augustine, University of the West Indies. Mona Campus | 1 | 2020 | Angel Justiz-Vaillant, Monica F. Smikle 2020. Universal sandwich ELISA for investigating the binding of avian immunoglobulins to Streptococcal protein-G (SpG) using anti-IgY-peroxidase conjugate. . protocols.io dx.doi.org/10.17504/protocols.io.bjqvkmw6 | 2021-03-29 03:09:54 | ||||
|
CGAP MACS Live Dead Separation Resource Report Resource Website |
Adam Hunter | 10.17504/protocols.io.qz5dx86 | Human Cell Atlas Method Development Community | CGAP | 1 | 2018 | Adam Hunter 2018. CGAP MACS Live Dead Separation. protocols.io dx.doi.org/10.17504/protocols.io.qz5dx86 | 2021-03-29 03:09:53 | ||||
|
Quality control for metagenomics data Resource Report Resource Website |
Qi Wang | 10.17504/protocols.io.be68jhhw | BGI, GIGA, GigaScience Press | Quality control for metagenomics data,including: remove low quality reads and host contamination reads. | BGI | 1 | 2020 | Qi Wang 2020. Quality control for metagenomics data. protocols.io dx.doi.org/10.17504/protocols.io.be68jhhw | 2021-03-29 03:09:54 | |||
|
Electroporation protocol for Vibrio natriegens Resource Report Resource Website |
Josef Hoff | 10.17504/protocols.io.u3geyjw | Electroporation protocol Weinstock paper:Matthew T Weinstock, Eric D Hesek,Christopher M Wilson, Daniel G GibsonVibrio natriegens as a fast-growing host for molecular biology Nature Methods volume 13, pages 849–851 (2016)To prepare before:recovery medium(BHI + v2 salts (204 mM NaCl, 4.2 mM KCl, 23.14mM MgCl2), and 680 mM sucrose) sterile filtration * i have a box of aliquots in the freezer and pre heat them before use-A vial of competent cells is retrieved from storage at −80 °C and allowed to thaw on ice.-Plasmid DNA and electrocompetent cells are combined and gently mixed in a chilled 1.5-mL microcentrifuge tube. -The cell–DNA suspension is transferred to a chilledelectroporation cuvette with a 0.1-cm gap size.-Cells are electroporated with 900V (in our Electroportor we cant set other parameters ) * in the Weinstock paper they recommend depending on the strain 700-900V, 25 μF and 200 Ω-Cells are immediatelyrecovered in 500 μL preheated (50°C) recovery medium and transferred to a 1,5-mL tube. * we preheat the media to 50°C because the recovery media is cooled down by pipetting and up taking the chilled cells from the cold cuvettes-The cells are recovered by incubating at 37 °C for 1.5h. ( also put the agar plates for preheating in the incubator at 37°C )-The cells are centrifuged down for one mintute at 3000g. The supernant is then discanted.- the pellet is resuspendet in the leftover oft he media and plated out on warm agar plates containing appropriate antibiotic. * for Chloramphenicol 2µg/mL ; for Kanamycine 200µg/mL; for Carbenicillin 200µg/mL-The plates are incubated for several hours or overnight at 37 °C for colonies to appear. | Phillips University | 1 | 2018 | Josef Hoff 2018. Electroporation protocol for Vibrio natriegens. protocols.io dx.doi.org/10.17504/protocols.io.u3geyjw | 2021-03-29 03:09:54 | ||||
|
Propagation/Amplification of Lytic Agent Resource Report Resource Website |
Mathias Middelboe, Amy M. Chan, and Sif K. Bertelsen | 10.17504/protocols.io.dq85zv | VERVE Net | For use in "Isolation of cyanophages by liquid enrichment assay" | Manual of Aquatic Viral Ecology, Manual of Aquatic Viral Ecology, Manual of Aquatic Viral Ecology | 1 | 2016 | Mathias Middelboe, Amy M. Chan, and Sif K. Bertelsen 2016. Propagation/Amplification of Lytic Agent. protocols.io dx.doi.org/10.17504/protocols.io.dq85zv | 2021-03-29 03:09:54 | |||
|
High resolution labeling of vagal efferent fibers using Dextran-Biotin with counterstaining Resource Report Resource Website |
Deborah Jaffey, Terry Powley, Jennifer Mcadams, Robert Phillips | 10.17504/protocols.io.2iqgcdw | SPARC | This protocol describes the methods used to trace and enable morphometric quantification of preganglionic efferent neurites in the rat stomach. A mixture of dextran conjugates was injected into the dorsal motor nucleus of the vagal nerve (dmnX) of young adult Sprague-Dawley rats and after a survival period of 19 days for optimal tracer transport, stomachs were removed and processed as whole mounts. ABC-DAB was used to create a permanent gold-brown stain of all labeled efferent neurites. Subgroups of samples were also counterstained with either the panneuronal chromogen cuprolinic blue or with nNOS antibodies and steel gray chromogen to label nitrergic cells. | Purdue University, Purdue University, Purdue University, Purdue University | 1 | 2019 | Deborah Jaffey, Terry Powley, Jennifer Mcadams, Robert Phillips 2019. High resolution labeling of vagal efferent fibers using Dextran-Biotin with counterstaining. protocols.io dx.doi.org/10.17504/protocols.io.2iqgcdw | 2021-03-29 03:09:54 | |||
|
Quantitative analysis method of mRNA expression using dual RNA in situ hybridization by comparison with housekeeping genes Resource Report Resource Website |
Byung Joon Seung | 10.17504/protocols.io.badcia2w | In retrospective formalin-fixed paraffin-embedded (FFPE) samples, RNA quality of FFPE tissues showed variation following the storage period and fixation process. So we suggest quantitative analysis by dual detection of target mRNA and housekeeping genes in one section and calculated the target gene/housekeeping gene ratio by two open-source image analysis programs. This assay potentially allows for reliable quantification of mRNA expression levels in retrospective FFPE samples. | Seung B, Cho S, Kim S, Lim H, Sur J (2020) Quantitative analysis of HER2 mRNA expression by RNA in situ hybridization in canine mammary gland tumors: Comparison with immunohistochemistry analysis. PLoS ONE 15(2): e0229031. doi: 10.1371/journal.pone.0229031 | Konkuk University | https://doi.org/10.1371/journal.pone.0229031 | 1 | 2020 | Byung Joon Seung 2020. Quantitative analysis method of mRNA expression using dual RNA in situ hybridization by comparison with housekeeping genes. protocols.io dx.doi.org/10.17504/protocols.io.badcia2w | 2021-03-29 03:09:51 | ||
|
Quick Protocol for Monarch® Plasmid Miniprep Kit (NEB #T1010) Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.nytdfwn | New England Biolabs (NEB) | This is the quick version of the Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010). For the full protocol, please click here. | New England Biolabs | https://www.neb.com/protocols/2015/12/08/quick-protocol-for-monarch-plasmid-miniprep-kit-t1010 | 2 | 2018 | New England Biolabs 2018. Quick Protocol for Monarch® Plasmid Miniprep Kit (NEB #T1010). protocols.io dx.doi.org/10.17504/protocols.io.nytdfwn | 2021-03-29 03:09:51 | ||
|
NEB 3X SDS Sample Loading Buffer Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.crcv2v | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/1/01/01/immunoprecipitation-using-protein-ag-magnetic-beads | 1 | 2015 | New England Biolabs 2015. NEB 3X SDS Sample Loading Buffer. protocols.io dx.doi.org/10.17504/protocols.io.crcv2v | 2021-03-29 03:09:51 | |||
|
microfluidic sars cov 2 lamp protocol Resource Report Resource Website |
Monica Simion, Alexandru Salceanu | 10.17504/protocols.io.bkjjkukn | Coronavirus Method Development Community, covid19-test, XPRIZE Rapid Covid Testing | IMT, IMT | 1 | 2020 | Monica Simion, Alexandru Salceanu 2020. microfluidic sars cov 2 lamp protocol. protocols.io dx.doi.org/10.17504/protocols.io.bkjjkukn | 2021-03-29 03:09:53 | ||||
|
Chelex DNA isolation for quick plant genotyping Resource Report Resource Website |
Magdalena Julkowska | 10.17504/protocols.io.pdudi6w | Quick and dirty, but very very fast protocol for DNA isolation. It works beautifully if you have plenty of lines to genotype - example T-DNA insertion lines for validation of your favourite gene. | King Abdullah University of Science and Technology | 1 | 2019 | Magdalena Julkowska 2019. Chelex DNA isolation for quick plant genotyping. protocols.io dx.doi.org/10.17504/protocols.io.pdudi6w | 2021-03-29 03:09:51 | ||||
|
CGAP Human Lung Dissociation - Tissue Stability Study Resource Report Resource Website |
Anna Wilbrey-Clark, Adam Hunter | 10.17504/protocols.io.34kgquw | Human Cell Atlas Method Development Community | Sanger Institute, Cambridge, HCA, CGAP | 1 | 2019 | Anna Wilbrey-Clark, Adam Hunter 2019. CGAP Human Lung Dissociation - Tissue Stability Study. protocols.io dx.doi.org/10.17504/protocols.io.34kgquw | 2021-03-29 03:09:53 | ||||
|
Protocol for LEO filter sample DNA extractions using PowerWater kit Resource Report Resource Website |
MoBio PowerWater instructions amended with specific instructions | 10.17504/protocols.io.h75b9q6 | Procedure for DNA extractions using PowerWater DNA extraction kit from filtered LEO seepage water samples and prelimnary QC by nanodrop | 1 | 2017 | MoBio PowerWater instructions amended with specific instructions 2017. Protocol for LEO filter sample DNA extractions using PowerWater kit. protocols.io dx.doi.org/10.17504/protocols.io.h75b9q6 | 2021-03-29 03:09:53 | |||||
|
Lysis Buffer (10mM Tris-HCl, 2mM EDTA, 1% SDS) Resource Report Resource Website |
Sarah Hessen-Schmidt | 10.17504/protocols.io.fjqbkmw | University of Southern California | 1 | 2016 | Sarah Hessen-Schmidt 2016. Lysis Buffer (10mM Tris-HCl, 2mM EDTA, 1% SDS). protocols.io dx.doi.org/10.17504/protocols.io.fjqbkmw | 2021-03-29 03:09:50 | |||||
|
DNA extraction from FTA Classical Card Resource Report Resource Website |
Adebambo, A. O. and Ajibike, A. B. | 10.17504/protocols.io.ihvcb66 | Federal University of Agriculture, Abeokuta, Ogun State, Federal University of Agriculture, Abeokuta, Ogun State, Federal University of Agriculture, Abeokuta, Ogun State | 1 | 2017 | Adebambo, A. O. and Ajibike, A. B. 2017. DNA extraction from FTA Classical Card. protocols.io dx.doi.org/10.17504/protocols.io.ihvcb66 | 2021-03-29 03:09:50 | |||||
|
BioBrick E0546 Destination Reaction Resource Report Resource Website |
New England Biolabs | 10.17504/protocols.io.cpmvk5 | New England Biolabs (NEB) | New England Biolabs | https://www.neb.com/protocols/1/01/01/digestion-protocol-e0546 | 1 | 2015 | New England Biolabs 2015. BioBrick E0546 Destination Reaction. protocols.io dx.doi.org/10.17504/protocols.io.cpmvk5 | 2021-03-29 03:09:50 | |||
|
UC Davis - Glucagon Resource Report Resource Website |
Peter Havel | 10.17504/protocols.io.63ihgke | Mouse Metabolic Phenotyping Centers | Glucagon is a 29 amino acid polypeptide processed from proglucagon in pancreatic alpha cells. In intestinal L-cells proglucagon is cleaved into glicentin, corresponding to proglucagon residues no 1-69. Glicentin can further be processed into oxyntomodulin, corresponding to proglucagon residues no 33-69. These peptides are released simultaneously upon stimulation. Moreover, a fragment of glucagon corresponding to its Cterminal part (residues no 19-29), also designated mini-glucagon, is reported to be present in the pancreas in low amounts compared to the total glucagon content. In general, glucagon has an effect opposite that of insulin, i.e. it raises blood glucose levels. It causes the liver to convert glycogen into glucose, which is then released into the blood stream. With longer stimulation, glucagon action in the liver results in a glucose-sparing activation of free fatty acid oxidation and production of ketones. During hypoglycaemia, glucagon secretion offers a protective feedback mechanism, defending the organism against damaging effects of glucose deficiency in the brain and nerves. | RRID:AB_2783839 | University of California, Davis | https://mmpc.org/shared/document.aspx?id=264&docType=Protocol | 2 | 2019 | Peter Havel 2019. UC Davis - Glucagon. protocols.io dx.doi.org/10.17504/protocols.io.63ihgke | 2021-03-29 03:09:53 | |
|
Cultivation / Spore Production for Alternaria Solani Resource Report Resource Website |
Remco Stam | 10.17504/protocols.io.fmibk4e | Stam Lab | Protocol for the cultivatio of Alternaria Solani.This protocol has been used for many years at the chair of Phytopathology at the Technical University of Munich.SNA Medium was first described by Nirenberg in 1981. | Technische Universität München | 1 | 2016 | Remco Stam 2016. Cultivation / Spore Production for Alternaria Solani. protocols.io dx.doi.org/10.17504/protocols.io.fmibk4e | 2021-03-29 03:09:51 |
Can't find your Protocol?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific protocol and you know the DOI of the protocol already, it's easier to enter a DOI to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your protocol in the search results, please help us by adding it into the system — it's easy. Create and publish your protocols at Protocols.io.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
