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Authors: Gabriel Y. K. Ganyaglo, Nessa Ryan, Joonhee Park, A. T. Lassey
Summary: Background Vesicovaginal fistula is a debilitating condition resulting from prolonged obstructed labor. Globally, at least 2 million women are estimated to be living with fistula with about 50,000 to 100,000 new cases each year. More than 90% of the global burden of fistula is in sub-Saharan Africa and South East Asia. Traditional management of fistula requires surgical repair. However, many women either do not have access to surgery, or access to surgery is delayed. These categories of patients have few or no options to control the constant urinary leakage.  The vaginal menstrual cup is an insertable medical device approved for use in Europe and North America to collect menstrual flow. Because it collects fluid drainage from the vagina, it could also be used to control urine leakage in women with vesicovaginal fistula.  While surgical management of fistula would remain the gold standard in treatment, the menstrual cup could be an alternative for women who do not have access to surgery or are poor surgical candidates.General Aim To evaluate the menstrual cup for short-term non-surgical management of vesicovaginal fistula.Methodology This study will examine the reduction in urinary leakage for women with vesicovaginal fistula who use the cup over a 2 hour period. Fistula patients recruited after community durbars  will be invited to the hospital and offered enrollment. They will wear a sanitary pad for 2 hours and the pad will be weighed. The patients will then receive counseling on how to insert the cup in the vagina and will wear both the device and a sanitary pad for 2 hours. The second pad will also be weighed. The amount of urine leaked with and without the cup will be compared using a paired t-test. Addtionally, the women will answer a short questionnaire on the acceptability of the cup, and a physical exam will be carried out to assess safety with cup use. A purposeful sample of the patients will be recruited for in-depth interviews to share their experiences of coping with VVF and their acceptability of the menstrual cup device.Expected Outcome The knowledge gained from this study may offer alternative temporizing management options for fistulas, especially for women who fail or are awaiting surgery, women who cannot access surgery, or are poor surgical candidates.

Proper citation: Gabriel Y. K. Ganyaglo, Nessa Ryan, Joonhee Park, A. T. Lassey 2018. Feasibility and Acceptability of the Menstrual Cup for Non-Surgical Management of Vesicovaginal Fistula among Women at a Health Facility in Ghana. protocols.io https://dx.doi.org/10.17504/protocols.io.sjfecjn Copy   

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Authors: Kiichi Hirota, Yoshiyuki Matsuo

Proper citation: Kiichi Hirota, Yoshiyuki Matsuo 2018. Measurement of insulin concentration. protocols.io https://dx.doi.org/10.17504/protocols.io.v63e9gn Copy   

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Authors: Morgan Colp
Group: Protist Research to Optimize Tools in Genetics (PROT-G)
Summary: Isolation of single Acanthamoeba castellanii cells from agar plates for establishment of monoclonal cultures. This protocol can be useful for isolating clones of a mixed wild-type population or of transformants. After isolation, growth can be re-established in typical Neff liquid medium.

Proper citation: Morgan Colp 2020. Single cell isolation and monoclonal culture establishment of Acanthamoeba castellanii using migration on agar plates. protocols.io https://dx.doi.org/10.17504/protocols.io.bc3eiyje Copy   

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Authors: Xiaohuan Sun, Yuehua Hu, Zewei Song
Summary: Library construcion steps following MGIEasy universal DNA library prep set user manual started from end repair and A-tailing to single strand circularizaiton in chaptor 3. 500 ng of gDNA is used in this Library Construction Protocol. The steps were described bellow. https://en.mgitech.cn/Uploads/Temp/picture/20191023/5db00efef2bd7.pdf

Proper citation: Xiaohuan Sun, Yuehua Hu, Zewei Song 2021. Library construction of metabarcoding at DNBSEQ-G400 with MGIEasy universal DNA library prep Kit. protocols.io https://dx.doi.org/10.17504/protocols.io.bn95mh86 Copy   

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Authors: Kenneth Schackart, Kattika Kaarj
Group: 481b Laboratory
Summary: This protocol details how to stain mammalian cells cultured on a 96-well plate. Actin filaments, focal adhesion sites (as indicated by the presence of vinculin), and nuclei will be stained.

Proper citation: Kenneth Schackart, Kattika Kaarj 2019. Mammalian Cell Staining. protocols.io https://dx.doi.org/10.17504/protocols.io.x95fr86 Copy   

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Authors: Ehtesham Mofiz
Group: GigaScience Press

Proper citation: Ehtesham Mofiz 2016. Draft genome assembly using parasitic mite population NGS DNA sample from mites extracted from host wound environment. protocols.io https://dx.doi.org/10.17504/protocols.io.exwbfpe Copy   

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Authors: Mingxin Guo

Proper citation: Mingxin Guo 2017. H2O2 content measurement . protocols.io https://dx.doi.org/10.17504/protocols.io.jqhcmt6 Copy   

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Authors: Chin-Ying Chen, Wei Sheng Huang, Hui-Chuen Chen, Chin-Hao Chang, Long-Teng Lee, Heng-Shuen Chen, Yow-Der Kang, Wei-Chu Chie, Chyi-Feng Jan, Wei-Dean Wang, Jaw-Shiun Tsai

Proper citation: Chin-Ying Chen, Wei Sheng Huang, Hui-Chuen Chen, Chin-Hao Chang, Long-Teng Lee, Heng-Shuen Chen, Yow-Der Kang, Wei-Chu Chie, Chyi-Feng Jan, Wei-Dean Wang, Jaw-Shiun Tsai 2020. Effect of a 90 g/day low-carbohydrate diet on glycemic control, small dense low density lipoprotein, and carotid intima-media thickness in type 2 diabetic patients: an 18month randomized controlled trial protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.bg3zjyp6 Copy   

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Authors: Audrey Lapinaite, Sam Sternberg
Group: The Center for Genome Editing and Recording
Summary: This is a protocol for in vitro transcription of DNA oligos by T7 polymerase from the Doudna Lab. 

Proper citation: Audrey Lapinaite, Sam Sternberg 2018. Protocol for in vitro transcription of DNA oligos by T7 polymerase. protocols.io https://dx.doi.org/10.17504/protocols.io.ssqeedw Copy   

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Authors: Michael Fero
Summary: A collection of protocols compatible with j5 and referenced in Springer-Verlag book chapter by M. Fero, N. Hillson and J.K. Craft.

Proper citation: Michael Fero 2019. j5 Protocols Collection. protocols.io https://dx.doi.org/10.17504/protocols.io.6ughetw Copy   

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Authors: David Dunigan and Irina Agarkova
Group: VERVE Net
Summary: For use in Polyacrylamide Gel System For Electrophoresis Of Proteins.

Proper citation: David Dunigan and Irina Agarkova 2016. 30.0% Acrylamide stock solution (30.0:0.8). protocols.io https://dx.doi.org/10.17504/protocols.io.eq2bdye Copy   

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Authors: Mariana Rius
Group: Protist Research to Optimize Tools in Genetics (PROT-G)

Proper citation: Mariana Rius 2017. Cell Preparation for Electroporation of Aurantiochytrium using Salt and Sucrose solutions. protocols.io https://dx.doi.org/10.17504/protocols.io.h65b9g6 Copy   

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Authors: Alba Balletbó
Group: iGEM Wageningen 2019

Proper citation: Alba Balletbó 2019. Taq PCR (Protocol for ZymoTaq™ PreMix). protocols.io https://dx.doi.org/10.17504/protocols.io.7cvhiw6 Copy   

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Authors: Eva Feldman
Group: Diabetic Complications Consortium
Summary: Summary:Plasma concentrations of thiobarbituric acid reactive substances (TBARS) are an index of lipid peroxidation and oxidative stress. The protocol describes how the DiaComp quantitates TBARS in the animal models.Diabetic Complication:

Proper citation: Eva Feldman 2019. Thiobarbituric acid reactive substances (TBARS) Assay. protocols.io https://dx.doi.org/10.17504/protocols.io.3sngnde Copy   

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Authors: Angel Justiz-Vaillant, Belkis Ferrer-Cosme
Group: University of the West Indies, [email protected]
Summary: IgG is a monomer with an approximate molecular weight of 146 Kd and a serum concentration of 9.0 mg/mL. It is synthesized mostly in the secondary immune response to pathogens. IgG can activate the classical pathway of the complement system, and it also is highly protective. The four subclasses of IgG include IgG1, IgG2, IgG3, and IgG4. IgG crosses the placentae, protecting the neonate from infectious diseases. [1]

Proper citation: Angel Justiz-Vaillant, Belkis Ferrer-Cosme 2020. ELISA for quantification of human immunoglobulin G (IgG) in serum or plasma.. protocols.io https://dx.doi.org/10.17504/protocols.io.bj7skrne Copy   

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Authors: Jessica J. Miciak, Jason Hirshberg and Fred Bunz, Fred Bunz
Summary: The diverse applications of mutant and recombinant adenoviruses demand facile methods for their generation. This protocol allows the user to rapidly assemble customized adenoviral genomes from high-copy plasmids in a two-step isothermal reaction. Following in vitro assembly, full-length genomes can be directly packaged in HEK293 cells. Crude preparations of infectious virus can be prepared in less than one week.

Proper citation: Jessica J. Miciak, Jason Hirshberg and Fred Bunz, Fred Bunz 2018. AdenoBuilder: modular assembly of recombinant adenovirus genomes from high-copy plasmids. protocols.io https://dx.doi.org/10.17504/protocols.io.qt5dwq6 Copy   

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Authors: Claudia Méndez, Maria Clara Duque, Yanira Romero, Julie Pérez, Omaira Rodríguez, Camilo A. Correa-Cárdenas, Maria Teresa Alvarado, Omar Cantillo-Barraza
Summary: This is the protocol to be used in the diagnosis of Chagas disease in Colombia, according to the algorithm suggested by Health and Social Protection Ministery with the National Health Institute.

Proper citation: Claudia Méndez, Maria Clara Duque, Yanira Romero, Julie Pérez, Omaira Rodríguez, Camilo A. Correa-Cárdenas, Maria Teresa Alvarado, Omar Cantillo-Barraza 2019. Protocol for detect Trypanosoma cruzi by indirect methods as IIF, ELISA and IHA from serological samples. protocols.io https://dx.doi.org/10.17504/protocols.io.62khgcw Copy   

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Authors: Janice E. Lawrence and Grieg F. Steward
Group: VERVE Net
Summary: OptiPrep gradients, as described for purifying viral isolates, may also be used for purifying marine viral assemblages. There are as yet, however, few descriptions of this application in the literature. CsCl gradients, on the other hand, have been used extensively for purifications of viral isolates and natural viral assemblages. Given the continued popularity of CsCl gradients, and their well-characterized performance, we present the following two-part purification and fractionation protocol for viruses in CsCl gradients. The first part involves relatively quick sedimentation through a step-gradient to remove the bulk of the contaminants. This is followed by a higher resolution continuous gradient to separate the viruses from residual contaminants and to separate viruses having differing buoyant densities from one another. We assume the starting material for the following procedure to be a concentrated suspension of viruses (whether filtered to remove cells or not).

Proper citation: Janice E. Lawrence and Grieg F. Steward 2015. Purification of viral assemblages from seawater in CsCl gradients. protocols.io https://dx.doi.org/10.17504/protocols.io.d2s8ed Copy   

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Authors: Andrea Sweigart
Group: Mimulus
Summary: Lactophenol-aniline blue recipe

Proper citation: Andrea Sweigart 2019. Lactophenol-aniline blue solution. protocols.io https://dx.doi.org/10.17504/protocols.io.baf4ibqw Copy   

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Authors: Ioannis Bellos
Summary: Preeclampsia represents a serious pregnancy complication with high fetal and maternal morbidity and mortality rates.Accurate prediction of the disease is essential to effectively discriminate the population of pregnant women to provide preventive measures, especially aspirin administration. Adipokines have been hypothesized to be involved in preeclampsia pathophysiology, due to their actions in regulating placental angiogenesis. Several observational studies have evaluated serum adipokine levels among preeclamptic women, although no consensus currently exists concerning their exact role in the disease. Therefore, a systematic review is planned aiming to accumulate all the available evidence in the field and assess whether serum adipokines can serve as useful predictive tools in preeclampsia.

Proper citation: Ioannis Bellos 2020. The role of serum adipokine levels in preeclampsia: a systematic review. protocols.io https://dx.doi.org/10.17504/protocols.io.bbipikdn Copy   

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