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Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. One-step growth curves for Cellulophaga phages. protocols.io dx.doi.org/10.17504/protocols.io.ddh235 Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: This mixture is is used with Cesium Chloride Dialysis for Viruses
Proper citation: Matthew Sullivan 2016. MTN100 with 3x NaCl. protocols.io dx.doi.org/10.17504/protocols.io.dee3bd Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: The following are media recipes necessary for isolating and maintaining Synechococcus isolates.
Proper citation: Matthew Sullivan 2016. Media recipes for Synechococcus isolates. protocols.io dx.doi.org/10.17504/protocols.io.c9jz4m Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. One-Step Growth Curves for Cyanophages. protocols.io dx.doi.org/10.17504/protocols.io.dan2dd Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: For Transcriptomics During One-Step Growth Curves for Cellulophaga Phages protocol.
Proper citation: Matthew Sullivan 2016. Non-Centrifuged Plaque Assay Sample Steps. protocols.io dx.doi.org/10.17504/protocols.io.der3d5 Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: For Transcriptomics During One-Step Growth Curves for Cellulophaga Phages protocol.
Proper citation: Matthew Sullivan 2016. RNA Samples Steps. protocols.io dx.doi.org/10.17504/protocols.io.dev3e5 Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Colony PCR Protocol. protocols.io dx.doi.org/10.17504/protocols.io.c6czav Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Cellulophaga Media Recipes. protocols.io dx.doi.org/10.17504/protocols.io.dez3f5 Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Marine Basal Media. protocols.io dx.doi.org/10.17504/protocols.io.dcc2sv Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: .
Proper citation: Matthew Sullivan 2016. NaCl Sodium Chloride Buffer. protocols.io dx.doi.org/10.17504/protocols.io.c64zgv Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Cellulophaga 38:1 Lytic Gene PCR. protocols.io dx.doi.org/10.17504/protocols.io.dfz3p5 Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: For One-step growth curves for Cellulophaga phages protocol.
Proper citation: Matthew Sullivan 2016. Non-Centrifuged Sample Steps. protocols.io dx.doi.org/10.17504/protocols.io.ddk24v Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Plaque assay protocol for Cellulophaga. protocols.io dx.doi.org/10.17504/protocols.io.ddg23v Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: Last optimized January 2006, updated by Matt on March 16 2009
Proper citation: Matthew Sullivan 2016. Large Volume Marine Cyanophage Phage Purification. protocols.io dx.doi.org/10.17504/protocols.io.c3iykd Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. FISH Protocol for FISH & FLOW. protocols.io dx.doi.org/10.17504/protocols.io.dfe3jd Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Proper citation: Matthew Sullivan 2016. Pseudoalteromonas Media Recipes. protocols.io dx.doi.org/10.17504/protocols.io.de23gd Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: Sequence for barcoded oligos for linker amplified libraries. Numbers 1 to 29 were developed at the Tucson Marine Phage Lab dervied from MID 1 to 14 recommended by Roche.
Proper citation: Matthew Sullivan 2016. Barcode Oligos for Linker Amplified DNA. protocols.io dx.doi.org/10.17504/protocols.io.ddp25m Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: Version 3This protocol describes the use of Cesium Chloride to purify viruses with an optional dialysis step to remove the CsCl. DNase I treatment, CsCl purification, and sucrose purification methods were compared using replicated viral metagenomics in Hurwitz et al. 2012.References:Hurwitz, B.L., Deng, L., Poulos, B.T., & Sullivan, M.B. (2012). Evaluation of methods to concentrate and purify ocean virus communities through comparative, replicated metagenomics. Environ Microbiol. 15(5), 1428–1440. doi:10.1111/j.1462-2920.2012.02836.x.
Proper citation: Matthew Sullivan 2016. Cesium Chloride Dialysis for Viruses. protocols.io dx.doi.org/10.17504/protocols.io.c7jzkm Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: For use in the protocol "Large Volume Marine Cyanophage protocols"
Proper citation: Matthew Sullivan 2016. Microbubbling technique. protocols.io dx.doi.org/10.17504/protocols.io.egkbbuw Copy
Authors: Matthew Sullivan
Group: VERVE Net, Sullivan Lab
Summary: For use in "One-step growth curve for Cyanophage"
Proper citation: Matthew Sullivan 2016. Time 0 growth reading using a plate reader. protocols.io dx.doi.org/10.17504/protocols.io.dw47gv Copy
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