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Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Glucose tolerance test measures systemic clearance of glucose following an intraperitoneal bolus injection of 20% dextrose. This experiment measures insulin sensitivity in awake mice assuming that there are no alterations in the animal’s pancreatic -cell function and insulin secretion. Insulin sensitivity is altered in obese mice.
Proper citation: Jason Kim 2019. U Mass - Glucose Tolerance Test. protocols.io https://dx.doi.org/10.17504/protocols.io.xxafpie Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of HDL-cholesterol reflect cholesterol metabolism and are associated with cardiovascular disease.
Proper citation: Jason Kim 2019. U Mass - Cholesterol (HDL). protocols.io https://dx.doi.org/10.17504/protocols.io.xt6fnre Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment provides the quantification of multiple hormones using multiplexed-Luminex technology based on beads containing specific antibodies. Sample matrices include plasma, serum, tissue culture supernatants, and tissue or cell lysates. Serum glucagon levels reflect glucagon secretion and pancreatic α-cell function. Serum glucagon levels regulate hepatic glucose production and glucose homeostasis. Serum glucagon levels are altered in obesity, insulin resistance, and counterregulatory stress response.
Proper citation: Jason Kim 2019. U Mass - Glucagon. protocols.io https://dx.doi.org/10.17504/protocols.io.xw3fpgn Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:
Whole body glucose turnover and hepatic glucose production rates are measured at basal state using an intravenous infusion of labeled glucose in awake mice. Whole body glucose turnover and hepatic glucose production regulate basal glucose levels and are altered in obesity.
Proper citation: Jason Kim 2019. U Mass - Basal glucose metabolism. protocols.io https://dx.doi.org/10.17504/protocols.io.xr8fm9w Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:Phloridzin is a potent inhibitor of renal glucose reabsorption and may be used to lower serum glucose levels. Phloridzin may be administered chronically to induce glycosuria and lower hyperglycemia in diabetic mice. Phloridzin may be administered acutely to reduce serum glucose levels for experiments in diabetic mice.
Proper citation: Jason Kim 2019. U Mass - Chronic/acute phloridzin treatment. protocols.io https://dx.doi.org/10.17504/protocols.io.xujfnun Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: A subcutaneous or intraperitoneal implantation of Alzet osmotic pump is used to chronically administer selected drug in mice. Chronic drug delivery may be used to examine intermediate to long-term effects of selected drug on obesity, insulin resistance, and metabolism.
Proper citation: Jason Kim 2019. U Mass - Chronic drug delivery. protocols.io https://dx.doi.org/10.17504/protocols.io.xuefnte Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:Glucose tolerance test with insulin secretion measures systemic clearance of glucose and systemic appearance of insulin following an intraperitoneal bolus injection of 20% dextrose. This experiment measures insulin sensitivity and insulin secretion (pancreatic -cell function) in awake mice assuming that there are no alterations in systemic insulin clearance. Insulin sensitivity and pancreatic -cell function are altered in obese mice.
Proper citation: Jason Kim 2019. U Mass - Glucose Tolerance Test with insulin secretion. protocols.io https://dx.doi.org/10.17504/protocols.io.xxbfpin Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
This experiment measures serum and plasma concentrations of non-esterified fatty acids using a 96-well kit. The experiment involves a coupled reaction to measure non-esterified fatty acids (NEFA) which ultimately forms a purple product that absorbs light at 550nm. This allows the concentration of NEFA to be determined from the optical density measured at 540~550nm. Serum fatty acids levels reflect systemic lipid metabolism, lipid digestion/absorption, and lipid clearance. Serum fatty acids levels are altered in obesity, insulin resistance, and type 2 diabetes.
Proper citation: Jason Kim 2019. U Mass - Non-esterified fatty acids. protocols.io https://dx.doi.org/10.17504/protocols.io.x3hfqj6 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of total protein reflect systemic protein metabolism.
Proper citation: Jason Kim 2019. U Mass - Total protein. protocols.io https://dx.doi.org/10.17504/protocols.io.x5gfq3w Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers, Metabolomics Protocols & Workflows
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Triglyceride levels may be measured in serum, plasma, and tissue samples. Serum and tissue triglyceride levels are affected by alterations in systemic lipid metabolism, lipid digestion/absorption, and lipid clearance. Serum and tissue triglyceride levels are altered in obesity, insulin resistance, type 2 diabetes, alcoholic steatohepatitis, non-alcoholic fatty liver disease, and non-alcoholic steatohepatitis.
Proper citation: Jason Kim 2019. U Mass - Triglyceride. protocols.io https://dx.doi.org/10.17504/protocols.io.x5pfq5n Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer.
Proper citation: Jason Kim 2019. U Mass - Amylase. protocols.io https://dx.doi.org/10.17504/protocols.io.xq4fmyw Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum uric acid levels are affected by alterations in systemic protein and nitrogen metabolism. Serum uric acid levels are altered in kidney failure and renal complications of diabetes.
Proper citation: Jason Kim 2019. U Mass - Uric Acid. protocols.io https://dx.doi.org/10.17504/protocols.io.x5sfq6e Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum alanine transferase levels reflect liver function.
Proper citation: Jason Kim 2019. U Mass - Alanine Transferase. protocols.io https://dx.doi.org/10.17504/protocols.io.xpmfmk6 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
This experiment provides the quantification of multiple hormones using multiplexed-Luminex technology based on beads containing specific antibodies. Sample matrices include plasma, serum, tissue culture supernatants, and tissue or cell lysates. Leptin is an adipocyte-derived hormone (adipokine) that regulates feeding behavior (hunger/satiety). Serum leptin levels are altered in obesity, insulin resistance, and type 2 diabetes.
Proper citation: Jason Kim 2019. U Mass - Leptin. protocols.io https://dx.doi.org/10.17504/protocols.io.x23fqgn Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Glucose uptake in individual organs can be measured using a bolus injection of 2-deoxy-D-[1-14C] glucose, a non-metabolizable glucose analog, and by determining labeled metabolite levels in select tissues. Insulin resistance is characterized by reduced glucose metabolism and develops in obese mice.
Proper citation: Jason Kim 2019. U Mass - Organ-specific glucose uptake. protocols.io https://dx.doi.org/10.17504/protocols.io.x4cfqsw Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:Chronic indwelling catheter is placed in the jugular vein for intravenous infusion and injection of drug, hormones, and metabolites. Surival surgery is performed in anesthetized mice, and mice recover from surgery after 4~5 days.
Proper citation: Jason Kim 2019. U Mass - Surgery – jugular vein cannulation. protocols.io https://dx.doi.org/10.17504/protocols.io.x5cfq2w Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of C-reactive peptide reflect systemic inflammation and may be altered in obesity.
Proper citation: Jason Kim 2019. U Mass - C-reactive Peptide. protocols.io https://dx.doi.org/10.17504/protocols.io.xt5fnq6 Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Lipase is a gastrointestinal enzyme that processes fat/lipid digestion.
Proper citation: Jason Kim 2019. U Mass - Lipase. protocols.io https://dx.doi.org/10.17504/protocols.io.x3bfqin Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary: Summary:Hyperglycemic clamp measures insulin secretion and pancreatic -cell function in awake mice. Islet function is affected by obesity and insulin resistance, and abnormal islet function predisposes to the development of type 2 diabetes.
Proper citation: Jason Kim 2019. U Mass - Hyperglycemic clamp. protocols.io https://dx.doi.org/10.17504/protocols.io.xzafp2e Copy
Authors: Jason Kim
Group: Mouse Metabolic Phenotyping Centers
Summary:
Lipid metabolism is estimated by measuring systemic clearance of [1-14C] palmitate following a bolus injection in awake mice. Lipid metabolism is altered in obese mice.
Proper citation: Jason Kim 2019. U Mass - Lipid metabolism. protocols.io https://dx.doi.org/10.17504/protocols.io.x3gfqjw Copy
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