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| Name | Authors | DOI | Group |
Summary |
Associated Publications |
RRIDs used | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
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1X CviRI Assay Buffer Resource Report Resource Website |
DAVID DUNIGAN AND IRINA AGARKOVA | 10.17504/protocols.io.es2bege | VERVE Net | For use in CviRI Purification From XZ-6E Virus Infected NC64A Chlorella. | 1 | 2016 | DAVID DUNIGAN AND IRINA AGARKOVA 2016. 1X CviRI Assay Buffer. protocols.io dx.doi.org/10.17504/protocols.io.es2bege | 2021-03-29 03:14:01 | ||||
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Viral DNA Miniprep Procedure Resource Report Resource Website |
David Dunigan and Irina Agarkova | 10.17504/protocols.io.eq6bdze | VERVE Net | University of Nebraska-Lincoln | 1 | 2016 | David Dunigan and Irina Agarkova 2016. Viral DNA Miniprep Procedure. protocols.io dx.doi.org/10.17504/protocols.io.eq6bdze | 2021-03-29 03:14:03 | ||||
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CviRI Storage Buffer Resource Report Resource Website |
DAVID DUNIGAN AND IRINA AGARKOVA | 10.17504/protocols.io.eszbef6 | VERVE Net | For use in CviRI Purification From XZ-6E Virus Infected NC64A Chlorella. | 1 | 2016 | DAVID DUNIGAN AND IRINA AGARKOVA 2016. CviRI Storage Buffer. protocols.io dx.doi.org/10.17504/protocols.io.eszbef6 | 2021-03-29 03:14:13 | ||||
|
Gradient Recipes Resource Report Resource Website |
David Dunigan and Irina Agarkova | 10.17504/protocols.io.eufbetn | VERVE Net | The University of Nebraska-Lincoln | 1 | 2016 | David Dunigan and Irina Agarkova 2016. Gradient Recipes. protocols.io dx.doi.org/10.17504/protocols.io.eufbetn | 2021-03-29 03:14:11 | ||||
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Running gel buffer (10X) Resource Report Resource Website |
DAVID DUNIGAN AND IRINA AGARKOVA | 10.17504/protocols.io.ercbd2w | VERVE Net | For use in Polyacrylamide Gel System For Electrophoresis Of Proteins. | 1 | 2016 | DAVID DUNIGAN AND IRINA AGARKOVA 2016. Running gel buffer (10X). protocols.io dx.doi.org/10.17504/protocols.io.ercbd2w | 2021-03-29 03:15:03 | ||||
|
CviJI Purification From IL-3A Virus Infected NC64A Chlorella Resource Report Resource Website |
David Dunigan and Irina Agarkova | 10.17504/protocols.io.er3bd8n | VERVE Net | The University of Nebraska-Lincoln | 1 | 2016 | David Dunigan and Irina Agarkova 2016. CviJI Purification From IL-3A Virus Infected NC64A Chlorella. protocols.io dx.doi.org/10.17504/protocols.io.er3bd8n | 2021-03-29 03:15:09 | ||||
|
CviRI Buffer B Resource Report Resource Website |
DAVID DUNIGAN AND IRINA AGARKOVA | 10.17504/protocols.io.esxbefn | VERVE Net | For use in CviRI Purification From XZ-6E Virus Infected NC64A Chlorella. | 1 | 2016 | DAVID DUNIGAN AND IRINA AGARKOVA 2016. CviRI Buffer B. protocols.io dx.doi.org/10.17504/protocols.io.esxbefn | 2021-03-29 03:15:30 | ||||
|
PBCV-1 Virus Plaque Assay Resource Report Resource Website |
David Dunigan and Irina Agarkova | 10.17504/protocols.io.estbeen | VERVE Net | PBCV-1 is a large plaque virus (approximately 2-3 mm in diameter). The plaque size of the viruses reflects the length of the virus replication cycle. PBCV-1 has a life cycle of about 6-8 hours. When titering you should use plates that were poured no more than 1 day before the time of use. Variable plaque formation will result if fresh plates are not used. (This is similar to a phage phenomenon.) Pyrex petri dishes do give more uniform plaque formation versus the plastic, however this is not so critical as with slower growing NC64A viruses. | University of Nebraska-Lincoln | 1 | 2016 | David Dunigan and Irina Agarkova 2016. PBCV-1 Virus Plaque Assay. protocols.io dx.doi.org/10.17504/protocols.io.estbeen | 2021-03-29 03:09:58 | |||
|
Formulation of FES Medium Resource Report Resource Website |
David Dunigan and Irina Agarkova | 10.17504/protocols.io.eucbesw | VERVE Net | The University of Nebraska-Lincoln | 1 | 2016 | David Dunigan and Irina Agarkova 2016. Formulation of FES Medium. protocols.io dx.doi.org/10.17504/protocols.io.eucbesw | 2021-03-29 03:10:10 | ||||
|
DTT solution Resource Report Resource Website |
David Dunigan and Irina Agarkova | 10.17504/protocols.io.erabd2e | VERVE Net | University of Nebraska | 1 | 2016 | David Dunigan and Irina Agarkova 2016. DTT solution. protocols.io dx.doi.org/10.17504/protocols.io.erabd2e | 2021-03-29 03:10:25 | ||||
|
CviRI Assay Conditions Resource Report Resource Website |
DAVID DUNIGAN AND IRINA AGARKOVA | 10.17504/protocols.io.es3begn | VERVE Net | For use in CviRI Purification From XZ-6E Virus Infected NC64A Chlorella. | 1 | 2016 | DAVID DUNIGAN AND IRINA AGARKOVA 2016. CviRI Assay Conditions. protocols.io dx.doi.org/10.17504/protocols.io.es3begn | 2021-03-29 03:12:01 | ||||
|
Definitive guide to western blot Resource Report Resource Website |
David Dunigan and Irina Agarkova | 10.17504/protocols.io.eqwbdxe | VERVE Net | Western blotting uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis. The immunoassay uses a membrane made of nitrocellulose or PVDF (polyvinylidene fluoride). The gel is placed next to the membrane and application of an electrical current induces the proteins to migrate from the gel to the membrane. The membrane can then be further processed with antibodies specific for the target of interest, and visualized using secondary antibodies and detection reagents. | University of Nebraska-Lincoln | 1 | 2016 | David Dunigan and Irina Agarkova 2016. Definitive guide to western blot. protocols.io dx.doi.org/10.17504/protocols.io.eqwbdxe | 2021-03-29 03:08:30 | |||
|
Chlorella NC64A and Mictractinium Pbi virus Purification Resource Report Resource Website |
David Dunigan and Irina Agarkova | 10.17504/protocols.io.etfbejn | VERVE Net | University of Nebraska-Lincoln | 1 | 2016 | David Dunigan and Irina Agarkova 2016. Chlorella NC64A and Mictractinium Pbi virus Purification. protocols.io dx.doi.org/10.17504/protocols.io.etfbejn | 2021-03-29 03:11:21 | ||||
|
CviJI Storage Buffer Resource Report Resource Website |
DAVID DUNIGAN AND IRINA AGARKOVA | 10.17504/protocols.io.esnbede | VERVE Net | For use in CviJI Purification From IL-3A Virus Infected NC64A Chlorella. | 1 | 2016 | DAVID DUNIGAN AND IRINA AGARKOVA 2016. CviJI Storage Buffer. protocols.io dx.doi.org/10.17504/protocols.io.esnbede | 2021-03-29 03:11:17 | ||||
|
Methyltransferase Assay Resource Report Resource Website |
David Dunigan and Irina Agarkova | 10.17504/protocols.io.etjbekn | VERVE Net | University of Nebraska-Lincoln | 1 | 2016 | David Dunigan and Irina Agarkova 2016. Methyltransferase Assay. protocols.io dx.doi.org/10.17504/protocols.io.etjbekn | 2021-03-29 03:11:15 | ||||
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Cracking buffer, 4X (10 mL) Resource Report Resource Website |
DAVID DUNIGAN AND IRINA AGARKOVA | 10.17504/protocols.io.erdbd26 | VERVE Net | For use in Polyacrylamide Gel System For Electrophoresis Of Proteins. | 1 | 2016 | DAVID DUNIGAN AND IRINA AGARKOVA 2016. Cracking buffer, 4X (10 mL). protocols.io dx.doi.org/10.17504/protocols.io.erdbd26 | 2021-03-29 03:08:44 | ||||
|
Formulation of MBBM (Modified Bold's Basal Medium) Resource Report Resource Website |
David Dunigan and Irina Agarkova | 10.17504/protocols.io.etwbepe | VERVE Net | This medium is for growing Chlorella variabilis NC64A, Chlorella heliozoae SAG 3.83, and Coccomyxa C-169. | University of Nebraska | 1 | 2016 | David Dunigan and Irina Agarkova 2016. Formulation of MBBM (Modified Bold's Basal Medium). protocols.io dx.doi.org/10.17504/protocols.io.etwbepe | 2021-03-29 03:08:44 | |||
|
Extraction of DNA from Virus Resource Report Resource Website |
David Dunigan and Irina Agarkova | 10.17504/protocols.io.et4beqw | VERVE Net | University of Nebraska-Lincoln | 1 | 2016 | David Dunigan and Irina Agarkova 2016. Extraction of DNA from Virus. protocols.io dx.doi.org/10.17504/protocols.io.et4beqw | 2021-03-29 03:08:48 | ||||
|
RNA Isolation from Viral-infected Chlorella NC64A Resource Report Resource Website |
David Dunigan and Irina Agarkova | 10.17504/protocols.io.erqbd5w | VERVE Net | University of Nebraska-Lincoln | 1 | 2016 | David Dunigan and Irina Agarkova 2016. RNA Isolation from Viral-infected Chlorella NC64A. protocols.io dx.doi.org/10.17504/protocols.io.erqbd5w | 2021-03-29 03:08:57 | ||||
|
10X MOPS Buffer Resource Report Resource Website |
DAVID DUNIGAN AND IRINA AGARKOVA | 10.17504/protocols.io.etgbejw | VERVE Net | For use in Preparation of Denaturing Agarose Gel for RNA Analysis. | 1 | 2016 | DAVID DUNIGAN AND IRINA AGARKOVA 2016. 10X MOPS Buffer. protocols.io dx.doi.org/10.17504/protocols.io.etgbejw | 2021-03-29 03:07:38 |
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