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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
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  • Species:tetrahymena thermophila (facet)

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Organism Name Proper Citation Species Synonyms Notes Phenotype Affected Gene Genomic Alteration Catalog Number Background Database Database Abbreviation Availability Source References Alternate IDs Record Last Update Mentions Count
c∆SAI 2- 9-1-Mtt-DCL1-1
 
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RRID:TSC_SD02278 Tetrahymena thermophila homozygous homokaryon of ∆DCL1 (c∆SAI2 9-1) ,neo 3 was deleted in mac and .assorted to pm-s; with MTTi locus replaced with MTT1-DCL1 construct. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. DCL1 (TTHERM_00284230) Micronucleus: homozygous neo3 KO of DCL1, Macronucleus: homozygous ∆DCL1, neo 3 was deleted in mac and assorted to pm-s, MTT1-DCL1 replaced some of MTT1 SD02278 dcl1[∆?::neo3]/dcl1[∆?::neo3] (dcl1[∆?]; MTT1[∆::DCL1]; mp-?, ?) TSC, Tetrahymena Stock Center TSC PMID:15598983 2026-08-29 04:08:54 0
HTA3-G305A1
 
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RRID:TSC_SD02673 Tetrahymena thermophila HTA3 germline knockout heterokaryon. Transformed CU428 X B2086 with pQR22 (remove all coding by neo cassette). It can mate with G308A3 and G304A1, From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. HTA3 (TTHERM_00143660) Micronucleus: neo2 into HTAZ coding SD02673 htaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (mpr1-1/MPR1-1?; mp-r, VII) TSC, Tetrahymena Stock Center TSC PMID:12665578
PMID:11430834
2026-08-29 04:08:54 0
CU508
 
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RRID:TSC_SD00257 Tetrahymena thermophila SD00257 chx1-1/CHX1; MPR1/mpr1-1; H1(neo)/+ (chx1-1/CHX1; MPR1/mpr1-1; cy-r, mp-r, pm-r, II) TSC, Tetrahymena Stock Center TSC 2026-08-29 04:08:53 0
RNH1.3 sKO (7)-13-1
 
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RRID:TSC_SD02712 Tetrahymena thermophila Somatic KO of RNH1.3 , neo3, CU427 background. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. RNH13 (TTHERM_00283900) Macronucleus: Neo3 knockout of RNH1.3 SD02712 chx1-1/chx1-1 (rnh1.3-1[∆::neo3]; pm-r, VI) TSC, Tetrahymena Stock Center TSC 2026-08-29 04:08:53 0
IA298
 
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RRID:TSC_SD01227 Tetrahymena thermophila Ciliary rows helically wound around the cell. Expression is not temperature-sensitive. Note that this gene was published under the name TWI1, and later renamed SCR1 to avoid confusion with the piwi-homologue that had previously been named TWI1. Submitted by Joseph Frankel, University of Iowa SCR1 (screwy-1) SD01227 scr1-1/scri1-1 (scr1-1, II) TSC, Tetrahymena Stock Center TSC PMID:18658256 2026-08-29 04:08:54 0
CU5066
 
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RRID:TSC_SD01191 Tetrahymena thermophila SD01191 chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 5 (CHX1; pmr1-1; cy-s, pm-r, II) TSC, Tetrahymena Stock Center TSC 2026-08-29 04:08:54 0
CU428 XS49 (15)
 
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RRID:TSC_SD02280 Tetrahymena thermophila Complete somatic transformant with HTA1-neo2 replacing the HTA1(HTAX) gene in mac of CU428. (Has~60 bp deleted in HTA1 3’flanking at about 500 bp downstream of TGA) From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. HTA1 (TTHERM_00790790) Macronucleus: HTA1 with neo2 cassette in 3’ flank SD02280 mpr1-1/mpr1-1 (hta1[3’neo2]; pm-r, VII) TSC, Tetrahymena Stock Center TSC PMID:17242195 2026-08-29 04:08:53 0
BTGHB #2
 
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RRID:TSC_SD02047 Tetrahymena thermophila pBTGHB HA rescued GTUKO 5x6 progeny. WT GTU with HA tag@c-terminus; driven by MTT1 promoter. Needs Cd to induce. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. GTU1 (TTHERM_00079520) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with 3HA tags at c-teminus into the MTT1 locus, partial replacement. SD02047 gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU1, 3HAc)]; pm-r, ?) TSC, Tetrahymena Stock Center TSC PMID:16344310
PMID:12356864
2026-08-29 04:08:53 0
HTA1wt rescue Double knockout∆∆ 1-4(3)
 
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RRID:TSC_SD02169 Tetrahymena thermophila It is HTA1 WT somatic rescue strain of HTA1,HTA2 double knockout. Can mate with 1-5(12). From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500) Micronucleus: Neo 2 knockout of HTAX and HTA1 genes Macronucleus: WT HTAX SD02169 hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (HTA2 has neo2; pm-r, ?) TSC, Tetrahymena Stock Center TSC PMID:17242195
PMID:12665578
2026-08-29 04:08:54 0
HTZ1-B4a
 
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RRID:TSC_SD02687 Tetrahymena thermophila New Batch of G304A1xG311A1 ∆HTAZ knockout heterokaryon. Rescued with HTAZ Mating type not II,VI, or VII, Same with B3a. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. HTA3 (TTHERM_00143660) Micronucleus: Neo2 in HTAZ Macronucleus: HTAZ coding, SD02687 htaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (HTAZ/htaz[∆::neo2]; pm-r, mp-r, II) TSC, Tetrahymena Stock Center TSC PMID:12665578 2026-08-29 04:08:53 0
∆TBP
 
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RRID:TSC_SD02729 Tetrahymena thermophila Partial TATA-binding protein gene knockout with neo cassette,somatic. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. TBP1 (TTHERM_00575350) Macronucleus: Partial Neo KO of TBP1 SD02729 mpr1-1/mpr1-1 (TBP1/tbp1[∆?::neo2]; pm-r, VII) TSC, Tetrahymena Stock Center TSC PMID:8264641 2026-08-29 04:08:53 0
E-M
 
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RRID:TSC_SD02295 Tetrahymena thermophila E-M construct is used for somatic transformation From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. HHO1 (TTHERM_00823720) SD02295 mpr1-1/mpr1-1 (Circular permutation of HHO1 E5 charge patch, impossible to define in correct nomenclature, see paper.; pm-r, ?) TSC, Tetrahymena Stock Center TSC PMID:11972045 2026-08-29 04:08:54 0
LH2
 
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RRID:TSC_SD02696 Tetrahymena thermophila Transformation of CU428xB2086, complete knockout of mic histone linker gene with neo2 cassette inserted into Stu1 site. Paromomycin res. to 2 mg/ml Cell lines LH3 and LH6 can mate with each other. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. MLH1 (TTHERM_00471820) Macronucleus: Neo2 cassette inserted into coding of MLH1 SD02696 (mlh1[243::neo2]; pm-r, ?) TSC, Tetrahymena Stock Center TSC PMID:7606784 2026-08-29 04:08:54 0
1235D-2(BTU1)
 
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RRID:TSC_SD02212 Tetrahymena thermophila Progeny of DB6B x DB2A, transformed by BTU1-1235D mutation construct (E431, 432, 433, 435D) From the Gorovsky lab, University of Rochester. BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580) Micronucleus: knockout of btu1 with neo knockout of btu2 with bsr Macronucleus: BTU1 has only (E431, 432, 433, 435D) no WT BTU2 is KO SD02212 btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-? [E431D,E432D,E433D,E435D]; pm-r, ?) TSC, Tetrahymena Stock Center TSC PMID:10831613
PMID:11864572
2026-08-29 04:08:54 0
CDC5’ 480-GFP-CDC3’-neo3 (XS70)
 
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RRID:TSC_SD02576 Tetrahymena thermophila XS68 Somatic transformant obtained by shooting CU428 X B2086 exconjugant cells.WT gene CDC2 locus was partially replaced with GFP coding driven by CDC2 5’ flanking 480 bp region . Neo3 is in the CDC2 3’ flanking region. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. CDC2 (TTHERM_01207660) Macronucleus: CDC2 gene has 5’ flanking sequence of 480 bp and GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking. SD02576 ? (Partial replacement CDC2/cdc2-? CDC2 gene 5’ flanking sequence of 480 bp with GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking.; pm-r with Cd+2, ?) TSC, Tetrahymena Stock Center TSC PMID:17194754
PMID:15870266
2026-08-29 04:08:53 0
HTA3-T41B1
 
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RRID:TSC_SD02456 Tetrahymena thermophila G304A1xG311A1 ∆HTA3 knockout heterokaryon. Rescued with HTAZ mutated RRRRRK. Slow growth. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. HTA3 (TTHERM_00143660) Micronucleus: Neo2 in HTAZ Macronucleus: HTAZ coding replaced with mutations K4R, K7R, K10R, K13R, K16R. SD02456 htaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (htaz[∆::neo2]/htaz[K4R,K7R,K10R,K13R,K16R]; pm-r) TSC, Tetrahymena Stock Center TSC PMID:12665578
PMID:11430834
2026-08-29 04:08:54 0
B2086/fsNEO
 
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RRID:TSC_SD03307 Tetrahymena thermophila NEO coding sequence with two frameshift mutations in RPL29 vector (conferring resistance to Cyclohexamide, but remaining sensitive to paromomycin). The intention is this will provide the OLD MAC with a (defective) copy of the resistance gene, preventing its elimination in the NEW MAC after MIC transformation. From the Winey Lab at the University of Colorado Boulder. Macronucleus: RPL29::frame shift NEO SD03307 WT (WT; WT, ?) TSC, Tetrahymena Stock Center TSC PMID:23426847 2026-08-29 04:08:54 0
∆WAG1 HAT11 W7
 
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RRID:TSC_SD02736 Tetrahymena thermophila mates with ∆ WAG1 HAT11 W11 From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. WAG1 (TTHERM_00299879), TWI11 (TTHERM_00144830) Micronucleus: heterozygous KO of WAG1 and TWI11, TWI1 also HA tagged Macronucleus: KO of WAG1 with neo3, HA tagged TWI11 SD02736 WAG1/wag1[∆::neo3]; TWI11/twi11[neo3,HAn] (wag1[∆::neo3]; TWI11/HA tagged TWI11; pm-r, ?) TSC, Tetrahymena Stock Center TSC PMID:19596782 2026-08-29 04:08:54 0
BMCG- ∆DCL1-63
 
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RRID:TSC_SD01805 Tetrahymena thermophila ∆DCL1 homokaryon transformed with Bsr-MTT1-CNA1-GFP. CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cassette was inserted at the 5’F seq. of MTT1 locus. The construct was transformed into mac. Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed. CNA1 (TTHERM_00146340), DCL1 (TTHERM_00284230) Micronucleus: DCL1 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’ SD01805 dcl1[∆?::neo3]/dcl1[∆?::neo3] (dcl1[∆?::neo3]/dcl1[∆?::neo3]; MTT1/mtt1-?[5’ bsr1,∆,(::CNA1), GFPc]; bsr-r, pm-r, GFP tagged Cna1p, ?) TSC, Tetrahymena Stock Center TSC PMID:15598983
PMID:16738316
2026-08-29 04:08:53 0
CU427.4
 
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10+ mentions
RRID:TSC_SD00715 Tetrahymena thermophila Cycloheximide resistance functional heterokaryon.Regenerated micronucleus from CU427.2 by "star" mating. Non-star side maintained. SD00715 chx1-1/chx1-1 (CHX1; cy-s, VI) TSC, Tetrahymena Stock Center TSC PMID:730051
PMID:689366
PMID:411713
2026-08-29 04:08:54 17

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