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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 4 (CHX1; pmr1-1; cy-s, pm-r, II)
Proper citation: RRID:TSC_SD01084 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; mpr1-1/mpr1-1; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (CHX1[C3]; cy-s, ?)
Source References: PMID:8601476, PMID:8725229
Notes: This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, mp-r, pm-s, mat3.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01240 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 3 (CHX1; pmr1-1; cy-s, pm-r, II)
Proper citation: RRID:TSC_SD01003 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; disA/disA (CHX1; cy-s, tr, VII)
Proper citation: RRID:TSC_SD00155 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 4 (CHX1; pmr1-1; cy-s, pm-r, II)
Proper citation: RRID:TSC_SD01095 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (IV)
Proper citation: RRID:TSC_SD00283 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (pm-r, ?)
Affected Genes: HHO1 (TTHERM_00823720)
Notes: somatic gene replacement use A5 mutation to replace wild type HHOI gene neo cassette is at 3’ flanking region
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02222 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: MPR1/mpr1-1; JMJ2/jmj2-1[Δ21-672::neo2] (MPR1/mpr1-1; JMJ2/jmj2-1[Δ21-672::neo2]; mp-r, pm-r, ?)
Affected Genes: JMJ2 (TTHERM_00455500)
Genomic Alteration: Micronucleus: JMJ2 gene partially replaced by neo4-KanR2 cassette.
Notes: The knockout has been confirmed by 6-methylpurine and paromomycin resistance, but neither micronuclear nor macronuclear allele homozygosity has been verified.
Submitted by Robert Coyne, J. Craig Venter Institute
Proper citation: RRID:TSC_SD01775 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; gal1-1/gal1-1; TS11/TS11 or ts11-1/ts11-1; Nulli 1L,2R,3,5 (CHX1; GAL1?; mpr1-1; ts11-1; cy-s, gal?, mp-r, ts, IV)
Proper citation: RRID:TSC_SD00169 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; chx2-1/chx2-1; MPR1/MPR1 (CHX1; CHX2; cy-s, mp-r, ts, II)
Source References: PMID:730051
Proper citation: RRID:TSC_SD00047 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Del 1L (CHX1; cy-s, VI)
Notes: Deletion on left arm of chromosome 1.
Proper citation: RRID:TSC_SD00721 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: MPR1-1/mpr1-1; TWI7/twi7[∆?::neo3] (mpr1-1; mp-r, ?)
Affected Genes: TWI7 (TTHERM_00600450)
Genomic Alteration: Micronucleus: TWI7 partially replaced by neo3
Notes: heterozygote TWI7 KO heterokaryon. Partial TWI7 coding sequences were replaced by Neo3.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02869 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1/CHX1; chx2-1/chx2-1; MPR1/MPR1 (CHX2; MPR1; cy-s, mp-s, tr, III)
Source References: PMID:730051
Proper citation: RRID:TSC_SD00050 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/gal1-1; TS11/TS11 or ts11-1/ts11-1; Nulli 1L,2R,4L(p) (CHX1; GAL1?; mpr1-1; ts11-1; cy-s, gal?, mp-r, ts, IV)
Proper citation: RRID:TSC_SD00173 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (Mac∆TWI1::neo3, twi1[∆::neo3]; mpr1-1; pm-r, mp-r, ?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Macronucleus: Coding region of TWI1 replaced by neo3
Source References: PMID:12297043
Notes: B2086 X CU428 were transformed with TWI1 KO construct (whole coding was replaced by MTT-neo-BTU) and endogenous TWI1 gene was pushed out. Somatic transformant
this strain can mate with B2086, cu427, TWI1-KO2,-KO10.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02113 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Del 3R (MPR1; mp-s, VII)
Notes: Deletion on right arm of chromosome 3.
Proper citation: RRID:TSC_SD00970 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (II)
Proper citation: RRID:TSC_SD00299 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (mp-r, II)
Affected Genes: HTA1 (TTHERM_00790790) + HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTAX and HTA1 genes
Source References: PMID:12665578, PMID:11430834
Notes: G115B5xG204F2. HTA1, HTA2 double germline knockout heterokaryon. Can mate with G4B1G6A, G4B1G10A
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01393 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (btu1-? [∆2,3,4] ; BTU2/btu2-?[∆::neo1], ?)
Affected Genes: BTU (TTHERM_00348510)1, BTU2 (TTHERM_00836580)
Genomic Alteration: Macronucleus: BTU1 has the REI removed from the MREI at start of protien, BTU2 partically replaced by btu2::neo1
Notes: Original strain B6-D(VB1 cells with BTU1,MREI has REI deleted but rest of BTU1 is WT, the VB1 mutation L350 to M350 is back to L350 so no longer hyper sensitive to taxol) This cell was somatically transformed. Need to check if all BTU1 is replaced.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02483 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cnjB[∆?::neo3]/ cnjB[∆?::neo3] (cnjB[∆?::neo3]; pm-r, ?)
Affected Genes: CnjB (TTHERM_01091290)
Genomic Alteration: Micronucleus: neo3 replaces part of CnjB coding Macronucleus: neo3 replaces part of CnjB coding
Source References: PMID:19596782
Notes: Homozygote CnjB KO Homokaryon. Partial cnjB coding sequences were replaced by neo3.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02762 Copy
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