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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00031262
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Source References: EMPTY
Synonyms: unc-119(ed3) III; vhIs24.
Alternate IDs: WB-STRAIN:QR109, CGC_QR109
Notes: vhIs24 [vha-6p::GFP::rab-5 Q78L + Cbr-unc-119(+)]. Large endosomes in the intestinal cells.
Proper citation: RRID:WB-STRAIN:WBStrain00031262 Copy
http://www.wormbase.org/db/get?name=WBStrain00031261
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Source References: EMPTY
Synonyms: unc-119(ed3) III; vhIs6.
Alternate IDs: WB-STRAIN:QR47, CGC_QR47
Notes: vhIs6 [vha-6p::mCherry::tbc-2(R689K) + Cbr-unc-119(+)]. mCherry::TBC-2(R689K) is expressed in the intestine.
Proper citation: RRID:WB-STRAIN:WBStrain00031261 Copy
http://www.wormbase.org/db/get?name=WBStrain00031325
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Source References: PMID:37620393
Synonyms: unc-119(ed3) III; rrrIs1.
Alternate IDs: WB-STRAIN:RAF1, CGC_RAF1
Notes: Made_by: Biedermann/Senften|"rrrIs1 [pie-1p::GFP::Histone H2B::cye-1 3'UTR + unc-119(+)]. Slightly Unc."
Proper citation: RRID:WB-STRAIN:WBStrain00031325 Copy
http://www.wormbase.org/db/get?name=WBStrain00031314
Source Database: WormBase (WB)
Affected Genes: WBGene00001864(him-5)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00001864(him-5), WBGene00006843(unc-119)
Availability: available
Source References: EMPTY
Synonyms: unc-119(ed3) III; him-5(e1490) V; rdIs26.
Alternate IDs: WB-STRAIN:RA334, CGC_RA334
Notes: Made_by: Ed Large|"Mutagen:Gamma radiation"|"rdIs26 [R08E3.4::GFP + unc-119(+)]. Construct contains ~5 kb upstream of R08E3.4A. Superficially wild-type. Reference: Large and Mathies (2010) Dev Biol 339(1):51-64."
Proper citation: RRID:WB-STRAIN:WBStrain00031314 Copy
http://www.wormbase.org/db/get?name=WBStrain00031313
Source Database: WormBase (WB)
Affected Genes: WBGene00001864(him-5)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00001864(him-5), WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: unc-119(ed3) III; him-5(e1490) V; rdIs25.
Alternate IDs: WB-STRAIN:RA333
Notes: Mutagen:Gamma radiation|"rdIs25 [pRA400; R08E3.4::GFP + unc-119(+)]. Superficially wild-type. Reference: Large and Mathies (2010) Dev Biol 339(1):51-64."
Proper citation: RRID:WB-STRAIN:WBStrain00031313 Copy
http://www.wormbase.org/db/get?name=WBStrain00031312
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Source References: EMPTY
Synonyms: unc-119(ed3) III; rdIs24.
Alternate IDs: WB-STRAIN:RA332, CGC_RA332
Notes: Made_by: Ed Large|"rdIs24 [F12E12.5::GFP + unc-119(+)]. Spontaneous integrant of UL1189. Expresses GFP in somatic gonad during L2-L3 stages. Reference: Large EE, Mathies LD. Dev Biol. 2010 Mar 1;339(1):51-64."
Proper citation: RRID:WB-STRAIN:WBStrain00031312 Copy
http://www.wormbase.org/db/get?name=WBStrain00054628
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: Cbr-unc-119(stIs20000) III; stIs20022.
Notes: Made_by: Waterston lab|"stIs20022 [Cbr-his-72::GFP::pie-1 3'UTR + Cbr-unc-119(+)]. C. briggsae with fluorescently labeled (GFP) histones. Reference: Zhou Z, et al. Genetics. 2010 Mar; 184(3): 853863. doi: 10.1534/genetics.109.110270 PMID: 20008572."|"[2023-10-27T18:59:54.666Z WBPerson51134] New Strain: C. briggsae. CGC_data_submission"
Proper citation: RRID:WB-STRAIN:WBStrain00054628 Copy
http://www.wormbase.org/db/get?name=WBStrain00054699
Source Database: WormBase (WB)
Affected Genes: WBGene00000962(dhc-1)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00000962(dhc-1), WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: dhc-1(lt45[dhc-1::GFP]) I; ltSi953 II; unc-119(ed3) III.
Notes: ltSi953 [mec-18p::vhhGFP4::ZIF-1::operon-linker::mKate2::tbb-2 3'UTR + Cbr-unc-119(+)] II. GFP tag inserted into the C-terminus of the endogenous dhc-1 locus using CRISPR-Cas9 engineering. Tissue-specific expression of GFP nanobody::ZIF-1 fusion promotes ubiquitylation and subsequent degradation of GFP-tagged dhc-1 protein in touch receptor neurons. Touch receptor neurons are red labeled with mKate2. Reference: Development. 2017 Jul 15;144(14):2694-2701. PMID: 28619826.
Proper citation: RRID:WB-STRAIN:WBStrain00054699 Copy
http://www.wormbase.org/db/get?name=WBStrain00054684
Source Database: WormBase (WB)
Affected Genes: WBGene00001423(fib-1)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00001423(fib-1), WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: muIs252 II; unc-119(ed3) III; fib-1(mu498[wrmScarlet11::fib-1]) V.
Notes: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Homozygous viable. Endogenously-tagged wrmScarlet11::linker::fib-1 generated via CRISPR/Cas9 insertion into parental strain CF4582. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628
Proper citation: RRID:WB-STRAIN:WBStrain00054684 Copy
http://www.wormbase.org/db/get?name=WBStrain00054685
Source Database: WormBase (WB)
Affected Genes: WBGene00001877(his-3)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00001877(his-3), WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: muIs252 II; unc-119(ed3) III; his-3(mu500[his-3::wrmScarlet11(x3)]) V.
Notes: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Homozygous viable. Endogenously-tagged his-3::wrmScarlet11(x3) generated via CRISPR/Cas9 insertion of three wrmScarlet11 tags into the endogenous his-3 locus in parental strain CF4582. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628
Proper citation: RRID:WB-STRAIN:WBStrain00054685 Copy
http://www.wormbase.org/db/get?name=WBStrain00054680
Source Database: WormBase (WB)
Affected Genes: WBGene00003230(mex-5)|WBGene00004896(smu-2)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00003230(mex-5), WBGene00004896(smu-2), WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: cer227[mex-5p::SpG(smu-2 introns) + unc-119(+)] II; unc-119(ed3) III.
Notes: Made_by: Dmytro Kukhtar|"Missense mutations D1135L and S1136W, G1218K and E1219Q, and R1335Q and T1337R were introduced on the Cas9 gene at EG9615 strain, to cause endogenous expression of the Cas9 variant SpG. SpG is efficient for CRISPR on NGN PAM sites. Reference: Vicencio J, et al. Nature Communication, 2022. May 12;13(1):2601. doi: 10.1038/s41467-022-30228-4."
Proper citation: RRID:WB-STRAIN:WBStrain00054680 Copy
http://www.wormbase.org/db/get?name=WBStrain00054681
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)|WBGene00013025(vha-13)
Genomic Alteration: WBGene00006843(unc-119), WBGene00013025(vha-13)
Availability: unknown
Source References: EMPTY
Synonyms: muIs252 II; unc-119(ed3) III; vha-13(mu493[wrmScarlet11::vha-13]) V.
Notes: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Homozygous viable. Endogenously-tagged wrmScarlet11::vha-13 generated via CRISPR/Cas9 insertion into parental strain CF4582. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628
Proper citation: RRID:WB-STRAIN:WBStrain00054681 Copy
http://www.wormbase.org/db/get?name=WBStrain00054757
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: aSi13 II; unc-119(ed3) III.
Notes: aSi13 [lox2272 + loxN 3' (delta)Cbr-unc-119(+) + 3' (delta)mNeonGreen::PEST] aSi14[lox2272 + loxP 3 (delta)HygR + 3 (delta)mScarlet-I::PEST]II. Unc. Strain contains a set of dual specialized safe harbor transgene landing pads for integration of promoters: one driving mScarlet and rescuing hygromycin resistance upon integration, the other driving mNeonGreen and rescuing the unc phenotype upon integration. Reference: Stevenson ZC, et al. bioRxiv 2022.10.30.514301; doi: https:
Proper citation: RRID:WB-STRAIN:WBStrain00054757 Copy
http://www.wormbase.org/db/get?name=WBStrain00054881
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: unc-119(ed9) III; irSi24 IV.
Notes: irSi24 [pept-1::mCherry::H2B + Cbr-unc-119(+)] IV. Single-copy MosSCI insertion of pept-1 reporter into cxTi10882 site in LG IV. mCherry fluorescence exclusively in intestinal nuclei from the end of embryonic development through adulthood. Generated in N2 background. Reference: Maduro MF, et al. Dev Biol 2015 Aug 1;404(1):66-79. doi: 10.1016/j.ydbio.2015.04.025, PMID:25959238.
Proper citation: RRID:WB-STRAIN:WBStrain00054881 Copy
http://www.wormbase.org/db/get?name=WBStrain00054870
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: unc-119(ed3) III; bcSi69 IV; bcEx1368.
Notes: bcSi69 [hsp-16.41p::dCas9::SV40-NLS::HA::GS-linker::SV40-NLS::GFP::GFP::SV40-NLS::mai-2 3UTR + Cbr-unc-119(+)] IV. bcEx1368 [U6p::rrn-1(sgRNAs1-4 targeting the rrn-1 locus) + rol-6(su1006)]. Maintain at 15C. Pick Rollers to maintain. After heat shock, distinct spots of GFP fluorescence are visible in the nucleus. bcEx1368 array contains a PCR product of 4 different guide RNAs targeting rrn-1 (around 100 repeats of rrn-1 on LG I). Reference: Memar N, et al. In vivo labeling of endogenous genomic loci in C. elegans using CRISPR/dCas9. MicroPubl Biol. 2022 Dec 13;2022:10.17912/micropub.biology.000701. doi: 10.17912/micropub.biology.000701. PMID: 36606081; PMCID: PMC9807462.
Proper citation: RRID:WB-STRAIN:WBStrain00054870 Copy
http://www.wormbase.org/db/get?name=WBStrain00054897
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: unc-119(ed4) III; qyIs553
Notes: qyIs553 [lin-29p::ceGreenGlifon4000 +unc-119(+)]. Superficially wild-type strain expressing green glucose biosensor in the anchor cell. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617
Proper citation: RRID:WB-STRAIN:WBStrain00054897 Copy
http://www.wormbase.org/db/get?name=WBStrain00054981
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: ltSi220 I; ltSi1232 II; unc-119(ed3) III.
Notes: ltSi220 [mex-5p::GFP::tbb-2::operon-linker::mCherry::his-11 + Cbr-unc-119(+)] I. ltSi1232 [spd-2p::spd-5(S170A T178A T198A)::spd-5 3'UTR + Cbr-unc-119(+)] II. mCherry-labeled histones. GFP-labeled microtubules. Reference: Ohta M, et al. J Cell Biol. 2021 Feb 1;220(2):e202009083. doi: 10.1083/jcb.202009083. PMID: 33399854.
Proper citation: RRID:WB-STRAIN:WBStrain00054981 Copy
http://www.wormbase.org/db/get?name=WBStrain00054983
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: ltSi220 I; ltSi1561 II; unc-119(ed3) III.
Notes: ltSi220 [mex-5p::GFP::tbb-2::operon-linker::mCherry::his-11 + Cbr-unc-119(+)] I. ltSi1561 [spd-2p::spd-5(S170A T178A T198A S653A S658A)::spd-5 3'UTR + Cbr-unc-119(+)] II. mCherry-labeled histones. GFP-labeled microtubules. Reference: Ohta M, et al. J Cell Biol. 2021 Feb 1;220(2):e202009083. doi: 10.1083/jcb.202009083. PMID: 33399854.
Proper citation: RRID:WB-STRAIN:WBStrain00054983 Copy
http://www.wormbase.org/db/get?name=WBStrain00054987
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: ltSi264 II; unc-119(ed3) III.
Notes: ltSi264 [bub-1p::bub-1(re-encoded)::RFP + Cbr-unc-119(+)] II. BUB-1::RFP has been re-coded to be knl-1(RNAi) resistant. Reference: Pelisch F, et al. Mol Cell. 2017 Jan 5;65(1):66-77. doi: 10.1016/j.molcel.2016.11.001. PMID: 27939944|"Made_by: Arshad Desai lab"
Proper citation: RRID:WB-STRAIN:WBStrain00054987 Copy
http://www.wormbase.org/db/get?name=WBStrain00054966
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: EMPTY
Synonyms: ltSi597 I; unc-119(ed3) III.
Notes: ltSi597 [knl-1p::knl-1::mCherry::knl-1 3'UTR Cbr-unc-119(+)] I. mCherry labeled KNL-1. Reference: Hattersley et al., Dev Cell 2016 Sep 12;38(5):463-77. doi: 10.1016/j.devcel.2016.08.006. PMID: 27623381|"Made_by: Arshad Desai lab"
Proper citation: RRID:WB-STRAIN:WBStrain00054966 Copy
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