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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT2/hht2[3’neo2 ∆::(HHT3,GFPc)]; pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: C-terminal GFP-tag of HHT3, not sure if complete replacement, into HHT2 locus
Source References: PMID:16908532
Notes: Somatic replace HHT2 coding region with HHT3-GFP sequence. Not sure where the neo2 cassette is.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01896 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (twi11[∆?::neo3]; pm-r, VII)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Macronucleus: TWI11 with neo3
Notes: Somatic TWI 11 KO. Part of the coding sequence was replaced by neo3. In CU428
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02865 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (HTB1/htb1[∆::neo2]; htb2 [∆::neo2] assorted to pm-s so must have lost neo2; mp-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1
Source References: PMID:19822522
Notes: New batch, rescue of progeny h∆∆HTB86B4A6 and D2A2. Not mating type II,VI,VII.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02468 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,GFPc]; pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: C-terminal GFP- tag of HHT3, not sure if complete replacement
Source References: PMID:16908532
Notes: Somatic replace HHT3 gene with HHT3-GFP sequence. Neo2 in 5’ flanking of HHT3.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01897 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ∆hht1 & ∆hht2 ∆hhf1 ∆hhf2
hht1[∆,neo2]/hht1[∆,neo2];
hhf1[∆,neo]/hhf1[∆,neo]; hhf2,hht2[∆, neo2]/ hhf2,hht2[∆, neo2] (hht1[∆,neo2]/hhf1[∆,neo]/hhf2,hht2[∆, neo2]/ chimeric H3[H3(1-75) H3.3(76-90) H3(91-135)]
MTT1/mtt1-?[5’ neo2,∆,(::CNA1), GFPc]; pm-r, bsr-1, hybrid H3 replaces major H3.
In MTT1 locus,GFP tagged CNA1)
Affected Genes: CNA1 (TTHERM_00146340), HHT2 (TTHERM_00189180), HHT3 (TTHERM_00016170)
Genomic Alteration: Micronucleus: ∆hht1, ∆hht2 ∆hhf1, ∆hhf2
Macronucleus: hybrid H3 replaces major H3.
In MTT1 locus, GFP tagged CNA1
Source References: PMID:15701804
Notes: major h3, h4 germline KO cells were rescued with hy3. Hybrid H3 contruct with H3(1-75) H3.3 (76-90) H3 (91-135), then transformed with Bsr-mtt1-cna1-gfp construct.
CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cas.
Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01813 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; MPR1[C3]/MPR1[C3]; PMR1[C3]PMR1[C3]; MAT3/MAT3 (CHX1[C3]; cy-s, ?)
Source References: PMID:8601476, PMID:8725229
Notes: This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, mp-s, pm-s, mat3.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01265 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pmr1[C3]-1/pmr1[C3]-1;CHX1[C3]/CHX1[C3] (PMR1/PMR1 ;chx1-1/chx1-1; cy-r, pm-s, VII)
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01540 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (pm-r, II)
Affected Genes: TTHERM_00238830
Genomic Alteration: Macronucleus: V5 tagged PP36 protein
Source References: PMID:22426537
Notes: somatic mac transformation of B2086 with construct of PP36 (Protein phosphatase 36 kD MW, predicted TTHERM_00238830) V5 tag after ATG, with neo3 in reverse orientation -200 bases upstream of ATG. Localizes to nucleoli, not studied further.
Incomplete replacement, pushed to 3mg/ml pm with 0.5ug/ml Cd.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02233 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BTU1/btu1[∆,::H4-neo]; MPR1-1/mpr1-1
Affected Genes: BTU1 (TTHERM_00348510)
Genomic Alteration: Micronucleus: H4-neo replaces coding of BTU1, heterozygous
Source References: PMID:9037049, PMID:10831613
Notes: Heterozygous germline transformant of BTUI knockout. Derived from Cu428 X B2086 transformed w/ BHAB-1(construct). When crossed to CU427, gave cyr and pmr progeny.
Can mate to BIKO-2.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02753 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]; MTT1/mtt1-?[∆(::BTU1)HAc]; pm-r, ?)
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: knockout of btu1 with neo
knockout of btu2 with bsr Macronucleus: HA c-terminal tagged BTU1 into MTT1 locus
Source References: PMID:15254268, PMID:11864572, PMID:10831613
Notes: Progeny of DB6B x DB2A, transformed by BTU-HA TTMet construct. The transformants are dependent on Cadmium, 0.25ug/ml.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02236 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (pm-r, ?)
Affected Genes: RDR1 (TTHERM_00853150)
Genomic Alteration: Macronucleus: HA-FLAG tag of RDR1
Notes: Shot plasmid pRDP1::TAG-neo3 #110 into conjugating CU428XB2086 but only got somatic strains, slow growth.
No other information
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02512 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: sfr13-1[∆::NEO2] (sfr13-1[∆::NEO2]::SFR13[5'FLAG::BSR]::SFR13 3'; WT cell size and number of basal bodies., ?)
Affected Genes: SFR13 (TTHERM_00522830)
Genomic Alteration: Micronucleus: cen2-1[∆::NEO2] Macronucleus: FLAG-SFR13 rescue in two parts (see comments)
Notes: sfr13 complete null rescue. First a 5' part of the gene was introduced into the sfr13 locus with BSR selection that included a 5'FLAG tag. Second, the remainder of the gene was introduced to the KO locus with the 5' BSR insertion, and rescue of the small cell size phenotype was selected for.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03298 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ngoA[∆::neo3]/ngoA[∆::neo3] (ngoA[∆::neo3]
; pm-r, NgoA KO, ?)
Affected Genes: NgoA (TTHERM_00655890)
Genomic Alteration: Micronucleus: neo3 into NgoA coding Macronucleus: neo3 into NgoA coding
Source References: PMID:15870266
Notes: It’s a homozygous clone of KO of NgoA gene in both mac and mic grew up from sinle cell. the KO construct is: Neo gene driven by MTT promoter(2.5kb), and this is in opposite direction in NgoA flanking. NgoA KO-12 can mate with NgoA KO-7
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02488 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cnjB[∆?::neo3]/ cnjB[∆?::neo3]
(cnjB[∆?::neo3], ?)
Affected Genes: CnjB (TTHERM_01091290)
Genomic Alteration: Micronucleus: Neo3 replaces part of coding Macronucleus: Neo3 replaces part of coding
Source References: PMID:19596782
Notes: Homozygote CnjB KO Homokaryon. Partial cnjB coding sequences were replaced by neo3.
This strain was supposed to be pm-r but thawed pm-s.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02761 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi1[∆?::neo3]/twi1[∆?::neo3] (twi1[∆?::neo3]; eri1[∆?::neo3]; same as ∆Twi1)
Affected Genes: ERI1 (TTHERM_00501020)
Genomic Alteration: Micronucleus: TWI1 ko Macronucleus: twi1 kO and ERI1 KO
Notes: Eri1 KO construct was shot into twi1 KO homozygous homokaryon (Neo3 gene is lost in mac, strain is pm-s).
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02007 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (VI)
Notes: Collected by Paul Doerder on 8/26/2008 from the pond designated SG69-1 in Guys Mills, PA (lat. 41 37.174, long. -79 55.095, elevation 1549 ft, nearest road is Carpenter Road).
Geographical area: Western Pennsylvania
Collection number: 893
Has a micronucleus.
Cech's self-splicing intron is present. Cytochrome oxidase I haplotype = ANF1
Proper citation: RRID:TSC_SD01556 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 ko of HHT1 and HHT2/HHF2
Source References: PMID:16908532
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01955 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E443D,E445D E446D,E447D]; ATU1 with E443D, E445D,E446D,E447D or neo replacing coding of some ATU1 genes, pm-r, cy-s,mp-?
)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E443D, E445D, E446D, E447D or neo replacing coding of some ATU1 genes
Source References: PMID:10831613, PMID:9037049
Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pATU-4D derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and E443D, E445D,E446D,E447D
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01837 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: blt1-1[∆,neo3]/ blt1-1[∆,neo3]; MPR1-?/ MPR1-? (BLT1; mpr1-1; mp-r, ?)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Micronucleus: Neo3 replaces BLT coding
Notes: BLT germline heterokaryon, homozyous KO in mic, pm sensitive (120 ug/ml). Cd induced Neo cassette (1.2 ug/ml)--MTT promoter, Neo coding, BTU2 terminator. Construct pBLTMKO, Mates with BLTGL1-3,4,5 and 2-5.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02250 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Proper citation: RRID:TSC_SD01166 Copy
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