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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (htaz[∆::neo2]/htaz[K4R,K10R,K13R,K16R,K21R]; pm-r, ?)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: Neo2 in HTAZ Macronucleus: HTAZ coding replaced with mutations K4R, K10R, K13R, K16R, K21R
Source References: PMID:11430834, PMID:12665578
Notes: G304A1xG311A1 ∆HTA3 knockout heterokaryon. Rescued with HTAZ. mutated RKRRRR. Slow growth, ts at 40C
Proper citation: RRID:TSC_SD02076 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3];pmr1-1; MAT2; cy-s, pm-r, ?)
Source References: PMID:8601476, PMID:8725229
Notes: Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01300 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HTAY?/htay[∆::neo3]; MTT1/mtt1[5’neo2, ∆, 2HAn(::HTAY)]; pm-r, VII)
Affected Genes: H2A.Y (TTHERM_01079200)
Genomic Alteration: Macronucleus: Neo3 KO of HTAY(may be incomplete) and HTAY with two HA tags on the N-terminus inserted into MTT1 locus with neo2 cassete in 5’ flanking region
Source References: PMID:22426537
Notes: Transformed pXS83 (MTT-neo2-2HA-HTAY-MTT) into HTA.Y real somatic knockout strain in MTT1 locus.Selected in the presence of Cd +Pm. PCR shows very faint band of WT HTA.Y and WT MTT1 copy left (might come from mic).
Grow cells 0.5 ug/ml Cd and .5 mg/ml pm and increase that up to 6 mg/ml with passage.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01786 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (HTA3/hta3[∆::neo2], ?)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Macronucleus: neo2 into HTA3
Source References: PMID:8754831, PMID:8760889
Notes: CET of CU428 x B2086 with plamid XL203, HTA3 with neo2 cassette replacing coding
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02874 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wi1[∆::neo3]/twi1[∆::neo3] (twi1[∆::neo3] with rDNA::pD5H8-PDD1-GFP, ?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Micronucleus: Neo3 into TWI1, homozygous Macronucleus: TWI1 knockout
Source References: PMID:12297043
Notes: Homozygous homokaryon of TWI1 KO with PDD1-GFP expressed from the rDNA vector.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02758 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: sfr13-1[∆::NEO2] (sfr13-1[∆::NEO2]::SFR13[5'::BSR]::SFR13 3'; WT number of basal bodies (fewer cortical rows when analyzed)., ?)
Affected Genes: SFR13 (TTHERM_00522830)
Genomic Alteration: Micronucleus: cen2-1[∆::NEO2] Macronucleus: SFR13 rescue in two parts (see comments)
Source References: PMID:23426847
Notes: sfr13 complete null rescue. First a 5' part of the gene was introduced into the sfr13 locus with BSR selection. Second, the remainder of the gene was introduced to the KO locus with the 5' BSR insertion, and rescue of the small cell size phenotype was selected for.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03297 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: WAG1/wag1[∆:::neo3]; cnjB/cnjB[∆::neo3] (wag1[∆:::neo3] ; CnjB[∆::neo3]; pm-r, ?)
Affected Genes: WAG1 (TTHERM_00299879), cnjB (TTHERM_01091290)
Genomic Alteration: Micronucleus: heterozygous KO of WAG1 and CnjB Macronucleus: Complete KO of WAG1 and CnjB with neo3
Source References: PMID:19596782
Notes: somatic knock-outs of WAG1 and cnjB mates with ∆Wag1 ∆CnjB#16. Obtained by mating heteozygous WAG1 KO by heterozygous CnjB KO.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02121 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; pmr1-1/pmr1-1; MAT3/MAT3 (CHX1[C3];pmr1-1; MAT3; cy-s, pm-r, ?)
Source References: PMID:8725229, PMID:8601476
Notes: Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01271 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 ko of HHT1 and HHT2/HHF2
Source References: PMID:16908532
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series All the 10.* series are separate cell lines.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01959 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-?[5’neo2,∆,HAHAn(::TCD3)]; pm-r, VII)
Affected Genes: HPL4 (TTHERM_00585180)
Genomic Alteration: Macronucleus: Two HA tags after ATG of TCD3, neo2 in 5’ flank, inserted into MTT1 locus for over expression, need Cd
Notes: HA2-TCD2 overexpession from MTT1 locus, neo 2 upstream. HA-Tcd3p localizes to old mac and new mac.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02498 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (III)
Notes: Collected by Paul Doerder on 7/24/2009 from the pond designated Willard Pond in Antrim, NH (lat. 43 01.105, long. -72 01.262, elevation 1069 ft, nearest road is SR123, Willard Pnd Rd).
Geographical area: New England
Collection number: 1179
Has a micronucleus.
Proper citation: RRID:TSC_SD01561 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1[E445D]; pm-r, mp-?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E445D,
Source References: PMID:9037049, PMID:10831613
Notes: It is a progeny of conjugants of AAK02.7 X AAKO5.5 transformed with pD445 derived from pTUB100E3-PvuII (linearized) where the ATU1 gene is marked with Hae III and Pvu II sites and E445D. Full replacement.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02771 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: janA-1/janA-1; eja1-1/eja1-1; chx1-1/chx1-1 (janA-1; eja1-1; chx1-1; janA, eja1, cy-r, III)
Affected Genes: JANA (janus A) (originally reported as jan); EJA1 (enhancer of janus A); CHX1
Source References: PMID:28305307, PMID:1743393, PMID:448269
Notes: A mutation that causes the dorsal cell surface to be converted to a partial mirror-image of the ventral surface. Probably very similar to IA-220.
Proper citation: RRID:TSC_SD00631 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/Chx1-1[C3]; MAT2/MAT3 (chx1-1; MAT2; cy-r, IV)
Source References: PMID:10880474
Notes: Not useful for mapping loci on MIC chromosome 3R. One parent was missing this chromosome arm, so the progeny were not heterozygous.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01961 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ubc2[∆::neo2]/ubc2[∆::neo2] (?)
Affected Genes: UBC2 (TTHERM_00550720)
Genomic Alteration: Micronucleus: Neo2 KO of UBC2
Source References: PMID:19822522
Notes: UBC2 KO homozygous heterokaryon. Mates with ∆UBC2gl 1B2.8 and 1B2.10.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02536 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: eri1[∆::neo3]/eri1[∆::neo3]; mpr1-1/mpr1-1 (eri1[∆::neo3]; pm-r, ?)
Affected Genes: ERI1 (TTHERM_00501020)
Genomic Alteration: Micronucleus: ERI1 coding replaced by neo3 Macronucleus: ERI1 coding replaced by neo3
Notes: homozygous Er1 ko homokaryon ‘a’ can be mating with homozygous Er1 ko homokaryon ‘d’; coding sequences were replaced by Neo 3. Developmental delay.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02778 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 1L (CHX1; pmr1-1; cy-s, pm-r, II)
Notes: Deletion on left arm of chromosome 1.
Proper citation: RRID:TSC_SD00753 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-1[5’neo2,∆,HAc(::BLT11-4288,BTU1429-443)], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BLT1(AA 1-428) withBTU1 at C terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstreamin MTT1 5’ region
Notes: CU428 with BLT/BTU1 chimeric gene in MTT1 locus with HA tag. CU428 somatic transformant with pBbc-8 construct. BLT1(AA 1-428) with BTU1 at C-terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02261 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-1[5’neo2,∆,HAc(::BLT11-4288,BTU1429-443)], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BLT1(AA 1-428) withBTU1 at C terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstreamin MTT1 5’ region
Notes: CU428 with BLT/BTU1 chimeric gene in MTT1 locus with HA tag. CU428 somatic transformant with pBbc-8 construct. BLT1(AA 1-428) with BTU1 at C-terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02262 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht2[3’neo2, ∆::(HHT3,GFPc)]/hht2[3’neo2, ∆::(HHT3,GFPc)] (?HHT2/hht2[3’neo2 ∆::(HHT3,GFPc)], ?)
Affected Genes: HHT3 (TTHERM_00016170), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: C-terminal GFP- tag of HHT3, into HHT2 locus
Source References: PMID:16908532
Notes: germline H3.3 +GFP at H3.2, heterokaryon. lnon-star side of H3.3+GFP germline replacement at H3.2 locus
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02303 Copy
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