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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155260366
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 155260366
Notes: rat strain designed to express Cas9 under the control of the rat Ddx4 (Vasa) regulatory elements while also retaining expression of Ddx4. The strategy entailed insertion of a P2A sequence followed by human-codon-optimized Cas9 coding sequences with nuclear localization signals near the C-terminus of the rat Ddx4 (Vasa) gene. Animals express Cas9 in the testis in the spermatogonial layer and in meiosis stages with 4N DNA content. Strain catalyzes high levels of Active Genetic Gene conversion when paired with certain Active Genetic cassettes. ROBUST AND EFFICIENT ACTIVE GENETICS GENE CONVERSION IN THE RAT AND MOUSE Chenyen Lai, Oscar Alvarez, Kristen Read, Don van Fossan,Christopher M Conner, Shannon Xaing-Ru Xu, Dale O. Cowley, Valentino Gantz, David R. Webb, Kurt Jarnagin URL: Doi:10.1101/2022.08.30.505951
URL: Doi:10.1101/2022.08.30.505951 Donated to Rat Resource & Research Center
Proper citation: RRID:RGD_155260366 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155260368
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2022-10-06)
Alternate IDs: 155260368
Notes: rat strain in which the rat Cyp3a1 gene (Cyp3a23-3a1, RGD:628626) was inactivated by insertion of a human CYP3A4 minigene (Cyp3A4*) and the linked rat Cyp3a2 locus was simultaneously mutated by CRISPR-mediated indel introduction. Strain contains Indel 8 at the Rat 3A2 locus (55 kb away), The CYP3A4 minigene included a CRISPR/Cas9 gene drive element to enable super-Mendelian inheritance. Strain contains and Active Genetic cassette which encodes a gRNA targeting the Rat 3A1 and 3A2 translation initiation site. ROBUST AND EFFICIENT ACTIVE GENETICS GENE CONVERSION IN THE RAT AND MOUSE Chenyen Lai, Oscar Alvarez, Kristen Read, Don van Fossan,Christopher M Conner, Shannon Xaing-Ru Xu, Dale O. Cowley, Valentino Gantz, David R. Webb, Kurt Jarnagin URL: Doi:10.1101/2022.08.30.505951 URL: Doi:10.1101/2022.08.30.505951
URL: URL: Doi:10.1101/2022.08.30.505951 Donated to Rat Resource & Research Center
Proper citation: RRID:RGD_155260368 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155260367
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2022-10-06)
Alternate IDs: 155260367
Notes: rat strain in which the rat Cyp2e1 gene is humanized by insertion of a human CYP2E1 minigene (CYP2E1*) insert at the native start codon. The insert also included a CRISPR/Cas9 gene drive element to enable super-Mendelian inheritance of the modified allele when crossed to a rat expressing Cas9 in the germline. ROBUST AND EFFICIENT ACTIVE GENETICS GENE CONVERSION IN THE RAT AND MOUSE Chenyen Lai, Oscar Alvarez, Kristen Read, Don van Fossan,Christopher M Conner, Shannon Xaing-Ru Xu, Dale O. Cowley, Valentino Gantz, David R. Webb, Kurt Jarnagin URL: Doi:10.1101/2022.08.30.505951 URL: Doi:10.1101/2022.08.30.505951
URL: URL: Doi:10.1101/2022.08.30.505951 Donated to Rat Resource & Research Center
Proper citation: RRID:RGD_155260367 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155804258
Source Database: Rat Genome Database (RGD)
Genetic Background: inbred
Availability: Unknown
Alternate IDs: 155804258
Notes: inbred Brown Norway (BN) rat strain produced in the Rijswijk colony.
Proper citation: RRID:RGD_155804258 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=156430095
Source Database: Rat Genome Database (RGD)
Genetic Background: recombinant_inbred
Availability: Live Animals; Cryopreserved Embryo (as of 2023-10-24)
Alternate IDs: 156430095
Notes: This strain is derived from systematic breeding of the F2 generation between LE/Stm and F344/DuCrlj. This substrain was maintained at Medical College of Wisconsin RGD HRDP, contact HRDP
Proper citation: RRID:RGD_156430095 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=156430093
Source Database: Rat Genome Database (RGD)
Genetic Background: recombinant_inbred
Availability: Live Animals; Cryopreserved Embryo (as of 2023-10-24)
Alternate IDs: 156430093
Notes: This strain is derived from systematic breeding of the F2 generation between LE/Stm and F344/DuCrlj. This substrain was maintained at Medical College of Wisconsin RGD HRDP, contact HRDP
Proper citation: RRID:RGD_156430093 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=156430092
Source Database: Rat Genome Database (RGD)
Genetic Background: recombinant_inbred
Availability: Live Animals; Cryopreserved Embryo (as of 2023-10-24)
Alternate IDs: 156430092
Notes: This strain is derived from systematic breeding of the F2 generation between LE/Stm and F344/DuCrlj. This substrain was maintained at Medical College of Wisconsin RGD HRDP, contact HRDP
Proper citation: RRID:RGD_156430092 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=156430091
Source Database: Rat Genome Database (RGD)
Genetic Background: recombinant_inbred
Availability: Live Animals; Cryopreserved Embryo (as of 2023-10-24)
Alternate IDs: 156430091
Notes: This strain is derived from systematic breeding of the F2 generation between LE/Stm and F344/DuCrlj. This substrain was maintained at Medical College of Wisconsin RGD HRDP, contact HRDP
Proper citation: RRID:RGD_156430091 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=156430090
Source Database: Rat Genome Database (RGD)
Genetic Background: recombinant_inbred
Availability: Live Animals; Cryopreserved Embryo (as of 2023-10-24)
Alternate IDs: 156430090
Notes: This strain is derived from systematic breeding of the F2 generation between LE/Stm and F344/DuCrlj. This substrain was maintained at Medical College of Wisconsin RGD HRDP, contact HRDP
Proper citation: RRID:RGD_156430090 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=156430086
Source Database: Rat Genome Database (RGD)
Genetic Background: recombinant_inbred
Availability: Live Animals; Cryopreserved Embryo (as of 2023-10-24)
Alternate IDs: 156430086
Notes: This strain is derived from systematic breeding of the F2 generation between LE/Stm and F344/DuCrlj. This substrain was maintained at Medical College of Wisconsin RGD HRDP, contact HRDP
Proper citation: RRID:RGD_156430086 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155598601
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 155598601
Notes: CRISPR/Cas9 system was used to introduce a 58-base pair deletion in exon 6 in the rat Bmal1 gene of Crl:SD embryos. The deletion caused a premature stop codon in exon 6 resulting in a severe truncation of the Bmal1 protein. Contact MCW rat distribution at [email protected]
Proper citation: RRID:RGD_155598601 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155782885
Source Database: Rat Genome Database (RGD)
Genetic Background: congenic
Availability: Unknown
Alternate IDs: 155782885
Notes: SS/JrHsdMcwi were crossed with SS-3BN/Mcwi, rats from F1 were intercrossed and genotyped to select the congenic strain carrying introgressed chromosome 3 from SS to BN. Contact MCW rat distribution at [email protected] for availability.
Proper citation: RRID:RGD_155782885 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155782881
Source Database: Rat Genome Database (RGD)
Genetic Background: congenic
Availability: Unknown
Alternate IDs: 155782881
Notes: SS/JrHsdMcwi were crossed with SS-3BN/Mcwi, rats from F1 were intercrossed and genotyped to select the congenic strain carrying introgressed chromosome 3 from SS to BN. Contact MCW rat distribution at [email protected] for availability.
Proper citation: RRID:RGD_155782881 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155782880
Source Database: Rat Genome Database (RGD)
Genetic Background: congenic
Availability: Unknown
Alternate IDs: 155782880
Notes: SS/JrHsdMcwi were crossed with SS-3BN/Mcwi, rats from F1 were intercrossed and genotyped to select the congenic strain carrying introgressed chromosome 3 from SS to BN. Contact MCW rat distribution at [email protected] for availability.
Proper citation: RRID:RGD_155782880 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155631289
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 155631289
Notes: This mutant strain was generated by microinjecting CRISPRs/Cas9 system targeting rat Pde6b. Pde6b knock out rat was successfully created. Cyagen Biosciences Inc, Santa Clara, CA, USA
Proper citation: RRID:RGD_155631289 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155630633
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 155630633
Notes: The mutant rat was produced by injecting Crl:CD(SD) zygotes with gRNA +Cas9 ribonucleoprotein complex targeting exon 3 of rat Ctns. The founder of this strain possessed a 8-bp insertion which results in frameshift and pre-mature stop truncated protein.
Proper citation: RRID:RGD_155630633 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155631285
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 155631285
Notes: PAC671D9 (AF031078) DNA containing all the exons of human MECP2 was injected to SD embryos to generate MECP2 duplication rat.
Proper citation: RRID:RGD_155631285 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155782914
Source Database: Rat Genome Database (RGD)
Genetic Background: congenic
Availability: Unknown
Alternate IDs: 155782914
Notes: SS/JrHsdMcwi were crossed with SS-3BN/Mcwi, rats from F1 were intercrossed and genotyped to select the congenic strain carrying introgressed chromosome 3 from SS to BN. Contact MCW rat distribution at [email protected] for availability.
Proper citation: RRID:RGD_155782914 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155631278
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 155631278
Notes: This mutant strain was generated by electroporating rat zygotes with CRISPRs/Cas9 system targeting exon12 of rat Flna into Crl:SD embryo. This mutant strain carries P637Q knock in the gene.
Proper citation: RRID:RGD_155631278 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155782879
Source Database: Rat Genome Database (RGD)
Genetic Background: congenic
Availability: Unknown
Alternate IDs: 155782879
Notes: SS/JrHsdMcwi were crossed with SS-3BN/Mcwi, rats from F1 were intercrossed and genotyped to select the congenic strain carrying introgressed chromosome 3 from SS to BN. Contact MCW rat distribution at [email protected] for availability.
Proper citation: RRID:RGD_155782879 Copy
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