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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00055582
Source Database: WormBase (WB)
Affected Genes: WBGene00001803(lite-1)|WBGene00001804(gur-3)
Genomic Alteration: WBGene00001803(lite-1), WBGene00001804(gur-3)
Availability: unknown
Source References: EMPTY
Synonyms: otIs670 V; lite-1(ce314) gur-3(ok2245) X; flvIs17.
Notes: flvIs17 [tag-168::NLS::GCaMP7F + gcy-28.d::NLS::tagRFPt + ceh-36::NLS::tagRFPt + inx-1::tagRFPt + mod-1::tagRFPt + tph-1(short)::NLS::tagRFPt + gcy-5::NLS-;:tagRFPt + gcy-7::NLS::tagRFPt]. See description of strain OH15263 for full description of otIs670 NeuroPAL (Neuronal Polychromatic Atlas of Landmarks) transgene (Yemini E, et al. Cell. 2021 Jan 7;184(1):272-288.e11. PMID: 33378642). This strain can be used for pan-neuronal calcium imaging. Back-crossed 5x to MT21793 after transgene integration. Reference: Dag U, et al. bioRxiv 2023.01.15.524132; doi: https:|"Made_by: Steve Flavell"
Proper citation: RRID:WB-STRAIN:WBStrain00055582 Copy
http://www.wormbase.org/db/get?name=WBStrain00055627
Source Database: WormBase (WB)
Affected Genes: WBGene00001751(gst-3)|WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00001751(gst-3), WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: gst-3(hd7048[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) IV.
Notes: Homozygous viable. Deletion of 724 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: CTTTTAAAAGTTTATCGTTTTCAATATCCA; Right flanking sequence: TGGAGCAACTGGCCAGATGCACACTTATCT. sgRNA #1: GTTGATTAATGCCGAGTTGT; sgRNA #2: GCTCAGGAGACACAGACAGT. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: VH KO group"
Proper citation: RRID:WB-STRAIN:WBStrain00055627 Copy
http://www.wormbase.org/db/get?name=WBStrain00055626
Source Database: WormBase (WB)
Affected Genes: WBGene00001763(gst-15)|WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00001763(gst-15), WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: gst-15(hd7047[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) II.
Notes: Homozygous viable. Deletion of 2693 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: TTGCTCTTTTTGAGAATTCGATTGAGAAGA; Right flanking sequence: GACATTTCGGCGGCAGTCAACATTAATGAA. sgRNA #1: ATAAAGTAGACATCTCGAGC; sgRNA #2: CTTGTCAATACTCTCTCACG. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: VH KO group"
Proper citation: RRID:WB-STRAIN:WBStrain00055626 Copy
http://www.wormbase.org/db/get?name=WBStrain00055589
Source Database: WormBase (WB)
Affected Genes: WBGene00004187(prp-8)|WBGene00006805(unc-73)
Genomic Alteration: WBGene00004187(prp-8), WBGene00006805(unc-73)
Availability: unknown
Source References: EMPTY
Synonyms: unc-73(e936) I; prp-8(az117) III.
Notes: prp-8(az117) is a CRISPR-engineered R540K missense allele that suppress unc-73(e936) uncoordination through activation of an in-frame cryptic 5'ss. Reference: Cartwright-Acar CH, et al. Nucleic Acids Res. 2022 Nov 11;50(20):11834-11857. doi: 10.1093/nar/gkac991. PMID: 36321655.
Proper citation: RRID:WB-STRAIN:WBStrain00055589 Copy
http://www.wormbase.org/db/get?name=WBStrain00055622
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)|WBGene00011241(mpz-5)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54), WBGene00011241(mpz-5)
Availability: unknown
Source References: EMPTY
Synonyms: mpz-5(hd7014[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) IV.
Notes: Homozygous viable. Deletion of 1364 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: AAATAAGAAAGTTTTTTTTTTAATTTTTAA; Right flanking sequence: ATGCCCGTTGCCATGCCGTTGTTCCGATAG. sgRNA #1: AAAGCTGTCTCACAAAGTAA; sgRNA #2: GAGGAGGAAAGGAATTAGGG. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: VH KO group"
Proper citation: RRID:WB-STRAIN:WBStrain00055622 Copy
http://www.wormbase.org/db/get?name=WBStrain00055625
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006701(ubc-1)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006701(ubc-1), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: ubc-1(hd7046[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) IV.
Notes: Homozygous viable. Deletion of 2049 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: AGGAGTTAGGTTATCAATATGACGACGCCC; Right flanking sequence: TGGAAATTGAAGAAATTGCTGCTCCAGGAG. sgRNA #1: CTCATCAAACGTCTACGGCT; sgRNA #2: CGCAGTGCTCAAGGATGACG. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: VH KO group"
Proper citation: RRID:WB-STRAIN:WBStrain00055625 Copy
http://www.wormbase.org/db/get?name=WBStrain00055624
Source Database: WormBase (WB)
Affected Genes: WBGene00000287(cal-3)|WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00000287(cal-3), WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: cal-3(hd7045[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) IV.
Notes: Homozygous viable. Deletion of 6270 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: AACGTACGTTATACGTAATAGAAGCACGTG; Right flanking sequence: CTCGGGGCGAATTCAAATAAAGATGCGGCT. sgRNA #1: CGTAATAGAAGCACGTGAGA; sgRNA #2: CGCAATTTGATCACACTCTC. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: VH KO group"
Proper citation: RRID:WB-STRAIN:WBStrain00055624 Copy
http://www.wormbase.org/db/get?name=WBStrain00055591
Source Database: WormBase (WB)
Affected Genes: WBGene00006805(unc-73)|WBGene00012896(snrp-200)
Genomic Alteration: WBGene00006805(unc-73), WBGene00012896(snrp-200)
Availability: unknown
Source References: EMPTY
Synonyms: unc-73(e936) I; snrp-200(az123) II.
Notes: az123 is an N18K missense allele altering 5' cryptic splicing usage. az123 is a CRISPR-induced mutation mimicking a known suppressor of unc-73(e936). Reference: Cartwright-Acar CH, et al. Nucleic Acids Res. 2022 Nov 11;50(20):11834-11857. doi: 10.1093/nar/gkac991. PMID: 36321655.
Proper citation: RRID:WB-STRAIN:WBStrain00055591 Copy
http://www.wormbase.org/db/get?name=WBStrain00055691
Source Database: WormBase (WB)
Affected Genes: WBGene00006784(unc-49)
Genomic Alteration: WBGene00006784(unc-49)
Availability: unknown
Source References: EMPTY
Synonyms: unc-49(e407) III; hpIs592; ljIs131.
Notes: hpIs592 [ttr-39p::Chrimson::wCherry + lin-15(+)]. ljIs131 [myo-3p::GCaMP3::UrSL2::tagRFP-T]. Shrinker. Body relaxation upon green light illumination with ATR. Reference: Lu Y, et al. 2022. Current Biology (In Press).
Proper citation: RRID:WB-STRAIN:WBStrain00055691 Copy
http://www.wormbase.org/db/get?name=WBStrain00055694
Source Database: WormBase (WB)
Affected Genes: WBGene00006784(unc-49)|WBGene00022675(gbb-2)
Genomic Alteration: WBGene00006784(unc-49), WBGene00022675(gbb-2)
Availability: unknown
Source References: EMPTY
Synonyms: unc-49(e407) III; gbb-2(tm1165) IV; hpIs592; ljIs131.
Notes: hpIs592 [ttr-39p::Chrimson::wCherry + lin-15(+)]. ljIs131 [myo-3p::GCaMP3::UrSL2::tagRFP-T]. Shrinker. Red fluorescence in D motor neurons. Reference: Lu Y, et al. 2022. Current Biology (In Press).
Proper citation: RRID:WB-STRAIN:WBStrain00055694 Copy
http://www.wormbase.org/db/get?name=WBStrain00055699
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: hpIs596; hpIs268.
Notes: hpIs596 [acr-2(s)p::Chrimson::wCherry + lin-15(+)]. hpIs268 [unc-25p::GCaMP3si::SL2 wCherry + lin-15(+)]. Unc (linked to hpIs596). Green and red fluorescence in D-motor neurons. Very weak red fluorescence in A and B motorneurons. Reference: Lim MA, et al. eLife 2016;5:e19887. doi: 10.7554/eLife.19887. PMID: 27855782.
Proper citation: RRID:WB-STRAIN:WBStrain00055699 Copy
http://www.wormbase.org/db/get?name=WBStrain00055732
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: hpIs615; hpIs365.
Notes: hpIs615 [acr-2(s)p::Arch::wCherry + lin-15(+)]. hpIs365 [unc-25p::GCaMP3::wCherry + lin-15(+)]. RFP expression in motor neurons. A and B motor neurons are inhibited and body relaxes upon illumination with green light. Reference: Lu Y, et al. 2022. Current Biology (In Press).
Proper citation: RRID:WB-STRAIN:WBStrain00055732 Copy
http://www.wormbase.org/db/get?name=WBStrain00055735
Source Database: WormBase (WB)
Affected Genes: WBGene00017641(csr-1)
Genomic Alteration: WBGene00017641(csr-1)
Availability: unknown
Source References: EMPTY
Synonyms: fjSi1 II; csr-1(fj54) IV.
Notes: fjSi1 [2FLAG::csr-1 + Cbr-unc-119(+)] II. Single-copy insertion in the MosSCI locus ttTi5605 (LG II). The 2FLAG::csr-1 transgene was designed to express proteins with a double FLAG tag instead of the N169 of CSR-1a and N6 of CSR-1b. The linker sequence between the two FLAG tags has a NotI site. The insertion can be checked by PCR with the following primers: CACACTCGATTCTACGCCAA (at the 3'-side of csr-1) and ATCGGGAGGCGAACCTAACTG (near ttTi5605 on LG II). Reference: Tabara H, et al. (2023) A small RNA system ensures accurate homologous pairing and unpaired silencing of meiotic chromosomes. EMBO J, e105002.
Proper citation: RRID:WB-STRAIN:WBStrain00055735 Copy
http://www.wormbase.org/db/get?name=WBStrain00055734
Source Database: WormBase (WB)
Affected Genes: WBGene00006762(unc-25)
Genomic Alteration: WBGene00006762(unc-25)
Availability: unknown
Source References: EMPTY
Synonyms: unc-25(e156) III; hpIs673; ljIs131.
Notes: hpIs673 [rgef-1p::Chrimson::UrSL2::wCherry + lin-15(+)]. ljIs131 [myo-3p::GCaMP3::UrSL2::tagRFP-T]. Shrinker. All neurons are marked with red fluorescence. Pan-neuronal activation and muscle contraction upon green light illumination with ATR. Reference: Lu Y, et al. 2022. Current Biology (In Press).
Proper citation: RRID:WB-STRAIN:WBStrain00055734 Copy
http://www.wormbase.org/db/get?name=WBStrain00055685
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: hpIs740.
Notes: hpIs740 [twk-40(s)p::GCaMP6s::wScarlet + lin-15(+)]. GCaMP and RFP expression in AVA, AVB, AVE and DVA. Reference: Lu Y, et al. 2022. Current Biology (In Press).
Proper citation: RRID:WB-STRAIN:WBStrain00055685 Copy
http://www.wormbase.org/db/get?name=WBStrain00055684
Source Database: WormBase (WB)
Availability: unknown
Source References: PMID:38598630
Synonyms: hpIs758.
Notes: hpIs758 [rig-3p::LoxP::eBFP::LoxP::Chrimson::wCherry + twk-40(s)p::Cre + myo-2p::wCherry]. Pick animals with red fluorescence to maintain. Weak myo-2 and AVA Cherry expression. hpIs758 is a spontaneous insertion of hpEx4080. Reference: Lu Y, et al. 2022. Current Biology (In Press).
Proper citation: RRID:WB-STRAIN:WBStrain00055684 Copy
http://www.wormbase.org/db/get?name=WBStrain00055687
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: hpIs717; ljIs131.
Notes: hpIs717 [acr-2(s)p::LoxP::eBFP::LoxP::Chrimson::wCherry + unc-17p::Cre + lin-15(+)]. ljIs131 [myo-3p::GCaMP3::UrSL2::tagRFP-T]. Red fluorescence in motor neurons. Reference: Lu Y, et al. 2022. Current Biology (In Press).
Proper citation: RRID:WB-STRAIN:WBStrain00055687 Copy
http://www.wormbase.org/db/get?name=WBStrain00055686
Source Database: WormBase (WB)
Affected Genes: WBGene00006762(unc-25)
Genomic Alteration: WBGene00006762(unc-25)
Availability: unknown
Source References: EMPTY
Synonyms: unc-25(e156) III; ljIs131; hpIs758.
Notes: ljIs131 [myo-3p::GCaMP3::UrSL2::tagRFP-T]. hpIs758 [rig-3p::LoxP::eBFP::LoxP::Chrimson::wCherry + twk-40(s)p::Cre + myo-2p::wCherry]. Pick animals with red fluorescence to maintain. Shrinker. RFP expression in AVA and a few other neurons. Reversal upon green light illumination with ATR. hpIs758 is a spontaneous insertion of hpEx4080. Reference: Lu Y, et al. 2022. Current Biology (In Press).
Proper citation: RRID:WB-STRAIN:WBStrain00055686 Copy
http://www.wormbase.org/db/get?name=WBStrain00055683
Source Database: WormBase (WB)
Affected Genes: WBGene00006762(unc-25)
Genomic Alteration: WBGene00006762(unc-25)
Availability: unknown
Source References: EMPTY
Synonyms: unc-25(e156) III; hpIs593; ljIs131.
Notes: hpIs593 [ttr-39p::Chrimson::wCherry + lin-15(+)]. ljIs131 [myo-3p::GCaMP3::UrSL2::tagRFP-T]. Shrinker. D motor neurons are marked with red fluorescence. No behavioral change upon green light illumination with ATR. Reference: Lu Y, et al. 2022. Current Biology (In Press).
Proper citation: RRID:WB-STRAIN:WBStrain00055683 Copy
http://www.wormbase.org/db/get?name=WBStrain00055726
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: hpIs625; ljIs131.
Notes: hpIs625 [ttr-39p::Arch::wCherry + lin-15(+)]. ljIs131 [myo-3p::GCaMP3::UrSL2::tagRFP-T]. Reference: Lu Y, et al. 2022. Current Biology (In Press).
Proper citation: RRID:WB-STRAIN:WBStrain00055726 Copy
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