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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: disD-1/disD-1 (disD-1, IV)
Affected Genes: DISD (disorganized-D)
Source References: PMID:18658256
Notes: Disorganized ciliary rows at 39-40 degrees C. Straightforward t.s. expression of disorganized phenotype,cell division normal.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01222 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (dcl1-?[3’neo3,HAc]/dcl1-?[3’neo3,HAc], VII)
Affected Genes: DCL1 (TTHERM_00284230)
Genomic Alteration: Macronucleus: Mac-DCL1 was completely replaced with DCL1-HA- with neo3 in 3’ flanking region
Source References: PMID:15598983
Notes: Mac-DCL1 was completely replaced with DCL1-HA-neo3, CU428. DCL1 = SAI 2, the original name.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02832 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hht2[3’ neo2,FLAGc]; pm-r, VII)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: c-terminal HHT2 FLAG tagged
Source References: PMID:16908532, PMID:9343391
Notes: Don’t know if complete replacement.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02313 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT (TTHERM_00570560, TTHERM_00189180, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo3 KO of major H3s and HHT3
Source References: PMID:16908532, PMID:15701804
Notes: homozygous, Neo cassette knockout before we knew there was HHT4
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01865 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (htax[S134A,3’neo2]; pm-r, II)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Macronucleus: HTAX has mutation S134A with neo2 cassette in 3’ flank
Source References: PMID:17242195
Notes: Complete somatic transformant with hta1s134-neo2 replacing the HTA1(HTAX) gene in mac of B2086.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01903 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Proper citation: RRID:TSC_SD01194 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaH-1/cdaH-1 (cdaH-1; cdaH, IV)
Affected Genes: CDAH (cell division arrest H)
Notes: No detailed phenotypic studies were done on this clone. It is the most recent cdaH-1 homozygote and is most likely to yield acceptable breeding results
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01593 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Nulli 4 (MPR1; mp-s, VII)
Proper citation: RRID:TSC_SD01079 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (twi1[3’neo3, FLAG-HAn]; pm-r, VI)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Macronucleus: FLAG-HA tagged TWI1 with neo3 replaced all TWI1
Source References: PMID:12297043
Notes: All somatic TWI1 was replaced by FLAG-HA-TWI1 construct. CU427 background.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02608 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 4 (CHX1; pmr1-1; cy-s, pm-r, II)
Proper citation: RRID:TSC_SD01083 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (HHF1;HHT1;HHF2/HHT2; mpr1-1; mp-r, ?)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes
Source References: PMID:15701804
Notes: Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It mates with gl∆H3/H4 #7 strain.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01361 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: TWI1/twi1[∆::neo3] (?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Micronucleus: Neo3 into TWI1, heterozygous
Source References: PMID:12297043
Notes: Heterozygous heterokaryon of ∆TWI1 progeny of hehe∆TWI1 F3-1 x CU427; assorted to pm-s; can mate with c5-2, c6-1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02850 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-1[K350M]/btu1-1[K350M] (btu1-1[K350M]/btu1-?[MTT1p,,∆,(::GTU1,T146G),HAc]; tax-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Macronucleus: GTU1 with mutation T146G and c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
Source References: PMID:12356864, PMID:16344310
Notes: pT146G HA somatic transformant in CU522, partial ? replacement of btu1(K350M) gene,GTU1 (T146G) with HA tag under control of MTT1 promoter. #1 and #5 are two separate single cell lines.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01365 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (cnjB[5’neo3,HAn]; pm- r, HA tagged CnjB, II)
Affected Genes: nnjB (TTHERM_01091290)
Genomic Alteration: Macronucleus: cnjB[5’neo3, HAn]
Source References: PMID:19596782
Notes: Somatic HA-tagged Cnj-B. fully assorted. Mates with HA- CNB CU428 #2.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01890 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[3’neo2, HA-His-n]; Mac transformant, HA-His tagged hv2 gene
pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal HA-His- tag of HHT3, not sure if complete replacement
Source References: PMID:9343391, PMID:16908532
Notes: HA-His-tagged hv2 transformant. Checked by western blot. HA-His-hv2 is expressed.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01891 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cna1[∆::neo2]/cna1[∆::neo2] (?)
Affected Genes: CNA1 (TTHERM_00146340)
Genomic Alteration: Micronucleus: Neo2 into CNA1
Source References: PMID:16738316
Notes: Germline CenPA KO heterokaryon. CenPA coding is replaced by neo2. Star side.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01931 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pgp1[∆?::neo3]/pgp1[∆?::neo3] (pm-, ?)
Affected Genes: PGP1
Genomic Alteration: Micronucleus: neo3 into PGP1 locus
Source References: PMID:17189490
Notes: Possible poly glycine protein gene was knocked out with neo3 cassette. This is a PPG1 KO heterokaryon with homozygous mic KO and mac assorted to wt.This strain can mate with PPG1∆4 cells. Final name of gene is PGP1when published.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02501 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/GAL1-1; chx1-1/CHX1-1 (gal1-1; chx1-1; gal-r; cy-r, V)
Notes: Generated by crossing SB1969 and SB210E. Progeny were selected as being cy-r and gal-r.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01657 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2] /gtu1[GTU1,T270A,Y272A ]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T270A, Y272A. Not sure if complete replacement.
Source References: PMID:16344310, PMID:12356864
Notes: pT270Y272 rescued GTUKO 5x6 progeny having mutation T270A,Y272A in GTU1 in GTU1 locus. Cell line #5 can be crossed with cell line #6.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02193 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, HAc(::GTU1, T270A, Y272A)]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations T270A, Y272A anc c-terminus HA tag into the MTT1 locus, partial replacement.
Source References: PMID:12356864, PMID:16344310
Notes: pR285D291 rescued GTUKO 5x6 progeny having mutation @ T270,Y272-AA in GTU1-1 gene, driven by MTT1 promoter. Needs Cd to induce. Has HA tag at c-terminus.
#3 can be crossed with #6.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02194 Copy
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