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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=10401848
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 10401848
Notes: ZFN mutant founders were backcrossed with WKY/Cruk to get heterozygous offsprings which were intercrossed and offsprings maintained as homozygous and heterozygous breeders. The resulting mutation 77 has a stop codon, 15 amino acids from the ZFN-binding site, that resulted in a 490 amino acids (54 kDa) truncated protein, 198 amino acids smaller than the WT protein.
Proper citation: RRID:RGD_10401848 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=11531104
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 11531104
Notes: The heterozygous ZFN mutant rats were produced by backcrossing mutant founders with iNP/Iusm. Genotyping of the offspring was carried out by PCR amplification of a 26-bp deleted fragment, including 6 bp in intron 1 and 20 bp in exon 2.
Proper citation: RRID:RGD_11531104 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=11531106
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 11531106
Notes: The homozygous Npy mutant rats were produced by intercrossing the heterozygous ZFN mutants (iNP-Npyem6Sage+/- /Iusm) to produce homozygous and heterozygous offspring. Genotype of the homozygous offspring was confirmed by PCR amplification of a 26-bp deleted fragment, including
6 bp in intron 1 and 20 bp in exon 2.
Proper citation: RRID:RGD_11531106 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=10054239
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Cryopreserved Sperm (as of 2018-10-22)
Alternate IDs: 10054239
Notes: CRISPR/Cas9 system was used to introduce a 2-bp insertion mutation in the exon1 of the Abcd1 gene of DA/OlaHsd rat embryos. Contact MCW rat distribution at [email protected]
Proper citation: RRID:RGD_10054239 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=9835401
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 9835401
Notes: The Zucker rats from Charles River (ZDF-Leprfa/Crl) carry the Leprm1Rll allele that has substitution of a nucleotide at 880 (A-C) results in Gln-Pro at position 269
Proper citation: RRID:RGD_9835401 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=11531094
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 11531094
Notes: These ZFN mutant rats were produced by injecting zinc finger nuclease targeting rat F8 into Sprague Dawley embryos. A premature translation stop in rat F8 was created in the mutant founders carrying a 13-bp deletion in exon 16. These mutant founders were backcrossed with Sprague Dawley to produce heterozygous offspring, which were then intercrossed to produce homozygous and heterozygous offspring.
Proper citation: RRID:RGD_11531094 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=10054287
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Live Animals; Cryopreserved Sperm (as of 2017-01-26)
Alternate IDs: 10054287
Notes: CRISPR/Cas9 system was used to introduce a mutation in the Adora2a gene of SS/JrHsdMcwi rat embryos. The resulting mutation is a 98-bp deletion in the Adora2a gene. Contact MCW rat distribution at [email protected]
Proper citation: RRID:RGD_10054287 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=10054299
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Cryopreserved Sperm (as of 2021-11-03)
Alternate IDs: 10054299
Notes: CRISPR/Cas9 system was used to introduce a 16-bp deletion in the Btg2 gene of SS.BN-(D13Rat25-rs106935835)/Mcwi rat embryos. Contact MCW rat distribution at [email protected]
Proper citation: RRID:RGD_10054299 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=11040985
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Extinct (as of 2017-05-02)
Alternate IDs: 11040985
Notes: By CRISPR/Cas9 system, mutation was introduced in dystrophin (Dmd) gene of Wistar-Imamichi rat: Deletion of exon3 to exon16 (a part of intron 3 remain). This strain died out in 2015. National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_11040985 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=11553914
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Cryopreserved Sperm (as of 2017-01-26)
Alternate IDs: 11553914
Notes: ZFN system was used to introduce a mutation in the Shc1 gene of SS/JrHsdMcwi rat embryos. The resulting mutation is a 13-bp deletion in Exon 2 of theShc1 gene. Contact MCW rat distribution at [email protected]
Proper citation: RRID:RGD_11553914 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=11040918
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Cryopreserved Sperm
Alternate IDs: 11040918
Notes: WTC-furue rat was found in a colony of WTC.ZI-Atrn
Proper citation: RRID:RGD_11040918 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=11040519
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 11040519
Notes: ZFN mutant founders were backcrossed with SHR/NCrl to get heterozygous offsprings which were intercrossed and offsprings maintained as homozygous and heterozygous breeders.
Proper citation: RRID:RGD_11040519 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=11040916
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Cryopreserved Sperm
Alternate IDs: 11040916
Notes: Zinc-finger nucleases (ZFNs) method taregting exon 5 of Zeb2 gene (AGCCAAAGCTTGCCTCCA and GACTACTGACTCAAG) was used; This strain has a 5-bp deletion (cagag) and a 2-bp insertion (tc) in exon7 of Nrf2 gene, resulting in a deletion of Glutamine(Q)541. Background strain: F344/Stm National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_11040916 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=11553904
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Cryopreserved Sperm (as of 2017-01-26)
Alternate IDs: 11553904
Notes: ZFN system was used to introduce a mutation in the Shc1 gene of SS/JrHsdMcwi rat embryos. The resulting mutation is a 27-bp deletion in Exon 1 of the Shc1 gene. Contact MCW rat distribution at [email protected]
Proper citation: RRID:RGD_11553904 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=11040948
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Cryopreserved Sperm (as of 2017-06-08)
Alternate IDs: 11040948
Notes: This strain was established by Zinc-finger nucleases (ZFNs) method taregting rat Prkdc gene (227-bp deletion) and Il2rg gene (332-bp deletion). Background strain: F344/Stm National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_11040948 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=11553894
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Cryopreserved Sperm (as of 2021-11-03)
Alternate IDs: 11553894
Notes: ZFN system was used to introduce a mutation in the Tpcn2 gene of SS/JrHsdMcwi rat embryos. The resulting mutation is a 9-bp deletion in Exon 4 of theTpcn2 gene. Contact MCW rat distribution at [email protected]
Proper citation: RRID:RGD_11553894 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=11040954
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Cryopreserved Embryo
Alternate IDs: 11040954
Notes: LEA rats were provided from Kyoto Bioresource Center in 2006. Then LEA rat ES cells having Oct4-Venus gene and Oct4-Venus Tg rat strain (LEA-Tg(Pou5f1-YFP*)3Ncco) were established. National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_11040954 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=10401843
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 10401843
Notes: This strain was produced by injecting ZFNs targeted sequence into WKY/Cruk rat embryos. The resulting mutation 77 has a stop codon, 15 amino acids from the ZFN-binding site, that resulted in a 490 amino acids (54 kDa) truncated protein, 198 amino acids smaller than the WT protein.
Proper citation: RRID:RGD_10401843 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=9835403
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 9835403
Notes: The WDF colony at Vassar College (WDF/Vc) carry the Leprm1Rll allele that has substitution of a nucleotide at 880 (A-C) results in Gln-Pro at position 269
Proper citation: RRID:RGD_9835403 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=11531091
Source Database: Rat Genome Database (RGD)
Genetic Background: mutant
Availability: Unknown
Alternate IDs: 11531091
Notes: The homozygous F8 mutant rats were produced by intercrossing the heterozygous ZFN mutants (SD-F8em1Sage+/-/Novo) to produce homozygous and heterozygous offspring. Genotype of the homozygous offspring was confirmed by PCR amplification of the deletion fragment which caused a premature translation stop.
Proper citation: RRID:RGD_11531091 Copy
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